201 results about "Control sample" patented technology

The invention discloses a triaxial acoustic emission test device and method for multi-field coupling rock microbial grouting. The triaxial acoustic emission test device comprises a core triaxial pressure chamber, a freezing and thawing temperature control system, a water pressure control system, an axial loading system, an acoustic emission detection system, a microbial grouting repair device and an assembly data acquisition and control system, the test sample temperature is controlled through the freeze-thaw temperature control system, the pore water pressure is applied through the water pressure control system, the mechanical load is applied through the axial loading system, the damage signal of the test sample is monitored in real time through the acoustic emission system, the test sample is prepared through the microorganism grouting repair device, and the assembly data acquisition and control system performs integrated control and synchronous data acquisition. The test method comprises the following steps: preparing and installing a microbial remediation rock sample, applying freeze-thaw cycle, pore water pressure and axial load in sequence or in a coupling manner, monitoring the whole process by using an acoustic emission technology, and finally comprehensively analyzing a damage evolution rule and a performance degradation mechanism of the sample under the action of multi-field coupling. The device can simulate a complex environment and evaluate the microbial grouting repair effect.

The invention discloses a high-flux and rapid chromatin immunoprecipitation sequencing method based on a HiBiT tag and a Tn5 enzyme, and belongs to the technical field of biology. According to the method, the high affinity characteristic of a HiBiT tag system is combined with a Tn5 enzyme in-situ library building technology, firstly, a DNA binding protein recombinant expression strain containing a HiBiT tag is constructed and subjected to induced expression, after formaldehyde crosslinking and cell lysis, an Anti-HiBiT antibody is used for rapid immunoprecipitation, and a Tn5 enzyme library is constructed; tn5 transposase is directly loaded on the magnetic beads combined with the protein and DNA fragment compound to complete fragmentation and linker connection, so that the construction of a next-generation sequencing library is quickly carried out, and the total consumed time from immunoprecipitation to the completion of library construction is less than or equal to 5 hours. Meanwhile, automatic treatment is realized through a micro experiment board (the reaction system is less than or equal to 200 microliters) and a liquid workstation, the experiment cost and the sample dosage are reduced, 48 transcription factors and 48 control samples can be synchronously treated, the efficiency is greatly improved, and an efficient tool is provided for microbial transcription regulation and control research.

The invention discloses a determination method of an Ajowan fruit fingerprint spectrum and the fingerprint spectrum thereof, and relates to the technical field of fingerprint spectrums of medicinal materials. The method comprises the following steps: taking rutin, caffeic acid, rosmarinic acid and p-coumaric acid as reference substances, dissolving the reference substances in a solvent, and fixing the volume to obtain a reference substance solution; the method comprises the following steps: taking different batches of ajowan fruit medicinal materials as test products, adding a solvent into the test products, extracting, filtering, and taking subsequent filtrate to obtain a test product solution; respectively injecting the reference substance solution and the test solution into a liquid chromatograph by utilizing a high performance liquid chromatography, recording a chromatogram, and constructing the fingerprint spectrum of the Ajowan fruit according to the chromatogram; and determining the component content of the test solution through an external standard method according to the chromatogram. According to the method, the quality of the ajowan fruit medicinal material is controlled by adopting a method of combining chemical component content determination and a fingerprint spectrum, so that the effective and comprehensive control on the ajowan fruit medicinal material is realized, and a basis is provided for the quality control standard of the ajowan fruit medicinal material.

The invention discloses a method for detecting anions in a phlegm-heat-clearing injection, the detection method comprises the following steps: a test solution and a reference solution are taken for detection, the phlegm-heat-clearing injection is composed of scutellaria baicalensis, bear gall powder, cornu gorais, honeysuckle, fructus forsythiae and propylene glycol; chromatographic conditions of the detection are as follows: an anion exchange chromatographic column is adopted, an eluent is an acid aqueous solution, an alkali aqueous solution and / or a buffer salt aqueous solution, the flow velocity is 0.1-2.0 ml / min, the column temperature is 20-40 DEG C, a detector is an electrical conductivity detector, the detection mode is suppression electrical conductivity detection, and the sample size is 10-40 [mu] l; and obtaining the content information of the anions in the phlegm-heat clearing injection according to the detection result. The method disclosed by the invention is simple, convenient, efficient and rapid, and provides a theoretical basis for the quality control of the phlegm-heat clearing injection and the deep research of a pharmacodynamic material basis by detecting the content of the anions.

The application belongs to the technical field of material detection, and specifically provides a method for determining the sodium expansion rate of an aluminum cathode with a non-standard size under pressure, characterized in that the method comprises the following steps: preparing a composite sample through an aluminum cathode carbon block and a graphite block, and preparing a control sample through the graphite block; electrolyzing the composite sample and the control sample respectively; determining a first length increment curve of the composite sample in an electrolysis process, and determining a second length increment curve of the control sample in the electrolysis process; and determining the sodium expansion rate of the aluminum cathode based on the first length increment curve and the second length increment curve. The technical scheme provided in the application can realize the determination of the sodium expansion rate of an aluminum cathode with a non-standard size.

The invention discloses a PLC-based rectifying tower parameter automatic adjustment method and system, and belongs to the technical field of rectifying tower control. The method comprises the steps of performing clustering processing on historical regulation and control parameters to construct a classification area, constructing regulation and control samples marked as primary samples and secondary samples based on the classification area, and a sample intelligent model, determining target regulation and control parameters based on the primary samples, and transmitting the target regulation and control parameters to a PLC control system to execute regulation and control actions, and acquiring real-time operation parameters of the rectifying tower, inputting the real-time operation parameters into the sample intelligent model to output a simulation regulation and control parameter set, further judging regulation and control feedback, and triggering updating of the sample intelligent model when the regulation and control feedback is in a deviation state. According to the method, through double screening of historical regulation and control parameters and simulation pre-judgment before regulation and control, the accuracy of the regulation and control parameters is improved, and the stability, safety and robustness of the system are enhanced through closed-loop self-learning and fault-tolerant mechanisms.

This invention discloses a quantitative method for phospholipids, comprising: a sample preparation step, wherein a sample containing protein and phospholipids is mixed with Schiff reagent to obtain a sample for testing; a control sample preparation step, wherein the sample is mixed with a solvent of Schiff reagent to obtain a control sample; a measurement step, wherein the degree of a first optical property of the sample for testing and the degree of an optical property of the control sample are measured after the reaction of the aldehyde formed by the vinyl ether bond of phospholipids with Schiff reagent; and a determination step, wherein a correction degree obtained by correcting the degree of the first optical property using the degree of the optical property of the control sample is compared with the degree of a second optical property of the standard sample to determine the concentration of phospholipids in the sample, wherein the standard sample contains Schiff reagent and phospholipids of known concentration, and the degree of the second optical property is the degree of reaction of the Schiff reagent with the aldehyde formed by the vinyl ether bond of phospholipids.

The invention discloses a method for determining related substances in a loxoprofen sodium patch in the technical field of drug detection, and aims to solve the technical blank existing in determination of related substances in a loxoprofen sodium patch in the prior art. The method comprises the following steps: respectively weighing a loxoprofen sodium reference substance and an impurity reference substance, and adding a solvent for dilution to prepare a reference substance solution; taking a loxoprofen sodium patch, stripping a protective layer, adding tetrahydrofuran and methanol, shaking to separate the paste from a backing layer, adding water, shaking again, adding a solvent for dilution, uniformly mixing, centrifuging, and taking supernate to obtain a test solution; injecting the prepared reference substance solution and the test solution into a high performance liquid chromatograph for determination to obtain peak areas of the reference substance solution and the test solution; and calculating the content of the related substances in the loxoprofen sodium patch by adopting an HPLC correction factor method according to the peak areas of the reference solution and the test solution. The method is suitable for measuring the content of the related substances in the loxoprofen sodium patch.

The invention discloses a rapid detection method for talcum powder in food, and relates to the technical field of element analysis and detection. The detection method comprises the following steps: uniformly mixing a to-be-detected sample with nitric acid, perchloric acid and sulfuric acid, heating and digesting, supplementing nitric acid and continuing digesting, and increasing the heating and digesting temperature until the to-be-detected sample is completely digesting to obtain a digesting solution; adding the digestion solution into a colorimetric tube, adding water to a scale, and uniformly shaking to obtain a to-be-detected sample colorimetric solution; mixing another to-be-detected sample with the same mass with talcum powder to obtain a control sample, and treating according to the same method to obtain a control sample colorimetric solution; performing oscillation treatment on the to-be-detected sample colorimetric solution and the control sample colorimetric solution, and performing turbidity comparison under a black background through turbidimetry to obtain a detection result. The method is quick and simple to operate, high in sensitivity, high in accuracy, safe, efficient and energy-saving, the detection period is shortened by half compared with that of a national standard method, and the talcum powder in batch food can be detected at the same time.

The application discloses an infrared small target detection network training method and device, equipment and a storage medium, and relates to the technical field of target detection. The method comprises the following steps: based on the number of positive samples and a preset proportion coefficient, difficult negative sample sets and easy negative sample sets are selected from an initial negative sample set; the preset proportion coefficient is a coefficient that is preset to improve the balance degree of the number of positive and negative samples; a weight matrix for controlling samples in a back propagation process is generated according to the difficult negative sample sets and the easy negative sample sets; a difficult point mining loss function is determined based on the weight matrix, and the infrared small target detection network is point supervised and trained by using the difficult point mining loss function. Through the above scheme, the difficult negative sample sets and the easy negative sample sets are determined based on the number of positive samples and the preset proportion coefficient, the unbalanced situation of the number of positive and negative samples in the target detection network training process is prevented, the balance degree of the number of positive and negative samples is improved, and therefore the training effect of the target detection network is improved.

Disclosed are methods of predicting a. subject's responsiveness to an immunotherapy comprising detecting the presence and / or amount of a miR155 gene signature in the subject or a sample from the subject, wherein the miR155 gene signature comprises one or more of miR155, CD3E, CD3G, CD8A, CD8B, CXCR6, FXYD5, GZMB, ID2, IFNgamma, LAGS, NKG7, PDCD1, S100A4, and TIGIT; and comparing the presence and / or amount of the miR155 gene signature to a control sample or threshold, wherein the presence and / or an altered amount of the miR155 gene signature relative to the presence or amount in the control sample or threshold indicates the subject will be responsive or is responding to the immunotherapy.

The invention discloses a fingerprint spectrum of Huanglong cough-relieving granules and a construction method, and relates to the technical field of pharmaceutical analysis. The construction method comprises the following steps: (1) preparation of a test solution: taking Huanglong cough-relieving granules, and adding an extraction solvent for extraction; (2) preparing a reference substance solution; (3) preparing a reference medicinal material solution; (4) chromatographic conditions: octadecylsilane chemically bonded silica is used as a filler; the mobile phase A is acetonitrile, the mobile phase B is 0.1-0.2% phosphoric acid, the flow rate is 0.38-0.42 ml / min, the column temperature is 29-31 DEG C, the detection wavelength is 210 nm in 0-17 min, and the detection wavelength is 270 nm in 17-48 min; and (5) absorbing the solution, and adding the solution into a liquid chromatograph for detection. The fingerprint spectrum constructed by the method can systematically reflect the material basis, is relatively good in precision, repeatability and stability, and can be used for quality control of the Huanglong cough-relieving granules.

The invention relates to a detection method, in particular to a method for detecting aldehyde impurities in polysorbate 20. According to the method, interference caused by the purity of 2, 4-dinitrophenylhydrazine is avoided, derivatization of a reference substance is performed by adopting a method synchronously operating with a test sample, eight aldehyde ketone substances can be detected at the same time, and compared with literature methods, the method is high in specificity, good in precision, high in accuracy, simple and easy to implement and high in operability.

This invention discloses a simulated oxidation detection device and method for lead-acid battery casings, including a heating furnace, a beaker placed on the furnace, and an adjusting bracket placed on the side of the furnace. One end of the adjusting bracket extends above the beaker, and a temperature measuring element and a sample are connected to the adjusting bracket, with the temperature measuring element and the sample immersed in cooling water. During detection, the heating furnace is turned on, and cooling water from a workshop water bath is placed in the beaker, with the internal water temperature heated to a stable 40℃±1℃. Two prepared samples are taken: sample A is wrapped with a PE protective film, and sample B is without a film. They are connected to the bracket, with one end immersed in water, and continuously bathed for 24 hours. Finally, the two samples are compared with a control sample. Compared with existing technologies, this invention has a scientifically sound structure, is simple and convenient to operate, and can screen out batches of ABS granules with poor acid resistance and heat resistance at the material stage, preventing them from flowing into subsequent injection molding and battery assembly processes, thus eliminating the phenomenon of discoloration and mottling of the casing from the source.

This application relates to the field of biomedical technology, and in particular to a method for evaluating the effectiveness of autism intervention and its application. The method includes the following steps: collecting a test sample and a control sample; the test sample is a urine sample from an autism patient, and the control sample is a urine sample from a healthy volunteer; processing the test sample and the control sample separately to obtain NMR samples; testing the NMR samples using an NMR spectrometer to obtain NMR spectra; performing multivariate statistical analysis on the NMR spectra to construct a urinary metabolite model; and using the urinary metabolite model to screen and evaluate autism patients. This application provides a method for evaluating the effectiveness of autism intervention and its application, constructing a urinary metabolite model through NMR metabolomics technology, providing practical evidence for establishing methods for early screening and treatment process evaluation of autism.

The utility model discloses a kind of sample falling devices, including supporting mechanism, sample falling mechanism, push sample mechanism and sample inlet mechanism, sample falling mechanism includes the baffle ring disc fixed on supporting mechanism, sample falling disc is coaxially arranged with baffle ring disc and rotatably installed on baffle ring disc, and drive assembly for controlling sample falling disc rotation along circumference is installed on supporting mechanism, the end face of sample falling disc is spaced apart and is equipped with several toothed plates along circumference, and the baffle ring disc between adjacent two toothed plates forms the sample falling groove for accommodating sample ball;Push sample mechanism includes sample receiving assembly for receiving sample ball and being arranged on the outer circumferential side of baffle ring disc, and push sample assembly is installed on baffle ring disc and is used to push sample ball in sample falling groove along the radial direction of baffle ring disc and enter sample receiving assembly;Sample inlet mechanism is installed on baffle ring disc and is used to correspond to be arranged above sample falling groove to transmit sample ball into sample falling groove.The device can control the drop of sample ball, sample falling disc can be positioned and sample is sent, and efficiency is high, and sample ball will not be damaged by friction.

The application discloses a method for screening a quality marker of Radix Paeoniae Rubra based on an anticoagulation spectrum-effect relationship, mainly comprising the following steps: preparing control samples and Radix Paeoniae Rubra sample materials, determining a preparation method of the Radix Paeoniae Rubra sample and optimal chromatographic conditions for separating the Radix Paeoniae Rubra sample, and constructing a fingerprint of the Radix Paeoniae Rubra sample; using a blood coagulation instrument to determine an in-vitro anticoagulation activity of Radix Paeoniae Rubra extracts; analyzing experimental data to determine the quality marker of Radix Paeoniae Rubra based on the anticoagulation spectrum-effect relationship. The efficacy and common peaks of the fingerprint are analyzed by a grey correlation degree analysis method, and the quality marker of Radix Paeoniae Rubra for anticoagulation is screened. The method overcomes the fact that the prior art does not have a related technology for determining the quality marker of Radix Paeoniae Rubra for anticoagulation by the grey correlation degree analysis method.

The invention discloses an irradiation hardening coefficient generation method and an irradiation hardening evaluation method, and relates to the technical field of irradiation hardening. The coefficient generation method comprises the following steps: performing ion irradiation on a test sample, and simulating according to ion irradiation parameters to obtain an irradiation damage depth distribution map; performing nanoindentation test on the test sample subjected to ion irradiation and a control sample of the test sample to obtain a first load displacement curve of the test sample and a second load displacement curve of the control sample; obtaining first and second simulated load displacement curves according to the first and second load displacement curves; obtaining a first elastic-plastic transition point and a second elastic-plastic transition point according to the first load displacement curve, the first simulated load displacement curve, the second load displacement curve and the second simulated load displacement curve; and obtaining the irradiation hardening coefficient according to the first elastic-plastic transition point, the second elastic-plastic transition point and the irradiation damage depth distribution map. Therefore, the radiation hardening can be quantitatively evaluated.

The invention discloses a method for detecting the content of formaldehyde in polyethylene glycol 400, and relates to the technical field of detection. The invention relates to a method for detecting the content of formaldehyde in polyethylene glycol 400, which comprises the following steps: S1, precisely weighing 4-(3-methylphenyl)-2H-pyridine [4, 3, e]-1, 2, 4-thiodiazine-1, 1-dioxo, and adding methanol to dissolve the 4-(3-methylphenyl)-2H-pyridine [4, 3, e]-1, 2, 4-thiodiazine-1, 1-dioxo to prepare a reference solution; s2, precisely weighing polyethylene glycol 400 and reactants to prepare a test solution; and S3, respectively detecting the test solution and the reference solution by adopting a high performance liquid chromatography, and calculating the content of formaldehyde in polyethylene glycol 400, wherein the chromatographic conditions of the high performance liquid chromatography are as follows: gradient elution is adopted; a chromatographic column is characterized in that octadecylsilane chemically bonded silica is used as a stationary phase; the mobile phase comprises a first mobile phase and a second mobile phase, the first mobile phase is a buffer solution, and the second mobile phase is an organic solvent. The method provided by the invention can accurately measure the content of formaldehyde in polyethylene glycol 400.

The present disclosure provides a MNP marker primer combination for macadamia variety identification, a kit and its application. The primer combination comprises: 1st-446th primer pairs, forward primer of 1st primer pair, reverse primer of 1st primer pair to forward primer of 446th primer pair and reverse primer of 446th primer pair, which are sequentially shown in SEQ ID NO: 1-SEQ ID NO: 892 in the sequence listing. Using the MNP marker primer combination, DNA fingerprint data of the sample to be tested can be obtained by multiplex PCR amplification and high-throughput sequencing, and then compared with the control sample to obtain the genetic similarity coefficient, and finally obtain the variety identification conclusion of the sample to be tested and the control sample, and the accuracy and digitization degree are both high. A sequence analysis software can be used to compare hundreds of samples to be tested at one time, and the variety identification conclusion can be quickly obtained, which can significantly improve the accuracy and efficiency of macadamia variety identification.

A DMA replication-associated protein (Rep) for multiple sclerosis (MS) is described, wherein (a) an increased amount of Rep protein or fragments thereof in a sample from a subject compared to an amount in a control sample or an increased amount of anti-Rep protein antibodies to antigen in a sample from a subject compared to an amount in a control sample is associated with a diagnosis of MS, wherein the Rep protein is an MSBI1 Rep or MSBI2 Rep.

The invention provides a method for detecting amylase in oyster sauce, which is based on configuration and culture of a buffer solution, a blank control sample and a to-be-detected sample, and finally by observing starch paste states of the to-be-detected sample and the control sample, whether the starch paste state of the to-be-detected sample is thinned is observed, and then whether the to-be-detected sample contains amylase is judged. The method can intuitively and efficiently detect whether the raw material contains amylase, and avoids the technical problems that the color of the oyster sauce finished product interferes with the detection result, the degradation effect of microorganisms on starch causes'false positive 'and the acid degradation effect of acidic raw materials on starch causes'false positive' in the prior art. The oyster sauce finished product is fully prevented from being thinned in the shelf life, and the product quality is ensured.

The application discloses a method for detecting 5-hydroxymethylfurfural in a compatibility experiment of hydrocortisone sodium succinate for injection and glucose injection by HPLC. The 5-hydroxymethylfurfural is a degradation impurity of glucose. The detection method comprises the following steps: (1) preparation of a control sample solution; (2) preparation of a test sample solution; (3) chromatographic conditions; a mobile phase A is 8mmol / L potassium dihydrogen phosphate solution (pH value is adjusted to 5.0-5.1 by using 8mmol / L potassium hydrogen phosphate solution, and the solution is prepared freshly before use); a mobile phase B is methanol, gradient elution is carried out; a detection wavelength is 284nm; a column temperature is 40 DEG C; a flow rate is 1.4-1.6mL / min; and a chromatographic column is C 18 ; the application can effectively detect the content of 5-hydroxymethylfurfural, and has strong specificity and high sensitivity.

This invention provides a chromosome copy number counting method based on the relative fluorescence intensity between chromosome monosoms. This method leverages the built-in genetic markers of different chromosomes in multiplex STR typing systems. By introducing a control sample with known ploidy, and considering that the relative fluorescence intensity between two chromosome monosoms is a constant value, the ploidy of chromosomes with unknown ploidy in the test sample is calculated. This method yields accurate results that can be cross-validated with ploidy determinations based on genetic marker typing, eliminating the need for further screening of other polymorphic genetic markers or additional experimental studies to determine the ploidy of the chromosome. Furthermore, it can serve as a basis for developing corresponding software for multiplex STR typing systems. Developing corresponding analytical software based on this calculation scheme can significantly reduce manual intervention in data analysis, improve the accuracy of analytical results, and significantly reduce experimental workload.

This invention discloses a method for testing the crosslinking degree of hard carbon precursors and a method for predicting the capacity of hard carbon, belonging to the technical field of performance evaluation of battery anode materials. The method includes the following steps: preparing a control sample and a test sample of the precursor; collecting infrared spectral data of the control sample and the test sample under the same conditions; extracting the peak area of ​​characteristic functional groups in the infrared spectral curves using peak fitting; calculating the crosslinking degree of the hard carbon precursor according to a formula; verifying the accuracy of the detection method using conventional dynamic mechanical thermodynamic analysis; and establishing a mathematical relationship between the crosslinking degree of the precursor sample and the specific capacity of the derived hard carbon. This allows for the measurement of the crosslinking degree of the precursor sample and the prediction of the sodium storage capacity of the hard carbon anode material. Compared with existing technologies, this invention has stronger universality, simpler operation, quantitative analysis, and better data stability, making it an advanced detection technology for hard carbon anode materials.

The application provides a HPLC-based method for constructing a fingerprint spectrum of Tianyang Qianggu Yin and application thereof, and belongs to the technical field of traditional Chinese medicine detection.The method provided by the application comprises the steps of preparing a test sample solution, preparing a mixed control sample solution, HPLC determination, fingerprint spectrum construction and content determination.The application first establishes a HPLC fingerprint spectrum suitable for water-soluble extracts of Tianyang Qianggu Yin compound, and simultaneously realizes accurate content determination of six components, i.e., neotamanoxin, naringin, alpinumisine A, alpinumisine B, alpinumisine C and icariin.The application has strong specificity, high precision, good repeatability and excellent accuracy.The application provides scientific and controllable technical support for the production quality consistency and efficacy stability of Tianyang Qianggu Yin, and can be used for quality control and batch consistency evaluation of the compound preparation.

The invention relates to the technical field of medical analysis, in particular to a method for detecting the content of an antioxidant in bromfenac sodium eye drops through liquid chromatography, and particularly relates to a method for detecting the content of the antioxidant anhydrous sodium sulfite of the bromfenac sodium eye drops through high performance liquid chromatography. Wherein the mobile phase A is a methanol-buffer salt solution; a mobile phase B is methanol; eluting by using a gradient mobile phase, wherein the volume ratio of the mobile phase is 100%; preparing a reference solution and a test solution by taking the mobile phase A as a diluent, then respectively injecting the reference solution and the test solution into a liquid chromatograph, and recording chromatograms; calculating the content of anhydrous sodium sulfite by adopting an external standard method; the method comprises the following specific steps: (1) preparation of a reference substance solution: taking an anhydrous sodium sulfite reference substance, and adding a diluent for dissolving to obtain the reference substance solution; and (2) preparation of a test solution: taking the bromfenac sodium eye drops, and adding a diluent to dilute the bromfenac sodium eye drops to obtain the bromfenac sodium eye drops. The method disclosed by the invention is strong in specificity, high in stability, high in precision and high in accuracy, and has practical significance on quality control of the bromfenac sodium eye drops.

The application provides a method for determining the content of sandalwood medicinal materials, decoction pieces, standard decoction and formula granules, which takes verbascoside as a control sample, and detects the test sample solution and the control sample solution through liquid chromatography, and takes acetonitrile-water with a volume ratio of (15-20):(80-85) as a mobile phase. The method can scientifically identify sandalwood medicinal materials, decoction pieces, standard decoction and formula granules, can control the internal quality of sandalwood and related preparations from the whole and macro, and can effectively guarantee the curative effect of the medicine, so that the medicinal materials and related preparations can be more regularly controlled in quality.

The invention discloses a method for detecting the content of various components in a puffball sample and application of the method, and the method comprises the following steps: taking puffball sample powder, adding an extraction solvent for extraction, and preparing to obtain a test solution; taking alpha-linolenic acid, linoleic acid, palmitic acid, oleic acid, stearic acid and ergosterol reference substances, and adding a solvent to prepare a mixed reference substance solution; respectively injecting the mixed reference substance solution and the test solution into an ultra-high performance liquid chromatograph for detection, and comparing the maps of the reference substance solution and the test solution; and making a regression equation by using the sample size Ln value and the peak area Ln value of each component, and calculating the content of the components in the puffball sample. According to the quality detection method for the puffball sample, the contents of the six components can be simultaneously determined, the operation is simple, the accuracy is high, the recovery rate is reliable, the detection efficiency is high, and a basis is provided for standardizing the market of puffball medicinal materials and preparations and reasonably developing the puffball medicinal materials and preparations.

A training method of a marketing prediction model for eliminating time deviation and a precision marketing method relate to the technical field of data processing, samples are grouped by setting time stamps and marketing scheme stamps, experimental samples and contrast samples in the same time group are ensured to be comparable in average value, and the accuracy of marketing prediction is improved. Therefore, the time deviation problem when historical samples are adopted is eliminated, meanwhile, purchase rate target pre-training is carried out on the model to solve the problem that high-latitude features cannot be effectively utilized due to the fact that the unbiased sample size is small, and finally the overall loss is calculated in a multi-task training mode, so that the accuracy of the model is improved. The marketing prediction model can achieve better prediction accuracy under the condition that biased samples and a small number of unbiased samples are adopted, so that the sampling cost caused by only using the unbiased samples is greatly reduced, and both the cost and the effect are achieved. By using the marketing prediction model, accurate putting of marketing schemes can be further realized, and the marketing prediction model has a good practical value.