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Method for determining dinitrogen monoxide discharging quantity of plants

A nitrous oxide and emission technology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of easily damaged plants, cannot be used to measure plants with tall individuals or plants with small emissions, and is time-consuming, and achieves low cost. , Simple operation, wide application effect

Inactive Publication Date: 2008-04-16
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for measuring the nitrous oxide emission of plants, which solves the problems of time-consuming, easy to damage plants, and cannot be used to measure plants with small individual height or low emission in the commonly used closed box method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Weigh 5 parts of fresh spinach, 20 grams each, wash it with tap water-distilled water-sterile water, and absorb the surface moisture with filter paper.

[0027] 2. After cutting the above samples into small sections, put them into pre-cooled homogenization cups respectively, and then add pre-cooled 30mL HEPES homogenization buffer (weight of spinach (grams): volume of HEPES homogenization buffer (ml)=1: 1.5), the homogenization cup and the homogenization buffer are pre-cooled in the refrigerator (about 4°C) overnight until the constant temperature is reached.

[0028] 3. Homogenize for 30 seconds.

[0029] 4. Put the homogenate into 300mL drip bottles respectively, and after sealing with anti-stoppers, immediately take a zero-time gas sample from the bottles with a syringe.

[0030] 5. Place the glass vial at a light intensity of 100μmol·m -2 ·s -1 Under the condition of incubation for 2 hours, the final gas sample was taken from the bottle.

[0031] 6. Determina...

Embodiment 2

[0034] 1. Weigh 5 parts of fresh celery, 30 grams each, wash and absorb the surface moisture.

[0035] 2. After cutting the above samples into small pieces, put them into pre-cooled homogenization cups respectively, and then add 60mL pre-cooled HEPES homogenization buffer. Pre-cool overnight until constant temperature is reached.

[0036] 3. Homogenize for 40 seconds.

[0037] 4. Put the homogenate into 500mL drip bottles respectively, and seal it with a stopper, and immediately take a zero-time gas sample from the bottle with a syringe.

[0038] 5. Place the glass bottle at 90μmol·m -2 ·s -1 Incubate under light intensity conditions for 3 hours, and take a final gas sample from the bottle.

[0039] 6. Determination of N in gas samples by GC-ECD 2 O volume concentration.

[0040] 7. According to the gas phase volume in the culture bottle, the dry weight of the plant material to be tested and the N in the culture bottle during the culture time 2 The increment of O quality,...

Embodiment 3

[0042] 1. Collect 20 soybean plants in the early flowering stage from the field, and cultivate them in the laboratory nitrogen-free culture solution overnight until the constant temperature is reached.

[0043] 2. Take the aboveground part of the soybean plant, wash and dry it.

[0044] 3. Cut off the leaves, petioles and stems, weigh them respectively, and calculate the weight ratio of leaves to petioles and leaves to stems.

[0045] 4. Immediately take 5 parts of leaves, 100 grams each, and weigh the petiole and stem of the corresponding weight according to the ratio calculated in 3, and cut them into small sections.

[0046] 5. Cut the leaves into pieces and put them into pre-cooled homogenization cups, add 400mL pre-cooled HEPES homogenization buffer, and pre-cool the homogenization cup and homogenization buffer in the refrigerator (about 4°C) overnight. until a constant temperature is reached.

[0047] 6. Homogenize for 1 minute.

[0048] 7. Put the homogenate and corr...

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Abstract

The invention belongs to the global change- terrestrial ecosystem-greenhouse gas change field, in particular to a method of measuring the amount of nitrous oxide (N2O) discharged by plants. The particular measuring process is that: fresh plant branches and leaves are taken, washed, fragmented and put in a homogenate cup; right-amounted precooling homogenate buffer solution is put in the cup and homogenated. The homogenated solution is put in a non-light tight container and the container is sealed and put under light to be cultured. GC-ECD is used for measuring the volume concentration of the nitrous oxide (N2O). According to the increase of the quality of the nitrous oxide (N2O) in the container gas phase, the volume of the gas phase in the container and the dry weight of plants during the time of culturing, the discharge flux of the nitrous oxide (N2O) of the plants is calculated. The invention solves the problems that the prior enclosed box method is time-consuming, easy to injure plants and can not be used to measure the plants with big size or small discharge amount. The invention is a method of measuring the amount of nitrous oxide(N2O) discharged by plants, is applicable to the plants with different sizes and different discharge amounts and is of simple operation and low cost.

Description

technical field [0001] The invention belongs to the field of global change science-terrestrial ecosystem-atmosphere greenhouse gas exchange, and is specifically a method for measuring plant nitrous oxide (N 2 O) method of emissions. Background technique [0002] According to the report (2007) of the Intergovernmental Panel on Climate Change (IPCC) affiliated to the United Nations, global warming is likely to be caused by the increase of greenhouse gas emissions caused by the enhancement of human activities. Nitrous oxide (N 2 O) is an important greenhouse gas, and its warming potential is about 2 300 times and CH 4 29 times. N 2 O is a greenhouse gas of biological origin, and 70-90% of its total emissions into the atmosphere come from biological emissions. In the past, it was believed that the nitrification-denitrification of microorganisms is the production of N 2 O's only biological process. In 1989, Chinese scholars were the first to report that plants themselves ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/00G01N33/00
Inventor 陈冠雄倪志龙周丽沙张颖李慧韩斯琴史荣久杨伟超丁雷徐慧
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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