53results about How to "Simple and rapid method" patented technology

The present invention relates to the field of a light emitting device (1), comprising a light emitting diode (2) arranged on a submount (3), said device having a lateral circumference surface (6) and a top surface (8), and an optically active coating layer (7), said coating layer (7):covering along at least a part of said circumference surface (6),extending from the submount (3) to said top surface (8), andessentially not covering the top surface (8). A method for producing the device is also disclosed.

A solid material is treated, the chemical structure of which defines reactive protogenic hydrophillic functions accessible to gases, by applying at least one gas stream (3) onto at least one microdispersion (5) of at least one grafting reagent RX produced on the solid material, R being a hydrophobic group, X being chosen so that HX is volatile under normal conditions, R and X being chosen so that the reaction of RX on the hydrophillic functions produces covalent grafting of the hydrophobic group R with formation of the compound HX, it being possible for the reaction to be carried out in a solid / gas heterogeneous medium on all the reactive hydrophillic functions accessible to gases and only on these. The invention extends to the hydrophobic solid material obtained, and is applicable to the obtaining of natural or artificial fibrous or inorganic structures impermeable to water and to aqueous solutions and / or absorbing fats.

The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

The invention relates to a method for determining an analyte by means of binding reactions, which method comprises: i) applying the sample to an application zone for sample (ASZ) on a flow matrix in which transport of components present in the sample can take place (transport flow), the flow matrix further exhibiting: a) optionally an application zone (AR*Z) for a binding reactant (Reactant*=R*) which is analytically detectable; b) a detection zone (DZ), which is located downstream of ASZ and exhibits an additional binding reactant (Capturer) firmly anchored to the matrix, and in which a complex (signal complex) containing the Capturer and the analyte and / or Reactant* is formed during the reaction, and ii) detecting the signal complex in the detection zone, the measured signal being used for determining the analyte. According to the invention, the flow matrix comprises at least one separation zone (SZ) between ASZ and DZ, which zone exhibits a structure (ligand) having binding capability for a component that is transported in the matrix and which would affect the measurable signal if the component is transported into DZ. The invention also relates to a test kit comprising the flow matrix.

The present invention describes an extremely effective, simple, novel, inexpensive, safe and quick method to treat both acne and warts, each with a different etiology and different clinical symptoms, by topically applying an effective amount of one or more polyvalent metal compounds. The polyvalent metal compounds include, but are not limited to, bismuth compounds, zinc compounds, magnesium compounds, aluminum compounds, calcium compounds, copper compounds, titanium compounds, manganese compounds, chromium compounds, barium compounds and iron compounds. The present invention can also be applied to the treatment of rosacea. The present invention can also be employed to prevent scarring and to facilitate healing or elimination of the scars once formed.

A window protection structure for a building including novel structure for affixing a sheet of protection material over the window opening. The sheet material is cut to the size of the window opening in the building, and holes are formed therein. U-shaped clips are slid onto an upper edge of the sheet material, and a plurality of brackets are provided with a pair of holes formed therein. Cords loops are secured through the holes in the brackets. The brackets are adapted for receipt by the interior of the window and the cords are threaded to the exterior of the window. The cord loops are threaded through the holes in the sheet material. A bungee cord is then used for securing the ends of the cord loops, whereby the sheet material is held firmly against the window.

A method of separating and purifying nucleic acids from samples containing cells, such as blood and culture solutions. According to the method of the invention, a cell extract obtained by cell disruption is adsorbed by a filter made of a nonwoven fabric and the nucleic acid is eluted after washing the filter. Alkaline conditions of pH 12 or higher may be employed for elution of the nucleic acid, or the filter-adsorbed nucleic acid may be eluted by treatment with active oxygen or by using a surfactant. Nucleic acids separated and purified by the method of the invention can be used in nucleic acid amplification and nucleic acid sequence analysis techniques.

The present invention relates to the field of a light emitting device (1), comprising a light emitting diode (2) arranged on a submount (3), said device having a lateral circumference surface (6) and a top surface (8), and an optically active coating layer (7), said coating layer (7):covering along at least a part of said circumference surface (6),extending from the submount (3) to said top surface (8), andessentially not covering the top surface (8). A method for producing the device is also disclosed.

A process for the preparation of liposomes is described. The process includes the steps of formation of a lipid film on a ceramic filter, preferably an asymmetric ceramic filter, followed by hydration of the lipid film obtained. The liposomes thus formed are recovered. Multilamellar liposomes prepared by the process are also described.

Eluting reagents and methods for isolating DNA from biological materials are provided.

Processes for isolating, amplifying, and characterizing DNA from biological materials are provided. DNA is isolated by contacting a biological material on a solid support which is preferably pre-treated with a lysing reagent. The isolation process is simple and efficient and provides a source of purified DNA without the use of harmful organic solvents such as urea and guanidine-based solvents. The purified DNA and remaining fractions of biological material may be characterized or amplified as necessary.

The present invention describes an extremely effective, simple, novel, inexpensive, safe and quick method to treat both acne and warts, each with a different etiology and different clinical symptoms, by topically applying an effective amount of one or more polyvalent metal compounds. The polyvalent metal compounds include, but are not limited to, bismuth compounds, zinc compounds, magnesium compounds, aluminum compounds, calcium compounds, copper compounds, titanium compounds, manganese compounds, chromium compounds, barium compounds and iron compounds. The present invention can also be applied to the treatment of rosacea. The present invention can also be employed to prevent scarring and to facilitate healing or elimination of the scars once formed.

The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.

This invention relates to a composition, kit, DNA chip, and use thereof for detecting, diagnosing, and predicting metastasis of kidney cancer and / or for predicting the prognosis for kidney cancer, comprising one or a plurality of polynucleotides selected from the group consisting of polynucleotides, mutants thereof or fragments thereof, the expression levels of which vary in kidney cancer cells from a patient with a poor prognosis when compared with that in kidney cancer cells from a patient with a good prognosis; or antibodies or fragments thereof that bind specifically to polypeptides, mutants thereof or fragments thereof, the expression levels of which vary in the similar manner.

An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified using oligonucleotide primers and DNA polymerase. The present invention provides a nucleic acid amplification method which comprises performing incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment, wherein a tag sequence is added at the 5′ end of the first oligonucleotide primer, and the tag sequence is a nucleotide sequence on the template nucleic acid fragment which is present downstream of the sequence which is substantially complementary with the 3′ end region of the first oligonucleotide primer (a region where the first oligonucleotide is annealed to the template nucleic acid).

The general field of the invention includes methods of calibrating X-ray detection systems, the systems including at least one X-ray generator and a detection array having a matrix of detecting semiconductor pixels and processing and calibration electronics. The calibration method includes, for all or some of the pixels: operating the X-ray generator at its nominal high voltage, the generator being placed opposite the detector; counting, using the processing and calibration electronics, the pulses emitted by each pixel through the effect of the radiation produced by the generator; establishing, for each pixel, an amplitude distribution for the counted pulses; applying, to each amplitude distribution, a statistical indicator so as to identify a particular amplitude, this particular amplitude then corresponding to the energy corresponding to said statistical indicator; and adjusting, using the processing and calibration electronics, calibration parameters for each pixel, taking account of the energy-amplitude relationship thus established.

A window protection structure for a building including novel structure for affixing a sheet of protection material over the window opening. The sheet material is cut to the size of the window opening in the building, and holes are formed therein. U-shaped clips are slid onto an upper edge of the sheet material, and a plurality of brackets are provided with a pair of holes formed therein. Cords loops are secured through the holes in the brackets. The brackets are adapted for receipt by the interior of the window and the cords are threaded to the exterior of the window. The cord loops are threaded through the holes in the sheet material. A bungee cord is then used for securing the ends of the cord loops, whereby the sheet material is held firmly against the window.

In the method, a tool is tested for an aligning fit or an imbalance in a tool spindle. The tool spindle is set into rotation. A light beam is used to check whether the tool wobbles. The tool spindle and the light beam are moved relative to one another until the light beam impinges on the area covered by the wobbling movement and a received signal generated from the light beam has an intermittent signal path for the first time. The then prevailing relative position between the tool spindle and the light beam is evaluated in respect of a non-aligning fit of the tool or in respect of a tool imbalance.

In the method, a tool is tested for an aligning fit or an imbalance in a tool spindle. The tool spindle is set into rotation. A light beam is used to check whether the tool wobbles. The tool spindle and the light beam are moved relative to one another until the light beam impinges on the area covered by the wobbling movement and a received signal generated from the light beam has an intermittent signal path for the first time. The then prevailing relative position between the tool spindle and the light beam is evaluated in respect of a non-aligning fit of the tool or in respect of a tool imbalance.

The present invention relates to a method capable of detecting proliferative bacteria in food and beverage, particularly acid or neutral beverage at low cost within a short time, a detection kit which enables simple measurement, and a technology for examination of harmful bacteria in food and beverage with respect to a method for rapid determination of proliferation properties of bacteria capable of producing guaiacol in food and beverage. There is disclosed a method for detecting bacteria capable of producing guaiacol, which comprises culturing or incubating a culture solution containing a specimen for a predetermined time in the presence of vanillic acid, and measuring the produced guaiacol by qualitative or quantitative analysis. Also there is disclosed a kit for detection of bacteria capable of producing guaiacol, comprising a container, and an acid or neutral culture medium containing a vanillic acid substrate for production of guaiacol, which is contained in the container and also sterilized and sealed. Furthermore there is disclosed a method for rapid determination of proliferation properties of bacteria capable of producing guaiacol in food and beverage, which comprises incubating a sporophyte of spore-forming bacteria capable of producing guaiacol for a predetermined time in the food and beverage in the presence of vanillic acid, and measuring the produced guaiacol by qualitative or quantitative analysis.

The present invention provides a bacteria detection device, bacteria detection method and bacteria detection kit enabling rapid and accurate determination of bacteria present in a test sample, and particularly spore-forming, aerobic bacteria, with a simple procedure. The bacteria detection device according to the present invention is a microarray type of device in which an oligonucleotide, which is based on a nucleotide sequence specific to a genus or species to which a target spore-forming, aerobic bacteria belongs, is immobilized on a substrate. Spore-forming, aerobic bacteria present in a test sample can be easily, rapidly and accurately detected and identified based on the presence or absence of successful hybridization between a probe prepared from the test sample and the oligonucleotide immobilized on the substrate.

A composite part including a fibrous assembly including at least two preforms each of which includes layers of fibrous cloths. Each preform includes at least one opening or protruding structure which is substantially complementary to the structure of the other preform. The first and second preforms are engagement with one another and are firmly connected by a polymer matrix being injected around the preforms.

This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method.

The invention relates to a method for preparing a lithium and vanadium oxide and the thus obtained products. The method comprises preparing a precursor gel by reacting hydrogen peroxide with V2O5-α in an aqueous medium in the presence of a lithium precurosor and exposing the gel to a heat treatment in an oxidant atmosphere at a temperature ranging from 260° C. to 580° C. A compound of a formula Li1+αV3O8, (0.1<α<0.25) comprises needle-shaped grains having a bimodal distibution, wherein the length (L) of the first distribution needles ranges from 10 to 50 μm and the length (L) of the second distribution needles ranges from 10 to 10 μm.

A method for the detection and / or quantification of at least one target nucleic acid or target polypeptide in a sample of nucleic acids or polypeptides comprising the steps of: a) providing a sample comprising nucleic acids or polypeptides; b) labeling the nucleic acids or polypeptides with a ligand conjugate, the ligand conjugate comprising a first element binding to the nucleic acids or polypeptides and a second element which is a capture ligand; c) contacting the nucleic acid-ligand conjugates or polypeptide-ligand conjugates with at least one capture probe, the capture probe hybridising with or binding to at least one target nucleic acid or target polypeptide; d) adding i) an oxidoreductase enzyme, wherein the oxidoreductase enzyme is recognised by the capture ligand, or ii) a complex comprising an oxidoreductase enzyme bound to a capture receptor, the capture receptor capable of binding to the capture ligand; e) adding a redox polymer, the redox polymer binding to the oxidoreductase enzyme, thereby resulting in the transfer of electrons from the enzyme via the redox polymer to an electrode surface; and f) detecting and / or quantifying the presence of the target nucleic acid(s) or target polypeptide(s).

A method for the detection and / or quantification of at least one target nucleic acid or target polypeptide in a sample of nucleic acids or polypeptides comprising the steps of: a) providing a sample comprising nucleic acids or polypeptides; b) labeling the nucleic acids or polypeptides with a ligand conjugate, the ligand conjugate comprising a first element binding to the nucleic acids or polypeptides and a second element which is a capture ligand; c) contacting the nucleic acid-ligand conjugates or polypeptide-ligand conjugates with at least one capture probe, the capture probe hybridizing with or binding to at least one target nucleic acid or target polypeptide; d) adding i) an oxidoreductase enzyme, wherein the oxidoreductase enzyme is recognized by the capture ligand, or ii) a complex comprising an oxidoreductase enzyme bound to a capture receptor, the capture receptor capable of binding to the capture ligand; e) adding a redox polymer, the redox polymer binding to the oxidoreductase enzyme, thereby resulting in the transfer of electrons from the enzyme via the redox polymer to an electrode surface; and f) detecting and / or quantifying the presence of the target nucleic acid(s) or target polypeptide(s).

A method for determining an optimal voltage pulse for programming a flash memory cell, the optimal voltage pulse being defined by a voltage ramp from a non-zero initial voltage level during a programming duration, wherein the method takes into account a set of parameters including a programming window target value and a drain current target value of the memory cell.