A circulatory system for culturing high-abundance isotope carbon and nitrogen double-labeled plant samples
A circulatory system and isotope technology, which is applied in the field of circulatory system for cultivating high-abundance isotope carbon and nitrogen double-labeled plant samples, can solve the problem of inability to achieve isotope carbon and nitrogen double-labeling cyclic culture, and cannot obtain high-abundance double-labeled plants. problems such as samples, to achieve the effect of improving isotope utilization, improving water utilization, and reducing labeling costs
Active Publication Date: 2022-06-21
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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[0008] The invention provides a circulation system for cultivating high-abundance isotope carbon and nitrogen double-labeled plant samples, which is used to solve the problem that the existing isotope labeling technology cannot realize the circulation culture of isotope carbon and nitrogen double-labeling, and cannot obtain high-abundance double-labeled plants sample problem
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[0030]In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the related drawings. The preferred embodiments of the invention are shown in the accompanying drawings. The above are only preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields , are similarly included in the scope of patent protection of the present invention.
[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terms used herein in the description of the present invention are for the purpose of describing specif...
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The invention relates to the technical field of isotope labeling, in particular to a circulation system for cultivating high-abundance isotope carbon and nitrogen double-labeled plant samples, including a closed culture room, CO 2 Syringe, gas balance device, nutrient solution injector and nutrient solution circulation system; the airtight culture room includes a transparent cover and a base, and a semiconductor condensation dehumidification device is installed in the transparent cover, and the transparent cover is connected to the CO 2 Syringes and gas balance devices; culture basins, liquid storage chambers, outflow peristaltic pumps, ultraviolet sterilizers, circulating peristaltic pumps, and confluence pools are connected in sequence to form a circulation loop of the nutrient solution circulation system; the confluence pool is connected to the semiconductor condensation dehumidification device and the nutrient solution A syringe; a culture basin and a liquid storage chamber are arranged in the base, the top of the culture basin is connected with a transparent cover, and quartz sand is arranged in the culture basin. The invention can mark carbon and nitrogen isotopes at the same time, and realize their recycling, without ordinary 12 C and 14 N pollution, and high-abundance carbon and nitrogen isotope double-labeled plants.
Description
technical field [0001] The invention relates to the technical field of isotope labeling, in particular to a circulatory system for culturing high-abundance isotope carbon and nitrogen double-labeled plant samples. Background technique [0002] In recent years, the cycle process of carbon and nitrogen elements contained in crop straw in soil has become one of the research hotspots in plant nutrition and soil science. Isotope tracing technology is a key technology to study the decomposition and transformation process of crop straw in soil, which can effectively reveal the release law of straw elements and the bioavailability of organic nutrients. by the stable isotope nitrogen ( 15 N) The labeled crop straw was applied to the soil, and the amount of 15 Changes in N abundance can accurately calculate straw 15 The amount of N nutrient transferred to soil and plants, and the proportion of loss through gas, leaching, etc. Similarly, using the stable isotope carbon ( 13 C) Tra...
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IPC IPC(8): A01G31/02A01G7/02
CPCA01G7/02A01G31/02Y02P60/21
Inventor 艾超周卫
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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