The invention relates to a prescription with functions of reducing toxicity, enhancing efficacy and inhibiting tumors, and application of the prescription. The prescription comprises radix pseudostellariae, leech, semen arecae, agarwood, rhizoma belamcandae, radix bupleuri, flos chrysanthemi indici, herba scutellariae barbatae, Japanese climbing fern spore, earthworm, rhizoma amorphophalli, radixpulsatillae chinensis, herba lysimachiae, squama manitis, galla chinensis, fructus rubi, rhizoma polygonati odorati, rhizoma paridis, scorpion, calculus bovis factitius, cornu saigae tatariace powder,fructus amomi, raw pollen typhae, herba taraxaci, poria cocos, cortex moutan, radix rehmanniae and turtle shell. The prescription can strengthen the healthy qi of a human body, inhibit the angiogenesis around cancers, dredge the passage so as to enable cancer toxins to be discharged out of the human body, and promote the cancer toxins to induce yin heat into qi phase so as to enable the cancer toxins to be smoothly discharged by relieving bowels.