13 results about "Pollen allergen" patented technology

Tree pollen allergy is seasonal allergy caused by various tree pollen spread in air. The allergy affects a substantial proportion of populations, is particularly significant in European and North America areas, and is characterized by symptoms such as sneezing, nasal obstruction, rhinorrhea, eye itching and lacrimation, and throat irritation. The symptoms typically occur in spring, at which birch, alder, hazel and oak release pollen into the air. Tree pollen allergy is a type 1 hypersensitivity reaction mediated by an immunoglobulin E (IgE) antibody. The present invention relates to antibodies and antigen binding fragments thereof that bind to one or more of tree pollen allergens Bet v 1, Aln g 1, Cor a 1 and Quue a 1, resulting in inhibition of IgE-mediated anaphylaxis.

Tree pollen allergy is a seasonal allergy caused by various tree pollens suspended in the air. This allergy affects a significant proportion of the population, particularly in Europe and North America, and is characterized by symptoms such as sneezing, nasal congestion, runny nose, itchy or watery eyes, and throat inflammation. These symptoms typically occur in spring when trees such as birch, alder, hazel, and oak release pollen into the air. Tree pollen allergy is a type 1 hypersensitivity reaction mediated by immunoglobulin E (IgE) antibodies. This invention relates to antibodies and their antigen-binding fragments that bind to one or more tree pollen allergens, Bet v 1, Aln g 1, Cor a 1, and Que a 1, and inhibit the IgE-mediated allergic reaction.

The invention belongs to the technical field of medicine, and discloses an artemisia annua pollen allergen sublingual drop as well as a preparation process and application thereof.The preparation is realized through the following core processes: firstly, performing raw material screening and supercritical CO2 degreasing pretreatment on artemisia annua pollen at the full-bloom stage in North China, then performing graded ultralow-temperature freezing and crushing, and performing freeze drying to obtain the artemisia annua pollen allergen sublingual drop. The particle size D90 of the superfine powder is smaller than or equal to 4 microns; then adopting a neutral protease and pectinase composite enzymatic hydrolysis system, and combining 4 DEG C low-temperature pulse ultrasound-assisted treatment to accelerate the dissolution of allergens; carrying out tangential flow grading purification and buffer solution replacement by virtue of 100kDa and 5kDa PES (Polyether Sulfonate) ultrafiltration membranes, and introducing a compound stabilizer; and finally, performing three-stage sterile filtration, and performing encapsulation and preservation. According to the process, through multi-link collaborative optimization, the extraction efficiency and activity stability of allergens are improved, and the prepared sublingual drops have the effects of improving allergic rhinitis, allergic asthma and airway inflammation and also have excellent permeability.

This invention relates to the field of biopharmaceutical technology, and more particularly to a type of beeswax ( Fraxinus pennsylvanica This invention relates to a pollen allergen composition, its preparation method, and its application. It reveals that Chinese ash pollen contains more different allergenic proteins compared to European and American ash pollen. Therefore, using Chinese ash pollen as raw material, the resulting ash pollen allergen composition contains novel allergenic protein components that bind more strongly to the serum of Chinese ash pollen allergy patients, resulting in superior efficacy. This invention utilizes the ash pollen allergen composition to prepare a kit. This kit is highly sensitive, highly specific, and easy to operate. By changing the enzyme conjugate, it can be used for two purposes in one kit, achieving dual functions: detection of ash pollen allergen-specific IgE antibodies (in vitro allergen diagnosis) and quantitative detection of IgG antibodies (evaluation of desensitization therapy efficacy) in allergy patients.

The invention discloses an mRNA pollen allergy vaccine and application, and belongs to the technical field of biological medicine. A coding gene segment of the pollen allergen mRNA disclosed by the invention comprises nucleotides as shown in SEQ ID NO.1-4; the pollen allergen mRNA contains at least one of 5 'UTR, 3' UTR and PolyA, the mRNA pollen allergy vaccine is obtained after the pollen allergen mRNA is loaded by the vaccine vector, and the obtained mRNA pollen allergy vaccine can effectively promote generation of antigen-specific regulatory T cells (Treg) and Th1-sex immune response, so that the treatment of pollen allergy is realized. A new thought is provided for the development of pollen allergy treatment and prevention vaccines.

The invention provides a pollen vaccine based on an aluminum-CpG composite adjuvant and application of the pollen vaccine. The pollen vaccine comprises an aluminum-CpG chemical coupling nano adjuvant and a recombinant mugwort main allergen, the adjuvant is a substance formed by coupling an aluminum oxygen hydride nanorod and CpG through an amido bond, carboxyl on the surface of an AlOOH nanorod is activated, and then the AlOOH nanorod reacts with aminated CpG ODN to obtain the adjuvant. Stable combination of the aluminum salt nanoparticles and CpG ODN is achieved through chemical coupling, the coupling efficiency is larger than or equal to 95%, cytotoxicity is avoided, the problems that in traditional physical mixing, the aluminum salt nanoparticles and CpG ODN are not synergistic in effect and prone to dissociation are solved, and the synergistic immunoregulation effect of adjuvant components is ensured; after the adjuvant is loaded with the Artemisia pollen allergen Art v1, the IgE level can be remarkably reduced, lung inflammation can be relieved, allergic symptoms can be improved in treatment and prevention models, and a novel adjuvant platform is provided for immunotherapy of pollen allergy.

The application provides a preparation method of Artemisia sphaerocephala pollen allergen and an Artemisia sphaerocephala pollen allergen injection, and belongs to the technical field of pollen allergen vaccine preparation. After the Artemisia sphaerocephala pollen is defatted and dried, the Artemisia sphaerocephala pollen is extracted by using an extraction solution, the supernatant is filled into a dialysis bag to be dialyzed, concentrated, enzymolyzed and desalted, and then chromatography is carried out, and polypeptides with molecular weights of 23-46KD and 67-82KD are collected to be the Artemisia sphaerocephala pollen allergen. The Artemisia sphaerocephala pollen allergen injection comprises the Artemisia sphaerocephala pollen allergen, sodium chloride, PBS buffer, phenol and thiourea. The Artemisia sphaerocephala pollen allergen injection of the application fills the blank of Artemisia sphaerocephala pollen allergen injection, can effectively reduce the allergic degree of guinea pigs to Artemisia sphaerocephala pollen, and can be stably stored at 2-8 DEG C.

The invention discloses a method for constructing a Yunnan pine pollen allergy animal model, which belongs to the technical field of Yunnan pine pollen allergy prevention and treatment and comprises the following steps: preparing Yunnan pine pollen allergens and sensitizing non-human mammals. The sensitization treatment comprises a basic sensitization treatment stage and an excitation sensitization treatment stage: in the basic sensitization treatment stage, peritoneal sensitization is carried out once a week and is continued for four weeks; in the nasal-drop sensitization treatment stage, different test substances with the concentration 2 times that of the basic sensitization stage are used for continuous 7-day quantitative nasal-drop excitation, and the nasal drop dosage is 2 microliters / g, and the symptoms of the mouse are observed within 10 minutes after nasal-drop excitation is completed each time. The allergic animal model constructed by adopting the crude extract of the pinus yunnanensis pollen allergen is the first animal model research related to pinus yunnanensis allergy at present, and a reference basis can be provided for subsequent experimental research on the aspect of deep research on pinus yunnanensis pollen allergy; a research model is provided for clinical research on the Yunnan pine pollen allergy or screening of medicines for resisting the Yunnan pine pollen allergy.

The present application relates to the technical field of biology, and particularly relates to a broussonetia papyrifera pollen allergen extract, a preparation method and application thereof.The present application provides a broussonetia papyrifera pollen allergen extract, which comprises a peptide segment composition; and the amino acid sequence of the peptide segment composition is shown as SEQ ID NO:1 to SEQ ID NO:104.The present application provides a commercialized broussonetia papyrifera allergy diagnosis reagent, which can meet the diagnosis and treatment requirements of broussonetia papyrifera pollen allergy patients in the clinic.

The invention belongs to a medical health care product, and particularly relates to an anti-allergic mask for improving a breathing microenvironment and a preparation method of the anti-allergic mask. The outer layer of a traditional N95 mask is replaced with the super-hydrophilic N-TiO2 loaded chitosan fiber membrane (N-TiO2 (at) CS), the membrane can instantly absorb moisture and intercept pollen allergens, the pollen allergens are photocatalytically degraded, and the porous structure of the membrane guarantees low-resistance air permeability; the inner layer is made of polypropylene (PP) melt-blown cloth which is hydrophilically modified by sodium alginate, and exhaled moisture is continuously exhaled to keep dry and comfortable. By means of the design, efficient filtering is maintained, and meanwhile the breathability and hygroscopicity of the mask are remarkably improved.

The application discloses a quality control kit for preparing Humulus pollen allergen extract, which comprises (a) Humulus pollen allergen extract as a control sample, which is used for qualitative or quantitative detection by comparing characteristic protein signals of the sample to be detected; (b) allergen monomers contained in characteristic bands of the extract and combinations thereof, which can be derived from natural purification or recombinant expression purification, and solid-phase or liquid-phase carriers to which the standard samples are attached; (c) antibodies or specific probes or other specific binding substances capable of specifically binding to the allergens in (b), which are used for quantitatively detecting effective allergen levels in the prepared Humulus pollen allergen extract.

The invention discloses an airborne pollen allergen identification and early warning method based on remote sensing data and an early warning system thereof, and relates to the field of smart city forestry management and disease prevention. In order to overcome the defects that an existing pollen monitoring system cannot effectively utilize remote sensing data, prediction precision is low and pollen diffusion is not sufficiently considered, multi-source satellite remote sensing data containing allergen vegetation features are obtained and stored, the multi-source satellite remote sensing data are processed, and a spectral feature library is obtained; carrying out identification and regional clustering on the target vegetation in the spectral feature library; carrying out airborne pollen exposure analysis on the target vegetation subjected to regional clustering to obtain a pollen concentration diffusion risk condition; and carrying out grading and early warning on the pollen concentration diffusion risk condition. The airborne pollen allergen early warning method is mainly used for identifying and early warning airborne pollen allergens in regions with high vegetation density.

The invention discloses a mugwort and ragweed pollen allergen detection kit and a detection method thereof. The kit comprises an incubation tank, a sample diluent, a sample cleaning solution, an enzyme-labeled antibody binding solution, a substrate solution and a test strip, the test strip is provided with a positive control area, a negative control area and a plurality of test areas for pollen allergens of mugwort, ragweed and absinese wormwood; the detection method comprises the following steps: S1, balancing to room temperature; s2, performing incubation; s3, cleaning is conducted; s4, binding an antibody; s5, cleaning; s6, mixing the substrates; and S7, obtaining a color development result. The artemisia argyi and ragweed pollen allergen detection kit has the beneficial effects that the artemisia argyi and ragweed pollen allergen detection kit disclosed by the invention can be used for qualitatively detecting various allergen components of allergic patients, can meet the requirements of the patients and fully reduces the detection cost, and the kit is low in manufacturing cost, can be widely popularized and used and is simple and convenient to prepare; the detection method is simple, easy to operate, time-saving and labor-saving in detection and high in detection efficiency.