98 results about "Membrane transport" patented technology

Compositions and methods have been developed for transporting compounds across membranes with little or no toxicity and, when targeted through the appropriate routes of administration (i.e., lung, gastrointestinal (GI) tract), little or no immune stimulation. The compositions can mediate cellular delivery of compounds that would otherwise not enter cells and enhance the intracellular delivery of compounds that would otherwise enter cells inefficiently. The methods are carried out by contacting a proximal face of a lipid bilayer or membrane (e.g. the surface of an intact cell) with a complex containing a compound (e.g., a therapeutic agent) and a diketopiperazine (DKP). DKP and the compound are non-covalently associated with each other or covalently bound to each other.

Provided is a pharmaceutical composition for permeabilizing fetal membranes including an active ingredient having a log K in the range of 2 to 4, where K is the octanol / water partition coefficient. The active ingredient may be, for example, bupivacaine, sodium lauryl sulfate or oleic acid. Further provided is a system for transfetal membrane transport. The system includes a probe unit adapted for insertion into a female reproductive tract and releasing a substance onto fetal membranes that permeabilizes the membranes. The system is also configured to apply ultrasound radiation to the fetal membranes to further increase the membrane permeability.

Method for on-line prediction of performance of an RO based desalination plant is disclosed. The method includes: (i) a mathematical model of the RO unit; (ii) on-line estimation of membrane physical parameters of the nonlinear mathematical model representing the RO unit; and (iii) analysis of the estimated membrane transport parameter with respect to time. Based on the analysis of these estimated parameters, plant operators can clean the membranes to restore the performance of the RO desalination plant. The method can be implemented in a computer based control system used for data acquisition and control of an RO based desalination plant. The method can help in maintaining the performance of the RO based desalination plants at a desired level and increase membrane life without affecting quality of permeate water produced.

The present invention relates to low-molar mass condensed derivatives of silicic acid of sub-nano particle size characterised by particular structure and specific biological activities. Preparation methods and applications are presented for the here disclosed sub-nano silicic acid (SNSA) which interact with bio-molecules and modify significantly their structure and biological function. Preferred field of application of the inventive silicic acid derivatives is to modulate the structure and biological function of proteins particularly of those involved in reversible phosphorylation within biological signal transduction or membrane transport processes. Structure of the substances, methods for the preparation and stabilization, as well as pharmaceutical compositions comprising the substances and methods of application in the prevention, diagnosis and therapy of diseases are disclosed.

The present invention provides for methods and compositions for transporting agents and macromolecules across biological membranes. In one embodiment, the invention relates to a method for enhancing transport of a selected agent across a biological membrane, wherein a biological membrane is contacted with a composition containing a biologically active rotaxane capable of selectively transporting the selected agent. The host-rotaxane is effective to impart to the agent an amount transport and / or rate of trans-membrane transport across a biological membrane that is greater than the amount and / or rate of trans-membrane transport of the agent without the host-rotaxane.

Isolated nucleic acid molecules, designated MCT nucleic acid molecules, which encode novel MCT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MCT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MCT proteins, mutated MCT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of MCT genes in this organism.

The invention relates to a clamping type residual membrane recovering machine, belonging to the field of agricultural machinery. The machine comprises a traction device, a drive system, a residual membrane box, an earth wheel and a residual membrane recovering device, wherein the residual membrane recovering device consists of a clamping type residual membrane picking wheel, a sprocket rake type residual membrane transporting device, a baseboard, a membrane removing wheel and a contour wheel; the clamping type residual membrane picking wheel consists of a chain wheel, four rows of clamps, a push rod, a clamp bracket, a restoring spring, a baffle block and a cam wheel; the lower end of each clamp is provided with a shifting fork; a plurality of rows of rack bars are arranged on the sprocket rake type residual membrane transporting device; the residual membrane recovering device is arranged at the back side of the residual membrane box in a hinging way; a clamping type residual membranepicking mechanism is arranged at the lower end of the residual membrane recovering device; a contour earth wheel is arranged at the back side of the lower end of the residual membrane recovering device; the baseboard is arranged at the lower side of the sprocket rake type residual membrane transporting device; and the membrane removing wheel is arranged at the upper end of the residual membrane recovering device. The clamping type residual membrane recovering machine is fast in work speed, can be used for recovering small pieces of the residual membrane, is high in residual membrane recovering rate, and can be used for meeting the residual membrane recovering requirement.

The invention relates to the field of biomedicine, in particular to production of a novel human cell-penetrating peptide hPP10 (human Pancreatic Polypeptide) and a transfection method for a mediated plasmid DNA (Deoxyribose Nucleic Acid) of the hPP10. The hPP10 is derived from human cell nucleoproteins; and one segment of polypeptide sequence of a lysine specific demethylated enzyme 4A has a cell penetrating function, can be used for carrying proteins, allows nucleic acids (DNA plasmids) to enter a plurality of types of cells in a transmembrane manner, and is a transmembrane transport carrier with an extremely good development prospect for bioactive molecules such as proteins, nucleic acids and the like.

The invention discloses an on-line detection device and method for heavy metal content in a flue gas, and the device comprises a gas sampler 10, a belt type active filter membrane 80, a filter membrane transport unit 30, an XRF (X-ray fluorescence) detector 40, a wireless communication unit 90 and a control unit; the whole device has the advantages of being simple and ingenious in structure, and easy to popularize; according to the detection method, an X-ray fluorescence spectroscopy method is used for qualitative and quantitative analysis of harmful elements in the flue gas, a burdensome chemical method test is avoided, work efficiency is improved, at the same time the test precision reaches ppm level; by design of the belt type active filter membrane, a simple method for solving the difficult transfer problem between the sampler collection and the detector is provided, and by design of materials of the belt type active filter membrane, enrichment and detection of multiple-state heavy metals can be achieved, and the detection is timely, and high in efficiency.

Methods and compositions for transporting drugs and macromolecules across biological membranes are disclosed. In one embodiment, the invention pertains to a method for enhancing transport of a selected compound across a biological membrane, wherein a biological membrane is contacted with a conjugate containing a biologically active agent that is covalently attached to a transport polymer. In a preferred embodiment, the polymer consists of from 6 to 25 subunits, at least 50% of which contain a guanidino or amidino sidechain moiety. The polymer is effective to impart to the attached agent a rate of trans-membrane transport across a biological membrane that is greater than the rate of trans-membrane transport of the agent in non-conjugated form.

The invention belongs to the field of biomedicine and relates to a human original cell permeable peptide hPP3 and usage thereof. The cell permeable peptide hPP3 provided by the invention has a cell membrane permeation function, thereby being capable of carrying such large molecules as protein to enter a plurality kind of cells in a transmembrane manner; since the peptide segment is derived from human protein, the possibility of causing human body immune response is small, the potential insecurity factors are relatively small, so that the hPP3 can serve as a transmembrane transport carrier having brilliant development prospect of such bioactive molecules as protein, polypeptide, and the hPP3 can be used as an intracellular drug transport carrier.

Photosensitive cardiac rhythm modulation structures and systems are described. A genetically-engineered tissue comprising a population of pacing cells expressing a photosensitive membrane transport mechanism that is responsive to light of a particular wavelength(s) combined with one or more of a light source, a power generator, and a sensor provides pacemaker and / or defibrillator function to a subject. The systems further provide in vitro model systems for electrophysiological studies.

The invention discloses a method for building medicine transport models for research on BCRP (breast cancer resistance proteins) mediation for 3D (three-dimensional) organs of the small intestines of mice. The method includes separating crypts from the small intestines of the mice by means of digestion, suspending the crypts in matrigel and then joining the crypts with cell culture plates and promoting differentiation of the crypts by the aid of ADMEM / F12 media with Respondin-1, m-noggin and m-EGF cell differentiation growth factors to form the 3D organs; carrying out morphologic observation and detecting the expression level of BCRP genes and proteins to verify the feasibility of model theories; carrying out research on the trans-membrane transport activity of BCRP in the 3D organs by the aid of fluorescent substrates Hoechst 33342 of the BCRP and inhibitors Ko143 or YHO-13177 of the fluorescent substrates by co-incubation processes. The method has the advantages that the medicine trans-cell-membrane transport in-vitro models for the research on the BCRP mediation for the 3D organs of the small intestines of the mice are built for the first time, and the method for building the models is easy and convenient to implement and high in detection efficiency and speed and can be widely applied to screening BCRP substrates and inhibitors in an in-vitro manner.

The invention discloses an engineering bacterium for highly expressing a lipopeptide biosurfactant and application thereof, belonging to the fields of biotechnology and biochemical engineering. The engineering bacterium over-expressing transmembrane protein YcxA is constructed by adopting a gene engineering technology, and transmembrane transport of lipopeptide from the inner part of a cell to the outside of the cell is enhanced, so that the yield of the lipopeptide is significantly improved. Compared with an original strain, the obtained gene engineering bacterium over-expressing lipopeptide carrier protein YcxA has the advantages that the yield of surfactin is improved by 97%, the engineering bacterium can be used for producing the lipopeptide biosurfactant and has a good industrial application prospect, and the yield of lipopeptide in a fermentation broth obtained by fermentation is averagely 1-5 g / L.

The present invention relates to 2,4,5-trisulbstituted imidazole compounds and their preparation process and their application in preparing medicines for reversing multiple drug resistance of tumor cell mediated by P-glucoprotein, multiple drug resistance relative protein, lung multiple drug resistance relative protein and other membrane transport proteins and restoring the sensitivity of tumor cells with multiple drug resistance to anticancer medicines.

The invention belongs to the field if biological medical science, and relates to a cell-penetrating peptide hPP8 and application thereof. The provided cell-penetrating peptide hPP8 possesses function of penetrating cell membrane, and is capable of entering into multiple cells in a transmembrane manner while carrying protein and other macro-molecule. Because the peptide comes from human protein, the possibility that the peptide causes human immunity response is small, potential unsafe factors are relatively less, and thus hPP8 is a transmembrane transportation carrier which possesses exploitation prospect and is applicable to protein, polypeptide and other biological active molecules, and is applicable as an intracellular medicine transportation carrier.

Isolated nucleic acid molecules, designated MCT nucleic acid molecules, which encode novel MCT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MCT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MCT proteins, mutated MCT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of MCT genes in this organism.

The invention discloses a method for preparing nanometer magnetic microspheres and an anticancer oral preparation prepared by utilizing the method. The method comprises the process steps of: a, material preparation, in which a carrier material, a framework material, a drug and a solvent are prepared; b, the preparation; c, the stability and drying; and d, the crushing and preparation and so on. The method takes beta-cyclodextrin as the framework material and includes the carrier material (a nanometer magnetic biomaterial) and drug combination and so on. The drug-loaded nanometer magnetic microspheres can quickly reach appointed focus target positions, penetrate target cell membranes to enter cells, realize the trans-membrane transport of target macromolecules, achieve the effects of targeted release and slow / controlled release, improve the drug concentration in target cells, and inhibit the gene expression and amplification of the target cells to ensure that the target cells are degenerated to necrotize or apoptosize; at the same time, the microspheres strengthen the killing activity of T cells and NK cells and the phagotrophic ability of macrophages to the target cells, improve the immunity of organisms, and achieve the effects of multiple treatment and multi-drug resistance reverse.

This invention relates to multi-drug resistance (MDR) in cells, and the use of certain xanthene compounds for determining drug resistance in cells and the effect of test compounds on cell membrane transport by the membrane transporters MDR1, MRP and BCRP. Processes and kits for making these determinations and measuring these effects are described and provided.

The present invention discloses an intelligent printing mechanism for a membrane transport system for large transfusion soft bag production, which comprises a rack, at least one set of thermal transfer printing intelligent marking system that can print large transfusion soft bag lettering, and one set of membrane transport system. The thermal transfer printing intelligent marking system comprises a control panel and at least a printed letter; the membrane transport system is composed of a membrane transport device and a membrane tensioning device. The thermal transfer printing intelligent marking system of the invention can input and print graphics and text, print real-time variables, such as the production date, the batch number, and the validity period, and also can print bar codes; the membrane transport system can make the bag membrane pass under the print head of the intelligent marking system at a normal printing speed required by thermal transfer printing intelligent marking system, so as to solve the problems that the prior art requires batch number replacement for heating, and is low in production efficiency and poor in printing quality, thereby the production efficiency and product quality is improved.

The invention discloses an application of fucoidan in preparation of intestinal prebiotics. The fucoidan has the functions of promoting carbohydrate metabolism, biosynthesis and metabolism of sugar, reducing membrane transport function, enhancing digestive system function or reducing disease infection, promoting production of short-chain fatty acids in intestinal tracts, improving intestinal microbial flora level. The fucoidan is used for preparing intestinal prebiotics, is used for human bodies or livestock and poultry animals, and has a wide application prospect.

The invention discloses a sugar cane sucrose transport protein ShSUT3 and an application of a coding gene thereof. The sugar cane sucrose transport protein ShSUT3 has one of the following amino acid residue sequences: (1) SEQ ID No. 1 amino acid residue sequences contained in a sequence table; (2) the sugar cane sucrose transport protein enables the SEQ ID No. 1 amino acid residue sequences contained in the sequence table to be substituted and / or lacked and / or added through one or more of amino acid residues, has sucrose transport function and is derived from the SEQ ID No. 1. The sugar cane sucrose transport protein ShSUT3 disclosed by the invention has sucrose transport activity, is a cell membrane sucrose transport protein, participates in the transmembrane transport of sucrose and is a key protein which participates in the sucrose accumulating process of a sugar cane. According to the invention, the utilization efficiency and yield of the sucrose can be purposefully enhanced by utilizing the sucrose transport protein gene ShSUT3 disclosed by the invention through gene engineering.

This invention relates to multi-drug resistance (MDR) in cells, and the use of certain xanthene compounds for determining drug resistance in cells and the effect of test compounds on cell membrane transport by the membrane transporters MDR1, MRP and BCRP. Processes and kits for making these determinations and measuring these effects are described and provided.

Membrane translocation peptides, compositions comprising them, chimeric molecules comprising them, and methods of using them to achieve transmembrane transport of various agents.

Method for on-line prediction of performance of an RO based desalination plant is disclosed. The method includes: (i) a mathematical model of the RO unit; (ii) on-line estimation of membrane physical parameters of the nonlinear mathematical model representing the RO unit; and (iii) analysis of the estimated membrane transport parameter with respect to time. Based on the analysis of these estimated parameters, plant operators can clean the membranes to restore the performance of the RO desalination plant. The method can be implemented in a computer based control system used for data acquisition and control of an RO based desalination plant. The method can help in maintaining the performance of the RO based desalination plants at a desired level and increase membrane life without affecting quality of permeate water produced.

According to the invention, two NIRF probes (G1 and G2) with good water solubility are designed. The PEG structure of the probe improves the hydrophilicity of the probe, and an in vitro spectrum and alipid-water distribution coefficient prove that G1 and G2 have a relatively low aggregation trend. The biotin structure enhances the targeting of the probe to BR positive tumor cells, and G1 and G2 show good fluorescence imaging effects in in-vitro cell experiments and in-vivo fluorescence imaging. Due to the cooperation of a specially selected hydrophilic group-PEG structure and targeting group-biotin, the probe G1 and the probe G2 can be balanced between hydrophilicity and targeting property, so that balance is achieved in multiple aspects such as fluorescence intensity, tumor targeting property, transmembrane transport property, residence time in tumor cells, and the prepared probes G1 and G2 have the advantages of high fluorescence intensity, strong targeting, strong transmembrane transportation and long residence time in tumor cells.