The invention belongs to the global change- terrestrial ecosystem-greenhouse gas change field, in particular to a method of measuring the amount of nitrous oxide (N2O) discharged by plants. The particular measuring process is that: fresh plant branches and leaves are taken, washed, fragmented and put in a homogenate cup; right-amounted precooling homogenate buffer solution is put in the cup and homogenated. The homogenated solution is put in a non-light tight container and the container is sealed and put under light to be cultured. GC-ECD is used for measuring the volume concentration of the nitrous oxide (N2O). According to the increase of the quality of the nitrous oxide (N2O) in the container gas phase, the volume of the gas phase in the container and the dry weight of plants during the time of culturing, the discharge flux of the nitrous oxide (N2O) of the plants is calculated. The invention solves the problems that the prior enclosed box method is time-consuming, easy to injure plants and can not be used to measure the plants with big size or small discharge amount. The invention is a method of measuring the amount of nitrous oxide(N2O) discharged by plants, is applicable to the plants with different sizes and different discharge amounts and is of simple operation and low cost.