212 results about "Enzyme binding" patented technology

The description relates to cereblon E3 ligase binding compounds, including bifunctional compounds comprising the same, which find utility as modulators of targeted ubiquitination, especially inhibitors of a variety of polypeptides and other proteins which are degraded and/or otherwise inhibited by bifunctional compounds according to the present disclosure. In particular, the description provides compounds, which contain on one end a ligand which binds to the cereblon E3 ubiquitin ligase and on the other end a moiety which binds a target protein such that the target protein is placed in proximity to the ubiquitin ligase to effect degradation (and inhibition) of that protein. Compounds can be synthesized that exhibit a broad range of pharmacological activities consistent with the degradation/inhibition of targeted polypeptides of nearly any type.

The description relates to cereblon E3 ligase binding compounds, including bifunctional compounds comprising the same, which find utility as modulators of targeted ubiquitination, especially inhibitors of a variety of polypeptides and other proteins which are degraded and/or otherwise inhibited by bifunctional compounds according to the present disclosure. In particular, the description provides compounds, which contain on one end a ligand which binds to the cereblon E3 ubiquitin ligase and on the other end a moiety which binds a target protein such that the target protein is placed in proximity to the ubiquitin ligase to effect degradation (and inhibition) of that protein. Compounds can be synthesized that exhibit a broad range of pharmacological activities consistent with the degradation/inhibition of targeted polypeptides of nearly any type.

This invention relates to novel quinoline-based divalent metal ion chelating ligands of Formula I,

wherein A or B are independently —CR⁷R⁸, or —CH(R⁹)CH(R¹⁰). X is hydrogen, C₁-C₁₀ alkyl; —OH, or —NR¹¹R¹². R¹ to R¹² are various substituents selected to optimize the physicochemical and biological properties such as enzyme binding, tissue penetration, lipophilicity, toxicity, bioavailability, and pharmacokinetics of compounds of Formula 13. R¹ to R¹² may include, but are not limited to hydrogen, alkyl, acyl, hydroxyl, hydroxyalkyl, substituted or unsubstituted aryl, amino, aminoalkyl, alkoxyl, aryloxyl, carboxyl, halogen, alkoxycarbonyl, cyano, and other suitable electron donating or electron withdrawing groups. The compounds of the present invention are useful for inhibiting the activity of viral enzymes responsible for the proliferation of human immunodeficiency virus (HIV).

The invention provides a method for modifying the surface of a solid material (e.g. a polymeric matrix). The method is versatile and can be used to prepare polymeric matrices having altered, improved, or specifically engineered properties. Additionally, the method can be used to prepare polymeric matrices that have reactive groups that can be used to immobilize upon the matrices a variety of other “ligand” groups, e.g. a bio-selective affinity group, a chromophore, a dye, an amphiphile, a chiral group, a peptide, a protein, an antibody, an amino acid, an ion exchange group, a detectable group, a carbohydrate, a nucleic acid, a catalyst, a substrate for enzyme binding thereto, an enzyme inhibitor or enzyme co-factor for enzyme binding, or the like.

The invention discloses a luteinizing hormone nano-magnetic particle chemiluminescence quantitative immunoassay kit. The kit comprises a luteinizing hormone calibration product, a nano-magnetic particle suspension coupled with streptavidin, a biotin-marked luteinizing hormone antibody, a luteinizing hormone antibody enzyme binding substance, a luteinizing hormone quality control product, a chemiluminescence solution A and a chemiluminescence solution B, a 20-times concentrated washing solution and a reaction tube, wherein the used enzyme is horseradish peroxidase, the purity RZ of the horseradish peroxidase is not less than 3.0, and the activity is not less than 250U/mL. In addition, the invention further discloses a preparation method of the kit. Compared with the existing kit, the kit disclosed by the invention has the advantages of high sensitivity, a wide range of measurable concentrations, long effective period of a reagent, high degree of automation in detection and the like, and is simple to operate.

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

The invention discloses a sheep echinococcosis ELISA (enzyme-linked immunosorbent assay) antibody detection kit. The detection kit comprises a sheep echinococcosis recombinant antigen Eg95 pre-coated ELISA plate, a sample diluent, a positive reference solution, a negative reference solution, an enzyme bonder, a scrubbing solution, a substrate color-developing solution and a stop solution. A preparation method of a sheep echinococcosis recombinant antigen Eg95 comprises the steps as follows: firstly, PCR (polymerase chain reaction) amplification is performed on an Eg95 gene segment, then EcoRI and XhoI are used for performing enzyme digestion on a PCR product and a prokaryotic expression vector pET-32a simultaneously, a target segment and the vector after gel recovery are connected to construct a recombinant plasmid pET-EG95, and the recombinant plasmid is transformed into Escherichia coli, induced by IPTG (isopropyl-beta-d-thiogalactoside) for expression and purified by Ni-NTA. According to the kit, recombinant protein Eg95 is used as the antigen to detect a sheep echinococcosis antibody, and the antibody detection kit is high in specificity, high in sensitivity and good in stability.

The invention relates to a kit for quickly detecting a swine fever antibody. In the method, a pair of specific primers is designed, a relatively conservative gene sequence is cloned, an antigen aiming at a swine fever E2 protein is expressed through a pronucleus expression technology, and, on the basis, the kit containing an enzyme linked plate coated with a high-purity and high activity swine fever virus specificity antigen, an enzyme conjugate of rabbit-anti-swine monoclonal antibody containing an HRP (Horseradish Peroxidase) marker, a TMB (Tetramethylbenzidine) color developing liquid and the like is prepared. The kit can quickly detect the swine fever antibody in blood serum or blood plasma and has strong specificity and high sensitivity.

The disclosed dual-sandwich enzyme-linked immunologic diagnosis agent box for hepatitis-B core antibody comprises: a micro-porous reaction plate composed by the hepatitis-B core antigen recombinant with purity more than 90% and 5*104~5*105u/mg titer, and the enzyme compound composed by the hepatitis-B core antigen recombinant marked by HRP. This invention has high specificity and sensitivity and convenient to qualitative or semi-qualitative detection.

The invention discloses an identification method and an application of an insulin mass spectrum peptide graph. According to the method, a V8 enzyme is adopted, and the V8 enzyme is combined with lysylendopeptidase, specific hydrolysis of insulin is carried out, and a peptide graph containing insulin characteristic fragments is established through a liquid chromatograph-mass spectrometer. The method is characterized in that a buffer solution is used for providing an enzymolysis environment with the pH value of 7.5-9.5, wherein a substrate and the enzyme are reacted overnight according to the mass ratio of insulin to V8 enzyme to lysyl endopeptidase of (100-1000) to (100-500) to 1, the reaction liquid is diluted for 10-30 times, a liquid phase separation mass spectrometry detector is adopted for detection, and a characteristic peptide graphs of a variety of insulin are established. By adopting the mass spectrum peptide graph, various different kinds of insulin can be accurately determined, and the method is accurate and reliable.

The present invention provides targeted enzymes that bind to targets better than the corresponding pre-targeted enzymes bind the target under like conditions, methods of making targeted enzymes, methods of using targeted enzymes to treat diseases, and pharmaceutical compositions comprising targeted enzymes.

The invention discloses a method for fast screening a topoisomerase I inhibitor from a natural product, and relates to the analysis field of natural products. The method comprises the following steps of: (1) preparation of an enzyme binding reaction buffer solution; (2) preparation of a to be detected sample; (3) preparation of a standard solution; (4) binding reaction of the to-be-detected sample and normal and inactive topoisomerase I; (5) chromatography-mass spectrometry detection analysis of reaction sample; and (6) enrichment ratio of the topoisomerase I to the inhibitor. The ultrafiltration membrane technology and the chromatography-mass spectrometry united technology united method are applied to the screening of the topoisomerase I inhibitor, the sample analysis speed is fast and the specificity is strong so as to conveniently recognize a medicine ligand combined with a biological target molecule; meanwhile, the sample analysis dosage is less, and the spectrogram information quantity is large so as to realize the fast screening of the lead compound, the method has great advantage on the aspect of researching mutual effect of medicine small molecule ligand and biological macromolecule receptor; the method is suitable for analyzing in-vitro enrichment ratio of the natural product extract or monomer compound to the topoisomerase I inhibitor.

The invention provides a Chinese herbal medicine complex enzyme detergent as well as a preparation method and application thereof. Raw materials of the Chinese herbal medicine complex enzyme detergent include a Chinese herbal medicine composition and a complex enzyme composition. According to the invention, Chinese herbal medicines specially owned by China are combined with enzyme, the capability of removing bloodstains and spots of enzyme is utilized, the Chinese herbal medicines with the characteristic of inhibiting Salmonella, colibacillus and the like are screened, and then the enzyme and the Chinese herbal medicine are compounded, so that the guarantee period of eco-eggs is prolonged under the synergistic effect of the enzyme and the Chinese herbal medicines; besides, the bloodstains and spots can be resolved completely, so that the soil removal efficiency can be improved.

The invention discloses enzyme-lectin conjugate nano-particles and a preparing method thereof. According to the preparing method, raw materials for preparing the enzyme-lectin conjugate nano-particles include enzyme, lectin and a cross-linking agent, as well as magnetic nano-particles and/or a reductant. The enzyme-lectin conjugate nano-particles can be prepared from, by weight, 10 parts of enzyme, 50-500 parts of lectin, 0.1-300 parts of cross-linking agent and 0-1 parts of reductant. The enzyme-lectin conjugate nano-particles can also be prepared from, by weight, 10 parts of enzyme, 50-500 parts of lectin, 1-100 parts of magnetic nano-particles, 0.1-300 parts of cross-linking agent and 0-1 parts of reductant. The enzyme-lectin conjugate nano-particles are dispersed in aqueous solution at a conventional catalytic reaction temperature and have high catalytic activity. Lectin combined with enzyme can attract saccharides substrates, so that the aqueous-phase activity of the conjugate can be further improved. Enzyme molecules in enzyme-lectin-magnetic nano-particle conjugate nano-particles are adsorbed by lectin, covalent cross-linking occurs between enzyme molecules and lectin and magnetic nano-particles, and therefore recoverability of the catalyst is realized based on the realization of high catalytic activity.