353 results about "Transmembrane protein" patented technology

The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

A method for screening compounds for their ability to interact with transmembrane proteins is provided. Also provided is a method for determining whether proteins such as transmembrane proteins are able to oligomerize.

The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.

Disclosed are novel water membranes comprising lipid bilayers incorporating functional aqua-porins. The lipid bilayers are arranged in sandwich structures including hydrophilic or hydro-phobic support materials. Also disclosed are water purification devices / systems, including reverse osmosis filtering devices that include membranes having functional aquaporins. Methods of water purification and methods of preparing the membranes are also disclosed. Further, the invention provides for a new type of perforated, hydrophobic polymer film and to membranes containing lipid bilayers having other transmembrane proteins than aquaporins introduced.

Methods and compositions are provided for detecting changes in membrane potential in membranes biological systems. In one aspect, the method comprises;a) providing a living cell with a first reagent comprising a charged hydrophobic molecule which is typically a fluorescence resonance energy transfer (FRET) acceptor or donor, or is a quencher and is capable of redistributing within the membrane of a biological membrane in response to changes in the potential across the membrane;b) providing the cell with a second reagent that can label the first face or the second face of a biological membrane within the cell;c) detecting light emission from the first reagent or the second reagent.One aspect of this method involves monitoring membrane potential changes in subcellular organelle membranes in a living cells.Another aspect of the invention is the use of certain embodiments of the method for the screening of test chemicals for activity to modulate the activity of a target ion channel.Another aspect of the present invention is a transgenic organism comprising a first reagent that comprises a charged hydrophobic fluorescent molecule, and a second reagent comprising a bioluminescent or naturally fluorescent protein.