Device and method for continuous quantification of labeled plants

A plant and labeling technology, applied in the field of devices for continuous quantitative labeling of plants, can solve problems such as inability to distribute isotopes uniformly, inability to distinguish isotopic differences, and inability to provide all plant information.

Inactive Publication Date: 2016-04-06
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

[0004] However, because the pulse labeling technique is a one-time addition of high-abundance isotopes, the labeling only lasts for several hours, so it can only trace the migration and transformation of substances in the soil-plant-atmosphere system in a short period of time, but cannot provide information on the entire growth of plants. All information for the period
In addition, pulse-labeling technology cannot achieve uniform distribution of isotopes in the whole soil-plant-atmosphere system, and cannot distinguish differences in isotopic composition between different biomarker compounds

Method used

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  • Device and method for continuous quantification of labeled plants

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[0029] specific implementation plan

[0030] The combination provided by the present invention 13 C-CO 2 and 15 N-KNO 3 Devices for continuous quantitative labeling of plants, including: plant assimilation chambers, plant potting systems, 13 C-CO 2 Abundance control system, drip irrigation system, temperature control system, humidity control system, atmospheric pressure control system.

[0031] The feature of the plant assimilation chamber is that the plant assimilation chamber is a sealed isotope labeling box made of transparent plexiglass plates (light transmittance greater than 85%), and the dimensions of length * width * height are 60cm * 40cm * 100cm.

[0032] The characteristics of the plant potting system are: the PVC pot is made of plexiglass plate, and the size of length x width x height is 30cm x 20cm x 45cm; the PVC pot is filled with soil, and the plants are cultivated on the soil; a PVC partition is installed above the PVC pot, The interior of the PVC pot is...

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Abstract

Provided is a device combining 13C-CO2 and 15N-KNO3 for continuously and quantitatively labeling plants. The device is composed of a plant assimilation chamber, a plant potting system, a 13C-CO2 abundance control system, a drip irrigation system, a temperature control system, a humidity control system and an atmospheric pressure control system. The invention further discloses a method for continuously and quantitatively labeling the plants through the device. Continuous 13C labeling is carried out on the plants through the 13C-CO2 abundance control system, continuous 15N labeling is carried out on the plants through the drip irrigation system, and temperature, humidity and atmospheric pressure in the plant assimilation chamber can be stable in the isotope labeling process through the temperature control system, the humidity control system and the atmospheric pressure control system. Continuous labeling can be carried out on the plants in the whole growing period of the plants, and the two stable isotopes of 13C and 15N can be labeled at the same time.

Description

technical field [0001] The present invention relates to a device for continuously quantifying and marking plants, and more particularly relates to a combined 13 C-CO 2 and 15 N-KNO 3 Device for continuous quantification of labeled plants. [0002] The present invention also relates to a method for continuously quantifying and marking plants using the above-mentioned device. Background technique [0003] The material and energy exchange and information transfer process in the soil-plant-atmosphere continuum is the core content of the terrestrial ecosystem process, and the carbon and nitrogen cycle has become a hot spot in global change research in the past decade. The stable isotope tracer technique is an important means to quantitatively study the carbon and nitrogen cycle in the soil-plant-atmosphere continuum. 13 C and 15 N isotope labeling technology can provide valuable information for studying the coupling mechanism of carbon and nitrogen processes in soil-plant-a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00G01N33/60
Inventor 檀文炳席北斗何小松高如泰李丹
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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