12 results about "Enzyme binding" patented technology

The application discloses a PROTAC compound with RORgamma t receptor targeted degradation and purposes thereof, which is a compound with a structure shown in a general formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutical composition thereof. e is a ligand capable of combining with an E3 ubiquitin ligase; the L is a linking group covalently combining at least one R e and at least one R W ; the R w is a target protein RORgamma t binding ligand; the application is based on the target point of RORgamma t and the PROTAC technology, and a series of RORgamma t-PROTACs are designed and synthesized for the first time. The mechanism is that the target protein ligand and the E3 ubiquitin ligase ligand are combined with POI and E3 ligase respectively, so as to form a ternary complex of "POI-PROTAC-E3 ligase". Then, the POI is marked with a ubiquitination label, and is degraded by a proteasome. The advantages of RORgamma t-PROTACs mainly include targeting of "undruggable proteins", small dosage, low toxicity, and difficulty in drug resistance. W -L-R e (I)

This invention relates to the field of biopharmaceutical technology, and more particularly to a type of beeswax ( Fraxinus pennsylvanica This invention relates to a pollen allergen composition, its preparation method, and its application. It reveals that Chinese ash pollen contains more different allergenic proteins compared to European and American ash pollen. Therefore, using Chinese ash pollen as raw material, the resulting ash pollen allergen composition contains novel allergenic protein components that bind more strongly to the serum of Chinese ash pollen allergy patients, resulting in superior efficacy. This invention utilizes the ash pollen allergen composition to prepare a kit. This kit is highly sensitive, highly specific, and easy to operate. By changing the enzyme conjugate, it can be used for two purposes in one kit, achieving dual functions: detection of ash pollen allergen-specific IgE antibodies (in vitro allergen diagnosis) and quantitative detection of IgG antibodies (evaluation of desensitization therapy efficacy) in allergy patients.

This application relates to a source-aware activity ranking-based method for screening DPP-IV repressive peptides, comprising: constructing a training dataset containing positive samples and at least three levels of negative samples; extracting multimodal features of peptides, including at least sequence deep semantic embedding features and pocket structure features; explicitly aligning the above features using a cross-attention mechanism to generate fusion features; inputting the fusion features into the classification branch and ranking branch of a decoupled prediction architecture, outputting classification probability and activity ranking score respectively, wherein the ranking branch uses an independent ranking model and is trained based on preference pairs constructed from data within the same assay source; and performing virtual screening output by combining classification probability, activity ranking score, multidimensional confidence judgment, and ADMET safety screening results. This application achieves structurally interpretable characterization of substrate-enzyme binding modes and improves the statistical significance and generalization stability of peptide activity ranking under cross-laboratory batch effects.

Plasma kallikrein binding proteins and methods of using such proteins are described.

We describe compositions of DNA donor molecules compatible with various gene insertion methods, offering key advantages in delivery, nuclear localization, and immune evasion. The modified DNA donor molecules are predominantly single-stranded but include short regions of double-stranded DNA to reconstruct enzyme binding sites, and can be combined with other methods to enhance donor recruitment to the cell nucleus.

The application discloses a method for detecting escherichia coli in food by Fe3O4@Mo-CDs nano-enzyme combined with aptamer, wherein the near-infrared light responsive molybdenum doped carbon dot modified Fe3O4@Mo-CDs nano-enzyme is synthesized by a hydrothermal method, the nano-enzyme has excellent NIR enhanced peroxidase-like activity and magnetism, oxidizes 3,3',5,5'-tetramethylbenzidine to generate blue oxidized TMB, after the surface of the nano-enzyme is functionalized by escherichia coli nucleic acid aptamer, the peroxidase-like activity of the Fe3O4@Mo-CDs nano-enzyme is inhibited, when escherichia coli exists, the nano-enzyme can be selectively combined with the escherichia coli E. coli , and further inhibits the peroxidase-like activity of the nano-enzyme, when the nano-enzyme is used for detecting escherichia coli in juice, cake and other samples E. coli , the detection limit is as low as 0.767 CFU / mL, the nano-enzyme has higher reliability and accuracy, and the application realizes magnetic target separation and visual detection of escherichia coli E. coli .

The application belongs to the technical field of nano-enzyme sensing, and particularly relates to a three-mode phytic acid detection method based on a double-enzyme cascade reaction and application thereof. The detection method uses CAU-10-NH2 metal organic framework as a light-responsive nano-enzyme, combines the light catalytic oxidase activity of ALP and CAU-10-NH2, acquires signals through three modes of fluorescence spectrum, ultraviolet-visible absorption spectrum and photothermal imaging, and quantitatively analyzes the phytic acid content according to the signal change. Phytic acid inhibits the ALP activity, controls the generation amount of ascorbic acid, further influences the oxTMB content generated by CAU-10-NH2 catalyzing TMB oxidation, and realizes the synchronous change of fluorescence, colorimetric and photothermal signals. The application first discovers that CAU-10-NH2 has light catalytic oxidase activity, and only the -NH2 substituted derivative in the CAU-10 series (including CAU-10-H, -OH, -Br, -NO2, etc.) exhibits the activity, which expands the application of aluminum-based MOFs in the field of nano-enzyme sensing.

Plasma kallikrein binding proteins and methods of using such proteins are described.

This invention provides a nanozyme, its preparation method, and its application, belonging to the field of analytical chemistry detection technology. The nanozyme is vanadium oxide doped with a metal, in the form of nanoribbons. The metal is titanium, which is atomically loaded onto the vanadium oxide, with an atomic percentage of 1%-2%. The nanozyme of this invention possesses dual-enzyme activity similar to NADH oxidase and peroxidase. Utilizing its dual-enzyme activity in combination with natural enzymes, the amount of hydrogen peroxide is converted into a final measurement standard. Through the color change of a 3,3',5,5'-tetramethylbenzidine solution system, rapid qualitative and quantitative analysis of biomarkers for rare genetic metabolic diseases is achieved, providing a rapid, sensitive, and reliable colorimetric detection method.

This invention discloses a flexible intelligent immobilization carrier with molecular chaperone-like function and its applications. The flexible polymer immobilization carrier obtained by this invention using a high-throughput screening method exhibits high affinity and good selectivity for enzyme proteins, and can replace traditional immobilization carriers. This immobilization carrier is a flexible polymer prepared by chemical methods, possessing high stability, long service life, and strong resistance to harsh environments, overcoming the shortcomings of traditional immobilized enzyme carriers such as poor selectivity for enzyme proteins, high preparation costs, and reduced enzyme catalytic activity. The immobilization carrier prepared by this invention is a flexible polymer, which is beneficial for adapting to the flexible conformation of enzymes, enabling it to perform molecular chaperone functions, bind to denatured and inactivated enzymes, and utilize the specific molecularly imprinted holes of the flexible polymer to restore the structure and catalytic performance of enzymes without the need for external conditions and complex experimental equipment, thus achieving the refolding of denatured enzymes.