Medicinal composition for inhibiting angiogenesis and application thereof

A pharmaceutical composition and angiogenesis technology, which are applied in the directions of drug combinations, medical preparations containing active ingredients, pharmaceutical formulations, etc., to achieve a good effect of inhibiting angiogenesis.

Active Publication Date: 2010-01-20
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] It can be seen that there are still many deficiencies in the above-mentioned c

Method used

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  • Medicinal composition for inhibiting angiogenesis and application thereof
  • Medicinal composition for inhibiting angiogenesis and application thereof
  • Medicinal composition for inhibiting angiogenesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The present invention is illustrated by the following examples, but the present invention is not limited by the following examples. Example 1 The cultivation of mycelia with the mycelium of Shanzhi Zhi (BCRC 36937)

[0039] The present invention is based on the patent certificate number I287991 of the Republic of China, the preparation method of the active substance of Cedarwood mycelia and the Cedarwood mycelium contained in the composition and the cultivation method, so as to obtain enough Cedarwood fermented liquid for freeze-drying Powder, briefly describe the cultivation method.

[0040] Mycelium strains:

[0041] It is the strain BCRC 36937 deposited in the Institute of Food Industry.

[0042] Plate culture:

[0043] The mycelium was inoculated on a plate, and cultured at 25° C. for about 2 weeks using potato dextrin medium (Potato Dextrose Agar, PDA).

[0044] Flask culture:

[0045] Scrape the hyphae on the plate and inoculate into the flask, use the ...

Embodiment 2

[0059] Example 2 Separation of the active components of the lyophilized powder of the fermented broth of Shanzhizhi for inhibiting angiogenesis

[0060] Carry out the freeze-drying of the liquid culture suspension of the mycelia of Cedarwood obtained in Example 1, including the mycelium and the clarified liquid, to obtain the lyophilized powder of the fermented liquid of C. figure 1In the purification steps shown, take 1 kg of Chinese fir fermentation broth freeze-dried powder, extract three times with four liters of methanol (MeOH) to obtain methanol extract, and concentrate to obtain methanol extract. The methanol extract was preliminarily separated on a silica gel chromatography column (60-230mesh, 5×60cm), followed by n-hexane (n-hexane) / ethyl acetate (EtOAc) at a ratio of 10:1 to 0:1 Punch and lift, get 7 divisions. It belongs to fraction 4 obtained by eluting with n-hexane / ethyl acetate (1:1 to 1:4), using Sephadex LH-20 chromatography column (3×60cm), and eluting with...

Embodiment 3

[0061] Example 3 Identification of the Active Components of the Freeze-dried Powder of the Fermented Broth of Shanzhi Zhi

[0062] The chemical structures of the three compounds obtained in Example 2 were identified, and the chemical structures of the compounds were analyzed with a mass spectrometer and a high-resolution gas chromatography-mass spectrometer.

[0063] 1. Identification of the chemical structure of compound As-4

[0064] Dark yellow solid, MS (mass spectrometer, Finnigan GCQ) measured molecular weight of 152, by 1H-NMR and 13C-NMR (d-solvent: CDCl 3 ) (NMR instrument, Varian Unity Inova-500Spectrometer, 500MHz) measurement comparison, δH 2.07 (3H, d, J=1.5Hz) is the methyl signal at the C-7 (δC15.7) position, δH 3.82 ( 3H, s) is the methoxy signal at C-2 (δC 56.5), δH 5.88 (1H, d, J=2.5Hz) and δH 6.53 (1H, d, J=1.5Hz) are at C-3 (δC107.5) and C-5 (δC 134.1) position of the aromatic hydrogen signal, and then by HRMS (high resolution gas chromatography mass spec...

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Abstract

The invention relates to a medicinal composition for inhibiting angiogenesis and application thereof. The medicinal composition comprises any one composition of 2-methoxy-6-methyl-P-benzoquinone(2-methoxy-6-methyl-p-benzoquinone), 2,3-dimethoxy-5-methyl-P-benzoquinone(2,3-dimethoxy-5-methyl-p-benzoquinone) or 2-hydroxy-5-methoxy-3-methyl-P-benzoquinone(2-hydroxy-5-methoxy-3-methyl-p-benzoquinone) and appropriate pharmaceutically acceptable excipients or supporting agents; and the medicinal composition is used for inhibiting the angiogenesis and further treats or prevents angiogenesis-related diseases.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for inhibiting angiogenesis and its application, in particular to a pharmaceutical composition for inhibiting angiogenesis-related diseases and its application. Background technique [0002] Angiogenesis is a normal physiological change and is common in the process of development and wound healing. In recent years, it has been found that angiogenesis is closely related to the formation of tumors. When cancer cells form a tumor, the cancer cells themselves or the surrounding connective tissue secrete angiogenesis factors, which will promote the following changes in endothelial cells: (1) decomposition and destruction of connective tissue around the tumor; (2) ) endothelial cell hyperplasia; (3) endothelial cells move towards the position where angiogenesis factors are secreted; (4) endothelial cells recombine to generate new blood vessels. Angiogenesis is very important for tumor formation. ...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00A61P43/00
Inventor 陈劲出许胜杰林定威陈炎鍊林宜瑾
Owner GRAPE KING
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