21 results about "Membrane transport" patented technology

The application discloses a single-particle layer metal organic framework mixed matrix nanometer porous membrane and a preparation method thereof, and belongs to the field of functional materials and membrane separation technology. The preparation method comprises the following steps: firstly, synthesizing MOF nanoparticles; then, self-assembling the MOF particles and polystyrene balls on the water surface; after treatment, obtaining a MOF single-particle layer; finally, adding a proper amount of a polymer binder to fill the gaps between the MOF particles, so that a single-particle layer MOF mixed matrix nanometer porous membrane is obtained. The single-particle layer MOF mixed matrix nanometer porous membrane prepared by the preparation method is composed of a single-particle layer, has an ultrathin thickness and high strength, and the transmembrane transport of substances in the membrane only needs to pass through a single MOF particle, so that the nanometer porous structure of the MOF particle can be used to the maximum extent, the negative influence of polymer blockage and grain boundary between particles on the substance transport of the nanometer porous membrane is avoided, and the single-particle layer MOF mixed matrix nanometer porous membrane has high selectivity and high permeability.

The invention relates to the field of molecular biology and medical genetics, and particularly discloses a method for regulating and controlling transport of a TMC1 plasma membrane through TRAPPC3 and application of the method in an auditory function. The TMC1 is a core protein of a hair cell mechanical electrotransduction (MET) channel, and abnormal positioning of the TMC1 is closely related to hereditary hearing loss. The research finds that the transport protein particle compound component TRAPPC3 can specifically interact with the TMC1 protein to promote the TMC1 to be correctly positioned to a cell membrane from cytoplasm, so that the auditory function of hair cells is maintained. According to the method for regulating and controlling the positioning of the TMC1 from the cytoplasm to the cell membrane through co-expression of the TRAPPC3, a functional TMC1 channel can be reconstructed in a heterologous system, and the method is suitable for deafness molecular mechanism research, drug screening and gene therapy strategy development. According to the invention, the TRAPPC3-TMC1 interaction mechanism is provided for the first time, and a new intervention target is provided for hereditary hearing loss treatment.

The invention discloses a pH-responsive isoGPBG hydrogel and an application of the pH-responsive isoGPBG hydrogel in miRNA delivery, and belongs to the technical field of biopesticides. The pH-responsive isoGPBG hydrogel disclosed by the invention is formed by deprotonating isoguanosine and guanosine under an alkaline condition and cross-linking the deprotonated isoguanosine and guanosine with a boric acid group of PBA to form a pH-sensitive phenylboronic acid ester bond. The hydrogel delivery system can break through plant miRNA transmembrane transport obstacles, and efficient delivery and controllable release of antiviral nucleic acid drugs are achieved. When the hydrogel delivery system is used for delivering miRNA, in-vitro degradation of miRNA can be prevented, and the hydrogel delivery system has an important application prospect in prevention and treatment of plant viral diseases and bacterial diseases.

The application discloses a medicine for preventing and treating plant diseases, a preparation method and application thereof, and belongs to the technical field of biological pesticides. The medicine for preventing and treating plant diseases comprises a hydrogel and nbe-miR159a; and the nucleic acid sequence of the nbe-miR159a is shown as SEQ ID NO:1. The isoGPBG pH-responsive hydrogel is used to coat the nbe-miR159a; not only the transmembrane transport obstacle of plant miRNA is broken through, efficient delivery and controllable release of the antiviral nucleic acid medicine are realized, but also the miRNA can be protected from degradation. The medicine has good inhibiting effect on potato Y virus and Pseudomonas solanacearum, and has important application prospects in the prevention and treatment of plant bacterial diseases and viral diseases.

The present application relates to a kind of twelve urea cage molecules, preparation method and application, belong to chemical synthesis and pharmaceutical preparation technical field.The molecule is non-toxic and neutral, and the urea group in the molecule has multiple hydrogen bond donors and acceptors, it has three-dimensional cavity capable of completely encapsulating substrate, can simulate protein binding site and function.Use organic ammonium sulfate salt as template, obtain twelve urea molecular cage by reacting N bridge-based tripod three urea precursor with diisocyanate or diaminocarbamate.The molecule can be used as a universal tool to chelate different kinds of phosphorus / phosphonate drugs through hydrogen bond, reduce the electronegativity of phosphorus / phosphonate drug, increase the liposolubility of phosphorus / phosphonate drug, improve its membrane transport efficiency.

This invention provides the preparation and application of a class of artificial peptide transporters for promoting transmembrane delivery of peptide drugs. The general structural formula is as follows: The compound involved in this invention consists of three parts: (1) a diamide structure as an anion-binding unit, which binds to the carboxyl anion of the peptide molecule; (2) a crown ether as a cation-binding unit, which binds to the amino cation of the peptide molecule; and (3) a hydrophobic side chain: connecting the anion-binding unit and the cation-binding unit, so that the whole molecule can act as molecular tweezers to form a stable complex with the peptide drug and encapsulate it in a hydrophobic environment to promote its transmembrane transport. The compound obtained in this application has inexpensive raw materials, is easy to synthesize, and significantly improves the bioavailability of peptide drugs. It has shown significant synergistic anticancer activity in both in vitro and in vivo experiments, and therefore has great potential as a pharmaceutical excipient.

Nonaqueous redox flow batteries are one economically promising solution for meeting grid-scale energy storage needs at discharge durations of 10 h or more. However, membrane transport properties in nonaqueous systems are not as well understood as in water. Solvent-specific effects complicate efforts to understand transport in nonaqueous. In one aspect, the disclosure relates to blended cross-linked membranes compositions, methods of making same, and devices, products, and systems comprising same. In one aspect, the disclosed blended cross-linked membranes compositions comprise a cross-linked first polymer and a second polymer. The disclosed blends have desired permeabilities for selected cations and rejected undesired materials from the redox reaction mixture. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

According to the present invention, in the prior art, staphylococcus aureus infection and antibiotic resistance (AMR) are two important factors threatening public health, such that persistent pain and even death may be caused, and the common health can be threatened by the staphylococcus aureus infection and the antibiotic resistance (AMR), such that the common health can be threatened by the staphylococcus aureus infection and the antibiotic resistance (AMR), and the common health can be threatened by the staphylococcus aureus infection and the antibiotic resistance (AMR); therefore, it is urgent to develop alternative antibacterial materials. In the invention, a biocompatible nano system (ZIF-67 HS) based on a hollow spherical zeolite imidazate skeleton is successfully developed, and the biocompatible nano system has a remarkable bactericidal effect on gram-positive staphylococcus aureus in vitro and in vivo. Importantly, the research result shows that S. aureus does not generate secondary drug resistance like traditional antibiotics when being exposed to ZIF-67 HS with sub-minimum inhibitory concentration for a long time; on the contrary, it maintains continuous sensitivity to antibiotic resistant strains. Multi-omics analysis (including transcriptomics, metabonomics and metallomics) is combined with bioassays to prove that ZIF-67 HS destroys a plurality of necessary pathways in S.aures, including amino acid metabolism, iron metabolism, membrane transport, ROS and S.aures infection pathways. According to the invention, a new insight is provided for the potential of nano biological medicines in the aspect of removing bacteria, and a related molecular mechanism is clarified.

The invention provides application of esketamine in preparation of hyperalgesia drugs, and relates to the technical field of clinical medicine. Clinical tests and animal experiments are combined, it is systematically proved that sub-anesthetic dose esketamine can effectively relieve diabetic hyperalgesia, and a novel molecular action mechanism of esketamine is disclosed. Preliminary tests and animal experiments show that the scheme can significantly improve the mechanical pain threshold and reduce the pain score, and the effect is exact. In mechanism, it is clarified for the first time that esketamine regulates and controls downstream PI3K / Akt / GSK-3beta signal channels by inhibiting excessive activation of an NMDA receptor, and abnormal expression and membrane transport of a GLuR1 receptor are reduced, so that central sensitization is reversed. The scheme has the advantages of quick response, no opioid drug dependence, clear mechanism and the like, provides a new safe, effective and clear-target analgesic strategy for clinical treatment of diabetes-related neuropathic pain, and has good transformation and application prospects.

The utility model provides a film cutting and transporting device, wherein the film cutting and transporting device comprises an adhesive film adsorption turntable, an adhesive film transverse cutting mechanism and an adhesive film transporting platform, the adhesive film adsorption turntable is provided with an adhesive film adsorption rotating column with a polygonal cross section, the adhesive film transverse cutting mechanism is provided with a first cutting knife, and the adhesive film transporting platform is provided with a second cutting knife. The first cutting knife can cut off the adhesive film corresponding to the gap position between the two adjacent adsorption faces of the adhesive film adsorption rotating column, and the adhesive film conveying platform is located below the adhesive film adsorption rotating column so as to bear and convey the adhesive film section released by the adsorption face, located on the lower side, of the adhesive film adsorption rotating column. According to the utility model, the phenomenon that the rotary adhesive tape pasting mechanism in the prior art is directly adhered to the surface of the battery piece after the adhesive film section is prepared, so that the battery piece is damaged due to stress concentration can be avoided, the bunching qualification rate of the battery piece is improved, and the quality and the performance of the battery piece are further improved.

The application discloses a coating composition, a coating material and an anti-fouling coating, and the coating composition comprises: pentaerythritol tetra-3-mercapto propionate (PETMP), hexamethylene diisocyanate (HDI), an indole derivative containing a carbon-carbon double bond and an initiator. The indole derivative containing the carbon-carbon double bond is a Friedel-Crafts alkylation product of N-hydroxymethyl acrylamide and the indole derivative. The anti-fouling coating obtained after curing of the coating composition provided by the application has excellent mechanical properties, high anti-biofouling adhesion and strong adhesion between the coating and a substrate. The indole derivative can act on transmembrane transport of calcium ions in cells, trigger Ca 2+ efflux in the intracellular environment, destroy the homeostasis of Ca 2+ in the cells, and have an adverse effect on the growth and development of the cells, thereby inhibiting the adhesion and growth and reproduction of biofouling organisms such as microalgae.

The invention discloses a modified sulfonated polyethersulfone membrane as well as a preparation method and application thereof, and the preparation method of the membrane comprises the following steps: preparing a membrane from a sulfonated polyethersulfone solution by adopting a tape casting method, drying, immersing the formed membrane into an acrylic acid solution, initiating a grafting reaction by ultraviolet light, and then washing and drying to obtain the modified sulfonated polyethersulfone membrane. The carboxyl is grafted to the surface of the membrane through ultraviolet initiation, so that the physical and chemical properties such as hydrophilicity and hydroxyl conductivity of the membrane are remarkably improved, and efficient transmembrane transportation of carriers is realized.

The invention discloses a photo-acid-driven single-hole colloid motor and a preparation method thereof, and belongs to the field of colloid motors. The colloid motor takes a polyelectrolyte single-hole microcapsule loaded with photoacid as a core, and a capsule-like vesicle film is assembled on the surface of the polyelectrolyte single-hole microcapsule. The method comprises the following steps: extracting the thylakoid vesicles embedded with the biomolecular motor FlFo-ATP synthase by virtue of methods of homogenizing and homogenizing, gradient centrifugation and extrusion; 2, preparing a single-hole microcapsule by a heterogeneous nucleation, self-expansion and selective etching method; 3, carrying out layer-by-layer self-assembly by taking the single-hole microcapsule as a template to prepare a polyelectrolyte single-hole microcapsule; and 4, assembling the capsule-like vesicles on the surface of the polyelectrolyte single-hole microcapsule. By utilizing a unique single-hole channel structure, precise regulation and control on light-operated proton release and transmembrane transportation are realized, and the proton driving efficiency is remarkably improved, so that rotation of FlFo-ATP synthase is efficiently activated to synthesize ATP, a light response motion behavior is realized, and directional motion and energy metabolism regulation and control in a complex biological medium can be realized.

The invention discloses an electronic tomato hydroponic device and a cultivation method thereof, and relates to the field of hydroponic cultivation. The control module obtains a preset reference parameter and outputs a control instruction; the direct-current stabilized power supply outputs low-voltage direct current according to the control instruction; the electrode module forms an electric field at the root system of the tomato plant according to the low-voltage direct current and penetrates through a root system-stem pathway to activate a cell membrane ion channel, so that the permeability of a cell membrane of the root system is changed based on electrical stimulation, and transmembrane transportation of anions and cations in a nutrient solution is promoted; the control module also compares the acquired data with a preset reference parameter and outputs an adjustment instruction; the circulation module drives the nutrient solution in the water culture container to flow according to the circulation triggering instruction. The absorption efficiency of the tomato root system on the nutrient solution can be improved, so that efficient and accurate regulation and control of water culture are realized.

This invention discloses a light-driven molecular machine and its application in transmembrane delivery of trivalent metal drugs. The molecular machine features a light-driven molecular motor based on a tetrasubstituted crowded olefin, which consists of a core unit, a polyethylene glycol chain, and trivalent metal ion coordinating groups. This invention utilizes the directional rotational motion of the molecular motor under light irradiation, embedding it into a phospholipid bilayer membrane, and achieving light-controlled transmembrane transport of trivalent metal ions through a "bind-release" mechanism. This molecular machine can selectively complex trivalent metal ions (such as Bi...) 3+ Ga 3+ Ce 3+ (etc.), and accelerates ion transmembrane transport under light-driven conditions, thereby disrupting the intracellular and extracellular ion balance of bacteria. Experiments have shown that this system has significant light-controlled antibacterial activity against Staphylococcus aureus. This invention has advantages such as controllable structure, precise spatiotemporal targeting, and ease of derivatization, and has promising applications in antibacterial therapy and biomedicine.

The invention discloses a method for promoting opening of plant stomata through optogenetics, and belongs to the technical field of plant growth and development research. By activating the light-operated hydrogen ion pump PPR protein, the length and width of the plant stomata can be regulated and controlled, the stomata opening and closing can be controlled, the opening of the plant stomata is promoted, and the stomata conductance of the plant is improved. The light-operated hydrogen ion pump PPR protein is transferred into guard cells, and the length and width of the pores are regulated and controlled under the illumination condition, so that the opening and closing of the pores are controlled. The opening and closing mechanism does not depend on frequent stimulation of external conditions, but accelerates transmembrane transport of hydrogen ions by means of illumination, so that accurate regulation and control of plant stomata can be realized.

The utility model discloses a smoothing mechanism and a diaphragm conveying device, the smoothing mechanism comprises a smoothing assembly and a connecting assembly, the smoothing assembly is movably connected with a first end of the connecting assembly, and a second end of the connecting assembly is used for being connected with a support; when the smoothing mechanism is in the first state, the smoothing assembly is located at the first position, and the smoothing assembly can make contact with a diaphragm and conduct smoothing. And when the smoothing mechanism is in the second state, the smoothing assembly is located at the second position, and the smoothing assembly can be far away from the membrane and avoid the membrane. Therefore, the position of the smoothing assembly can be flexibly adjusted according to working condition requirements. And moreover, due to the arrangement of the avoiding position, the film conveying device can stop smoothing without shutdown, the shutdown time is also reduced, and the production efficiency is conveniently improved.

Disclosed herein are methods of delivering biomolecules to the cytosol of a cell using a substituted tertiary amine (STA) moiety.

The embodiment of the present application belongs to the field of macromolecular substance transmembrane transport, and particularly relates to a cell membrane jet flow perforation device and method and a macromolecular substance transmembrane conduction system. The cell membrane jet flow perforation device comprises a cell introduction flow cavity, a cell capture cavity and a jet flow device. The cell introduction flow cavity has an inlet and an outlet. The cell capture cavity is in communication with the cell introduction flow cavity, and is used for capturing cells entering the cell capture cavity from the inlet. The jet flow device provides jet flow to the cells in the cell capture cavity, so that the cell membranes of the cells in the cell capture cavity form at least one micropore. Compared with the prior art, the perforation device of the present embodiment can perforate the cell membrane in a mechanical manner. When the cells are subjected to the shearing force of the flow field, a plurality of transient micropores can be formed on the cell membranes. In the case of minimizing the damage to the cells, high-throughput direct transmembrane transport of macromolecular substances is realized.

The invention discloses an efficient synthesis method of corynebacterium glutamicum gamma-aminobutyric acid based on membrane transport engineering, and belongs to the technical field of genetic engineering. GABA transporters are screened, firstly, a transporter library is constructed, a series of effective GABA transporters which are genes cg2139, cg2568, cg2339, cg11006 and cg1281 are obtained through screening of a flow cytometry, the transporters obtained through screening are integrated to a genome for fermentation verification, so that corynebacterium glutamicum can produce GABA more efficiently, and the GABA production efficiency is improved. And compared with a control strain FF10, the yield reaches 10.73 g / L and is improved by 34%.

The invention belongs to the technical field of bioengineering, and relates to a recombinant engineering bacterium and application thereof in preparation of mannitol, and the recombinant engineering bacterium is a plasmid-free strain for producing a cofactor NAD < + > by using cheap xylose and nicotinamide. The following genes are knocked out from the strain: a gene ptsG for coding protein EIICBGlc in a glucose PTS system, mtlA for coding EIICA protein in D-mannitol transmembrane transport, a D-mannitol 1-phosphate dehydrogenase gene mtlD in a D-mannitol utilization path, purine de novo synthesis regulation protein purR, and srlAE in a sorbitol utilization gene cluster. The following genes are integrated in a genome of the strain: glucose transport assisting protein glf, glucokinase glcK, xylose utilization gene cluster xylose isomerase xlyA and xylulose phosphorylase xlyB, phosphoribose pyrophosphate synthetase prs, polyphosphate kinase ppk2, nicotinamide phosphoribose transferase nadV and nicotinamide mononucleotide adenylate transferase nadM. The strain is fermented by using glucose and xylose, and provides chassis bacteria for reaction needing cofactor circulation.