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Method for preparing duck oil diglyceride

A technology of diglyceride and duck oil, which is applied in the field of immobilized lipase to catalyze the preparation of duck oil diglyceride, can solve the problems of pollution, a large number of chemical reagents, and low product purity, and achieve reduced production costs, reduced environmental pollution, and guaranteed The effect of food safety

Active Publication Date: 2016-04-20
QINGDAO RICHEN FOODS
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AI Technical Summary

Problems solved by technology

However, due to the lack of specificity in the reaction, the resulting product is a mixture of 1,2-diglyceride and 1,3-diglyceride, usually in a ratio of 7:3 to 6:4
Therefore, the method usually cannot predict where the fatty acid will be incorporated in the final product
Although 1,3-DG with a special structure can also be produced through specific chemical reactions, protective agents are required, the reaction steps are complicated and lengthy, and a large amount of chemical reagents or organic solvents are required, resulting in serious pollution, which is not expected by the food and pharmaceutical industries , it is not suitable from the perspective of clean production and green environmental protection requirements
2) Hydrolysis method: Most of the currently disclosed methods of hydrolyzing fat to produce diglycerides are carried out under conditions of high temperature and high pressure (patent CN101768076A), which easily oxidizes unsaturated fatty acids, consumes a lot of energy, and has high production costs , and is easy to evaporate and cause pollution, which is not conducive to large-scale production
Using natural lipase as a catalyst to catalyze oil hydrolysis or glycerol alcoholysis to synthesize diacylglycerol has become the most industrially promising preparation method due to the use of efficient biocatalysts; however, this enzyme cannot be completely combined with the product (diglycerol) Separation, the product purity is low, and food safety cannot be guaranteed; in addition, this process cannot enable the enzyme to be recycled and reused

Method used

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  • Method for preparing duck oil diglyceride
  • Method for preparing duck oil diglyceride
  • Method for preparing duck oil diglyceride

Examples

Experimental program
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Effect test

preparation example Construction

[0027] 1) Preparation of mixed fatty acids: put 200g of duck oil into a 1000mL beaker, add distilled water to 440mL, and place in a constant temperature water bath at 87°C for 1.5h. Then transfer to a separatory funnel to remove the lower layer, and repeat the above operation. Then add a certain amount of 1mol / L NaOH solution, hydrolyze at a constant temperature of 87°C, wash with NaCl to remove glycerol, acidify to pH 2-3 with 10% HCl, and release free fatty acids.

[0028] 2) Preparation of duck oil diglycerides: Put glycerol and duck oil mixed fatty acids in a ratio of 1:0.5-3 into an Erlenmeyer flask, add 0.5%-3% of immobilized Candida antarctica lipase B, Then place it on a shaker, set the temperature at 30°C to 70°C, and set the reaction time at 2 to 12 hours. After the reaction, centrifuge the above reaction solution in a high-speed centrifuge at 4000r / min for 20 minutes to separate the enzyme. The obtained supernatant is a high-purity diglyceride mixture. The content...

Embodiment 1

[0030] Embodiment 1: the preparation of duck oil diglyceride

[0031] Put glycerin and duck oil mixed fatty acid into the Erlenmeyer flask according to the ratio of 1:0.5-3, add 0.5%-3% immobilized Candida antarctica lipase B, then place it on the shaker, set the temperature as 30 ℃ ~ 70 ℃, set the reaction time to 2 ~ 12h, after the reaction, centrifuge the above reaction solution in a 4000r / min high-speed centrifuge for 20min, then the enzyme can be separated, and the obtained supernatant is high-purity glycerol Diester mixture. The content of the obtained diglyceride mixture was determined by high performance liquid chromatography.

[0032] The optimization steps of above-mentioned reaction conditions are as follows:

[0033] (1) Screening of the amount of enzyme added: the substrate ratio (glycerol: mixed fatty acid) was set to 1:2, the reaction temperature was 50°C, the reaction time was 10h, and the amount of enzyme added was 0.5%, 1%, and 1.5%, respectively, 2%, 2.5%...

Embodiment 2

[0051] Example 2: Enzymatic hydrolysis of duck oil to prepare diglyceride optimal process conditions screening regression test

[0052] Put the mixed fatty acid and glycerol into the Erlenmeyer flask according to the ratio of substrate ratio (glycerol: mixed fatty acid) 1:2.02, place it in a constant temperature shaker, and set the reaction temperature at 44°C, and add 1.65% immobilized Antarctica Trichoceria lipase B, the reaction time is 9.1h. After the reaction, centrifuge the enzymatic solution in a high-speed centrifuge for 10 minutes to separate the enzyme. The supernatant is a mixture of high diglycerides, and the middle layer is immobilized lipase , Determination of the formation rate of diglycerides by liquid chromatography.

[0053] Through 5 verification tests, it was found that the production rate of diglyceride obtained under this condition was 93.25%, 94.09%, 93.88%, 94.22%, 94.17%, and the average extraction rate was 93.92%, which was basically consistent with t...

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Abstract

The invention relates to a method for preparing duck oil diglyceride through catalysis of immobilized lipase. The method for preparing the duck oil diglyceride through the catalysis of the immobilized lipase comprises the following steps: firstly utilizing an alkali hydrolysis method to prepare fatty acid mixture, mixing the fatty acid mixture with glycerinum, and generating a catalytic reaction by utilizing the immobilized lipase; by utilizing an experiment of four single factors, including enzyme dosage, specific substrate, enzymolysis temperature and enzymolysis time, and response surface analysis method, optimal conditions for preparing the duck oil diglyceride from the immobilized lipase are obtained. When the duck oil diglyceride is prepared from the immobilized lipase, enzyme and product diglyceride can be completely separated, so that no enzyme residue exists in the product, the purity of the product is high, and the safety of foods is guaranteed. Furthermore, the selected immobilized lipase can be recycled and reutilized, so that the production costs are reduced, and environmental pollution is reduced. The method for preparing the duck oil diglyceride, which is provided by the invention, creates a new way for comprehensive utilization of duck oil side-products, the additional value of the duck oil can be largely increased, and the method is a green creative project with the important development value.

Description

technical field [0001] The invention belongs to the technical field of diglyceride preparation, and in particular relates to a method for preparing duck oil diglyceride by catalyzing immobilized lipase. Background technique: [0002] The content of polyunsaturated fatty acid (PUFA) in duck oil is higher, and the content of monounsaturated fatty acid (MUFA) is higher than that of lard, peanut oil, soybean oil, corn oil, and olive oil. At present, there are few studies on duck oil, and most duck oil is used as feed, with low economic value. The present invention intends to use duck oil as a fatty acid donor to catalyze the preparation of diglyceride, so as to broaden the utilization of duck oil and increase the added value of fat produced in duck processing. [0003] Diacylglycerol is a product obtained by esterification of glycerol (glycerol) with two fatty acids. It is divided into two isomers, 1,3-diglyceride and 1,2-diglyceride. Studies have shown that diacylglycerol (D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6445
Inventor 王宝维贺可琳葛文华张名爱岳斌
Owner QINGDAO RICHEN FOODS
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