11 results about "Antibiotic effect" patented technology

The invention provides a multi-mode Raman spectrum data deep fusion-based bacterial drug resistance prediction method and system. The method comprises the following steps: acquiring static Raman spectrum data of target bacteria and target antibiotics and dynamic Raman spectrum data of the target bacteria under the action of the target antibiotics; respectively carrying out embedded coding and feature extraction on the static Raman spectrum data and the dynamic Raman spectrum data to obtain static spectrum features and dynamic spectrum features; and performing deep cross fusion on the static spectral features and the dynamic spectral features, and obtaining a drug resistance prediction result of the target bacteria in combination with a multi-layer perceptron. According to the technical scheme, the limitation that traditional drug sensitivity detection is long in time consumption and single in information is broken through, and a novel efficient, accurate and explainable drug resistance monitoring scheme is provided for clinic.

The invention discloses a high-throughput bacterial drug sensitivity test platform based on computer vision. The platform integrates the functions of automatic separation and identification of bacteria, drug sensitivity test and result analysis. Separation and identification of bacteria are realized through a microfluidic technology, and growth conditions of the bacteria under the action of different antibiotics are monitored in real time in combination with a computer vision technology, so that the antibiotic sensitivity of the bacteria is rapidly judged. The platform has the characteristics of high throughput, automation and intellectualization, can complete detection of a large number of samples in a short time, automatically generates a drug sensitivity test report, and provides powerful support for clinical diagnosis and treatment. By implementing the method, the speed and the accuracy of the bacterial drug sensitivity test are remarkably improved, and the method has a wide application prospect.

The present invention belongs to the technical field of biomedicine, and relates to use of linoleic acid in the preparation of a drug for improving the sensitivity of Gram-positive bacteria to an antibiotic. It has been discovered for the first time that the combined use of linoleic acid and an antibiotic can significantly improve the sensitivity of Gram-positive bacteria to the antibiotic. In vivo study data have shown that the combination of linoleic acid and the antibiotic can not only improve the survival rate of mice infected with Gram-positive bacteria, but also enhance the ability of the mice to clear Gram-positive bacteria. Furthermore, formulating linoleic acid and the antibiotic into an anti-infection composition can, in one aspect, achieve a significant clinical anti-infection effect, and in another aspect, prolong the post-antibiotic effect. Moreover, linoleic acid is an essential fatty acid in human and animal nutrition, with high nutritional value, and it has been widely used in food and pharmaceutical products, demonstrating high safety.

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

The application relates to the technical field of traditional Chinese medicine preparation, and particularly discloses a compound traditional Chinese medicine preparation for targeting egg duck and goose egg cytoplasmic disease, which comprises the following components: white head, Coptis chinensis, Huangqi, Phellodendron amurense, wine Danpi, bran-fried peach kernel, Chinese perilla, pomegranate peel, liquorice, honeysuckle, Andrographis, figwort, emblic, ginger, fried atractylodes, fried safflower, Xianhecao, spiny amaranth, and Poria cocos. Through the combination of monarch, minister, assistant and messenger, the core effects of heat-clearing and detoxifying, blood-cooling and dysentery-stopping, and dampness-drying and insect-killing of the medicines are synergistically exerted, the pathogenesis characteristics of the egg cytoplasmic disease are accurately targeted, blood stasis and blood circulation are promoted to eliminate the swelling of the lesion, and the intestinal mucosa damage is repaired to stop bleeding, the symptoms of hematochezia, diarrhea and oviduct inflammation of the egg duck and goose caused by the egg cytoplasmic disease are effectively relieved, the problems of the decreased antibiotic effect, drug residue, bacterial flora disorder and food safety risk are effectively solved, and the compound traditional Chinese medicine preparation has the remarkable progress of drug resistance, safety, no residue, intestinal protection and green environmental protection.

The invention relates to the technical field of chemical application, in particular to application of an SCQDs fluorescent probe in the field of antibiotic detection. When the SCQDs fluorescent probe disclosed by the invention acts with antibiotics, the fluorescence intensity is obviously reduced, the addition of coexisting metal ions does not cause obvious change, F / F0 is still maintained at a relatively low level, and the SCQDs fluorescent probe disclosed by the invention shows excellent anti-interference capability. According to the invention, the SCQDs-TC fluorescent probe is constructed, and the high-selectivity recognition of TC is realized by utilizing the fluorescence quenching effect of TC on SCQDs. According to the invention, the SCQDs-Fe < 3 + >-LEV fluorescent switch probe is constructed, and the specific detection of LEV is realized by using the recovery effect of LEV on Fe < 3 + >-induced fluorescence quenching.

This application proposes a method and related apparatus for detecting antimicrobial susceptibility based on the physical morphological characteristics of single bacteria. The method includes: S1, establishing an independent detection unit; S2, drug processing; S3, single-bacterial imaging; S4, single-bacterial segmentation; S5, multidimensional feature extraction; S6, single-index performance evaluation; S7, joint modeling and performance evaluation; and S8, result output. This application can establish a rapid drug susceptibility discrimination process for common Gram-negative bacteria in the early single-bacterial morphological changes after antibiotic treatment, thereby improving the interpretability, standardization potential, and clinical translational applicability of the method while shortening the detection time.

The present invention belongs to the technical field of biomedicine, and particularly relates to the application of linoleic acid in the preparation of a medicament for enhancing the antibiotic sensitivity of Gram-positive bacteria. The present invention discovers for the first time that the combination of linoleic acid and an antibiotic can significantly enhance the antibiotic sensitivity of Gram-positive bacteria; in vivo research data shows that the combination of linoleic acid and an antibiotic can not only improve the survival rate of mice infected with Gram-positive bacteria, but also enhance the ability of mice to eliminate Gram-positive bacteria; further, an anti-infective composition prepared from linoleic acid and an antibiotic can, on the one hand, achieve a significant clinical anti-infective effect, and on the other hand, prolong the post-antibiotic effect; moreover, linoleic acid is an essential fatty acid in human and animal nutrition, has high nutritional value, and has been widely used in foods and drugs, with high safety.

The application provides a bacterial drug resistance prediction method and system based on multimodal Raman spectrum data deep fusion, comprising: obtaining static Raman spectrum data of target bacteria and target antibiotics and dynamic Raman spectrum data of target bacteria under the action of target antibiotics; embedding coding and feature extraction are respectively performed on the static Raman spectrum data and the dynamic Raman spectrum data to obtain static spectrum features and dynamic spectrum features; the static spectrum features and the dynamic spectrum features are deeply cross-fused to obtain a drug resistance prediction result of the target bacteria by combining a multilayer perception machine. The technical scheme breaks the limitation of long time consumption and single information of traditional drug sensitivity detection, and provides a new drug resistance monitoring scheme which is efficient, accurate and interpretable for clinic.

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine formula for treating cough caused by mycoplasma pneumoniae infection, which comprises the following raw materials in parts by weight: 10g of ephedra, 10g of semen armeniacae amarae, 10g of platycodon grandiflorum, 10g of liquorice, 10g of fructus perillae, 10g of rhizoma pinellinae praeparata, 10g of mangnolia officinalis, 10g of poria cocos, 10g of scutellaria baicalensis, 10g of lumbricus, 10g of inula flower (decocted in a wrapped manner), 15g of uncaria (decocted later) and 5g of ginger. Based on the theory of traditional Chinese medicine, aiming at the key pathogenesis of wind-heat affecting lung and lung dysfunction (lung qi is mainly not lowered), the traditional Chinese medicine components are matched with one another, so that frequent irritant cough is quickly relieved, the course of cough is shortened, and a patient gets rid of cough trouble as soon as possible. The traditional Chinese medicine prescription does not depend on an antibiotic action mechanism, provides a brand new and effective medication selection for clinical treatment, and fills the blank of a targeted traditional Chinese medicine prescription for treating cough caused by mycoplasma pneumonia infection. And a new hope and a new choice are brought to a patient who is deeply suffered from cough impairment caused by mycoplasma pneumoniae infection.

The present invention relates to compositions, kits and methods using combinations of surfactants or cell wall-hydrolyzing enzymes with antibiotics to potentiate the antibiotic effect against bacterial infections. In some embodiments, the surfactant is a pore-forming biosurfactant such as rhamnolipids and the antibiotic is an aminoglycoside antibiotic such as tobramycin.