Method for judging place of production of traditional Chinese medicinal pulsatilla by using stable isotope ratio

A stable isotope, Pulsatilla spp. technology, applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve problems such as differences in the content of active ingredients, complex origins of traditional Chinese medicines, and few applications of stable isotope mass spectrometry technology, achieving low cost. , The effect of solving the origin traceability and safeguarding the interests of consumers

Inactive Publication Date: 2014-03-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the stable isotope mass spectrometry technology is rarely used in the identification of the origin of traditional Chinese medicine; moreover, the origin of traditional Chinese medicine is more complicated. A Chinese medicinal material often has several or even dozens of origins. The content is also different
In view of the particularity of the identification of the origin of traditional Chinese medicine, there is currently no specific method for identifying the origin of Pulsatilla pulsatillae by using stable isotope mass spectrometry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1.1 N%, C%, δ of Pulsatilla samples from the real place of origin 15 N and δ 13 C determination

[0025] 1) Real Pulsatilla samples were excavated from Heilongjiang, Liaoning, Anhui, Henan, Gansu and other places where Pulsatilla is mainly produced;

[0026] 2) Take the sample rhizome of Pulsatilla pulsatillae, air-dry and preserve;

[0027] 3) Before the determination of stable isotope mass spectrometry, take a small amount of sample and heat it in an oven at 60°C for 1 hour, then pulverize it after cooling, pass it through a 60-mesh sieve, and wait for the test;

[0028] 4) Wrap the sample in a tin foil boat, and use elemental analysis-stable isotope mass spectrometry for δ 15 N, δ 13 C, N%, C% are measured; The measurement results are as shown in Table 1:

[0029] Table 1

[0030] Numbering

[0031] It can be seen from Table 1 that the δ 15 N and δ 13 The difference in C value is significant; while the Pulsatilla 15 N and δ 13 C values ​​are rela...

Embodiment 2

[0033] 2.1 Construction of the δ15N and δ13C databases of Pulsatilla radiata from the real place of origin

[0034] 1) Take Psyllium pulsatilla samples from different known origins;

[0035] 2) Take the sample rhizome of Pulsatilla pulsatillae, air-dry and preserve;

[0036] 3) Before the determination of stable isotope mass spectrometry, take a small amount of sample and heat it in an oven at 60°C for 1 hour, after pulverization, pass through a 60-mesh sieve, and wait for the test;

[0037] 4) Wrap the sample in a tin foil boat, and use elemental analysis-stable isotope mass spectrometry for δ 15 N, δ 13 Determination of C, N%, and C%, to construct the true origin of Pulsatilla δ 15N and δ 13 C database; the database is shown in Table 2.

[0038] Table 2

[0039] Numbering

[0040] 2.2 Origin traceability of Pulsatilla grandis

[0041] 1) Purchase samples of Pulsatilla pulsatilla medicinal materials from the market;

[0042] 2) Before the determination of s...

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Abstract

The invention discloses a method for judging the place of production of traditional Chinese medicinal pulsatilla by using a stable isotope ratio. According to the method, the values of N%, C%, sigma 15N and sigma 13C in a pulsatilla sample are measured by an elemental analysis-stable isotope mass spectrometry technology. The method mainly comprises the following steps of constructing a database including N%, C%, sigma 15N and sigma 13C of the pulsatilla sample in the real place of production by measuring the values of N%, C%, sigma 15N and sigma 13C in the pulsatilla samples in different places of production; measuring the values of N%, C%, sigma 15N and sigma 13C in an unknown place of production; performing Fisher judgment analysis by statistical software, and running a program to judge the real place of production of the pulsatilla sample in the unknown place of production. According to the method disclosed by the invention, the method for accurately judging the place of production of the pulsatilla can be constructed by the elemental analysis-stable isotope mass spectrometry technology, so that the technical difficulty in tracing of the place of production of traditional Chinese medicines in China can be solved; the benefit of a customer can be protected, and the traditional Chinese medicine industry can be developed in a healthy and ordered way.

Description

technical field [0001] The invention belongs to the technical field of traceability of the origin of the traditional Chinese medicine Pulsatilla pulsatillae, and specifically relates to a method for determining the origin of the traditional Chinese medicine Pulsatilla pulsatillae by using the ratio of stable isotopes. Background technique [0002] Pulsatilla is a common species of Chinese medicinal materials with high medicinal value. It has the effects of clearing away heat and detoxification, cooling blood, improving eyesight, and stopping dysentery. , carbuncle sore, scrofula. Distributed in Liaoning, Hebei, Jilin, Henan, Anhui, Gansu and other places, the ones produced in Anhui were used as authentic medicinal materials in ancient times. Due to different natural conditions and ecological environments, the quality of medicinal materials varies from place to place. Authentic medicinal materials are better in quality and curative effect than those produced in other regions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 张莉陈剑梁莉莉梁齐
Owner SHANGHAI JIAO TONG UNIV
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