45results about "Glycosides" patented technology

The invention relates to the field of ginsenoside extraction, in particular to a device and method for removing a plasticizer in a ginsenoside extract, and the device comprises a roll-over stand, a removal cabin and the like. According to the equipment and the method for removing the plasticizer in the ginsenoside extract, the filtering assembly and the adsorption resin blocks are distributed in the removal cabin in the vertical direction, so that fine filtering treatment and adsorption removal treatment of a ginsenoside extract solution are completed in sequence; and in addition, the filter assembly and the plurality of adsorption resin blocks can be subjected to backwashing treatment in a mutually separated state, so that the overall treatment process steps of the backwashing treatment work are reduced on the basis of ensuring that efficient backwashing treatment is synchronously performed on the filter assembly and all the adsorption resin blocks. The defects that multiple treatment devices need to be used in multiple filtering treatment and ion adsorption treatment processes of the ginsenoside extract solution, the plasticizer removal treatment efficiency is affected, and the plasticizer removal treatment period is delayed are overcome.

The invention belongs to the technical field of plant active component extraction and purification, and particularly provides a plant extract purification method based on a deep-eutectic solvent. A plant extract purification method based on a deep-eutectic solvent comprises the following steps: firstly, crushing and grinding a plant to be extracted to obtain fine powder, then heating and uniformly mixing choline chloride, modified amino acid and lewis acid to prepare the deep-eutectic solvent, then mixing the fine powder, the deep-eutectic solvent and water according to a certain solid-to-liquid ratio, and uniformly mixing to obtain the plant extract based on the deep-eutectic solvent. And carrying out ultrasonic extraction at a certain temperature to obtain an extracting solution, and finally purifying. According to the method, the saponin component in the soapberry can be effectively extracted, the saponin yield is high, and the purity is high.

The invention relates to the technical field of eutectic crystals, in particular to an aesculin and mannitol eutectic crystal and a preparation method and application thereof.The XRD spectrum of the eutectic crystal at least has characteristic diffraction peaks at the positions where the angle 2 theta is 13.80 degrees, 17.41 degrees, 18.87 degrees, 19.99 degrees, 20.55 degrees, 21.46 degrees, 25.29 degrees, 27.15 degrees, 27.66 degrees, 28.30 degrees, 33.32 degrees, 34.13 degrees, 35.67 degrees, 36.33 degrees, 36.80 degrees and 44.06 degrees, and error tolerance of + / -0.2 degrees exists; characteristic endothermic peaks exist at 167.5 DEG C and 327 DEG C in a DSC spectrum, and error tolerance of + / -0.2 DEG C exists. The preparation method comprises the following steps: dissolving mannitol and escin in deionized water, heating and stirring, and carrying out high-temperature and high-pressure reaction, standing, crystallizing, filtering and drying to obtain the eutectic crystal which can be applied to cosmetics, health-care products, foods and medicines. The eutectic crystal provided by the invention has the advantages of no irritation, high safety, easy absorption and good stability.

The invention provides a method for extracting QS21 series saponin from a peeled gleditsia sinensis trunk. The QS21 series saponin is prepared by the following steps: performing water extraction on the peeled gleditsia sinensis trunk, adding a stabilizer into an extracting solution, concentrating, separating a concentrated solution by virtue of a multi-stage combined chromatographic column, eluting by virtue of an organic solvent, and collecting and drying eluent. The content of the QS21 saponin extracted by the method disclosed by the invention is 10-35%.

The invention relates to the technical field of natural product separation, and discloses an extraction process of wild jujube seed kernel total saponins, which comprises the following steps: crushing wild jujube seed kernels to obtain wild jujube seed kernel powder; supercritical CO2 extraction is adopted for degreasing; mixing the degreased wild jujube seed kernel powder with an ethanol water solution, and carrying out ultrasonic-assisted extraction at 40-60 DEG C; centrifugally separating the extracting solution; concentrating the supernate under reduced pressure until the volume is 10-20% of the original volume; purifying the concentrated solution through a macroporous adsorption resin column; concentrating the eluent, and dissolving the concentrated eluent in water; and carrying out spray drying on the filtrate to obtain wild jujube kernel total saponin powder. Through a synergistic process of supercritical CO2 degreasing and ultrasonic-assisted extraction and in combination with optimized ethanol concentration and cellulase pretreatment, the extraction time is remarkably shortened, the total extraction time is reduced by 30%-40% compared with that of a traditional method, the yield of total saponins is increased to be larger than or equal to 85%, energy and time needed in the extraction process are reduced, and the utilization rate of raw materials is increased.

The present invention discloses a boric acid-functionalized covalent organic framework microcapsule adsorbent and its preparation method and application. First, TAPB-BTCA-BPTA COFs are synthesized, and Pickering water-in-oil single emulsions are obtained by using TAPB-BTCA-BPTA COFs as stabilizing particles. Using the Pickering water-in-oil emulsion droplets as carriers, a COF shell layer is formed by an autonomous growth method at the emulsion droplet interface for subsequent introduction of azidoboric acid. Finally, the boric acid-functionalized covalent organic framework microcapsule BA-TAPB-BTCA-BPTA MC is prepared by grafting azidoboric acid N3-PBA through the "azide-alkyne" click reaction. It has excellent mechanical strength and stability and is used for the specific adsorption and separation research of active ingredients.

This invention relates to the field of natural product extraction technology, specifically disclosing a self-defoaming extraction and concentration method for total saponins from *Xanthoceras sorbifolium*. The self-defoaming extraction and concentration method provided by this invention utilizes the homology and similarity of saponin components between *Xanthoceras sorbifolium* shell and *Xanthoceras sorbifolium* wood, as well as the differences in non-saponin components. The method involves concentrating the extracts from *Xanthoceras sorbifolium* shell and *Xanthoceras sorbifolium* wood in a specific ratio, or mixing the shells and wood in a specific ratio before extraction. This achieves self-defoaming concentration without any boiling over during the entire concentration process, significantly improving the concentration efficiency of total saponins from *Xanthoceras sorbifolium* and effectively reducing the loss of total saponins during concentration. The total saponin content of the extracted material can reach over 60%. Without adding external defoaming agents and without affecting the quality and efficacy of the saponins, the concentration time is significantly reduced, showing great promise for industrialization.

Application of total monoterpene glycosides of Paeonia veitchii Lynch and analysis method for active ingredients in total monoterpene glycosides of Paeonia veitchii Lynch. For the first time, the UPLC-MS / MS technology is adopted in the present invention to establish the fingerprint of total monoterpene glycosides of Paeonia veitchii Lynch from 11 batches of different producing areas, laying a foundation for revealing the effective ingredient group of total monoterpene glycosides of Paeonia veitchii Lynch. Five compounds with muscle-building effects mainly composed of total monoterpene glycosides of Paeonia veitchii Lynch are determined in the present invention, including 4 monoterpene glycoside compounds, and one belongs to the isomer of benzoylpaeoniflorin. Application of total monoterpene glycosides of Paeonia veitchii Lynch in preparing animal feed or medicine for increasing muscle. The present invention establishes a fingerprint-pharmacodynamic correlation model of total monoterpene glycosides of Paeonia veitchii Lynch. While establishing the quality evaluation standard for muscle-building effects based on the fingerprint of the effective part of total monoterpene glycosides of Paeonia veitchii Lynch, it also provides a new idea for screening active ingredients of other pharmacological effects of Paeonia veitchii Lynch. The present invention lays a theoretical foundation for the development of muscle-building foods for fitness people and provides new resources for the development of animal feed additives in animal husbandry.

This invention relates to the field of natural product separation and recombination, and particularly to a recombinant saponin from *Gleditsia sinensis* and its preparation method. The method includes: 1) crude saponin extraction: water extraction of *Gleditsia sinensis* pericarp to obtain a crude saponin solution; 2) impurity removal: loading the crude saponin aqueous solution into macroporous adsorption resin AB-8 and eluting to remove impurities; 3) saponin component separation: eluting sequentially with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-80% ethanol solution to obtain S30, S45, S60, and S75 saponin components, respectively; 4) saponin hydrolysis: hydrolyzing the S45 saponin component to obtain hydrolyzed saponin; 5) saponin component recombination: recombining the saponin components obtained in steps 3) and 4) with the hydrolyzed saponin to obtain recombinant saponin from *Gleditsia sinensis*. This invention enables saponin purification and improves the surface activity of *Gleditsia sinensis*.

Provided are aqueous lignin solutions containing lignin solvated in Good's Buffers. The aqueous lignin solutions can be used in a variety of applications including biomedical research and in manufacturing a variety of lignin containing products including food grade and biomedical products. The aqueous lignin solutions can also be used in the treatment of microbial infection, as an adjuvant in antimicrobial therapeutics or therapy, and in forming antimicrobial products. Methods of making and using aqueous lignin solutions are also described.

The invention relates to the field of natural medicinal chemistry, and particularly discloses a preparation method of a high-purity saikosaponin extract. The method comprises the following steps: by taking a radix bupleuri medicinal material as a raw material, carrying out raw material pretreatment, ultrasonic-assisted gradient solvent extraction, water precipitation-decoloration combined preliminary purification, specific macroporous adsorption resin dynamic purification, precise drying and the like, and optimizing the extraction solvent concentration (70-85% ethanol), ultrasonic parameters (300-600W, 40-80min) and resin elution gradient (water-> 30% ethanol-> 70-85% ethanol), so as to obtain the radix bupleuri extract. The extraction efficiency and the purity are obviously improved. The total saponin content (in terms of saikosaponin a) in the obtained extract is greater than or equal to 60%, and the total content of saikosaponin a and saikosaponin d is greater than or equal to 40% and can reach 85% or above at most. The method is simple and convenient in process and low in energy consumption, the solvent can be recycled, and the problems of insufficient purity, poor target component selectivity and high industrialization difficulty of a traditional method are solved.

A protein-binding product comprising one or more porous polymer beads, wherein at least one ligand is bound to the surfaces of said polymer beads via a spacer (R), and wherein said at least one ligand is Galβ1-3Hex NAcβ-O-, Galβ1-4Glc NAcβ-O- and / or a derivative thereof, is disclosed as well as a device containing said protein-binding product and a method for reduction of the level of at least one galactose-binding protein and optionally at least one other protein in human blood plasma.

The invention relates to the technical field of plant active ingredient extraction, in particular to a green soapberry active ingredient extraction method. Aiming at the problem that the extraction efficiency of active ingredients of soapberry needs to be improved, the invention provides the following technical scheme: the method comprises the following specific steps: S1, low-temperature composite pretreatment: shelling soapberry fruits, crushing, performing low-temperature freezing treatment, adding a citric acid solution, and stirring to adjust the pH value; s2, double-field synergistic extraction: transferring the pretreated mixed solution into a closed extraction tank, and performing ultrasonic and microwave simultaneous action to obtain an extracting solution; s3, gradient purification and concentration: sequentially performing filtration, adsorption impurity removal and nanofiltration membrane concentration treatment on the extracting solution to obtain a soapberry active ingredient concentrated solution. According to the method, the extraction efficiency of the sapindoside is improved through ultrasonic and microwave double-field synergy, the low-temperature keep-alive rate is high, greenness and no solvent residue are achieved, the process is simple and cost-saving, and the method has the beneficial technical effects of high efficiency, activity protection, environmental protection and easiness in operation.

An aqueous composition comprising: sulfuric acid; a compound comprising an amine moiety; a compound comprising a sulfonic acid moiety; and a peroxide. Said composition being capable of delignifying biomass.

The invention relates to the technical field of health food, and discloses a targeted purification method of PPD type ginsenoside, which can effectively improve the content of PPD type ginsenoside in a ginseng extract by applying HP-20 type macroporous adsorption resin and adopting a specific elution solvent and elution conditions, and has application value.

New use of combination of extraction of Noni and stevia reveals improvement effects on heart failure in animals.

Provided herein are mogrosides containing non-glucose glycosides. Also provided are compositions, including consumables, comprising the mogrosides described herein.

The invention relates to the technical field of extraction of active ingredients of natural plants, in particular to a method for extracting active ingredients of Chinese honeylocust fruits. Aiming at the problems of low extraction efficiency, high energy consumption and the like of the current Chinese honeylocust fruit active ingredient extraction technology, the invention provides the following technical scheme: step 1, raw material pretreatment: purifying, drying and crushing a Chinese honeylocust fruit raw material to obtain Chinese honeylocust fruit powder; step 2, active component extraction: mixing the saponin powder with an extraction medium, adding an enzyme preparation, performing microwave-assisted extraction, and performing solid-liquid separation to obtain supernate containing active components and filter residues; step 3, active component purification: adsorbing, eluting and concentrating the supernate to obtain a saponin active component concentrated solution; and 4, residue treatment: carrying out chemical treatment on the solid-phase residue to obtain a cellulose product. Through the synergistic effect of low-temperature microwaves and composite enzymolysis, oxidative degradation of thermosensitive components is avoided through low-temperature temperature control, the cellulose product is prepared from residues through treatment, and zero-waste utilization of saponin is achieved.

The invention belongs to the technical field of total saponins, and particularly relates to a method for improving the extraction effectiveness of total saponins of ginseng. The preparation method comprises the following steps: pretreating ginseng to obtain a pretreated mixture, carrying out ultrasonic extraction to obtain an extracting solution, stirring and mixing the extracting solution and a composite material, introducing gas in the stirring process, centrifuging after stirring, recycling the composite material, mixing the recycled composite material with a solvent, heating and stirring, centrifuging after stirring, collecting a desorption solution, concentrating and drying to obtain the ginseng extract. Wherein the composite material is obtained by compounding nano-particles and nano-fibers. The extraction effectiveness of the ginseng total saponins can be improved, and the extraction content is increased, so that the extraction efficiency is improved.

The application belongs to the technical field of plant active component extraction and purification, and specifically provides a plant extract purification method based on a deep eutectic solvent. The plant extract purification method based on the deep eutectic solvent comprises the following steps: first, crushing and grinding a plant to be extracted to obtain fine powder; then, heating and uniformly mixing choline chloride, modified amino acid and Lewis acid to prepare a deep eutectic solvent; then, mixing the fine powder, the deep eutectic solvent and water according to a certain solid-liquid ratio, and performing ultrasonic extraction at a certain temperature to obtain an extraction liquid; and finally, performing purification. The above method can effectively extract saponin components in soapberry, and the saponin has high yield and high purity.

This invention relates to the field of natural saponin separation and recombination, and particularly to a complex of saponin from *Gleditsia sinensis* and saponin from *Camellia oleifera* and its preparation method. The method includes: 1) extracting saponin from *Gleditsia sinensis* pericarp and *Camellia oleifera* cake, respectively, to obtain crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 2) using macroporous adsorption resin AB-8 as a resin separation column to elute and remove impurities from the crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 3) eluting the obtained saponin solutions from *Gleditsia sinensis* and *Camellia oleifera* with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-85% ethanol solution, respectively, to obtain saponin components from *Gleditsia sinensis* and saponin components from *Camellia oleifera*; 4) recombining the saponin components from *Gleditsia sinensis* and *Camellia oleifera* to obtain the complex of saponin from *Gleditsia sinensis* and *Camellia oleifera*. This invention can improve the purity and surface activity of natural saponins, overcoming the disadvantage of the high critical micelle concentration of *Camellia oleifera*.

The invention discloses a method for preparing high-purity sapindoside powder, and belongs to the technical field of forest chemical industry. The method provided by the invention comprises the following steps: extracting saponin in soapberry pericarp by using a leaching solution containing a mixed enzyme, Mg < 2 + > and a phosphate buffer system, performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 6000-10000 Daltons, concentrating by using an ultrafiltration membrane with the molecular weight cutoff of 200-600 Daltons, and drying, the soapberry peel solid powder with the saponin purity reaching 90% or above can be obtained.

The invention relates to the field of extraction of effective parts of traditional Chinese medicine compounds, in particular to a method for flash extraction of total saponins of pistachio powder by using a deep-eutectic solvent, and can effectively solve the problems that significant polarity difference exists between tenuigenin and ginsenoside; the contents of the two types of saponins are difficult to efficiently extract and balance at the same time; and the existing extraction method has the problems of time consumption, pollution and waste. The method comprises the following steps: mixing the medicinal materials in the Kaixin powder, adding into a deep eutectic solvent aqueous solution, carrying out flash extraction, filtering to obtain a Kaixin powder extracting solution, carrying out separation and purification by using macroporous adsorption resin, respectively eluting by using water, 20% ethanol and 60% ethanol, collecting the eluent of the 60% ethanol part, concentrating, and drying to a powder state, wherein the 60% ethanol part is a saponin component. According to the method, the extraction efficiency and total content of the target saponin are remarkably improved, the characteristics of multiple components and multiple targets of traditional Chinese medicines are ensured, meanwhile, energy consumption is reduced, time is shortened, medicinal material waste is reduced, and the green and environment-friendly characteristics of the process are improved.

The invention provides a method for extracting total saponins in Jinping ginseng, which comprises the following steps of: 1, constructing a Jinping ginseng extraction process prediction model by taking ethanol concentration, extraction times and ultrasonic temperature as input of a model and taking total saponin extraction rate as output; 2, selecting an extraction process corresponding to the maximum total saponin extraction rate by using the constructed Jinping ginseng extraction process prediction model; and 3, extracting the total saponins of the Jinping ginseng by using an extraction process corresponding to the maximum total saponin extraction rate. According to the method provided by the invention, the extraction rate of the total saponins in the Jinping ginseng can be effectively improved.

The invention provides a method and process for extracting total asiaticoside from centella asiatica, and relates to the technical field of organic chemical agent manufacturing, and the extraction method comprises the following steps: pretreating and crushing raw materials; performing water reflux extraction; adsorbing and purifying by using macroporous resin; carrying out alcohol precipitation impurity removal and concentration, controlling the evaporation temperature and the vacuum degree in the concentration process to adjust the feeding flow rate, so that the liquid medicine uniformly forms a film, and concentrating until the specific gravity of the liquid medicine is 1.2 to obtain thick paste; and closing the heating system after concentration is finished. According to the invention, the heat loss rate of asiaticoside can be reduced, the output is increased, and the circulation rate is increased through a condensation recovery system, so that the consumable cost and the equipment cost are effectively reduced, efficient and mild material treatment is realized through a falling film evaporation technology, and by utilizing the synergistic effect of film flow and vacuum low temperature, the product quality is improved. And the solvent is quickly removed within an extremely short heating time, so that the heat-sensitive asiaticoside is protected from being degraded and discolored to the greatest extent.

Provided herein are mogrosides, including non-glucose glycosides. Also provided are compositions, including consumables, that contain the novel mogrosides described herein.

The application provides a method for purifying polycyclic aromatic hydrocarbons (PAHs) in gynostemma extract, and the presence of PAHs in gynostemma extract is found for the first time, and the PAHs in the gynostemma extract are removed in a targeted manner, wherein the purification process is to remove the PAHs in the extract by using activated carbon under specific conditions, and the gynostemma extract is dissolved more fully by selecting ethanol solution as a solvent and controlling the dissolving process, so as to provide a basis for subsequent purification, and the activated carbon purification process is optimized, the amount of activated carbon and the purification temperature are controlled, and the content of PAHs in the gynostemma extract is greatly reduced by adjusting the overall solvent process and the purification process, and the loss of the gynostemma extract is reduced. The whole process is simple, cost-effective, environmentally friendly, can obviously improve the quality of the extract, and meets the strict quality standards.

The invention relates to the technical field of separation and purification of natural functional components, in particular to a resin cross-linking agent, acylthiourea-containing rosin-based adsorption resin and a preparation method and application of the acylthiourea-containing rosin-based adsorption resin. The preparation method of the cross-linking agent comprises the following steps: reacting maleated rosin with SOCl2 to obtain an intermediate product A, reacting the intermediate product A with potassium thiocyanate, reacting the intermediate product B with aniline-o-methylaniline to obtain an intermediate product C, and reacting the intermediate product C with ethyl acetate and hydroxyethyl acrylate to obtain the cross-linking agent NMDMR. The adsorption resin is synthesized by taking NMDMR as a cross-linking agent and diethylbenzene (DVB) and methacrylic acid (MAA) as monomers through a suspension polymerization method. The PNS adsorption capacity of the obtained rosin-based resin is 221.11 mg / g, and the rosin-based resin has good adsorption separation and purification capacity on PNS, and is a renewable and efficient rosin-based resin adsorbent.

The invention belongs to the technical field of separation and extraction, and particularly relates to a method for separating flavone and saponin from wild jujube leaves as well as a preparation method and application of the flavone and the saponin. The method provided by the invention comprises the following steps: 1) mixing wild jujube leaf powder with water, carrying out first ultrasonic treatment, then carrying out first heat reflux extraction, and separating to obtain an extracting solution A and solid residues; 2) mixing the solid residues with an alcohol-water solution, carrying out second ultrasonic treatment, then carrying out second heat reflux extraction, and separating to obtain an extracting solution B; and (3) mixing the extracting solution A and the extracting solution B, concentrating to remove ethanol to form a concentrated solution, and then carrying out gradient elution on the concentrated solution by using a macroporous resin column formed by compounding D101 macroporous resin and HPD-600 macroporous resin to obtain the flavone-rich extract and the saponin-rich extract. According to the method, the flavone-rich extract and the saponin-rich extract can be simultaneously and efficiently extracted and separated from the wild jujube leaves.

The application discloses a soapwort flower integrated production process and a preparation method of a washing base material thereof, and the method comprises the following steps: in a closed space, mild biological enzymolysis is sequentially performed on pretreated soapwort flower raw materials to release active ingredients, then multi-stage membrane separation technology is used to perform online purification and concentration on the enzymolysis liquid, high-purity extracts are obtained, finally, the extracts are dynamically mixed and homogenized with functional material flow including other plant saponins, natural washing aids, viscosity regulators and preservatives, and the finished product base material is directly output, the method integrates enzymolysis, membrane separation and online compounding, realizes full-process continuous production and clean production, avoids damage of heat-sensitive components and exogenous pollution, and the obtained base material utilizes the synergistic effect of natural components, has excellent washing performance, ecological safety and skin mildness, and provides a new strategy for developing high-efficiency and environmentally-friendly washing products.