The invention discloses a stratified sampling device of surface water. The stratified sampling device of the surface water mainly comprises a sampling inner rod, a sampling bottle fastener, a sampling bottle, a sampling outer rod, a sampling bottle plug, a sampling bottle plug fastener, a clamping buckle 1, a clamping buckle 2, a sampling outer rod penetrating hole, a sampling inner rod penetrating hole, a clamping buckle hole 1, a clamping buckle hole 2, a clamping buckle 3, a sampling bottle plug rod and a sampling bottle rod. Sampling is conducted in the vertical direction of a water body, by means of a common stratified sampling device of the surface water, water samples usually are sampled in the vertical direction by several times, when the water body is sampled by several times, the disturbance to the water body is relatively large, and it cannot be ensured that sampling is conducted completely at the same point, the authenticity of the measured data is affected to a certain extent, and meanwhile, and fractional sampling increases the sampling workload. Therefore, the stratified sampling device of the surface water is designed and studied, and the device is simple in structure and convenient in operation, meanwhile, multiple groups of required water samples can be obtained in the vertical direction at the sampling point, and the disturbance to the water body is reduced.