69results about How to "Achieve accurate quantification" patented technology

The invention discloses uPAR (urokinase-type plasminogen activator receptor) targeted peptides with the amino acid sequence indicated in SEQ ID NO. 1. The uPAR targeted peptides is linear peptides formed by 13 amino acids, the peptides are easy to synthesize and can form a stable conformation and be combined with uPARs with specific target. The invention discloses a uPAR targeted probe comprisinga single unit and the above uPAR targeted peptides. The uPAR targeted probe is subjected to specific identification of the peptides and is combined with the uPARs, signals for detection of imaging equipment are transmitted through the signal unit, living visual detection of the uPARs is achieved, and distribution and number of the uPARs are displayed visually and are used for disease detection foruPAR expression abnormalities. The invention further discloses a living molecular imaging method. The uPAR targeted probe is utilized, and the living molecular imaging method has the advantages of being safe, non-invasive, dynamic, visual and the like and is suitable for direct detection of human body.

The present invention relates to a metal material surface electroslag quick-equally heating and combination equipment. It is formed from equally-heating device, melting device and intercommunication device. It is characterized by that said intercommunication device is respectively connected with melting device and equally-heating device. Said invention is simple in structure, and can be used for producing various composite workpieces with several shapes.

The invention provides a noninvasive biopsy virus detection method based on high throughput gene sequencing. The method can carry out DNA sequencing and RNA sequence in parallel and thus precisely determines the biological composition and structure of viruses in patients. The method can maximally avoid the interference of nucleic acid of host cells, effectively reduce the cost of sequencing, and is capable of detecting multiple unknown crossed viruses at the same time. 80 DNA tagged connectors are designed, related reagents are assembled to form a library so as to construct a kit, and if the throughput allows, the kit can perform sequencing on 80 samples in parallel. The experiment design is strict, the automation degree is high, the virus identification can be finished in batches within 24 hours, the requirements of scientific research and clinical detection can be met in different levels, and references are provided for clinical diagnosis and prescription of diseases related with virus infection.

The invention relates to an adamantine chemiluminiscence kit for detecting a mechano-growth factor and the E peptide thereof and a manufacturing method of the kit. The prepared kit comprises streptavidin-coupled magnetic particle working fluid, biotin-labeled MGF and/or MGF-Ct24E antibody working fluid, alkaline phosphatase-labeled MGF and/or MGF-Ct24E antibody working fluid, MGF and MGF-Ct24E calibration product and/or quality control product working fluid, a chemiluminiscence substrate solution containing 3-(2-spiramantane)-4-methoxyl-4-(3-phosphorus oxygen acyl)-phenyl-1,2-dioxane disodiumsalt and cleaning fluid. A chemiluminescent system of the kit realizes chemiluminiscence through alkaline phosphatase/3-(2-spiramantane)-4-methoxyl-4-(3-phosphorus oxygen acyl)-phenyl-1,2-dioxane disodium salt; by use of a streptavidin-biotin signal amplification system, the kit is high in detection sensitivity, wide in linear range and high in detection result repetitiveness, and can realize accurate quantification of the mechano-growth factor and the E peptide thereof.

The invention relates to a method for detecting EDTA (ethylene diamine tetraacetic acid) in a laundry detergent. The method comprises steps as follows: (1) preparing a potassium bicarbonate aqueous solution; (2) adding the to-be-detected laundry detergent to the potassium bicarbonate aqueous solution, stirring the solution at the temperature of 45-50 DEG C for 10-20 min, filtering the solution while the solution is hot, and naturally cooling the solution to the room temperature to obtain a pretreated solution; (3) adding an iron liquid to the pretreated solution, performing oscillating extraction and then centrifugation, and removing an aqueous phase to obtain an ion liquid phase; (4) adding trivalent iron salt to the ion liquid phase, after full and even mixing, performing chromatography separation with a gel column chromatography Superose 12 column, performing washing with n-hexane firstly, then performing gradient elution with a petroleum ether-normal propyl alcohol mixed solvent serving as an eluent, and collecting the eluent; (5) evaporation the solvent of the eluent until the solvent is almost dry, and performing dissolution, volume determination and filtration with an organic membrane to obtain a detection sample; (6) performing liquid chromatography separation on the to-be-detected sample to determine the content. The method is excellent in EDTA quantitation accuracy, and a new detection means is provided for control on the content of the EDTA in the laundry detergent.

The inventio discloses an interleukin-6 detection kit. The kit includes a reagent R1 and a reagent R2; the reagent R1 includes a first buffer solution, a sealing agent and inorganic salt; and the reagent R2 includes a second buffer solution, streptavidin-modified microspheres, a biotin-labeled interleukin-6 monoclonal antibody or a biotin-labeled interleukin-6 polyclonal antibody, the sealing agent and carbohydrate. Through the introduction of a streptavidin-biotin signal amplification system, improvement can be performed on traditional latex enhanced immunoturbidimetry, detection sensitivity can be greatly enhanced, and the accurate quantification on interleukin-6 can be realized.

The invention discloses a PIK3CA gene mutation fluorescence quantitative PCR genotype detection kit comprising a PCR mixed reaction liquid, a positive control, and a fluorescent probe used for detecting PIK3CA gene mutant genotypes. The PCR mixed reaction liquid comprises PCR primers used for amplifying PIK3CA gene segments where the mutation sites exist. The invention also discloses a PIK3CA gene mutation fluorescence quantitative PCR genotype detection method. According to the method, PIK3CA gene mutant genotypes are subjected to fluorescence quantitative PCR detections by using the kit provided by the invention. With the technical scheme provided by the invention, detections can be carried out upon tissue PIK3CA gene mutations, and especially rapid, accurate, and high-sensitively detections upon PIK3CA gene 542,545 and 1047 nucleotide mutations can be realized.

The invention relates to an in vitro detection method of an antibody. An antibody to be detected can be determined by using an antigen with more than two antibody recognition epitopes to simultaneously combine with the antibody to be detected and the antibody with a marker to directly detect the marker or detect the marker after adding a reaction substrate. The detection result is consistent with the cognition habit of people, the in vitro detection method is more convenient to operate and judge. With the method, the sensitivity and the repeatability of the detection are improved and the detection specificity is enhanced.

The invention discloses a method for detecting homopiperazine in fasudil hydrochloride, which uses gas chromatography to qualitatively or quantitatively detect the homopiperazine in the fasudil hydrochloride. The diluent used in the sampling detection solution in the gas chromatography comprises DBU and organic solvent. According to the method for detecting the homopiperazine in the fasudil hydrochloride, the homopiperazine in the fasudil hydrochloride can be detected effectively and veritably, and the quality and the safety of fasudil hydrochloride are guaranteed.

The invention belongs to the technical field of element analysis and detection and particularly relates to a photogenic X-ray energy spectrum analyzer for rapidly measuring trace light elements and ananalysis method thereof. The energy spectrum analyzer comprises a cabinet (101), a computer (102), a sample chamber (303), an x-ray light pipe (301), a detector (302), a sample cup (305), a high-voltage power supply (306), a lifting platform (201) and a vacuum pump. The analysis method comprises the following steps: putting a to-be-detected sample (501) into the sample cup (305), wherein the surface of the to-be-detected sample (501) is bright and clean; lifting the sample cup (305) upwards through controlling the lifting platform (201) by means of the computer (102), placing the to-be-detected sample (501) at a detection position in the sample chamber (303), and quickly vacuumizing the sample chamber (303) by means of an oil-free vacuum pump. In this way, multiple trace amount of light elements can be measured at the same time and the detection limit of each element can be as low as 1 ppm. According to the invention, the absorption of air to light element characteristic spectrum peaks can be greatly reduced and the detection limit of light elements is reduced. The whole test is free of consumable materials, and has the characteristics of simple operation, lossless and fast detection, accurate determination, low cost and the like.

The invention relates to the field of detection, in particular to a method for quantitatively detecting melittin by liquid chromatography-tandem mass spectrometry, which comprises the step of detecting a characteristic peptide fragment of melittin in a sample to be detected by liquid chromatography-tandem mass spectrometry, wherein the sequence of the characteristic peptide fragment is VLTTGLPALISWIK. The method has high accuracy, precision and sensitivity, is high in stability, is suitable for accurate quantification of melittin in complex matrixes, and has important practical significance for protecting rights and interests of melittin consumers and maintaining healthy development of the bee product industry.

The invention discloses a system and a method for separating fluorine ions and low-molecular-weight organic acids by an ion-chromatographic-column switching method. The system comprises a sample injector, a six-way valve, a six-way valve switcher, an ion-exclusion column, a receiving pipe, a negative-ion exchange column, a liquid conveying pump, an electric-conductivity detector and a waste-liquid bottle, wherein the sample injector is connected with the six-way valve switcher which is connected with a liquid-phase pump and the ion-exclusion column; the six-way valve is connected with the receiving pipe, the liquid conveying pump and the negative-ion exchange column; and then the negative-ion exchange column is connected with a suppressor, the electric-conductivity detector and the waste-liquid bottle in sequence. The system and the method disclosed by the invention have the advantages that by use of the in-series connection of the conventional negative-ion exchange column and the ion-exclusion column, accurate quantification of the fluorine ions can be realized, the matrix interference in determination is reduced, online pretreatment of samples is realized and the artificial error and the manual labor are reduced; and the problem of interference of organic acids when conventional ion chromatography is used for determining the fluorine ions currently is solved and the independence of detection on the chromatographic column is reduced.

The invention provides a universal sample introduction apparatus and universal sample introduction method for gaseous hydrocarbons and liquefied petroleum gas. The universal sample introduction apparatus for gaseous hydrocarbons and liquefied petroleum gas comprises a sample introduction port (1), a sample flow control valve (2), a sample vaporizing chamber (3), a gas pressure reducing valve (4),a channel switching part (5), a gas quantification ring (6), a carrier gas inlet (7), a carrier gas flowmeter (8), an exhaust outlet (9), a counterbalance valve (10), an analyzer inlet (11) and a heating control module (12). The apparatus and method of the invention can realize accurate quantification of a sample; the counterbalance valve additionally arranged at the tail end of the quantificationring can guarantee stability and accuracy of pressure in the quantification ring, and sample sizes are maintained consistent every time through temperature control of the quantification ring, heat preservation of pipelines and passivation; and the apparatus can realize accurate quantification of the sample by increasing system pressure, so samples hard to detect or trace samples can be determined.

The invention relates to a sample injecting method, comprising the step of injecting a sample and a reagent into a sample injecting pipeline. The reagent comprises a carrying current, the carrying current pushes the sample out from the sample injecting pipeline, and the sample enters into a downstream sample injecting port. The invention also provides a water quality analyzing method and device. The invention has the advantages of accurate sample injecting and simple operation.

The invention provides a method for detecting carmustat mesylate related substances by adopting high performance liquid chromatography. A chromatographic column is an octadecylsilane chemically bondedsilica chromatographic column, and a mobile phase A is a mixed solution of sodium heptanesulfonate and trifluoroacetic acid; a mobile phase B is any one of acetonitrile, methanol, ethanol and tetrahydrofuran or a mixed solution of two of acetonitrile, methanol, ethanol and tetrahydrofuran; a diluent is pure water or a mixed solution of pure water and any one or two of methanol, acetonitrile, ethanol and tetrahydrofuran. The method can be applied to determination of carmustat mesylate related substances and determination of residual quantity in drugs using 4-dimethylaminopyridine as a catalystand N, N-dimethylformamide as a refining solvent in a synthesis process.

The invention relates to a method for quantitative analysis of multicomponent-adulterated pseudo-ginseng based on near infrared spectroscopy. The method comprises scanning an adulterated sample through a near infrared spectrometer to obtain a near infrared spectrum, respectively inspecting prediction accuracy and operation efficiency of principal component regression (PCR), support vector regression (SVR), partial least squares regression (PLSR), artificial neural network (ANN) and extreme learning machine (ELM) models, building an optimal modeling method and carrying out prediction on the content of the unknown sample through the optimal model. The method adopts a near infrared spectroscopy technology, is environmentally friendly and nondestructive and realizes fast detection. Through multiple multicomponent correction technologies and use of the optimal method, high accuracy is obtained. The method is suitable for quantitative adulteration of multicomponent-adulterated pseudo-ginseng.

The invention provides a high-flux integrated phosphorylated proteomics detection method. A capture skeleton is formed by a probe connected to a streptavidin plate and bait protein, a tyrosine phosphorylated protein compound is enriched, captured and subjected to enzymolysis to obtain polypeptide, and the sensitivity is improved by 300 times under the condition that the use amount of the probe isreduced by 100 times. Short-gradient rapid separation is realized by utilizing high performance liquid chromatography of a self-filling chromatographic column with the inner diameter of 150 microns; efficient detection is carried out in a DIA data acquisition mode by combining a Q Extractive HF-X mass spectrometer, and separation and detection of a single sample within 35 minutes can be realized under the condition of not losing qualitative and quantitative detection performance. Identification of transient changes of phosphorylation conditions of related signal channel proteins in cells underthe action of inhibitors with different concentrations in different EGF stimulation time is realized. And a high-flux, high-sensitivity and rapid detection method is provided for enrichment and identification of the tyrosine phosphorylated protein compound.

The invention relates to the field of image definition recognition, and discloses a logging image definition recognition method and device, a medium and electronic equipment. The method comprises the following steps: establishing a logging image sample library comprising a plurality of logging images; acquiring actual definition information corresponding to each logging image; acquiring a plurality of definitions corresponding to each logging image; determining a target weight corresponding to each target image definition determination algorithm according to the plurality of definitions corresponding to each logging image and the actual definition information; and determining the definition of the target logging image by using the target weight corresponding to each target image definition determination algorithm and each target image definition determination algorithm. According to the method, different advantages of various image definition determination algorithms are integrated, so that the method capable of accurately identifying the definition of the logging image is formed, and accurate quantification of the definition of the logging image can be realized.

The invention discloses a method for determining the content of total selenium in selenium-enriched proteoglycan by high performance liquid chromatography. The invention relates to a method for producing 2, 3, 5, 6-tetramethylpiperidine, 2, 3-diaminonaphthalene is used as a chelating agent; 400 microliters of acetonitrile is used as a dispersing agent; the method comprises the following steps: diluting a chelate (4, 5-benzo kohlrabi selenium brain) of selenium (IV) and 2, 3-diaminonaphthalene by acetonitrile, directly separating by using a PFP chromatographic column, eluting by using acetonitrile, measuring the fluorescence intensity by using a fluorescence detector under the conditions that the excitation wavelength is 376 nm and the emission wavelength is 520 nm, and quantifying by usingan external standard method. The method has the characteristics of stable experimental result, less sample transfer, less reagent consumption, high recovery rate and the like.

The invention relates to an in vitro detection method of a hepatitis B virus C antibody. A hepatitis B virus C antigen with more than two antibody recognition epitopes is used for simultaneously combining an antibody to be detected with an antibody combined with a label, and the antibody to be detected can be determined by directly detecting the label or detecting after a reaction substrate is added. The detection result is consistent to the recognition habit of human and is more convenient to operate and judge. The method also increases the sensitivity and the repeatability of detection and enhances detection specificity.

The invention provides a detonator automatic chemical dispensing system. The detonator automatic chemical dispensing system comprises a worktable, a man-machine interaction module, one or more chemical dispensing modules, a dosage control module, a stepping module and a control module, wherein the worktable is used for fixing detonator ignition parts to be subjected to chemical dispensing; the man-machine interaction module is used for setting and displaying working parameters and a working process flow of the chemical dispensing system; one or more chemical dispensing modules are used for performing chemical dispensing on the detonator ignition parts; the dosage control module is used for controlling the single chemical dispensing amount of each chemical dispensing module; the stepping module is used for controlling the relative movement of each chemical dispensing module and the worktable to enable each chemical dispensing module to dispense chemicals into the detonator ignition part; and the control module is used for controlling the working of the chemical dispensing modules, the dosage control module and the stepping module according to the working parameters and the working process flow. The chemical dispensing system realizes the accurate positioning and the precise quantification of chemical dispensing operation; automated batch chemical dispensing of the detonator ignition parts is realized, thereby being conductive to improving production efficiency; and by controlling the single chemical dispensing amount, the amount of the chemicals dispensed into the ignition parts is kept consistent, thereby being conductive to improving the consistency of the ignition parts, the product quality of the detonator ignition parts and the qualification rate of finished products.