Compounds from antrodia camphorata, method for preparing the same and use thereof

一种提取物、药物的技术，应用在牛樟芝提取物及其制备领域，能够解决抗癌效果所知甚少等问题，达到抑制多种癌细胞增生、抑制效果提升、抑制血管新生的效果

Active Publication Date: 2016-07-06
ONENESS BIOTECH
View PDF13 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In summary, Antrodia Cinnamomea contains substances with anti-cancer potential and has been proven to be effective against a variety of cancers, but little is known about whether there are novel compounds and which compounds have anti-cancer effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compounds from antrodia camphorata, method for preparing the same and use thereof
  • Compounds from antrodia camphorata, method for preparing the same and use thereof
  • Compounds from antrodia camphorata, method for preparing the same and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation of embodiment 1 Antrodia camphorata extract

[0058] The liquid fermented mycelium of Antrodia camphorata is refluxed twice with n-hexane, each time for 1-3 hours; after suction and filtration, the two n-hexane extracts are combined; the silica gel with 70-230 holes is taken (silicagel) (70-230mesh) and mycelium weight 1:1 (w / w) to fill the column, elute with n-Hexane / Ethylacetate (Ethylacetate) gradient, and the eluate is divided into fractions (fraction) F1 , F2 and F3, the gradients are 17-22%% Ethylacetate, 23-27% Ethylacetate and 28-33% Ethylacetate respectively, and F3 is divided into three sections F3-1-F3-3 according to the order of retention time.

[0059] Get F3-1 and use mobile phase as dichloromethane / acetone (CH 2 Cl 2 / Acetone) is separated by silica gel column chromatography (gradient elution from 50:1 to 20:1 in polarity), and the section of 40:1-15:1 is taken, and the normal phase HPLC (Medium pressure liquid chromatography) semi-prepa...

Embodiment 2

[0066] The chemical structure analysis of embodiment 2 Antrodia camphorata extract

[0067] For the aforementioned purified Antrodia camphorata extract, its chemical structure was identified by spectroscopic analysis, including the use of 1D / 2 DNMR and mass spectrometry. The structural analysis results of the compound are as follows:

Embodiment 21

[0069]

[0070] EIMS, m / z 471.2701[M+Na]+; 1 HNMR (400MHz, CD 3 OD)δ5.77(1H,d,J=3.2Hz,H-4),5.18(1H,t,J=5.6Hz,H-12),5.16(1H,t,J=6.4Hz,H-8 ),5.11(1H,dt,J=1.6,8.8Hz,H-16),4.43(1H,q,J=6.8,H-15),4.00(3H,s,H-24),3.62(3H, s,H-23),2.53(1H,m,H-6),2.29(1H,m,H-7a),2.24(2H,m,H-14),2.11(2H,m,H-11) ,2.10(3H,s,-OAc),2.08(1H,m,H-10a),2.02(1H,m,H-7b),1.94(1H,m,H-10b),1.92(1H,m, H-5), 1.71(3H,d,J=1.2Hz,H-18),1.66(3H,d,J=1.2Hz,H-19),1.64(3H,s,H-20),1.58( 3H, s, H-21), 1.18 (3H, d, J=6.8Hz, H-22); 13 CNMR (100MHz, CD 3 OD)δ199.2(s,C-1),171.6(s,-COCH 3 ),160.7(s,C-3),138.9(s,C-2),138.8(s,C-9),134.9(s,C-17),132.9(s,C-13),129.6(d ,C-16),128.4(d,C-12),122.2(d,C-8),70.4(d,C-4),68.1(d,C-15),61.2(q,C-23) ,60.4(q,C-24),49.2(t,C-14),44.4(d,C-5),42.6(d,C-6),40.9(t,C-10),28.1(t, C-7), 27.6(t, C-11), 26.1(q, C-18), 21.0(q, -COCH 3 ), 18.5 (q, C-19), 16.8 (q, C-20), 16.5 (q, C-21), 13.3 (q, C-22).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to compounds from antrodia camphorata, a method for preparing the same and use thereof. The compounds has the structural characteristics of formula I-III, capable of effectively suppressing new growth of blood vessels and proliferation of cancer cells including liver cancer, brain cancer, prostate cancer, breast cancer, colorectal cancer, or melanoma. The effect of suppressing proliferation of cancer cells for liver cancer and colorectal cancer is better.

Description

technical field [0001] The invention relates to a fungal extract, in particular to an antrodia camphorata extract, a preparation method and application thereof. Background technique [0002] Antrodia camphorata (formerly named Antrodiacinnamomea) is a fungus parasitic in the hollow trunk of the camphor tree (Cinnamomum kanchirai Hayata, Lauraceae), and it is a unique medicinal fungus in Taiwan. In Taiwanese folk medicine, Antrodia camphorata is a good antidote, which can be used for food poisoning or pesticide poisoning. It is also commonly used in folk medicine to treat hepatitis or liver-related diseases. Because it has high medical value, but it is not easy to cultivate and grows slowly, so there are already studies on the genetics and metabolomics of Antrodia Cintrodia (Luetal. Learn more. [0003] Antrodia Antrodia contains a variety of biologically active substances that can be used for medicinal purposes, including polysaccharides with large molecules and terpenes w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/21C07C67/48C07C67/56C07C49/753C07C45/78C07C45/79C07C49/577C07D307/33A61K31/22A61K31/122A61P35/00
CPCC07C45/64C07C45/78C07C45/79C07C49/577C07C49/753C07C67/48C07C67/56C07C69/21C07D307/33C07C2601/16A61K31/22A61P35/00A61K36/07A61K2236/00A61K2300/00
Inventor 刘琼琳蔡潍泽谢凯欣
Owner ONENESS BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap