95 results about "Pulsatillae" patented technology

The invention discloses pulsatilla saponin B4-oleanolic acid self-assembled nanoparticles as well as preparation and application thereof, and relates to the technical field of pharmaceutical preparations. Inspired by compatibility and solubilization of traditional Chinese medicines, the pulsatilla saponin B4-oleanolic acid self-assembled nanoparticles (AB4-OA NPs) are prepared by taking natural active components, namely micromolecular pulsatilla saponin B4 and oleanolic acid, as raw materials; the oleanolic acid has the effects of promoting dissolution and resisting inflammation, the water solubility of the oleanolic acid is improved, the bioavailability is improved, and the anti-inflammatory effect of the oleanolic acid is enhanced. Experimental results show that the AB4-OA NPs can effectively improve the dissolution rate of the oleanolic acid, so that the bioavailability of the oleanolic acid is effectively improved, meanwhile, the AB4-OA NPs has a better anti-inflammatory effect, and the effect is better than that of a single drug with the same dosage. In addition, the synthetic preparation method is simple and convenient, has the advantages of nano-scale drugs, realizes auxiliary-material-free pure drug self-delivery, and improves the delivery efficiency.

The invention relates to Chinese pulsatilla root and stem exosome-like vesicles as well as a preparation method and application thereof. The preparation method comprises the following steps: juicing Chinese pulsatilla roots, filtering, and collecting filtrate; and centrifuging the filtrate, collecting the precipitate, re-suspending, and filtering to obtain a solution containing the exosome-like vesicles of the Chinese pulsatilla roots. Compared with the prior art, the Chinese pulsatilla root and stem exosome-like vesicles extracted by the method can inhibit malignant progression, including proliferation and migration, of lung cancer, and cell uptake and animal living imaging prove that the Chinese pulsatilla root and stem exosome-like vesicles can be taken by cells and can be enriched in tumor tissues at the same time.

Disclosed are a Pulsatilla saponin B4 derivative, a preparation method therefor, and a use thereof. A compound Pulsatilla saponin B4 (referred to as B4 or AB4) is used as a raw material, a nucleophilic substitution reaction, an electrophilic addition reaction, an esterification reaction, or an amidation reaction is separately performed on a C-19 exocyclic double bond or a C-28 carboxyl group to prepare a derivative, and the derivative is used as an active ingredient to prepare a drug having anti-inflammatory and immunomodulatory effects. In the present invention, it is disclosed for the first time that the B4 derivative has the effect of treating inflammatory diseases such as inflammatory bowel disease, atopic dermatitis, eczema, and psoriasis, and the effect is superior to that of B4 or a clinically common drug. In addition, it is also disclosed for the first time that most B4 derivatives do not show obvious cytotoxicity to macrophages, and can reduce the level of P-IκBa protein in LPS and ATP-induced macrophage NF-κB signaling pathways, inhibit the activation of NLRP3 signaling pathways and obviously reduce the level of Pro-IL-1β (p<0.05), i.e., inhibiting the activation of an inflammasome pathway, and the effect is superior to that of B4. These results show that the B4 derivative of the present invention has better anti-inflammatory activity.

The present invention provides a method for constructing a characteristic spectrum of Pulsatilla chinensis medicinal materials, standard decoctions, or traditional Chinese medicine granules, comprising the following steps: preparing a test solution from the Pulsatilla chinensis medicinal materials, standard decoctions, or traditional Chinese medicine granules; and subjecting the test solution to ultra-high performance liquid chromatography analysis. The ultra-high performance liquid chromatography analysis conditions include: mobile phase A being a mixture of acetonitrile and methanol, mobile phase B being an aqueous phosphoric acid solution, and employing gradient elution. The characteristic spectrum constructed by the method described herein is rich in information, highly characteristic, and has good reproducibility.

The invention belongs to the technical field of veterinary drugs, and discloses a Chinese pulsatilla root alcohol extract and gentamicin composition and application thereof in inhibition of drug-resistant salmonella typhimurium. The pulsatilla alcohol extract composition provided by the invention comprises pulsatilla alcohol extract and gentamicin, and for salmonella typhimurium, the optimal ratio concentration of the pulsatilla alcohol extract to the gentamicin is 31.25 mg / mL: 2 [mu] g / ml; the Chinese pulsatilla root alcohol extract and the gentamicin have the synergistic bacteriostatic effect on salmonella typhimurium, the composition reduces the concentration of single bacteriostasis of the Chinese pulsatilla root alcohol extract and the gentamicin, the cost is saved, and the composition has wide application prospects and development value in the fields of food processing, medicine and breeding industry.

The invention relates to the field of traditional Chinese medicines and discloses a traditional Chinese medicine composition for treating damp-heat downward flow type balanitis and urinary tract infection as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 8 to 12 parts of rhizoma anemarrhenae, 8 to 12 parts of cortex phellodendri, 8 to 12 parts of honeysuckle flower, 13 to 17 parts of radix scrophulariae, 8 to 12 parts of rhizoma paridis, 4 to 6 parts of radix gentianae, 4 to 6 parts of radix angelicae, 5 to 7 parts of cicada slough, 25 to 35 parts of semen coicis, 5 to 7 parts of wintersweet flower, 8 to 12 parts of cortex moutan, 13 to 17 parts of radix paeoniae rubra, 8 to 12 parts of cyrtomium rhizome, 13 to 17 parts of herba patriniae, 8 to 12 parts of radix pulsatillae and 8 to 12 parts of liquorice root. The traditional Chinese medicine composition can effectively improve symptoms such as red and swollen glans penis, astringent and painful urination and scorching urethra, remarkably improve the treatment effective rate and effectively control infection, has good safety and low cost, and is suitable for long-term or repeated attack patients to use.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine electuary for intestinal injury caused by radiotherapy and chemotherapy and a preparation method of the traditional Chinese medicine electuary. According to the composition principle of monarch, minister, assistant and guide of a traditional Chinese medicine prescription, the traditional Chinese medicine electuary is prepared by taking radix astragali preparata as a monarch drug, taking radix codonopsis and rhizoma polygonati as minister drugs, and taking poria cocos, bran-fried rhizoma atractylodis macrocephalae, hairyvein agrimony, Chinese pulsatilla root, rhizoma bletillae and pinellia ternate as assistant drugs, and taking pericarpium citri reticulatae, vinegar-processed curcuma zedoary, pseudo-ginseng and musk as assistant drugs through scientific compatibility and a preparation process. The intestinal function can be adjusted, and the intestinal injury symptom caused by radiotherapy and chemotherapy is improved.

The invention provides a traditional Chinese medicine composition for treating avian colibacillosis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations, and the preparation method comprises the following steps: mixing 12-15 parts by weight of scutellaria baicalensis, 9-11 parts by weight of coptis chinensis, 3.5-4.5 parts by weight of honeysuckle, 5-7 parts by weight of andrographis paniculata and 5-7 parts by weight of dandelion, crushing, adding an acidic ethanol solution of which the mass is 8-10 times of that of the mixture, extracting, filtering, and concentrating to obtain a filtrate; first compound liquid is obtained; mixing 11-13 parts by weight of kudzuvine root, 10-12 parts by weight of Chinese pulsatilla root, 4-5 parts by weight of Chinese arborvitae twig and leaf, 6-7 parts by weight of Chinese gall and 7-9 parts by weight of echinacea purpurea, crushing, adding an ethanol aqueous solution of which the mass is 8-10 times that of the mixture, extracting, and filtering to obtain a compound liquid II; and respectively carrying out vacuum rotary evaporation on the composite liquid I and the composite liquid II until the relative density is 1.09-1.13, mixing, and carrying out spray drying to obtain the product. The traditional Chinese medicine composition prepared by the invention is high in effect taking speed and remarkable in curative effect when being used for treating chicken and poultry colibacillosis.

The invention relates to the technical field of medicines, and provides application of pulsatilla saponin B4 in preparation of a medicine for treating osteoarthritis. The pulsatilla saponin B4 can obviously promote the expression of SOX9, which is an important cartilage specific transcription factor, and inhibit the expression of matrix metalloproteinase 13 and disintegrin and metalloproteinase 5 with platelet reaction protein motifs at the same time, thereby finally relieving the degradation of extracellular cartilage matrixes such as II-type collagen and aggregation proteoglycan. Therefore, the pulsatilla saponin B4 can inhibit cartilage degeneration and improve cartilage extracellular matrix metabolic disorder. After the pulsatilla saponin B4 is injected into the abdominal cavity of a mouse with osteoarthritis, the progress of the osteoarthritis is effectively relieved, the side effect is relatively small, and no obvious injury is caused to main organs. The pulsatilla saponin B4 is applied to treatment of osteoarthritis, new application of the pulsatilla saponin B4 is developed, and a new choice is provided for preparation of the medicine for treating osteoarthritis.

The invention relates to the technical field of traditional Chinese medicine composition preparation and application, and discloses a traditional Chinese medicine composition for improving urinary tract infection and a preparation method and application thereof.The traditional Chinese medicine composition is prepared from radix bupleuri, radix scutellariae, raw radix astragali, radix salviae miltiorrhizae, the root of Chinese pulsatilla, herba patriniae, polygonum aviculare, chingma abutilon seeds, the root of three-nerved spicebush, fennel, cinnamon and juncus roemerianus. The traditional Chinese medicine composition comprises the following components in parts by mass: 14-16g of radix bupleuri; 9 to 11 g of radix scutellariae; 19 to 21 g of raw radix astragali; 29 to 31 g of red sage root; 13-17g of Chinese pulsatilla root; 28-32 g of herba patriniae; 14-16 g of polygonum aviculare; 13 to 16 g of chingma abutilon seeds; 7-9g of combined spicebush root; 7-9g of fennel; 5-7g of cinnamon; according to the traditional Chinese medicine composition for strengthening the body resistance, clearing heat and promoting diuresis, the clearing curative effect on RUTI drug-resistant bacteria is better than that of antibiotics, the symptoms of a patient are obviously improved, the recurrence rate is lower than that of an antibiotic group, and the treatment principle and purpose of traditional Chinese medicine are completely different from those of antibiotic treatment; the traditional Chinese medicine composition disclosed by the invention considers recovery and improvement of the overall functional state of a patient instead of purely removing pathogenic bacteria.

The invention relates to application of pulsatilla saponin D in preparation of a medicine for preventing or treating human prostatic hyperplasia. The invention shows that the pulsatilla saponin D has an inhibition effect on human prostate cell WPMY-1, HPRF and BPH-1 cell strains, and can cause cell apoptosis to a certain extent, so that the pulsatilla saponin D shows the effect of preventing or treating prostatic hyperplasia.

The invention belongs to the technical field of traditional Chinese medicines, and provides a traditional Chinese medicine compound composition for treating ulcerative colitis and a preparation method thereof. Chinese pulsatilla root and coptis chinensis are used for clearing away heat and toxic materials, cooling blood and stopping dysentery; bletilla striata has the effects of astringing to stop bleeding, diminishing swelling and promoting granulation, fried sanguisorba officinalis has the effects of cooling blood, stopping bleeding, detoxifying and astringing sores, golden cypress has the effects of clearing heat and drying dampness, and ash bark has the effects of inducing astringency and stopping dysentery, all serve as ministerial drugs, assist the monarch drugs to play roles, enhance the curative effects of the monarch drugs and assist the monarch drugs in purging fire and detoxifying. The fried radix paeoniae alba is used for regulating menstruation and relieving pain, the root of Chinese toona astringents and stopping bleeding, and the radix scutellariae is used for clearing away heat and toxic materials, which are all adjuvant drugs. The liquorice serves as a conductant drug and can relieve spasm and pain and resolve the drugs. All the traditional Chinese medicine components are in synergistic interaction, so that the traditional Chinese medicine compound composition has the effects of clearing away heat and toxic materials, astringing intestines and stopping bleeding, and astringing sores and promoting tissue regeneration, and can be used for treating ulcerative colitis.

The invention relates to a fermented traditional Chinese medicine composition for preventing and treating piglet viral diarrhea as well as a preparation method and application thereof, and relates to the technical field of livestock breeding. The feed is prepared by taking traditional Chinese medicine components as basic raw materials and adding probiotics for fermentation, and the traditional Chinese medicine components comprise the following raw materials in parts by weight: 0.5-1.5 parts of cardamine violifolia dry powder, 5-15 parts of Chinese pulsatilla root, 10-20 parts of coptis chinensis, 10-20 parts of golden cypress, 10-20 parts of ash bark, 4-8 parts of turmeric, 6-10 parts of pumpkin seeds and 4-8 parts of a ginkgo leaf extract feed additive. Through cooperation of multiple components, the immunity and antioxidant functions of the body are enhanced, the structural integrity of the intestinal tract is maintained, piglet diarrhea caused by PEDV infection is effectively relieved, and growth and development of the piglet are promoted; local specialty crops are selected as medicines, and theoretical basis and practical reference are provided for deep processing of local genuine medicinal materials and PED prevention and control.

The present invention discloses a compound pharmaceutical composition, a preparation method thereof, and an application thereof in the preparation of a drug for reducing the infection rate of Dongfeng snail egg cysts or improving the hatching rate. According to weight parts, the compound pharmaceutical composition includes: 10 to 15 parts of a Chinese medicine compound composition and 5 to 10 parts of cecropin; according to weight parts, the Chinese medicine compound composition is made of the following raw materials, the raw materials including: 10 to 30 parts of Pulsatilla striata, 20 to 30 parts of Macleaya cordata, 10 to 20 parts of Osmanthus fragrans, 10 to 20 parts of Rhizoma Dioscoreae, 10 to 30 parts of Dictamni, 20 to 30 parts of Rhizoma Dioscoreae, 5 to 10 parts of Trichosanthes kirilowii, and 5 to 15 parts of Erigeron breviscapus. The compound pharmaceutical composition can significantly inhibit the red, white, and yellow infection of Dongfeng snail egg cysts, effectively prevent large-scale cross-infection of egg cysts, reduce the infection rate of egg cysts, and improve the hatching rate of Dongfeng snail egg cysts, thereby improving economic benefits.

The invention relates to the technical field of preparation of feed additives, in particular to a traditional Chinese medicine compound feed additive for preventing stress syndromes of early weaned piglets and a preparation method of the traditional Chinese medicine compound feed additive. The medicine specifically comprises the following components: rhizoma osmundae, astragalus membranaceus, mangnolia officinalis, hawthorn, medicated leaven, pericarpium citri reticulatae, polygala tenuifolia, Chinese pulsatilla root, cortex fraxini, poria cocos, liquorice and spina date seed. The traditional Chinese medicine compound feed additive provided by the invention has a remarkable technical effect in the aspect of preventing the early-stage weaned piglet stress syndrome. Through scientific compatibility of various traditional Chinese medicine components, the additive can effectively improve the digestive function of the weaned piglets, enhance the immunity, relieve the stress response and remarkably reduce the diarrhea rate and the death rate. Experimental results show that piglets using the additive are superior to a blank control group and other substitution groups in the aspects of weight gain, oxidation resistance, serum hormone level, immune globulin indexes and the like, and the additive is proved to have a comprehensive physiological regulation function.

The application discloses a medicine composition for treating infant night cough, which is composed of Radix Bupleuri, Radix Paeoniae Alba, Fructus Aurantii Immaturus, roasted Germinated Millet, roasted Millet, Pericarpium Citri Reticulatae, Folium Pyrrosiae, Radix Trichosanthis, Radix Pulsatillae, Radix Anemarrhenae, Radix Peucedani, Radix Trichosanthis, Radix Ophiopogonis, Radix Sophorae Immaturus, and Radix Glycyrrhizae. The medicine composition can be added with pharmaceutically acceptable adjuvants to form preparations. The medicine composition and the preparations can be used for preparing medicines for treating infant night cough.

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for treating radiation proctitis and a preparation method thereof.The traditional Chinese medicine composition is prepared from rhizoma coptidis processed with wine, radix scutellariae slices, radix rehmanniae recen, baked ginger, rhizoma atractylodis macrocephalae fried with bran, carbonized sanguisorba root, the root of Chinese pulsatilla, rhizoma bletillae, the root of kudzu vine, astragalus membranaceus, carbonized hair, honey-fried licorice root, radix paeoniae alba and rhizoma nelumbinis charcoal. According to the principle of clearing heat, promoting diuresis, detoxifying, cooling blood, dispersing blood stasis and stopping bleeding in traditional Chinese medicine, the pathological states of internal exuberance of fire-heat toxins and burns intestinal collaterals are comprehensively conditioned. Clinical research proves that the medicine can improve the traditional Chinese medicine syndrome score of patients suffering from stomachache, diarrhea, mucous bloody stool type rectum mucosa edema, hyperemia, bleeding and even ulceration and ulceration caused by radiotherapy, has practical popularization and application value, and is worthy of popularization and application. Therefore, the problems that the existing western medicine for treating radiation proctitis can only temporarily relieve symptoms, cannot repair intestinal mucosa injury and is easy to cause drug resistance and intestinal flora imbalance are solved.

The application relates to the technical field of traditional Chinese medicine preparation, and particularly discloses a compound traditional Chinese medicine preparation for targeting egg duck and goose egg cytoplasmic disease, which comprises the following components: white head, Coptis chinensis, Huangqi, Phellodendron amurense, wine Danpi, bran-fried peach kernel, Chinese perilla, pomegranate peel, liquorice, honeysuckle, Andrographis, figwort, emblic, ginger, fried atractylodes, fried safflower, Xianhecao, spiny amaranth, and Poria cocos. Through the combination of monarch, minister, assistant and messenger, the core effects of heat-clearing and detoxifying, blood-cooling and dysentery-stopping, and dampness-drying and insect-killing of the medicines are synergistically exerted, the pathogenesis characteristics of the egg cytoplasmic disease are accurately targeted, blood stasis and blood circulation are promoted to eliminate the swelling of the lesion, and the intestinal mucosa damage is repaired to stop bleeding, the symptoms of hematochezia, diarrhea and oviduct inflammation of the egg duck and goose caused by the egg cytoplasmic disease are effectively relieved, the problems of the decreased antibiotic effect, drug residue, bacterial flora disorder and food safety risk are effectively solved, and the compound traditional Chinese medicine preparation has the remarkable progress of drug resistance, safety, no residue, intestinal protection and green environmental protection.

The invention discloses an anti-tumor traditional Chinese medicine compound capable of removing three flavors. The anti-tumor traditional Chinese medicine compound is prepared from radix puerariae, radix scutellariae, rhizoma coptidis, cortex phellodendri, rhizoma atractylodis, radix angelicae sinensis, radix aucklandiae, radix pulsatillae, herba hedyotidis diffusae, rhizoma atractylodis macrocephalae and raw liquorice Compared with the original formula of compound radix puerariae decoction, the three-component-removing anti-tumor traditional Chinese medicine compound has the advantages that the three traditional Chinese medicines of gentiana macrophylla, rhizoma alismatis and betel nut which do not have the effects of clearing away heat and toxic materials and tonifying spleen and do not enter lung meridians are removed, the three-component-removing anti-tumor traditional Chinese medicine compound is obtained, and research finds that the formula still has the anti-colorectal cancer efficacy, the anti-breast cancer efficacy is increased, and the anti-tumor effect is good. Compared with the original formula of the compound radix puerariae decoction, the compound radix puerariae decoction has higher safety.

The invention discloses a traditional Chinese medicine composition for treating inflammatory bowel disease as well as a preparation method and application thereof, and belongs to the technical field of medicines. The traditional Chinese medicine composition is prepared from the following components in parts by mass: 15-17 parts of radix pulsatillae, 10-12 parts of cortex fraxini, 10-12 parts of wine-processed rhizoma coptidis, 30-32 parts of cortex phellodendri, 15-17 parts of radix paeoniae alba, 10-12 parts of radix angelicae sinensis, 10-12 parts of radix aucklandiae and 10-12 parts of liquorice root. The traditional Chinese medicine composition provided by the invention is prepared from natural medicines, has low side effects, and provides a new choice for rehabilitation treatment and prevention of patients with inflammatory bowel diseases.

The invention discloses a traditional Chinese medicine composition for treating constipation as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The composition comprises the following components in parts by weight: 45 to 85 parts of Chinese pulsatilla root, 45 to 85 parts of oldenlandia diffusa, 45 to 85 parts of sculellaria barbata, 80 to 140 parts of Chinese actinidia root, 45 to 85 parts of trogopterus dung, 80 to 140 parts of coix seed, 80 to 140 parts of poria cocos, 80 to 140 parts of rhizoma pinellinae praeparata, 45 to 85 parts of pericarpium citri reticulatae, 60 to 100 parts of bighead atractylodes rhizome, 45 to 85 parts of endothelium corneum gigeriae galli, 60 to 100 parts of white hyacinth bean, 60 to 100 parts of dried ginger and 45 to 85 parts of liquorice. The traditional Chinese medicine composition has a good treatment effect on constipation caused by gastrointestinal tumors, is mild in medicine property, and is specially developed for patients with constipation caused by tumors.

The invention belongs to the technical field of traditional Chinese medicines, and discloses application of pulsatilla saponin B4 in preparation of a medicine for treating intervertebral disc degeneration and discogenic pain. The pulsatilla saponin B4 is used for preparing the medicine for treating the intervertebral disc degeneration and the discogenic pain, the intervertebral disc degeneration can be treated, the discogenic pain can be relieved, and the effect is good. The pulsatilla saponin B4 disclosed by the invention is used for preparing the medicine for treating intervertebral disc degeneration and intervertebral disc-derived pain, and the pulsatilla saponin B4 has small influence on cell activity, has no cytotoxicity and is good in medicine safety.

The invention discloses a traditional Chinese medicine composition for efficiently preventing and treating coccidiosis of livestock as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese veterinary medicines. The traditional Chinese medicine composition is prepared from the following raw material components in parts by weight: 60 to 80 parts of sweet wormwood herb, 30 to 40 parts of radix sophorae flavescentis, 20 to 25 parts of rhizoma coptidis, 25 to 30 parts of radix pulsatillae, 30 to 35 parts of fried flos sophorae, 35 to 40 parts of radix sanguisorbae, 50 to 60 parts of cacumen biotae and 25 to 35 parts of fructus tsaoko. The traditional Chinese medicine composition provided by the invention has a good prevention effect on sub-clinical and clinical large intestine damp-heat diarrhea caused by coccidiosis and spleen-deficiency damp-heat diarrhea caused by repeated attack of coccidiosis and long-term accumulation of coccidiosis. The traditional Chinese medicine composition can treat symptoms and causes, improve the self-healing function of livestock and promote natural immunity of cattle and sheep, is small in toxic and side effects, high in safety, capable of being evenly mixed with daily ration to be orally taken in a group, convenient to use and easy for group prevention and group control of coccidiosis, and can also solve the problems of drug resistance and residues in traditional drug treatment.

The invention discloses application of a triterpenoid saponin extract in preparation of a medicine for enhancing the effect of radiotherapy, and belongs to the technical field of biomedicine.The Chinese pulsatilla saponin D (SB365) in the formula (I) is a novel triterpenoid saponin extract extracted and identified from Chinese pulsatilla roots, is small in toxic and side effect, can induce apoptosis of liver cancer cells, and can be used for preparing a medicine for enhancing the effect of radiotherapy. A good proliferation inhibition effect is achieved on liver cancer cells; on the other hand, the SB365 can inhibit an HR pathway by inhibiting an AKT / ATM / DDR signal transduction axis and performing targeted combination with RAD51 protein, so that the sensitivity of ionizing radiation to hepatocellular carcinoma is improved.

The invention discloses a pharmaceutical composition for treating diarrhea of yak calves, and relates to the technical field of biological pharmacy, the pharmaceutical composition is prepared from the following raw materials: coptis chinensis, cortex phellodendri, cortex fraxini, Chinese pulsatilla root, purslane, Chinese yam, poria cocos, bighead atractylodes rhizome and liquorice, various natural traditional Chinese medicinal materials are adopted as the raw materials, and the pharmaceutical composition is safe and free of side effects; and adverse effects on the meat quality of the yak calves and the quality of dairy products are avoided. The invention also discloses a preparation method of the pharmaceutical composition for treating diarrhea of yak calves. The preparation method comprises the following steps: step 1, weighing raw materials; 2, pretreating the raw materials; step 3, adding water for soaking; step 4, first heating reflux extraction; step 5, filtering for the first time; step 6, adding water for the second time and extracting; step 7, filtering for the second time; step 8, concentration treatment; the preparation method is easy to operate, effective components in the raw materials can be extracted to the maximum extent through reasonable extraction and concentration processes, and the curative effect of the pharmaceutical composition is improved.

The invention belongs to the technical field of medicines, discloses a synergistic pharmaceutical composition based on pulsatilla saponin and bifendate and application of the synergistic pharmaceutical composition in treatment of acute liver injury, and particularly discloses a pharmaceutical composition which comprises pulsatilla saponin B4 and bifendate or dexamethasone. The invention provides a pharmaceutical composition containing pulsatilla saponin B4 (BS-B4) and a drug bifendate or dexamethasone combined with the pulsatilla saponin B4, and experiments prove that the pharmaceutical composition has good effects of preventing and treating acute liver injury and can effectively reduce spleen and liver indexes and expression of serum liver injury markers (ALT, AST and T-Bil-V). The pharmaceutical composition can be used for effectively relieving acute liver injury induced by carbon tetrachloride by regulating inflammatory factors (IL-1beta, TNF-alpha and IL-10) and oxidative stress pathways (MDA and GSH-Px).

The invention discloses application of a triterpenoid saponin extract in preparation of a medicine for treating liver cancer, and belongs to the technical field of biomedicine.The Chinese pulsatilla saponin D (SB365) shown in the formula (I) is a novel triterpenoid saponin extract extracted and identified from Chinese pulsatilla roots, is small in toxic and side effect, can induce liver cancer cell apoptosis and has a good proliferation inhibition effect on liver cancer cells; on the other hand, the SB365 can inhibit an HR pathway by inhibiting an AKT / ATM / DDR signal transduction axis and combining RAD51 protein in a targeted manner, so that the sensitivity of oxaliplatin to hepatocellular carcinoma is improved.

The invention provides a Chinese pulsatilla root oral liquid preparation and a preparation method thereof.The Chinese pulsatilla root oral liquid preparation per liter comprises 300 g of Chinese pulsatilla roots, 150 g of rhizoma coptidis, 300 g of cortex fraxini, 225 g of cortex phellodendri, 50 ml of PEG-400, 20 g of xanthan gum, 20 g of hydrophilic colloidal silicon dioxide, 5 g of neotame, 1.5 g of ethylparaben and the balance auxiliary materials. The palatability is better, and the clinical effect is obviously improved compared with that of other similar products.

The invention discloses an anemoside B4 derivative as well as a preparation method and application thereof, a compound anemoside B4 (abbreviated as B4 or AB4) is taken as a raw material, and the derivative is prepared by respectively carrying out nucleophilic substitution, electrophilic addition, esterification reaction or amidation and other reactions on C-19 exocyclic double bonds or C-28 carboxyl. The compound is used as an active component to prepare medicines with anti-inflammatory and immunoregulation effects. The invention discloses the effect of the B4 derivative on treating inflammatory diseases such as inflammatory bowel disease, atopic dermatitis, eczema and psoriasis for the first time, and the drug effect of the B4 derivative is superior to that of B4 or clinically common drugs. In addition, the invention also discloses that most B4 derivatives do not show obvious cytotoxicity to macrophages for the first time, can reduce the P-I kappa Ba protein level in LPS and ATP induced macrophage NF-kappa B signaling pathways, inhibit the activation of NLRP3 signaling pathways and obviously reduce the Pro-IL-1beta level (p is less than 0.05), namely inhibit the activation of inflammasome pathways, and the effect is superior to that of B4; the results show that the B4 derivative disclosed by the invention has better anti-inflammatory activity.

The invention relates to the technical field of veterinary drugs, and discloses a traditional Chinese veterinary drug for treating diarrhea of newborn livestock and a preparation method and application of the traditional Chinese veterinary drug. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-30 parts of poria cocos peel, 10-30 parts of scutellaria baicalensis, 20-80 parts of poplar flower, 10-60 parts of schisandra chinensis, 5-20 parts of Chinese pulsatilla root, 5-20 parts of white paeony root and 10-30 parts of purslane. According to the traditional Chinese veterinary medicine for treating diarrhea of the newborn livestock and the preparation process and application thereof, the veterinary medicine obtained through the steps is prepared from Chinese herbal medicines, so that stimulation and harm of the medicine to the newborn livestock can be reduced, meanwhile, the aim of preventing diarrhea is achieved according to the characteristics of the Chinese herbal medicines, and therefore the higher survival rate of the newborn livestock is ensured; meanwhile, normal lactation of the newborn livestock is not affected, so that the immunity of the newborn livestock is improved, and the preparation method is simple in steps and easy to operate in the preparation process of the traditional Chinese veterinary medicine, so that the production cost is reduced.