65 results about "Stable nuclide" patented technology

The invention discloses a water pollution cross-domain traceability method based on multi-chain collaboration and stable isotope source analysis, and relates to the technical field of water environment monitoring, the method comprises the following steps: pollution source chemical fingerprint construction: collecting an environmental water sample and a potential pollution source sample in a drainage basin; adopting a mass spectrometer to detect stable isotope compositions such as delta 13C, delta 15N, delta 18O and the like in the pollutants; and performing morphological division on the pollutants by using a morphological grading extraction technology. Homology judgment precision is greatly improved through isotope and morphological analysis; cross-chain credible collaboration is achieved, multi-party data interaction is achieved, and timeliness and accuracy of pollution tracing are guaranteed; the space-time model traceability is combined with an evolution path and gridding mapping, so that the pollution inversion accuracy is improved; and ecological compensation support provides a quantitative basis for pollution intensity and diffusion range so as to support a compensation model.

The present invention provides a pretreatment method for determining the stable isotope ratio of non-exchangeable hydrogen in acetic acid, belonging to the field of stable isotope analysis of foods. The pretreatment method comprises the following steps: adding a reaction reagent to a vinegar sample extract to replace the carboxyl hydrogen in the acetic acid with the exchangeable hydrogen to achieve isotope exchange equilibrium. The present invention can be used to simply and rapidly determine the isotope ratio of non-exchangeable hydrogen in acetic acid in vinegar products and can be used in research and applications for detecting the authenticity or adulteration of vinegar products.

The invention discloses a method for determining carbon isotopes of low-concentration soluble organic carbon in a water body, and belongs to the technical field of organic carbon detection. The determination method comprises the following steps: combining a TOC analyzer and a stable isotope mass spectrometer, and converting DOC in a water body into CO2 gas by adopting the TOC analyzer; cO2 gas generated by the TOC analyzer is enriched for the first time through the cold trap T1; the CO2 gas frozen by the cold trap T1 is enriched for the second time through a cold trap T2; introducing the CO2 subjected to secondary enrichment into a stable isotope mass spectrometer, and determining the carbon isotope composition of the CO2. Aiming at a low-concentration DOC water body, the carbon isotope composition of DOC in the water body is determined on the basis of combination of a wet oxidation technology and stable isotope mass spectrometry detection, a wet oxidation method is used for increasing the volume of a water sample to generate more CO2 for stable isotope mass spectrometry determination, so that more accurate carbon isotope composition is obtained, and the method is efficient, time-saving, labor-saving and accurate.

The invention discloses a mine water source identification method, system, equipment and medium based on a combined weight and grey relational degree theory, and relates to the technical field of mine water source identification, and the method comprises the steps: obtaining water quality parameters and stable isotope composition of a mine water sample, and building a comprehensive feature matrix; calculating the correlation degree between each water quality parameter and a preset water source type based on the comprehensive feature matrix, analyzing the composition and proportion of multi-source water mixing in the mine water sample, and determining a first mine water source identification result; and weighting the water quality parameters and the stable isotope composition in the comprehensive characteristic matrix by using a combined weight method, and obtaining a second mine water source identification result based on the weighted comprehensive characteristic matrix and the first mine water source identification result. The method has high prediction speed and prediction precision, the existence condition of the underground water source can be identified by using a geophysical prospecting method according to different geological conditions through the prediction model, and the acquisition process of the underground water burst source is more convenient.

The utility model relates to the technical field of gas transfer, and provides a sample introduction device for gas stable isotope value measurement, which comprises a containing tank with a containing space and a plurality of communicating pipes communicated with the containing space; the high-concentration gas injection pipe is arranged on the containing tank and communicated with the containing space, and high-concentration detection gas can enter the containing space through the high-concentration gas injection pipe. By means of the technical scheme, the technical problems that when a gas pre-concentration device is used in the prior art, gas cannot be directly supplied for high-concentration gas, the high-concentration gas can only be detected after being diluted in advance, the detection cost is increased, and the detection result has errors are solved.

The invention relates to the technical field of metallurgy, and discloses an intelligent steelmaking process based on dynamic raw material proportioning, the core of the process is that a functional pellet with a core-shell-film multilayer structure is adopted, functional components sequentially acting in a molten pool are packaged in different layers of the functional pellet respectively, and the functional components are sequentially separated from the molten pool. The stable isotope tracers are in one-to-one correspondence with the functional components and can be released in sequence. A mass spectrometer sensing system is used for detecting specific time sequence signals formed by sequential release of the tracer agents on line, and the system can confirm the delivery and step-by-step reaction process of materials and conduct on-line self-correction on kinetic parameters in a metallurgical process model. According to the method, a closed-loop control system integrating precise feeding, process confirmation and model adaptive capacity is constructed, and adaptive control over the steelmaking process is achieved. The method can improve the control precision of molten steel components and temperature at the smelting end point, shorten the smelting period, and improve the overall production efficiency and the resource utilization rate.

The present application relates to a kind of detection methods of isotopic abundance in acetic acid, comprising the following steps: stable isotope labeled acetic acid is decomposed under vacuum and high temperature conditions, generates stable isotope labeled methane or carbon dioxide, can determine 13 C labeled in carboxyl site acetic acid CH3 13 COOH in 13 C isotopic abundance, 13 C labeled in methyl site acetic acid 13 CH3COOH in 13 C isotopic abundance, 18 O labeled acetic acid CH3C 18 O 18 OH in 18 O isotopic abundance, methyl deuterium isotopic abundance in deuterium labeled methyl site acetic acid CD3COOH;Compared with prior art, the present application has the advantages that the isotopic abundance in acetic acid labeled by stable isotope in different sites is detected, can be used for deuterium, 13 C or 18 O labeled acetic acid isotopic abundance determination, test data accuracy and precision etc.

The invention provides a medicine raw material homology judgment method and device, computer equipment and a medium, and belongs to the field of medicine identification, and the method comprises the following steps: obtaining a plurality of same sample medicines from different sources; determining the stable isotope abundance values of carbon, nitrogen, oxygen and hydrogen in the drug sample through a stable isotope ratio mass spectrometry and a chromium-assisted stable isotope ratio mass spectrometry; taking the stable isotope abundance value as a variable, and classifying sample drug sources by adopting a hierarchical clustering analysis and principal component analysis method to obtain a plurality of drug sources; obtaining stable isotope abundance values of to-be-classified target drugs, and respectively determining the stable isotope abundance values of the target drugs and Z values of the stable isotope abundance values of all classes based on a Z value analysis method; under the condition that the Z values of the stable isotope abundance values of all the elements are smaller than or equal to the preset threshold value, it is determined that the target drug and the sample drug of the corresponding category are homologous drugs, and therefore the accuracy of drug detection is improved.

The embodiment of the application provides a marine carbonate high-resolution sequence stratigraphic division method and device, and belongs to the technical field of geological exploration. The method comprises the following steps: obtaining logging data of a target well section, generating a first curve based on the logging data, the first curve being a curve for representing the rise and fall of the paleo-sea level of the target well section; obtaining stable isotope data of the target well section, correcting the first curve based on the stable isotope data, obtaining a second curve, and thus establishing a high-resolution sequence division scheme of a single well; obtaining a seismic profile of the target well section, determining a negative inflection point of the second curve, and calibrating the seismic profile based on different levels of the negative inflection point of the second curve, so as to determine a high-resolution sequence tracking scheme of the target well section; and the application can quickly and conveniently solve the problem of high-resolution sequence stratigraphic division and comparison.

The application relates to the technical field of plasma, in particular to a medical cyclotron beam centering adjustment method and system. The method comprises the following steps: acquiring a magnetic field sequence, a beam sequence and a current sequence; obtaining a beam fluctuation factor according to the difference in lag number corresponding to the maximum autocorrelation coefficient of the magnetic field sequence and the beam sequence, the correlation of the magnetic field sequence and the beam sequence, and the difference in the maximum autocorrelation coefficient; forming a beam fluctuation sequence by using the beam fluctuation factor; dividing the beam fluctuation sequence, and also dividing the current sequence; fitting a straight line based on the current fluctuation sequence and the current sequence; obtaining the current influence intensity at the current moment according to the difference between the excitation current in all sub-sequences and the overall excitation current of the current sequence and the mean square error; improving the proportional term according to the current influence intensity, controlling the excitation current based on the proportional term, and adjusting the proportional term in real time to optimize the magnetic field control effect. The application realizes higher and more stable nuclide production efficiency.

The invention discloses a method for high-precision detection of carbon stable isotope and content thereof in an environmental sample. The method provided by the invention comprises the following steps: detecting environmental sample powder by using an elemental analysis-stable isotope mass spectrometer; the sampling amount of the environmental sample powder is greater than or equal to 0.4 mg; in the detection process, inert gas is introduced into an opening flow divider in a Conflo universal interface of the elemental analysis-stable isotope mass spectrometer, and the inert gas is mixed with sample gas obtained by combustion in an elemental analyzer. According to the method provided by the invention, the situation that a sample signal is too high and exceeds a linear range is avoided, the representativeness of the sample is improved, the possibility of data abnormity caused by nonuniformity of the sample is reduced, and the aim of rapidly and accurately detecting the stable carbon isotope and the content of the stable carbon isotope at the same time is fulfilled.

The present invention proposes a method for determining the stable isotope ratio of non-exchangeable hydrogen sites of glycerol in edible oils and fats, belonging to the field of stable isotope analysis technology. The method comprises the following steps: a) preparing a preliminary glycerol sample; b) determining the stable hydrogen isotope ratio of glycerol in the preliminary glycerol sample using a gas chromatography-pyrolysis-stable isotope ratio mass spectrometer; c) selecting glycerol with a known stable isotope ratio of non-exchangeable hydrogen sites as a working standard substance, processing according to step a), and determining according to step b) to obtain the stable hydrogen isotope ratio of the glycerol working standard substance; and calculating the stable isotope ratio of the non-exchangeable hydrogen sites of glycerol in the edible oil to be tested by comparing the measurement result of the stable hydrogen isotope ratio of the glycerol working standard substance with the known stable isotope ratio of the non-exchangeable hydrogen sites of the glycerol working standard substance. The method is rapid and accurate, providing a new analytical method for adulteration detection and authenticity control of oils and fats.

The present invention relates to a 13 C. 18 The synthesis method of trifluoroacetic acid with O stable isotope double labeling is as follows: firstly, trifluoroacetic acid is dissolved in a solvent, metal ions are added, and 13 C-labeled carbon dioxide, react to obtain a reaction solution, then separate and dry the reaction solution to obtain 13 C-enriched trifluoroacetic acid, and finally 13 C-enriched trifluoroacetic acid is dissolved in an aprotic solvent, thionyl chloride is added, stirred for reaction, and then a catalyst and oxygen 18 water are added, heated for reaction, and the reaction product is separated to obtain 13 C. 18 O stable isotope double labeled trifluoroacetic acid is the target product. Compared with the prior art, the present invention has a simple reaction process, a simple post-processing process, and a high yield. 13 The C isotope abundance is over 98%. 18 The O isotope abundance is over 97%.

The invention relates to the technical field of detection, and discloses a method for tracing the origin of silkworm cocoons based on a stable isotope technology in combination with a machine learning algorithm, and the method comprises the steps: (1) collecting data; (2) data preprocessing; (3) model optimization; (4) model training; and (5) producing area tracing. According to the invention, origin traceability of silkworm cocoons from different origins is realized based on isotope analysis and a machine learning algorithm. Specifically, hydrogen, oxygen, carbon and nitrogen stable isotope ratio data are combined with KNN, SVM and RF algorithms to realize rapid and high-precision source tracing of silkworm cocoon producing areas. According to the invention, by combining the advantages of various machine learning algorithms, the accuracy and reliability of silkworm cocoon origin tracing are improved.

The invention provides an experimental method for determining stable carbon and oxygen isotopes by a two-way sampling method, and relates to the technical field of stable isotope analysis. The experimental method comprises the following steps: selecting carbon dioxide gas with the purity of more than 99.999% as carbon dioxide reference gas, and selecting a calcium carbonate carbon and oxygen isotope standard substance and a target sample to be measured; and then the stable carbon and oxygen isotopes are determined through a two-way sample introduction method, standard gas does not need to be purchased, the isotope ratio of a sample relative to a standard substance is obtained through conversion application of a formula, finally, the isotope ratio is successfully calibrated to the value of international standard PDB, and the experiment cost is saved. The method can be widely applied to other stable isotope two-way sample introduction methods, principle communication is achieved, and determination can be conducted through the experimental method under the condition that no standard gas exists.

The invention relates to a pollution monitoring method and device based on stable isotopes, computer equipment and a storage medium. The method comprises the following steps: acquiring a volatilization fractionation parameter, a dissolution fractionation parameter and an adsorption fractionation parameter of a target pollutant and sample fractionation data corresponding to the target pollutant; constructing an initial evaluation model according to the volatilization fractionation parameters, the dissolution fractionation parameters and the adsorption fractionation parameters; training the initial evaluation model according to the sample fractionation data, and determining a target evaluation model; acquiring local fractionation data of the target pollutant; evaluating local fractionation data according to the target evaluation model, and determining fractionation data of a second target phase state of a target pollutant in a target environment; and determining the mass flux of the target pollutant in the target environment according to the local fractionation data and the fractionation data of the second target phase state, thereby efficiently monitoring the high-density non-aqueous phase liquid pollutant. The method can be adopted.

The invention discloses a coal spontaneous combustion index gas traceability and analysis method based on isotope fingerprints, which comprises the following steps: drilling and sampling each coal seam of a coal mine to prepare desorbed gas, and measuring the abundance ratio of gas isotope to form an isotope fingerprint characteristic spectrum; drawing stable carbon-hydrogen-oxygen isotope fingerprint characteristic spectrums of the coal seam coal sample oxidation process at different temperatures through a temperature programming-gas chromatography-stable isotope mass spectrometer combined system; the method comprises the following steps: collecting underground non-spontaneous combustion area gas, measuring a normal background isotope value, and establishing a background database and stable isotope fingerprint characteristics of various gases to jointly construct a coal mine carbon-hydrogen-oxygen isotope fingerprint database; and applying a physical constraint optimization sub-source quantification mathematical model, and combining a Bayesian depth inversion method to realize rapid matching and high-precision traceability of stable isotope measured data and a fingerprint database. According to the method, tracing analysis of coal spontaneous combustion index gas abnormity in the shallow-buried short-distance multi-coal-seam mining process can be achieved, the coal spontaneous combustion disaster site is positioned, and safe production of a coal mine is guaranteed.

The invention relates to the technical field of plasmas, in particular to a medical cyclotron beam centering adjustment method and system. The method comprises the following steps: acquiring a magnetic field sequence, a beam sequence and a current sequence; acquiring a beam oscillation factor according to the lag number difference corresponding to the maximum autocorrelation coefficient of the magnetic field sequence and the beam sequence, the correlation of the magnetic field sequence and the beam sequence and the difference of the maximum autocorrelation coefficient; forming a beam oscillation sequence by the beam oscillation factors, dividing the current sequence after dividing the beam oscillation sequence, fitting a straight line based on the current oscillation sequence and the current sequence, and obtaining the current influence intensity at the current moment according to the difference between the excitation current in all subsequences and the overall excitation current of the current sequence and the mean square error; the proportional term is improved according to the current influence intensity, the excitation current is controlled based on the improvement, and the proportional term is adjusted in real time to optimize the magnetic field control effect. According to the invention, high-efficiency and stable nuclide production efficiency is realized.

The invention discloses a method for monitoring carbon leakage in a carbon dioxide mineralization process by using stable isotopic tracing. The method comprises the following steps: measuring a delta 13C value of a CO2 gas source used in the carbon dioxide mineralization process; a CO2 gas source is injected into the carbon dioxide mineralization process, and then the carbon dioxide mineralization process flow is started; collecting each key point sample in the carbon dioxide mineralization process; measuring and analyzing the delta 13C value of the sample at each key point; and calculating the carbon leakage rate according to the measured delta 13C value of the sample at each key point, and evaluating the mineralization effect. According to the method, CO2 migration and leakage risks are tracked in real time by injecting CO2 with a specific carbon stable isotope ratio (delta13C) and combining on-site laser spectrum analysis and multi-isotope detection, the monitoring precision and the quantification capacity are improved, the method is suitable for a carbon dioxide mineralization utilization project, and the environmental risk is reduced.

The invention discloses a preparation method of a tri-silicon isotope standard sample for a celestial body sample, and belongs to the field of geochemical stable isotopes. The method comprises the following steps: preparing two basic materials according to the average composition of the most representative silicate rock on the earth, decarbonizing, grinding and uniformly mixing; adding a silicon-29 diluent into one part of the basic material, and repeatedly sintering and grinding to obtain a material A; mixing a part of the material A with another part of the basic material, and sintering and grinding again to obtain a material B; the material B is subjected to sample dissolving and chemical purification to obtain a trisilicon isotope standard sample, MC-ICP-MS testing and silicon isotope abnormity calculation are carried out on the standard sample, it is found that the prepared standard sample is uniform in component and clear in non-mass-related fractionation signal, the standard sample can be used for correcting the mass discrimination effect in the analysis process of the sample to be detected, and the quality of the sample to be detected is improved. The silicon isotope abnormity in the celestial body sample is accurately identified, the accuracy and comparability of an analysis result are improved, and a reliable experimental reference is provided for planetary science and geochemical research.

The invention belongs to the technical field of ecological environment monitoring and tracing, and particularly relates to a method for evaluating the contribution rate of exogenous carbon in a mangrove forest. According to the method, two technologies of stable isotope analysis and eDNA analysis are combined, and the limitation of a single method in tracing the carbon source of the mangrove forest is overcome. The stable isotope analysis can evaluate the relative contribution of different sources from the perspectives of elemental composition and isotope ratio, the environmental DNA can provide high-resolution species information and determine the contribution of specific plant species or class groups to carbon, the two complement each other, the contribution rate of mangrove forest exogenous carbon is quantitatively evaluated, the accuracy of carbon source tracing is greatly improved, and the carbon source tracing efficiency is improved. The technical problem of carbon source analysis in a complex environment with multi-source carbon input is solved.

The invention discloses a corrosion product deposition test method based on stable isotope tracing, which comprises the following steps: in a corrosion product out-of-pile deposition simulation test, actively adding a low-abundance isotope corrosion product into a loop, migrating the low-abundance isotope corrosion product in the loop and depositing the low-abundance isotope corrosion product on the surface of an alloy test piece, distribution of isotopes with different depths on the surface of the alloy test piece is analyzed after the test is carried out, and the oxidation film layer and the corrosion product deposition layer on the surface of the alloy test piece are distinguished. The invention provides a corrosion product deposition test method based on stable isotopic tracing, which comprises the following steps: artificially adding a low-abundance isotope corrosion product of an alloy into a test loop, so that the low-abundance isotope corrosion product migrates in the loop and is deposited on the surface of an iron-based alloy or a nickel-based alloy to form a corrosion product deposition layer; and distinguishing and identifying the oxidation film layer and the corrosion product deposition layer of the iron-based alloy and the nickel-based alloy in a stable isotope tracing manner.

The application discloses a method suitable for judging fluid migration direction of hydrothermal deposit, selects hydrothermal deposits with correlation in a study area; collects hydrothermal mineral samples from each hydrothermal deposit, determines and marks corresponding metallogenic stages of each hydrothermal deposit, divides the hydrothermal deposit into pre-mineralization stage, mineralization stage and post-mineralization stage based on the metallogenic stages; respectively tests stable isotopes of the hydrothermal mineral samples in the pre-mineralization stage, the mineralization stage and the post-mineralization stage, judges mineral stable isotope variation rules corresponding to the hydrothermal mineral samples in different mineralization stages; compares and studies the mineral stable isotope variation rules of the hydrothermal mineral samples in each mineralization stage of two or more hydrothermal deposits, regards a direction in which the mineral stable isotope variation rules of the hydrothermal deposits tend to be consistent as a fluid migration direction; selects another isotope for verification; the application regards the stable isotope variation rule as an effective means for tracing the source of the hydrothermal fluid of the deposit.

The present invention belongs to the technical field of mass spectrometers, and particularly relates to an ion source for a stable isotope mass spectrometer. Its technical solution is as follows: an ion source for a stable isotope mass spectrometer comprises a fixed base, one side of the fixed base is connected to a high-voltage electrode, the other side of the fixed base is provided with a plurality of support columns, the other side of the support columns is connected to a limit seat, an ionization chamber for bombarding a sample, a correction unit for correcting the ion beam, and a slit unit for adjusting the width of the ion beam are sequentially installed between the support columns, an acceleration unit for adjusting the ion beam speed is connected to the limit seat, the fixed base is connected to a cavity, the cavity is sleeved outside the plurality of support columns, the cavity is connected to an injection valve for inputting a sample into the ionization chamber, and the ionization chamber, correction unit, slit unit, and acceleration unit are all connected to the high-voltage electrode via a high-voltage wire. The present invention provides an ion source for a stable isotope mass spectrometer that is convenient for adjusting the width and energy of the ion beam.

The invention discloses a stable isotope sample treatment workstation and a sample treatment method thereof, the workstation comprises a main frame, a liquid adding system and a gland system, the liquid adding system and the gland system are arranged in the main frame, the main frame comprises a workbench and a supporting frame on the upper side of the workbench, and the workbench is provided with a placing frame and an activating frame; the liquid adding device comprises a first transverse moving frame and a needle frame arranged on the first transverse moving frame in a liftable mode, a liquid adding needle is arranged on the lower side of the needle frame, the liquid sucking and discharging device is communicated with a liquid adding needle pipe, and the liquid adding device is matched with the liquid sucking and discharging device to work, so that a plurality of reaction liquid agents can be injected into the reaction bottles in sequence; the capping system comprises a second transverse moving frame, a cap falling device and a clamping device, the cap falling device and the clamping device are arranged on the second transverse moving frame, the second transverse moving frame transversely slides to drive the cap falling device to pass through the position above a reaction bottle, an aluminum cap can be hooked out by the reaction bottle and buckled to a bottle opening of the reaction bottle, and then the aluminum cap on the reaction bottle can be clamped through work of the clamping device. The subsequent reaction work can be smoothly carried out.

The invention discloses a device and a method for testing dissolved oxygen and ozone isotopes in water, and belongs to the field of gas stable isotope analysis and testing. Comprising a constant-pressure constant-current automatic sampling device and a gas sampling system, a certain volume of water sample in a sealed sample bottle is removed by using the constant-pressure constant-flow automatic sampling device for dissolved oxygen in water, the water sample is placed in the constant-temperature balance oscillation device for balancing, back flushing of a gas circuit is realized by using the gas sampling system, the influence of residual gas is eliminated, and then the sample is collected and purified. Purified gas passes through a multi-channel gas path switching system, separated oxygen is loaded into a gas stable isotope mass spectrometer, oxygen isotopes of dissolved oxygen are detected, and therefore automatic pretreatment of the dissolved oxygen in water and accurate testing of the three oxygen isotopes of the dissolved oxygen are achieved. According to the method, full-automatic operation is realized, man-made interference and air pollution are effectively avoided, and the detection precision and efficiency are remarkably improved.

The invention provides a multi-element monomer stable isotope source analysis method for chlorinated hydrocarbon in underground water, and relates to the field of underground water pollution traceability, the method comprises the following steps: constructing a three-dimensional isotope characteristic matrix according to a determined target pollutant and a corresponding carbon, chlorine and hydrogen monomer stable isotope system; the method comprises the following steps: arranging sampling points in a polluted site, collecting an underground water sample and pre-treating; performing isotope determination on the sample to obtain isotope data; constructing a fractionation model of carbon, chlorine and hydrogen isotopes; inputting the isotope data into a fractionation model for correction; and performing clustering analysis in a three-dimensional isotope space by using the corrected isotope data in combination with the three-dimensional isotope feature matrix, identifying different pollution sources, and performing result verification in combination with chemical fingerprint features and an underground water flow field model. According to the technical scheme, dependence on traditional concentration discrimination is weakened, and fine discrimination and contribution quantification between different pollution sources are achieved.

This invention discloses an online observation and calibration method for stable isotopes of atmospheric water vapor using laser spectroscopy. The method includes: acquiring standard water vapor and atmospheric water vapor stable isotope data; performing memory effect calibration by selecting a specific time period and limiting the standard deviation range to eliminate residual errors; calculating the minute drift between two consecutive concentrated standard sample tests to perform drift effect calibration over time; establishing a nonlinear fitting relationship between the difference in standard water vapor concentration and the difference in stable isotopes to perform concentration effect calibration; and finally, establishing a linear mapping relationship between the instrument's average test value at the reference concentration and the true value of the standard sample to complete the true value standardization. This invention systematically eliminates the comprehensive systematic errors such as memory, drift, and concentration generated in online laser spectrometer observations, ensuring the long-term accuracy and reliability of the observation data.

The invention discloses a method for measuring an element distribution coefficient and an isotope fractionation value between a fluid and a melt, and belongs to the field of stable isotope geochemistry, and the method comprises the following steps: preparing an initial fluid and an initial solid phase; performing isotope exchange reaction by using the initial fluid and the initial solid phase; an experimental fluid phase and an experimental melt phase in a reaction product are separated through freezing and deionized water cleaning; and determining the metal element content and stable isotope composition of the experimental fluid phase and the experimental melt phase, and calculating the metal element distribution coefficient and isotope fractionation value between the experimental fluid phase and the experimental melt phase. According to the method, after isotope exchange equilibrium of the rapid quenching cold-sealed autoclave, the fluid phase and the silicate rock phase are frozen and then separated, the fluid phase and the silicate rock phase are precisely separated and recovered through multi-step deionized water cleaning, precise analysis of the metal element content and the isotope composition of the two phases is achieved, and the analysis precision of the metal element content and the isotope composition of the two phases is improved. And the method has positive significance on isotope geochemistry development.