286 results about "Control sample" patented technology

The invention relates to a method for constructing HPLC (High Performance Liquid Chromatography) contents of multi-index components of a reference sample of traditional Chinese medicine powder, which comprises the following steps: step 1, preparation of a reference sample of Kaixin powder: taking polygala tenuifolia, ginseng, poria cocos and rhizoma acori graminei, and crushing into fine powder; 2, preparing a Kaixin powder reference sample and a reference substance detection solution; 3, detecting the Kaixin powder reference sample and the reference substance detection liquid by adopting an HPLC (High Performance Liquid Chromatography) instrument to obtain a chromatogram; and step 4, calculating the content of the effective components in the Kaixin powder reference sample according to the chromatogram. Wherein in the step 3, an HPLC (High Performance Liquid Chromatography) instrument is adopted for detection, and a chromatogram is obtained; comprising the following steps: step A, detecting the contents of polygala # imgabs0 # ketone III, 3, 6 '-dimustard acyl sucrose and beta-asarone in rhizoma acori graminei in polygala tenuifolia; step B, detecting the contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in the ginseng; and step C, detecting the content of tenuifolin in the polygala tenuifolia.

The invention discloses real-time monitoring sampling equipment for water environment ecological restoration and a sampling method thereof, and relates to the technical field of water environment sampling equipment.The real-time monitoring sampling equipment comprises a main shaft rod, a real-time data display screen arranged at the top of the main shaft rod, a ground insert fixed to the bottom of the main shaft rod and a floating plate arranged on the main shaft rod in a sleeving mode; the buoyancy driving structure is arranged on the main shaft rod in a sleeving manner and controls sampling state randomness based on rising of the floating plate; the random sampling structure is driven by a transmission assembly in the buoyancy driving structure to rotate for random sampling; the sensing detection assembly is in contact with a water sample to transmit water quality data; the buoyancy driving structure comprises a lifting plate fixed to the bottom of the floating plate, and a driving groove is formed in the lifting plate. By means of the water environment sampling device, the problem that the sampling range is limited to a certain extent due to the fact that most of layered water body sampling areas are fixed sampling positions and other water samples at the same water level cannot be sampled in water environment sampling equipment is solved.

The invention discloses a triaxial acoustic emission test device and method for multi-field coupling rock microbial grouting. The triaxial acoustic emission test device comprises a core triaxial pressure chamber, a freezing and thawing temperature control system, a water pressure control system, an axial loading system, an acoustic emission detection system, a microbial grouting repair device and an assembly data acquisition and control system, the test sample temperature is controlled through the freeze-thaw temperature control system, the pore water pressure is applied through the water pressure control system, the mechanical load is applied through the axial loading system, the damage signal of the test sample is monitored in real time through the acoustic emission system, the test sample is prepared through the microorganism grouting repair device, and the assembly data acquisition and control system performs integrated control and synchronous data acquisition. The test method comprises the following steps: preparing and installing a microbial remediation rock sample, applying freeze-thaw cycle, pore water pressure and axial load in sequence or in a coupling manner, monitoring the whole process by using an acoustic emission technology, and finally comprehensively analyzing a damage evolution rule and a performance degradation mechanism of the sample under the action of multi-field coupling. The device can simulate a complex environment and evaluate the microbial grouting repair effect.

The invention discloses a high-flux and rapid chromatin immunoprecipitation sequencing method based on a HiBiT tag and a Tn5 enzyme, and belongs to the technical field of biology. According to the method, the high affinity characteristic of a HiBiT tag system is combined with a Tn5 enzyme in-situ library building technology, firstly, a DNA binding protein recombinant expression strain containing a HiBiT tag is constructed and subjected to induced expression, after formaldehyde crosslinking and cell lysis, an Anti-HiBiT antibody is used for rapid immunoprecipitation, and a Tn5 enzyme library is constructed; tn5 transposase is directly loaded on the magnetic beads combined with the protein and DNA fragment compound to complete fragmentation and linker connection, so that the construction of a next-generation sequencing library is quickly carried out, and the total consumed time from immunoprecipitation to the completion of library construction is less than or equal to 5 hours. Meanwhile, automatic treatment is realized through a micro experiment board (the reaction system is less than or equal to 200 microliters) and a liquid workstation, the experiment cost and the sample dosage are reduced, 48 transcription factors and 48 control samples can be synchronously treated, the efficiency is greatly improved, and an efficient tool is provided for microbial transcription regulation and control research.

The present disclosure relates to the field of chemical analysis technology, and specifically discloses methods and systems for analyzing and calculating aggregation of analytes in a chemical compound. The analysis method includes: acquiring a chemical image of analytes in a control sample and a chemical image of the analytes in at least one test sample, performing Gaussian blur on each of the chemical images, and performing brightness calibration on the obtained Gaussian blurred image; converting the brightness calibrated Gaussian blurred image into a binary image; converting the binary image into one-dimensional array; calculating a continuous length of predetermined pixels in a one-dimensional array and a frequency corresponding to each continuous length, and generating a histogram of frequency and continuous length; fitting the histogram with an exponential Gaussian distribution function to obtain an exponential attenuation parameter of the fitted exponential Gaussian distribution function; calculating an aggregation index of the test sample according to the exponential attenuation parameters corresponding to the test sample and the control sample. This technical solution can quantitatively determine the aggregation of analytes.

Techniques are provided for generating an array-specific range of Tm values to be used for calling a sample in a given array positive or negative for a target nucleic acid sequence. A sample well in an array is provided with a control sample containing a control nucleic acid sequence. The control sample is amplified by thermal cycling the sample well. A Tm value for the control sample is identified and compared to an expected Tm value for the control nucleic acid sequence to calculate a relationship between the identified control Tm value and the expected control Tm value. By applying this relationship to an expected Tm value for a target nucleic acid sequence, an array-specific range of Tm values for the target nucleic acid sequence is generated and can be used for calling an experimental sample in the same array positive or negative for the target nucleic acid sequence.

The invention discloses a determination method of an Ajowan fruit fingerprint spectrum and the fingerprint spectrum thereof, and relates to the technical field of fingerprint spectrums of medicinal materials. The method comprises the following steps: taking rutin, caffeic acid, rosmarinic acid and p-coumaric acid as reference substances, dissolving the reference substances in a solvent, and fixing the volume to obtain a reference substance solution; the method comprises the following steps: taking different batches of ajowan fruit medicinal materials as test products, adding a solvent into the test products, extracting, filtering, and taking subsequent filtrate to obtain a test product solution; respectively injecting the reference substance solution and the test solution into a liquid chromatograph by utilizing a high performance liquid chromatography, recording a chromatogram, and constructing the fingerprint spectrum of the Ajowan fruit according to the chromatogram; and determining the component content of the test solution through an external standard method according to the chromatogram. According to the method, the quality of the ajowan fruit medicinal material is controlled by adopting a method of combining chemical component content determination and a fingerprint spectrum, so that the effective and comprehensive control on the ajowan fruit medicinal material is realized, and a basis is provided for the quality control standard of the ajowan fruit medicinal material.

The invention discloses a method for detecting anions in a phlegm-heat-clearing injection, the detection method comprises the following steps: a test solution and a reference solution are taken for detection, the phlegm-heat-clearing injection is composed of scutellaria baicalensis, bear gall powder, cornu gorais, honeysuckle, fructus forsythiae and propylene glycol; chromatographic conditions of the detection are as follows: an anion exchange chromatographic column is adopted, an eluent is an acid aqueous solution, an alkali aqueous solution and / or a buffer salt aqueous solution, the flow velocity is 0.1-2.0 ml / min, the column temperature is 20-40 DEG C, a detector is an electrical conductivity detector, the detection mode is suppression electrical conductivity detection, and the sample size is 10-40 [mu] l; and obtaining the content information of the anions in the phlegm-heat clearing injection according to the detection result. The method disclosed by the invention is simple, convenient, efficient and rapid, and provides a theoretical basis for the quality control of the phlegm-heat clearing injection and the deep research of a pharmacodynamic material basis by detecting the content of the anions.

The application belongs to the technical field of material detection, and specifically provides a method for determining the sodium expansion rate of an aluminum cathode with a non-standard size under pressure, characterized in that the method comprises the following steps: preparing a composite sample through an aluminum cathode carbon block and a graphite block, and preparing a control sample through the graphite block; electrolyzing the composite sample and the control sample respectively; determining a first length increment curve of the composite sample in an electrolysis process, and determining a second length increment curve of the control sample in the electrolysis process; and determining the sodium expansion rate of the aluminum cathode based on the first length increment curve and the second length increment curve. The technical scheme provided in the application can realize the determination of the sodium expansion rate of an aluminum cathode with a non-standard size.

The invention discloses a PLC-based rectifying tower parameter automatic adjustment method and system, and belongs to the technical field of rectifying tower control. The method comprises the steps of performing clustering processing on historical regulation and control parameters to construct a classification area, constructing regulation and control samples marked as primary samples and secondary samples based on the classification area, and a sample intelligent model, determining target regulation and control parameters based on the primary samples, and transmitting the target regulation and control parameters to a PLC control system to execute regulation and control actions, and acquiring real-time operation parameters of the rectifying tower, inputting the real-time operation parameters into the sample intelligent model to output a simulation regulation and control parameter set, further judging regulation and control feedback, and triggering updating of the sample intelligent model when the regulation and control feedback is in a deviation state. According to the method, through double screening of historical regulation and control parameters and simulation pre-judgment before regulation and control, the accuracy of the regulation and control parameters is improved, and the stability, safety and robustness of the system are enhanced through closed-loop self-learning and fault-tolerant mechanisms.

This invention discloses a quantitative method for phospholipids, comprising: a sample preparation step, wherein a sample containing protein and phospholipids is mixed with Schiff reagent to obtain a sample for testing; a control sample preparation step, wherein the sample is mixed with a solvent of Schiff reagent to obtain a control sample; a measurement step, wherein the degree of a first optical property of the sample for testing and the degree of an optical property of the control sample are measured after the reaction of the aldehyde formed by the vinyl ether bond of phospholipids with Schiff reagent; and a determination step, wherein a correction degree obtained by correcting the degree of the first optical property using the degree of the optical property of the control sample is compared with the degree of a second optical property of the standard sample to determine the concentration of phospholipids in the sample, wherein the standard sample contains Schiff reagent and phospholipids of known concentration, and the degree of the second optical property is the degree of reaction of the Schiff reagent with the aldehyde formed by the vinyl ether bond of phospholipids.

The invention discloses a method for determining related substances in a loxoprofen sodium patch in the technical field of drug detection, and aims to solve the technical blank existing in determination of related substances in a loxoprofen sodium patch in the prior art. The method comprises the following steps: respectively weighing a loxoprofen sodium reference substance and an impurity reference substance, and adding a solvent for dilution to prepare a reference substance solution; taking a loxoprofen sodium patch, stripping a protective layer, adding tetrahydrofuran and methanol, shaking to separate the paste from a backing layer, adding water, shaking again, adding a solvent for dilution, uniformly mixing, centrifuging, and taking supernate to obtain a test solution; injecting the prepared reference substance solution and the test solution into a high performance liquid chromatograph for determination to obtain peak areas of the reference substance solution and the test solution; and calculating the content of the related substances in the loxoprofen sodium patch by adopting an HPLC correction factor method according to the peak areas of the reference solution and the test solution. The method is suitable for measuring the content of the related substances in the loxoprofen sodium patch.

The invention discloses a rapid detection method for talcum powder in food, and relates to the technical field of element analysis and detection. The detection method comprises the following steps: uniformly mixing a to-be-detected sample with nitric acid, perchloric acid and sulfuric acid, heating and digesting, supplementing nitric acid and continuing digesting, and increasing the heating and digesting temperature until the to-be-detected sample is completely digesting to obtain a digesting solution; adding the digestion solution into a colorimetric tube, adding water to a scale, and uniformly shaking to obtain a to-be-detected sample colorimetric solution; mixing another to-be-detected sample with the same mass with talcum powder to obtain a control sample, and treating according to the same method to obtain a control sample colorimetric solution; performing oscillation treatment on the to-be-detected sample colorimetric solution and the control sample colorimetric solution, and performing turbidity comparison under a black background through turbidimetry to obtain a detection result. The method is quick and simple to operate, high in sensitivity, high in accuracy, safe, efficient and energy-saving, the detection period is shortened by half compared with that of a national standard method, and the talcum powder in batch food can be detected at the same time.

The invention provides a self-starting control chart sequence rebound parameter updating method based on warning limit triggering, and relates to the field of statistical process control. Different from a traditional product quality characteristic distribution model based on normal distribution, the method mainly takes product quality monitoring in a high-quality manufacturing process as a target, modeling is performed on an event occurrence interval variable Xt in the process by using gamma distribution, a pivot vector # imgabs0 # is constructed based on the modeling, and mapping from the Xt to self-starting event occurrence interval data St is completed. Further constructing a monitoring statistic Qt of the self-starting control chart by using an exponential weighted moving average method, dynamically switching a parameter updating process based on a time sequence and a sequence bounce according to position information of the Qt in a safety domain, a warning domain and an out-of-control domain of the control chart, delaying the progress that an out-of-control sample flows into parameter estimation, and calculating the out-of-control sample according to the parameter updating process. Data pollution caused by out-of-control samples to parameter estimation is reduced, and the monitoring efficiency of the self-starting control chart is improved.

The application discloses an infrared small target detection network training method and device, equipment and a storage medium, and relates to the technical field of target detection. The method comprises the following steps: based on the number of positive samples and a preset proportion coefficient, difficult negative sample sets and easy negative sample sets are selected from an initial negative sample set; the preset proportion coefficient is a coefficient that is preset to improve the balance degree of the number of positive and negative samples; a weight matrix for controlling samples in a back propagation process is generated according to the difficult negative sample sets and the easy negative sample sets; a difficult point mining loss function is determined based on the weight matrix, and the infrared small target detection network is point supervised and trained by using the difficult point mining loss function. Through the above scheme, the difficult negative sample sets and the easy negative sample sets are determined based on the number of positive samples and the preset proportion coefficient, the unbalanced situation of the number of positive and negative samples in the target detection network training process is prevented, the balance degree of the number of positive and negative samples is improved, and therefore the training effect of the target detection network is improved.

Disclosed are methods of predicting a. subject's responsiveness to an immunotherapy comprising detecting the presence and / or amount of a miR155 gene signature in the subject or a sample from the subject, wherein the miR155 gene signature comprises one or more of miR155, CD3E, CD3G, CD8A, CD8B, CXCR6, FXYD5, GZMB, ID2, IFNgamma, LAGS, NKG7, PDCD1, S100A4, and TIGIT; and comparing the presence and / or amount of the miR155 gene signature to a control sample or threshold, wherein the presence and / or an altered amount of the miR155 gene signature relative to the presence or amount in the control sample or threshold indicates the subject will be responsive or is responding to the immunotherapy.

The invention discloses a fingerprint spectrum of Huanglong cough-relieving granules and a construction method, and relates to the technical field of pharmaceutical analysis. The construction method comprises the following steps: (1) preparation of a test solution: taking Huanglong cough-relieving granules, and adding an extraction solvent for extraction; (2) preparing a reference substance solution; (3) preparing a reference medicinal material solution; (4) chromatographic conditions: octadecylsilane chemically bonded silica is used as a filler; the mobile phase A is acetonitrile, the mobile phase B is 0.1-0.2% phosphoric acid, the flow rate is 0.38-0.42 ml / min, the column temperature is 29-31 DEG C, the detection wavelength is 210 nm in 0-17 min, and the detection wavelength is 270 nm in 17-48 min; and (5) absorbing the solution, and adding the solution into a liquid chromatograph for detection. The fingerprint spectrum constructed by the method can systematically reflect the material basis, is relatively good in precision, repeatability and stability, and can be used for quality control of the Huanglong cough-relieving granules.

The invention relates to the technical field of chemical analysis, and particularly discloses a method and a system for analyzing and calculating the aggregation condition of an analyte in a chemical composition, and the analysis method comprises the following steps: obtaining a chemical image of the analyte in a control sample and a chemical image of the analyte in at least one test sample, carrying out Gaussian blur on each chemical image, and calculating the aggregation condition of the analyte in the chemical composition; carrying out brightness calibration on the obtained Gaussian blurred image; converting the Gaussian blurred image after brightness calibration into a binary image; converting the binary image into a one-dimensional array; counting continuous lengths when predetermined pixels appear in the one-dimensional array and frequencies corresponding to the continuous lengths, and generating a histogram of the frequencies and the continuous lengths; fitting the histogram by using an exponential Gaussian distribution function to obtain an exponential attenuation parameter of the fitted exponential Gaussian distribution function; and calculating the aggregation index of the test sample according to the index attenuation parameters corresponding to the test sample and the control sample. According to the technical scheme, the aggregation condition of the analyte can be quantitatively determined.

The invention relates to a detection method, in particular to a method for detecting aldehyde impurities in polysorbate 20. According to the method, interference caused by the purity of 2, 4-dinitrophenylhydrazine is avoided, derivatization of a reference substance is performed by adopting a method synchronously operating with a test sample, eight aldehyde ketone substances can be detected at the same time, and compared with literature methods, the method is high in specificity, good in precision, high in accuracy, simple and easy to implement and high in operability.

The invention discloses a Jiangtangning granule fingerprint spectrum and a multi-component quantitative quality control method, and belongs to the technical field of pharmaceutical analysis. According to the method, high performance liquid chromatography is adopted, and fingerprint spectrum determination and quantitative analysis of 10 components are synchronously realized under the same chromatographic condition. The method comprises the following steps: preparing a test solution and a reference solution, carrying out gradient elution with an acetonitrile-phosphoric acid aqueous solution, and detecting under optimized chromatographic conditions. The fingerprint spectrum determines common peaks based on multiple batches of samples, and the similarity is greater than or equal to 0.9; 10 components such as calycosin-7-glucoside are measured at the same time, the separation degree is larger than or equal to 1.5, and the linear relation is good. The method solves the problems of few control indexes and low efficiency in the prior art, can comprehensively characterize the product quality, improve the detection efficiency and reduce the cost, and is suitable for quality control of the Huangtangning granules.

This invention discloses a simulated oxidation detection device and method for lead-acid battery casings, including a heating furnace, a beaker placed on the furnace, and an adjusting bracket placed on the side of the furnace. One end of the adjusting bracket extends above the beaker, and a temperature measuring element and a sample are connected to the adjusting bracket, with the temperature measuring element and the sample immersed in cooling water. During detection, the heating furnace is turned on, and cooling water from a workshop water bath is placed in the beaker, with the internal water temperature heated to a stable 40℃±1℃. Two prepared samples are taken: sample A is wrapped with a PE protective film, and sample B is without a film. They are connected to the bracket, with one end immersed in water, and continuously bathed for 24 hours. Finally, the two samples are compared with a control sample. Compared with existing technologies, this invention has a scientifically sound structure, is simple and convenient to operate, and can screen out batches of ABS granules with poor acid resistance and heat resistance at the material stage, preventing them from flowing into subsequent injection molding and battery assembly processes, thus eliminating the phenomenon of discoloration and mottling of the casing from the source.

This application relates to the field of biomedical technology, and in particular to a method for evaluating the effectiveness of autism intervention and its application. The method includes the following steps: collecting a test sample and a control sample; the test sample is a urine sample from an autism patient, and the control sample is a urine sample from a healthy volunteer; processing the test sample and the control sample separately to obtain NMR samples; testing the NMR samples using an NMR spectrometer to obtain NMR spectra; performing multivariate statistical analysis on the NMR spectra to construct a urinary metabolite model; and using the urinary metabolite model to screen and evaluate autism patients. This application provides a method for evaluating the effectiveness of autism intervention and its application, constructing a urinary metabolite model through NMR metabolomics technology, providing practical evidence for establishing methods for early screening and treatment process evaluation of autism.

The utility model discloses a kind of sample falling devices, including supporting mechanism, sample falling mechanism, push sample mechanism and sample inlet mechanism, sample falling mechanism includes the baffle ring disc fixed on supporting mechanism, sample falling disc is coaxially arranged with baffle ring disc and rotatably installed on baffle ring disc, and drive assembly for controlling sample falling disc rotation along circumference is installed on supporting mechanism, the end face of sample falling disc is spaced apart and is equipped with several toothed plates along circumference, and the baffle ring disc between adjacent two toothed plates forms the sample falling groove for accommodating sample ball;Push sample mechanism includes sample receiving assembly for receiving sample ball and being arranged on the outer circumferential side of baffle ring disc, and push sample assembly is installed on baffle ring disc and is used to push sample ball in sample falling groove along the radial direction of baffle ring disc and enter sample receiving assembly;Sample inlet mechanism is installed on baffle ring disc and is used to correspond to be arranged above sample falling groove to transmit sample ball into sample falling groove.The device can control the drop of sample ball, sample falling disc can be positioned and sample is sent, and efficiency is high, and sample ball will not be damaged by friction.

The application discloses a method for screening a quality marker of Radix Paeoniae Rubra based on an anticoagulation spectrum-effect relationship, mainly comprising the following steps: preparing control samples and Radix Paeoniae Rubra sample materials, determining a preparation method of the Radix Paeoniae Rubra sample and optimal chromatographic conditions for separating the Radix Paeoniae Rubra sample, and constructing a fingerprint of the Radix Paeoniae Rubra sample; using a blood coagulation instrument to determine an in-vitro anticoagulation activity of Radix Paeoniae Rubra extracts; analyzing experimental data to determine the quality marker of Radix Paeoniae Rubra based on the anticoagulation spectrum-effect relationship. The efficacy and common peaks of the fingerprint are analyzed by a grey correlation degree analysis method, and the quality marker of Radix Paeoniae Rubra for anticoagulation is screened. The method overcomes the fact that the prior art does not have a related technology for determining the quality marker of Radix Paeoniae Rubra for anticoagulation by the grey correlation degree analysis method.

The invention discloses an irradiation hardening coefficient generation method and an irradiation hardening evaluation method, and relates to the technical field of irradiation hardening. The coefficient generation method comprises the following steps: performing ion irradiation on a test sample, and simulating according to ion irradiation parameters to obtain an irradiation damage depth distribution map; performing nanoindentation test on the test sample subjected to ion irradiation and a control sample of the test sample to obtain a first load displacement curve of the test sample and a second load displacement curve of the control sample; obtaining first and second simulated load displacement curves according to the first and second load displacement curves; obtaining a first elastic-plastic transition point and a second elastic-plastic transition point according to the first load displacement curve, the first simulated load displacement curve, the second load displacement curve and the second simulated load displacement curve; and obtaining the irradiation hardening coefficient according to the first elastic-plastic transition point, the second elastic-plastic transition point and the irradiation damage depth distribution map. Therefore, the radiation hardening can be quantitatively evaluated.

The invention discloses a method for detecting the content of formaldehyde in polyethylene glycol 400, and relates to the technical field of detection. The invention relates to a method for detecting the content of formaldehyde in polyethylene glycol 400, which comprises the following steps: S1, precisely weighing 4-(3-methylphenyl)-2H-pyridine [4, 3, e]-1, 2, 4-thiodiazine-1, 1-dioxo, and adding methanol to dissolve the 4-(3-methylphenyl)-2H-pyridine [4, 3, e]-1, 2, 4-thiodiazine-1, 1-dioxo to prepare a reference solution; s2, precisely weighing polyethylene glycol 400 and reactants to prepare a test solution; and S3, respectively detecting the test solution and the reference solution by adopting a high performance liquid chromatography, and calculating the content of formaldehyde in polyethylene glycol 400, wherein the chromatographic conditions of the high performance liquid chromatography are as follows: gradient elution is adopted; a chromatographic column is characterized in that octadecylsilane chemically bonded silica is used as a stationary phase; the mobile phase comprises a first mobile phase and a second mobile phase, the first mobile phase is a buffer solution, and the second mobile phase is an organic solvent. The method provided by the invention can accurately measure the content of formaldehyde in polyethylene glycol 400.

The present disclosure provides a MNP marker primer combination for macadamia variety identification, a kit and its application. The primer combination comprises: 1st-446th primer pairs, forward primer of 1st primer pair, reverse primer of 1st primer pair to forward primer of 446th primer pair and reverse primer of 446th primer pair, which are sequentially shown in SEQ ID NO: 1-SEQ ID NO: 892 in the sequence listing. Using the MNP marker primer combination, DNA fingerprint data of the sample to be tested can be obtained by multiplex PCR amplification and high-throughput sequencing, and then compared with the control sample to obtain the genetic similarity coefficient, and finally obtain the variety identification conclusion of the sample to be tested and the control sample, and the accuracy and digitization degree are both high. A sequence analysis software can be used to compare hundreds of samples to be tested at one time, and the variety identification conclusion can be quickly obtained, which can significantly improve the accuracy and efficiency of macadamia variety identification.

A DMA replication-associated protein (Rep) for multiple sclerosis (MS) is described, wherein (a) an increased amount of Rep protein or fragments thereof in a sample from a subject compared to an amount in a control sample or an increased amount of anti-Rep protein antibodies to antigen in a sample from a subject compared to an amount in a control sample is associated with a diagnosis of MS, wherein the Rep protein is an MSBI1 Rep or MSBI2 Rep.

This disclosure describes systems, methods, and apparatus for estimation law modules of an adaptive engine. The estimation law modules estimate estimated model parameter tensors for each control sample within a frame. These estimated model parameter tensors are passed to control law modules and used to determine possible control signals as well as being passed to an estimation portion of a nonlinear model of the system, along with the possible control signals, to estimate estimated system outputs corresponding to each of the possible control signals. A selector module can then select a control as one of the possible control signals or a combination of two or more of the possible control signals, based on comparing the corresponding estimated system outputs to a reference signal, and / or measured system outputs.

The invention provides a method for detecting amylase in oyster sauce, which is based on configuration and culture of a buffer solution, a blank control sample and a to-be-detected sample, and finally by observing starch paste states of the to-be-detected sample and the control sample, whether the starch paste state of the to-be-detected sample is thinned is observed, and then whether the to-be-detected sample contains amylase is judged. The method can intuitively and efficiently detect whether the raw material contains amylase, and avoids the technical problems that the color of the oyster sauce finished product interferes with the detection result, the degradation effect of microorganisms on starch causes'false positive 'and the acid degradation effect of acidic raw materials on starch causes'false positive' in the prior art. The oyster sauce finished product is fully prevented from being thinned in the shelf life, and the product quality is ensured.