14 results about "Multiple drug resistance" patented technology

The utility model discloses a multi-bin paper-based micro-fluidic chip for visual detection of bacterial drug resistance. The multi-bin paper-based micro-fluidic chip comprises a hydrophilic paper base, the detection mechanism is a hydrophilic channel established on the hydrophilic paper base; the detection mechanism comprises a sample adding bin and an anti-overflow bin, and the appearance of the anti-overflow bin is circular; one end of the anti-overflow channel is communicated with the sample adding bin, and the other end of the anti-overflow channel is communicated with the anti-overflow bin; the appearance of each reaction bin is an isosceles trapezoid; the plurality of reaction bins are uniformly distributed in a fan shape by taking the circle center of the sample adding bin as a central original point; one end of each diffusion channel is communicated with the reaction bin, and the other end of each diffusion channel is communicated with the sample adding bin; wherein the diffusion channels and the reaction bins are arranged in a one-to-one correspondence manner; the hydrophobic barrier structure is a hydrophobic area formed on the hydrophilic paper base through wax spraying printing along the outer edge of the detection mechanism. According to the multi-bin paper-based micro-fluidic chip for visual detection of bacterial drug resistance, provided by the utility model, rapid, high-throughput and low-cost detection of multiple bacterial drug resistance can be realized.

The application discloses application of antibacterial peptide BMAP-28 and antibiotics in combined preparation of antibacterial drugs and belongs to the technical field of biological medicine. The antibiotics are selected from one or more of the following: ciprofloxacin, gentamicin and cefixime. The application combines the antibacterial peptide BMAP-28 with antibiotics such as ciprofloxacin and gentamicin, finds that the combination has a significant synergistic antibacterial effect on Enterobacter sakazii, can greatly save the dosage of the drug, reduce the toxic side effect and block the evolution process of drug resistance. Through the destruction of the bacterial cell membrane and the assistance of the antibiotic effect, the combination can effectively overcome the multiple drug resistance, widen the antibacterial spectrum and has a wide clinical application prospect.

The application discloses K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies, hybridoma cell strains thereof and application of the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies, sequence information of the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies is shown as SEQ NO:1-SEQ NO:56, preservation numbers of the hybridoma cell strains are CCTCC:C2023365, CCTCC:C2023366, CCTCC:C2023367, CCTCC:C2024340, CCTCC:C2024341, CCTCC:C2024342 and CCTCC:C2024343 respectively, the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies provided by the application can be specifically combined with capsular polysaccharide of Klebsiella, are favorable for typing of Klebsiella pneumoniae in clinic, and can prove excellent effects of the K64 type Klebsiella pneumoniae capsular polysaccharide monoclonal antibodies in preventing and treating infection of Klebsiella pneumoniae in in-vivo and in-vitro tests, and the application provides a way for solving problems of Klebsiella pneumoniae infection and multiple drug resistance, has remarkable significance for preventing, diagnosing and treating Klebsiella pneumoniae infection, and has important value for developing a new generation of Klebsiella pneumoniae drugs.

This invention belongs to the field of biodetection technology, specifically disclosing a primer-probe composition and kit for detecting drug-resistant gene mutations in Helicobacter pylori. The composition comprises two sets of primers and probes, using the probe melting curve method as its principle. The composition and universal PCR reagents are used to detect multiple drug resistance mutations in the quinolone resistance gene gyrA and the clarithromycin resistance gene 23S rRNA of Helicobacter pylori in biological samples. This overcomes the limitation of existing methods that only detect single mutation sites in a single tube for Helicobacter pylori drug resistance genes, providing a new, rapid, and efficient method for detecting Helicobacter pylori drug resistance genes.

The application provides a multifunctional nano drug delivery system based on iron-doped MOF and a preparation method and application thereof. The system comprises: an Fe / MOF core loaded with a chemotherapeutic drug and an HO-1 inhibitor; a PDA coating layer coated on the surface of the Fe / MOF core for providing near-infrared light heat conversion capability and surface modification interface; and E3 aptamer covalently coupled on the PDA coating layer, constituting a synergistic nano platform with active targeting, pH / NIR dual-responsive drug release, chemical kinetics therapy, ferroptosis sensitization, photothermal therapy and immune regulation functions. The nano system can realize efficient accumulation at the tumor site through active targeting mediated by E3 aptamer, and controllably release drugs under an acidic microenvironment or near-infrared light. A new strategy for multi-mechanism synergistic treatment is provided to overcome the multiple drug resistance and immunosuppression of mCRPC, and has important clinical application prospects.

An antifungal compound / conjugate is derived from quercetin, a naturally occurring flavonoid, through a strategic conjugation approach. The resulting quercetin conjugate exhibits enhanced antifungal activity and improved pharmacological properties compared to its parent molecule. The conjugate demonstrates potent efficacy against a range of drug-resistant fungal pathogens, including Candida auris, a critical threat in clinical settings due to its multidrug resistance. The invention also encompasses methods for synthesizing the conjugate, formulating the conjugate in a pharmaceutical preparation, and methods of use for treatment and prevention of superficial and systemic fungal infections.

The invention relates to the technical field of micro-fluidic electrochemical detection, in particular to a multi-channel micro-fluidic chip for detecting various drug-resistant mycobacterium tuberculosis as well as a use method and application of the multi-channel micro-fluidic chip. The multi-channel micro-fluidic chip comprises a splitting area and a sample mixing area, the splitting area comprises a sample injection hole and a DNA splitting chamber which are communicated in sequence, the sample mixing area comprises a sample mixing chamber, and the DNA splitting chamber and the sample mixing chamber are communicated through a first micro-channel; the electrode array area comprises a plurality of reaction detection chambers and detection electrodes, the detection electrodes are in one-to-one correspondence with the reaction detection chambers and are in contact with the inner spaces of the reaction detection chambers, the electrodes are modified with signal amplification probes, Cas12a-crRNA compounds are preloaded on the surfaces of the electrodes, and the reaction detection chambers are communicated with the sample mixing chamber through a second micro-channel. Multiple channels are designed, synchronous detection of multiple drug-resistant genes is achieved, integration of clinical sample treatment, DNA acquisition, DNA thermal denaturation treatment and DNA detection is achieved, operation is easy and smooth, and clinical diagnosis and medication guidance of tuberculosis drug resistance can be well served.

The invention relates to the technical field of streptococcus suis drug resistance gene detection, and particularly discloses a streptococcus suis drug resistance gene sextuple PCR detection kit and application thereof. The core of the kit is a specific primer combination, the nucleotide sequences of the specific primer combination are as shown in SEQ ID NO: 1-12, and the specific primer combination respectively aims at six key drug-resistant genes of streptococcus suis: ermB, tet (O), patB, aac (6 '')-Ie-ah (2 ')-Ia, optrA and lsa (E). By optimizing the primer concentration and reaction conditions, an efficient and stable sextuple PCR detection system is established, and amplification and detection of the six genes can be synchronously completed in a single reaction. The method has the advantages of high specificity, high sensitivity, good repeatability, simplicity and convenience in operation and the like. A high-throughput and low-cost molecular detection tool is provided for rapid diagnosis, medication guidance and epidemiological monitoring of streptococcus suis clinical multiple drug resistance, and the application prospect is wide.

The invention provides a method for simultaneously and rapidly detecting vibrio parahaemolyticus and multiple drug-resistant genes thereof, which comprises the following steps: preparing vibrio parahaemolyticus immunomagnetic beads by coupling biotinylated vibrio parahaemolyticus polyclonal antibodies with streptavidin nano magnetic beads, and carrying out immunocapture on the vibrio parahaemolyticus. According to the present invention, the RPA-coupled micro-fluidic detection system is designed, the capture condition and the nucleic acid extraction process are optimized, the reasonable RPA-coupled micro-fluidic detection system is designed, the toxR gene of the vibrio parahaemolyticus and the three most common drug-resistant genes floR, sul1 and qnrA are synchronously detected, and the important significance is provided for solving the drug resistance problem of marine product pathogenic microorganisms and ensuring the human health.

This invention belongs to the field of hydrogel dressing technology, specifically disclosing a hydrogel dressing loaded with fresh tea leaf nanoparticles, its preparation method, and its application. The preparation method includes the following steps: preparing fresh tea leaf nanoparticles; preparing a carboxymethyl chitosan solution; preparing a carboxymethyl chitosan solution containing fresh tea leaf nanoparticles; preparing an oxidized hyaluronic acid solution; and mixing the obtained carboxymethyl chitosan solution containing fresh tea leaf nanoparticles with the oxidized hyaluronic acid solution to obtain the hydrogel dressing loaded with fresh tea leaf nanoparticles. This invention utilizes the above-mentioned hydrogel dressing loaded with fresh tea leaf nanoparticles, its preparation method, and its application. The preparation process is simple and easy to control, and is not limited by problems such as increased antibiotic resistance and frequent dressing changes. It promotes wound healing, eliminates harmful bacteria, effectively resists multidrug-resistant bacterial infections, and simultaneously promotes wound healing and skin regeneration.

The present application relates to the field of biotechnology, in particular to a fusion plasmid and its application in gene transfer and protein expression. The present application provides a novel IncI1 and IncHI2 / IncHI2A type self-transmissible fusion plasmid containing NDM-13 and OXA-10 from clinical Salmonella, which also carries other multiple drug resistance genes and shows resistance to multiple antibiotics. Salmonella is a pathogenic bacterium of zoonosis and has the ability to infect humans and livestock. Salmonella carrying the plasmid has the potential to cause outbreaks, and active research is needed to prevent the occurrence of outbreaks.

The present application relates to the technical field of biological medicine, in particular to an antifungal drug composition based on (1,3)-beta-D-glucan synthase inhibitor and application thereof. In vivo and in vitro experiments prove that the combination of endogenous antibacterial peptide LL-37 and (1,3)-beta-D-glucan synthase inhibitor (such as caspofungin, micafungin, anidulafungin, rezafungin and albaconazole) shows significant synergistic inhibitory effect on multiple drug-resistant pathogenic fungi such as Candida albicans and Candida auris. Since free antibacterial peptides are easily degraded by proteases, the present application is based on the synergistic killing mechanism of breaking the network barrier of fungal cell wall and assisting endogenous defense peptide to target cell membrane, and ingeniously uses the "antibacterial peptide LL-37 expression promoter" to actively up-regulate the synthesis of host natural peptides, which provides a new drug regimen for clinical treatment of deep fungal infection.

The application discloses a gene and drug resistance mutation detection method based on a double-site nucleic acid circuit probe and belongs to the technical field of molecular biology. In order to solve the technical problems of false positive detection results, complex operation, high requirements for detection personnel and high cost in the prior art gene and drug resistance mutation detection method, the application provides a gene and drug resistance mutation detection method based on a double-site nucleic acid circuit probe. The method is based on isothermal amplification technology combined with one or more nucleic acid circuits. The isothermal amplification technology includes but is not limited to NASBA, SDA, LAMP, HDA and RPA. The nucleic acid circuit includes but is not limited to a cascade strand displacement nucleic acid circuit and a one-step strand displacement nucleic acid circuit. The detection method provided by the application can simultaneously detect multiple drug resistance mutation sites through a single tube and a single reaction, realizes pathogen detection and pathogen drug resistance mutation detection on unknown samples, has high detection accuracy, is simple in steps and low in cost.

This invention relates to a composition and method for the detection of Tuberculosis drug resistance by multiplex small amplicon based, targeted Next-Gen sequencing (tNGS) using ribonucleotide bases in the primers. It also provides a bioinformatic data analysis tool, for the prediction of drug resistance / susceptibility towards anti- tuberculosis therapy drugs. The invention provides an efficient, cost-effective solution for identifying drug resistance / susceptibility right after tuberculosis diagnosis. In addition, a short read NGS platform with massive parallel sequencing, along with data analysis of this invention, offers high sensitivity and specificity for detection of drug resistance.