89results about How to "Maintain cell viability" patented technology

PCT No. PCT/US97/09300 Sec. 371 Date Feb. 10, 1999 Sec. 102(e) Date Feb. 10, 1999 PCT Filed Jun. 10, 1997 PCT Pub. No. WO98/56893 PCT Pub. Date Dec. 17, 1998The object of the invention is to provide a method and apparatus for treating membrane containing material with electrical fields and with an added treating substance. With the method, a plurality of electrodes (121-128) are arrayed around the material to be treated and are connected to outputs of an electrode selection apparatus (110). Inputs of the electrode selection apparatus are connected to outputs of an agile pulse sequence generator. A treating substance is added to the membrane-containing material. Electrical pulses are applied to the electrode selection apparatus and are routed through the electrode selection apparatus in a predetermined, computer-controlled sequence to selected electrodes in the array of electrodes, whereby the membrane containing material is treated with the added treating substance and with electrical fields of sequentially varying directions. The routing of applied pulses through the electrode selection apparatus (110) to selected electrodes (121-128) can be done in an enormous number of ways.

A package for a medical device, the package capable of sustaining viable cells, wherein the package includes a first container, which is configured to receive a medical device and to sustain at least one viable cell, and a fluid port in communication with the first container for allowing sterile passage of an agent to the medical device and for maintaining cell viability.

Improved coring devices suitable for articular cartilage and bone, wherein the cutting device is capable of slicing through a tough protective tangential zone, delicately separating the shock absorbing columns of cells in the radial zone of the cartilage, and finally cutting into the hard underlying bone in a manner that preserves the viability of osteochondral cells. The coring device features an annulus having a flat annular cutting edge interrupted by at least one serration having neutral cutting angles. A method for concurrently removing cartilaginous and bony tissue using an improved coring device that preserves the viability of osteochondral cells.

The invention relates to a method for expanding activated lymphocyte, comprising the steps of allowing biologic sample containing lymphocytes to contact immobilized anti-CD 3 antibodies, then cultivating activated lymphocytes with culture mediums in different IL-2 concentrations to expand the activated lymphocytes. The invention also relates to a cultivation system used for expanding activated lymphocytes. The expanded activated lymphocytes can be applied in the immunotherapy of tumor, infectious diseases, and congenital or acquired immunodeficiency diseases.

The invention puts forward a universal cancer organoid in-vitro culture medium, which consists of a DMEM/F-12K (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12K) basic culture medium and an adding factor, wherein the adding factor consists of HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid), L-Glutamine, EGF (epidermal growth factor), Noggin, FGF (fibroblast growth factor)-10, A83-01 and Y27632. According to the culture medium of the embodiment of the invention, the success rate of organoid passage culture can be greatly improved, in addition, the universal culture of cancer samples, including gastric carcinoma, rectal carcinoma, lung carcinoma and the like, can be realized, culture cost is greatly lowered, the long-term culture of the organoid can be realized, and abiobank is established.

The invention relates to a positive circulating tumor cell enrichment method. The positive circulating tumor cell enrichment method comprises the following steps that S1, a buffer solution and a tumorpatient blood sample are sequentially added in a cell separation solution, and first-time centrifugal treatment is performed; S2, the tumor patient blood sample obtained after the first-time centrifugal treatment is subjected to second-time centrifugal treatment, supernate is removed, red blood cell lysate is added, and then low-temperature splitting decomposition is performed; S3, the tumor patient blood sample obtained in the step S2 is subjected to third-time centrifugal treatment, supernate is removed, incubation liquid is added, then immunomagnetic beads are added, incubation is performed after even mixing, and the immunomagnetic beads are coupled with epithelial cell adhesion molecules; S4, the incubation liquid is removed after incubation, and elution is performed to obtain a target; S5, the target obtained in the step S4 is transferred to a complete culture medium, and continuous culture is performed to obtain circulating tumor cells. The positive circulating tumor cell enrichment method has the advantages of being accurate in capture, capable of keeping cell viability to the most degree, low in cost and efficient.

The invention discloses a method for cell surface functional coating modifying. The method comprises the following steps: putting cells into a dopamine solution prepared by a Tris-hydrochloric acid buffer solution; performing oscillating reaction for 20 minutes to 8 hours; enabling dopamine to generate oxidative polymerization on a cell membrane surface; enabling a dopamine quinone compound and dopamine to generate disproportionated cross-linking reaction; forming a composite layer on the cell surface; and modifying the cell surface functional coating for enabling the cell to keep cell activity in an unfriendly environment. The method is gentle in condition and is simple to operate; and moreover, the whole process is completed in an aqueous solution, so that possibility of generating pollution on environment by the solvent is avoided; and functional coating modification, on the whole cell surface, by dopamine is achieved through one step.

The invention discloses an artificial floating bed system for purifying water quality by using superfine fibers. The artificial floating bed system comprises a floating bed, a planting basket, planting basket filler, floating bed plants, superfine fiber three-dimensional artificial waterweeds, a supporting frame, an aeration device, a microbial preparation and a box body. The invention also discloses a method for purifying water by using the system. The method comprises the following steps of: aerating and oxygenating sewage through micropores, and intercepting, adsorbing and decomposing pollutants in the sewage by a biological membrane attached to the superfine fiber three-dimensional artificial waterweeds in the superfine fiber artificial waterweed restoration area. Pollutants in the water body are absorbed, metabolized and decomposed by microorganisms at the roots of the plants and on the attached filler when flowing through the plant synergistic microorganism remediation area. A microbial agent is added, and microorganisms are used for effectively adsorbing, converting and degrading nitrogen and phosphorus in the water body, so that algae propagation is inhibited, and the waterbody is effectively purified. The artificial floating bed system has the advantages of readily available materials, low construction and operation costs, convenient manufacture, landscape beautification, water quality improvement, and good application prospect.

The present disclosure provides highly multiplexed base editing methods and compositions that minimize the induction of DNA damage sensors in eukaryotic cells and maintain cell viability. The disclosed base editing methods improve the survival of eukaryotic cells after large-scale genome editing. These methods are based upon the discovery that use of a dead Cas9 base editor and optimal cell conditions during and after base editing enhances cells' tolerance to and survival following thousands of edits to the genome. Optimal cell conditions after base editing include the use of a combination of small molecule factors and/or inhibitors. These methods are facilitated by the design and use of tens to hundreds to thousands of gRNAs for guiding the base editor to the target sequences. The disclosed methods are capable of inducing between ten and 300,000 edits to the genome of a eukaryotic cell. Further disclosed are pharmaceutical compositions and compositions of eukaryotic cells comprising fusion proteins and a plurality of unique gRNAs, and a combination of small molecule factors and inhibitors. Also disclosed are kits for the generation of the fusion protein-gRNA complexes described herein.

The invention relates to the field of food processing and particularly provides peanut protein quick-frozen totally-vegetable meat for supplying vitamins and a preparation method thereof. The totally-vegetable meat, which can supply the required minerals to human body, is prepared through quick-freezing technology with peanut drawing protein as a main material with addition of the food materials, such as lentinula edodes, cabbage, algae, carrot, walnuts, brown rice and the like, which are rich in vitamin, and vitamin B which is difficult to intake from vegetable foods, and also with addition of natural preservatives and anti-oxidants, such as nisin, chitosan, red chili pepper extract and the like. The totally-vegetable meat is prepared with plant vegetable replacing meat, improves dietary structure and promotes body health. The peanut drawing protein has a meat-like taste and also the fragrance of peanuts. The totally-vegetable meat can supply the required various vitamins to human body. The pure natural preservatives and anti-oxidants ensure the totally-vegetable meat to be safe. The totally-vegetable meat has the taste which is similar as fresh product and has long shelf life due to the quick-freezing technology.

The invention provides a long-term-preserved immune cell gel preparation and a preparation method thereof. The gel preparation contains immune cells and a gel matrix, wherein the gel matrix mainly contains the following components: deionized water, dextran, a mistletoe leaf extract, propylene glycol alginate, oleyl alcohol and guaiacum. The method comprises the following steps: preparing the mistletoe leaf extract; and dissolving the mistletoe leaf extract into deionized water, standing for one night, adding propylene glycol alginate, oleyl alcohol and guaiacum, stirring, adding dextran, stirring, preparing the gel matrix, adding the immune cells into the gel matrix, and uniformly stirring. According to the preparation method, the cell activity of the immune cells in a long-term preservation process can be guaranteed, meanwhile, the stability of the immune cell gel preparation can be improved, the pesticide effect of the long-term-preserved immune cell gel preparation can be guaranteed, the drug release efficiency can be improved, and the mildewing can be prevented; and the immune cell gel preparation is strong in practicability.

The invention relates to an immune cell freezing medium.The immune cell freezing medium comprises dimethyl sulfoxide, human serum and an AIM-V culture medium.According to the immune cell freezing medium, the recovery survival rate of immune cells can be increased, the cell activity is kept, the survival time of the immune cells is prolonged, and losses of surface antigens of the immune cells are reduced.

The invention discloses a method for preparing panaxoside and panaxynol through enzymatic zymotechnics. The method comprises the following steps: cleaning Changbai mountain ginseng, drying the ginseng at 50 to 60 DEG C, and crushing the ginseng to be 40 meshes; clustering raw material powder in hands, releasing namely dispersing the raw material power, adding mineral water to the raw material power, soaking the raw material power for 18 hours, and adjusting the PH value to be 4.5 to 6.5; putting mixed liquor into an enzymatic zymotechnics tank, adding enzymatic zymotechnics liquid to perform enzymatic zymotechnics, setting the enzymatic zymotechnics temperature to be 35 DEG C and the enzymatic zymotechnics time to be 72 hours, stirring and oxygenating once every 4 hours, and controlling the PH value to be 3.5 to 6.2; performing frame filtering, performing spray drying on supernate, ensuring that the drying temperature does not exceed 65 DEG C, and controlling the moisture to be lower than 7 percent to obtain powder; crushing the powder to be 120-mesh powder, then performing Co60 irradiation sterilization, and packing and warehousing finished products. The method is convenient in technology, easy to operate, low in energy consumption, clean and environment-friendly.

The invention discloses a marine seawater instant freezer used for instantly freezing marine products on a ship. The marine seawater instant freezer is arranged in a cabin or a refrigeratory of a cabin and provided with a freezing tank, a water tank, a refrigeration unit matched with the freezing tank, a conveying system and a hanging basket used for containing marine products. A liquid freezing medium used for freezing marine products is contained in the freezing tank. The hanging basket containing marine products is arranged in the freezing tank, the marine products is placed into the water tank after being cooled through the freezing medium, the surfaces of the marine products are instantly frozen, and therefore instant freezing of marine products is completed. Energy consumption can be greatly reduced, the purpose of instantly freezing marine products on sea better more rapidly is achieved, and the quality and delicious taste of the marine products can be kept.

The invention provides a method for modifying heterogeneous surfaces of gold nanoparticles through hydrophobic ionic liquid, and aims at quickly and efficiently replacing CTAB (Cetyltrimethyl Ammonium Bromide) molecules on the surfaces of the gold nanoparticles with MUA (11-Mercaptoundecanoic Acid) by twice phase transfer. The method is characterized in that the ionic liquid [BMIM] Tf2N is used as a good solvent for extracting the gold nanoparticles from a water phase as well as weakening the dense arrangement of the CTAB on the surface of nanogold, and thus MUA can be well combined with the gold surface; in particular, the gold nanoparticles modified by MUA are water-soluble due to the carboxyl on the surfaces, so that the water phase can be quickly returned by transferring, and as a result, hydrosol of the surface-modified nanogold can be obtained. The method can solve the aforementioned reported shortages of incomplete surface decoration, complex operation and time consumption. With the adoption of the method, the surface modification can be finished within minutes; nearly all CTAB can be replaced with MUA, so that the biotoxicity can be obviously reduced, and moreover, the convenience for further biomolecule modification is ensured, and the application in the biomedicine can be improved.

The invention discloses a solid lactobacillus beverage and a preparation method thereof. The solid lactobacillus beverage contains the following components in parts by weight: 20-40 parts of fermented milk, 5-10 parts of sugar, 0.1-0.5 part of a stabilizer, 0.01-0.05 part of a chelating agent, 0.1-0.5 part of an acidulant, and 0.05-0.1 part of essence. The solid lactobacillus beverage contains active lactobacillus cells and has the efficacy of promoting digestion, maintaining the ecological balance of intestinal microbes and enhancing the immunity of human bodies; meanwhile, the solid lactobacillus beverage is convenient to carry and drink, long in shelf life and suitable for all people; the preparation method is simple and feasible and has important application and popularization values.

The invention provides a method for processing a cell sample in a single cell sorting process. The method comprises the following steps in sequence: 1, acquiring a required cell sample; 2, spreading the cell sample and a water retention substance for keeping cell activity on the surface of a sorting chip; 3, putting the chip on a sorting platform for sorting target cells; 4, putting the sorted cells into a receiving substance in a receiving device; and 5, performing subsequent detection or culture operation on the sorted cells. According to the method, the substance with a water retention function is used, so that the cells can keep the original biological state and biological activity in the sorting process, and detection and enlarged culture of the activity of the cells after sorting arenot influenced.

The invention discloses a hybridoma cell serum-free medium, which comprises the following raw materials: alanine, arginine, aspartic acid, asparagine, cystine, cysteine, glutamine, glutamic acid Amino acid, glycine, histidine, etc., calcium chloride, magnesium sulfate, potassium chloride, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium bicarbonate, sodium hydroxide, ferrous sulfate heptahydrate, magnesium chloride hexahydrate, sulfuric acid Copper, sodium pyruvate, manganese dichloride, sodium selenite, ammonium molybdate, etc., nicotinamide, p-aminobenzoic acid, folic acid, ribose, uracil, lipoic acid, linoleic acid L, cholesterol, dexamethasone, Estradiol progesterone, testosterone, etc. The medium of the present invention does not contain serum components at all; the purity of the produced antibody is greatly improved; the medium has high amplification speed and high antibody expression ability; the medium can maintain cell activity for a long time, and the hybridoma cell culture time is greatly improved Extending can greatly extend the production time and increase the antibody production.

The invention relates to the field of food processing, in particular to nutrient-balanced agaric all-vegetable quick-frozen vegetarian meat and a making method thereof. The making method includes: using agaric as a main material supplemented by soybean tissue protein, wheat gluten and starch; adding food materials like walnut, almond, bamboo shoot and vegetable juice which are rich in nutrient; adding pure natural preservatives like nisin, pectin decomposition product and paprika extract; adopting quick-freezing technology to make the quick-frozen all-vegetable vegetarian meat comparatively balanced in various nutrients. The nutrient-balanced agaric all-vegetable quick-frozen vegetarian meat and the making method have the advantages that various food materials are matched, so that the quick-frozen vegetarian meat is balanced in nutrient; black fungus has efficacy of tonifying qi and strengthening body, nourishing kidney and stomach, activating blood, lowering blood lipid and softening blood vessels, has good dissolving effect on gallstone and kidney stone and is high in pharmaceutical value; the pure natural preservatives and antioxidant are added, so that the quick-frozen vegetarian meat is more assured to eat; the quick-freezing technology is adopted, so that taste as good as that of fresh products can be maintained and quality guarantee time is long.

The survival of cells is limited by introducing into the cells a regulatably expressible gene whose expression results in the formation of a cytoplasmatically active hydrolytic enzyme. Populations of cells containing, in addition to such a regulatably expressible gene, a DNA coding for an immunologically active, pesticidally active or environmental pollutant-degrading gene product, may be useful in immunologically active compositions, pesticidally active compositions and environmental pollutant-degrading compositions, respectively.

The invention discloses hemorheology analytical equipment used for a clinical lab. The hemorheology analytical equipment comprises a main body of the analytical equipment, a PLC controller is arrangedat one side of a front side surface of the main body, a wireless communication module is arranged at one corner of the upper surface of the main body of the analytical equipment, a placing chamber isarranged at the other side of the front side surface of the main body of the analytical equipment, a case door is hinged to one side of an opening end of the placing chamber through a hinge, a handleis arranged at an end part of the side surface of the case door, two guide rails are arranged at the bottom of the placing chamber, a placing case is clamped at the upper end of two guide rails in asliding mode, a linear guide rail is arranged at the middle of the top of the placing chamber, a linear motor is clamped at the lower surface of the linear guide rail in the sliding mode, an electrical telescoping rod is arranged at the lower surface of the linear motor, and a connecting seat is arranged at the telescoping end of the electrical telescoping rod. The hemorheology analytical equipment is convenient for timely cleaning, avoids cross contamination and pipeline obstruction, enables continuous detection, has good sealing performance during a detection process, and avoids the blood pollution by extraneous air.

The invention relates to the technical field of biological preservation, in particular to a biological dormancy preservation system and method. The biological dormancy preservation system comprises acleaning unit, an air-drying disinfection and sterilization unit and a refrigeration dormancy tunnel unit which are sequentially arranged. The refrigeration dormancy tunnel unit comprises a refrigeration tunnel chamber, a temperature detection module, a field control system and a chain transfer module; the initial body temperature of an organism before dormancy is acquired by the aid of the temperature detection module, the field control system receives initial body temperature information of the organism, the initial body temperature of the organism and the dormancy temperature of the organism have a corresponding relationship, the field control system can form a precise control strategy according to the temperature information needed by dormancy of the organism, control chain transfer speed and accordingly control the passing time of the organism in the refrigeration tunnel chamber, so that the organism reaches the dormancy temperature and then is stored and transferred, organism preservation time is long, loss is low, the quality of the preserved organism is not reduced, and preservation cost is low.

The invention mainly relates to the field of planting technology, and discloses a method for promoting the differentiation of Trichosanthes mellifera seedlings, including: selection and treatment of explants, preparation of medium, inoculation of explants, induction of differentiation and cultivation; Induced differentiation during basket tissue culture, after 16 days of culture in B5 medium, yellow-green cell protrusions began to appear, and then differentiated bud clusters appeared, which significantly shortened the time for induction of differentiation, improved planting efficiency, and increased economic income by 16.2%; As an explant, it will not cause damage to the Trichosanthes plant, does not affect the growth and results of Trichosanthes, and the source of young leaves is extensive, and tissue culture can be carried out at any time, and the planting efficiency is significantly improved; Salt water, acetic acid solution and nutrient solution treatment, avoid using too much disinfectant for repeated disinfection, save costs, and allow explants to continue absorbing and accumulating nutrients in a slightly acidic environment, speeding up the process of inducing differentiation of explants.