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Artemisia pollen allergen vaccine, and preparation method and application thereof

An allergen and pollen technology, applied in the field of allergen vaccines, can solve the problems of Artemisia genus pollen, unknown curative effect of the composition, and no specific disclosure of the composition, etc., to achieve improved effectiveness, good safety and stability , good curative effect

Inactive Publication Date: 2012-07-11
北京新华联协和药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the patent application only mentions that Artemisia pollen allergens can be prepared into a composition. It only has such an idea, and does not specifically disclose how the composition is prepared, the ratio of the allergen, and the prepared composition The curative effect is unknown, that is, the technical problem of whether Artemisia pollen can be used to prepare a combined vaccine has not been solved in essence.

Method used

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  • Artemisia pollen allergen vaccine, and preparation method and application thereof
  • Artemisia pollen allergen vaccine, and preparation method and application thereof
  • Artemisia pollen allergen vaccine, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Artemisia grandis pollen allergen stock solution

[0033]1) Collect the pollen of Artemisia grandis through mechanical (vacuum cleaner) collection or hydroponics, and then use a 300-mesh sieve to remove impurities such as dust, and conduct purity testing, morphological and molecular biology identification on the collected pollen.

[0034] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:5, and degrease under intermittent stirring at room temperature for 3 hours each time.

[0035] 3) Add 1,000 g of degreased Artemisia grandis pollen to 25,000 ml, 200 mM, pH 8.0 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:25 (W:V), and stir at 4°C for 24 hours. Centrifuge the extracted extract at 6000rpm at 4°C for 15min; take the supernatant after centrifugation at 8000rpm at 4°C for 30min.

[0036] 4) Clarify and filter the extract after the extraction with an 8 μm filter membrane, and then filter the c...

Embodiment 2

[0038] Example 2 Preparation of Artemisia annua pollen allergen stock solution

[0039] 1) The pollen of Artemisia annua was collected by mechanical (vacuum cleaner) collection or hydroponic method, and then impurities such as dust were removed using a 300-mesh screen, and the collected pollen was tested for purity, morphological and molecular biological identification.

[0040] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:2, and degrease with intermittent stirring at room temperature for 3 hours each time.

[0041] 3) Add 1,000 g of degreased Artemisia annua pollen to 25,000 ml, 50 mM, pH 8.0 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:10 (W:V), and stir at 3° C. for 12 hours. Centrifuge the extracted extract at 6000rpm at 4°C for 15min; take the supernatant after centrifugation at 8000rpm at 4°C for 30min.

[0042] 4) Clarify and filter the extract after the extraction with an 8 μm filter membrane, and then filte...

Embodiment 3

[0044] Example 3 Preparation of mugwort pollen allergen stock solution

[0045] 1) Collect Artemisia mugwort pollen by mechanical (vacuum cleaner) collection or hydroponics, and then use a 300-mesh sieve to remove dust and other impurities, and conduct purity testing, morphological and molecular biology identification on the collected pollen.

[0046] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:10, stir intermittently at room temperature to degrease, 3 hours each time, until the supernatant acetone is colorless after degreasing, and dry the degreased solid naturally until there is no acetone smell After weighing.

[0047] 3) Add 1000 g of degreased mugwort pollen to 25000 ml, 500 mM, pH 8.0 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:50 (W:V), and stir at 2°C for 24 hours. Centrifuge the extracted extract at 6000rpm at 4°C for 15min; take the supernatant after centrifugation at 8000rpm at 4°C for 30min.

[0048] ...

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Abstract

The invention provides an artemisia pollen allergen vaccine, and a preparation method and application thereof. The artemisia pollen allergen vaccine comprises an artemisia sieversiana pollen allergen, an artemisia annua pollen allergen and an artemisia argyi pollen allergen. Compared with a single artemisia pollen allergen vaccine, the combined vaccine has the advantages that the effectiveness of treatment is improved while the safety can be ensured; and the artemisia pollen allergen vaccine can be used for treating allergic dermatitis, chronic urticaria, allergic rhinitis and allergic asthma which are caused by allergy to multiple artemisia pollens.

Description

technical field [0001] The invention relates to an allergen vaccine, in particular to an Artemisia pollen allergen vaccine, its preparation method and application, and belongs to the field of biotechnology. Background technique [0002] People's understanding of allergen diseases began with "hay fever". In 1911, scientists Noon and Freeman first used pollen extracts to treat hay fever, and the history of allergen disease treatment began from then on. The development of allergens in my country was introduced from the United States in 1956 by Professor Zhang Qingsong, director of the Otorhinolaryngology Department of Union Medical College Hospital, and made a breakthrough. He discovered that the most important autumn allergenic pollen in China is Artemisia plant pollen. In the 1980s, Ye Shitai and Qiao Bingshan led dozens of hospitals across the country to conduct a survey of airborne allergenic pollen in China, which showed that Artemisia pollen was the most pollen in the air...

Claims

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Application Information

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IPC IPC(8): A61K39/35A61P37/08
Inventor 白彩明裴潇竹姜敏汤承祁何立峰牛占坡乔秉善
Owner 北京新华联协和药业有限责任公司
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