Production of cell-penetrating peptide hPP10 (human Pancreatic Polypeptide) and transfection method for mediated plasmid DNA (Deoxyribose Nucleic Acid) of hPP10

Abstract

The invention relates to the field of biomedicine, in particular to production of a novel human cell-penetrating peptide hPP10 (human Pancreatic Polypeptide) and a transfection method for a mediated plasmid DNA (Deoxyribose Nucleic Acid) of the hPP10. The hPP10 is derived from human cell nucleoproteins; and one segment of polypeptide sequence of a lysine specific demethylated enzyme 4A has a cell penetrating function, can be used for carrying proteins, allows nucleic acids (DNA plasmids) to enter a plurality of types of cells in a transmembrane manner, and is a transmembrane transport carrier with an extremely good development prospect for bioactive molecules such as proteins, nucleic acids and the like.

Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to the production of a novel human-derived cell-penetrating peptide hPP10 and a method for mediating plasmid DNA transfection. Background technique

[0002] With the completion of the Human Genome Project and the rise of proteomics, more and more biomolecules such as proteins, polypeptides, and nucleic acids are found to be likely to become therapeutic drugs. However, unlike traditional drugs, these therapeutic molecules have low stability in vivo, and molecules that need to function in cells are difficult to enter cells, which limits their promotion and application as drugs. Therefore, it is an urgent problem to develop an economical and safe carrier system that can effectively carry these therapeutic biomacromolecules into target cells.

[0003] In recent years, non-viral drug delivery vehicles have been placed with high hopes due to their safety, low toxicity, and low immune response....

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