The invention relates to a positive circulating tumor cell enrichment method. The positive circulating tumor cell enrichment method comprises the following steps that S1, a buffer solution and a tumorpatient blood sample are sequentially added in a cell separation solution, and first-time centrifugal treatment is performed; S2, the tumor patient blood sample obtained after the first-time centrifugal treatment is subjected to second-time centrifugal treatment, supernate is removed, red blood cell lysate is added, and then low-temperature splitting decomposition is performed; S3, the tumor patient blood sample obtained in the step S2 is subjected to third-time centrifugal treatment, supernate is removed, incubation liquid is added, then immunomagnetic beads are added, incubation is performed after even mixing, and the immunomagnetic beads are coupled with epithelial cell adhesion molecules; S4, the incubation liquid is removed after incubation, and elution is performed to obtain a target; S5, the target obtained in the step S4 is transferred to a complete culture medium, and continuous culture is performed to obtain circulating tumor cells. The positive circulating tumor cell enrichment method has the advantages of being accurate in capture, capable of keeping cell viability to the most degree, low in cost and efficient.