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Artemisia pollen allergen vaccine and preparation method thereof

An allergen and pollen technology, applied in the directions of allergen antigen components, respiratory diseases, skin diseases, etc.

Active Publication Date: 2012-06-27
北京新华联协和药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there is still no clinical evidence linking aluminum-containing vaccines to the development of neurodegenerative disorders such as Alzheimer's disease in humans, a link has been postulated and widely debated

Method used

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  • Artemisia pollen allergen vaccine and preparation method thereof
  • Artemisia pollen allergen vaccine and preparation method thereof
  • Artemisia pollen allergen vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Example 1 Preparation of Artemisia grandis pollen allergen stock solution

[0030] 1) The pollen of Artemisia grandis was collected mechanically (vacuum cleaner), and then impurities such as dust were removed using a 300-mesh sieve, and the collected pollen was tested for purity, morphological and molecular biological identification.

[0031] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:5, and degrease under intermittent stirring at room temperature for 3 hours each time.

[0032] 3) Add 1000 g of degreased Artemisia grandis pollen to 25000 ml of 200 mM pH8.0 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:25 (W:V), and stir at 4°C for 24 hours.

[0033] 4) Centrifuge the extracted extract at 6000rpm at 4°C for 15min. Take the supernatant after centrifugation at 8000rpm at 4°C f...

Embodiment 2

[0037] Example 2 Preparation of Artemisia annua pollen allergen stock solution

[0038] 1) The pollen of Artemisia annua was collected mechanically (vacuum cleaner), and then impurities such as dust were removed using a 300-mesh sieve, and the collected pollen was tested for purity, morphological and molecular biological identification.

[0039] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:5, and degrease under intermittent stirring at room temperature for 3 hours each time.

[0040] 3) Add 1,000 g of defatted Artemisia annua pollen to 25,000 ml of 200 mM pH 8.0 disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:25 (W:V), and stir at 4° C. for 24 hours.

[0041] 4) Centrifuge the extracted extract at 6000rpm at 4°C for 15min. Take the supernatant after centrifugation at 8000rpm at 4°C for 30min.

[0042] 5) Clarify and filter the extract after extraction with an 8 μm filter membrane, and then filter the crude extract thr...

Embodiment 3

[0045] Example 3: Preparation of mugwort pollen allergen stock solution

[0046] 1) Collect mugwort pollen mechanically (vacuum cleaner), then use a 300-mesh sieve to remove dust and other impurities, and conduct purity testing, morphological and molecular biology identification on the collected pollen.

[0047] 2) Add the pollen obtained in 1) to acetone at a volume ratio of 1:5, stir intermittently at room temperature for degreasing, each time for 3 hours, until the supernatant acetone is colorless after degreasing, and dry the degreasing solid naturally until there is no smell of acetone After weighing.

[0048] 3) Add 1000 g of degreased mugwort pollen to 25000 ml of 200 mM disodium hydrogen phosphate-sodium dihydrogen phosphate buffer at a feed ratio of 1:25 (W:V), and stir at 4° C. for 24 hours.

[0049] 4) Centrifuge the extracted extract at 6000 rpm at 4° C. for 15 min. Take the supernatant at 8000rpm, centrifuge at 4°C for 30min and take the supernatant.

[0050] 5...

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Abstract

The invention provides an artemisia pollen allergen injection vaccine comprising artemisia pollen allergen and an L-tyrosine adjuvant, wherein the artemisia pollen allergen comprises sievers wormwood herb pollen allergen, sweet wormwood herb pollen allergen, and mugwort pollen allergen. The activity of the sievers wormwood herb pollen allergen is 1-1000AU / ml, the activity of the sweet wormwood herb pollen allergen is 1-1000AU / ml, and the activity of the mugwort pollen allergen is 1-1000AU / ml. The invention also provides a preparation method of the vaccine, and an application of the vaccine in preparing medicines used for treating anaphylactic diseases. According to the invention, three types of artemisia pollen allergen are adopted, such that the efficacy is higher than that of single artemisia pollen allergen. The artemisia pollen allergen is adsorbed onto the L-tyrosine adjuvant, such that an in-vivo release-retarding effect is provided, and efficacy and safety of the medicines are improved. The administration dosage of the vaccine during an entire treatment period is greatly lower (by approximately 100 times) than that of sublingual drops. With the vaccine, the treatment cost is greatly lower than that of sublingual drops immunological desensitization, such that patient burden of medical treatment is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a vaccine for preventing and treating Artemisia pollen-induced allergic rhinitis, asthma and allergic dermatitis specific immunotherapy and a preparation method thereof. Background technique [0002] People's understanding of allergic diseases began with "hay fever". In 1911, scientists Noon and Freeman used pollen extract to treat hay fever for the first time, and the history of allergen disease treatment began from then on. The development of allergens in my country was introduced from the United States by Professor Zhang Qingsong, director of the Department of Otorhinolaryngology of Union Medical College Hospital in 1956, and made a breakthrough. He discovered that the most important autumn-type allergenic pollen in China is Artemisia plant pollen. China's own allergens provide a basis for realizing the localization of allergens. In the 1980s, Ye Shitai and Qiao Bingsh...

Claims

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Application Information

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IPC IPC(8): A61K39/35A61P11/02A61P11/06A61P17/00A61P37/08
Inventor 白彩明裴潇竹姜敏汤承祁何立峰牛占坡乔秉善
Owner 北京新华联协和药业有限责任公司
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