25results about "Glycosides" patented technology

The invention belongs to the technical field of plant active component extraction and purification, and particularly provides a plant extract purification method based on a deep-eutectic solvent. A plant extract purification method based on a deep-eutectic solvent comprises the following steps: firstly, crushing and grinding a plant to be extracted to obtain fine powder, then heating and uniformly mixing choline chloride, modified amino acid and lewis acid to prepare the deep-eutectic solvent, then mixing the fine powder, the deep-eutectic solvent and water according to a certain solid-to-liquid ratio, and uniformly mixing to obtain the plant extract based on the deep-eutectic solvent. And carrying out ultrasonic extraction at a certain temperature to obtain an extracting solution, and finally purifying. According to the method, the saponin component in the soapberry can be effectively extracted, the saponin yield is high, and the purity is high.

The invention provides a method for extracting QS21 series saponin from a peeled gleditsia sinensis trunk. The QS21 series saponin is prepared by the following steps: performing water extraction on the peeled gleditsia sinensis trunk, adding a stabilizer into an extracting solution, concentrating, separating a concentrated solution by virtue of a multi-stage combined chromatographic column, eluting by virtue of an organic solvent, and collecting and drying eluent. The content of the QS21 saponin extracted by the method disclosed by the invention is 10-35%.

This invention relates to the field of natural product separation and recombination, and particularly to a recombinant saponin from *Gleditsia sinensis* and its preparation method. The method includes: 1) crude saponin extraction: water extraction of *Gleditsia sinensis* pericarp to obtain a crude saponin solution; 2) impurity removal: loading the crude saponin aqueous solution into macroporous adsorption resin AB-8 and eluting to remove impurities; 3) saponin component separation: eluting sequentially with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-80% ethanol solution to obtain S30, S45, S60, and S75 saponin components, respectively; 4) saponin hydrolysis: hydrolyzing the S45 saponin component to obtain hydrolyzed saponin; 5) saponin component recombination: recombining the saponin components obtained in steps 3) and 4) with the hydrolyzed saponin to obtain recombinant saponin from *Gleditsia sinensis*. This invention enables saponin purification and improves the surface activity of *Gleditsia sinensis*.

Provided are aqueous lignin solutions containing lignin solvated in Good's Buffers. The aqueous lignin solutions can be used in a variety of applications including biomedical research and in manufacturing a variety of lignin containing products including food grade and biomedical products. The aqueous lignin solutions can also be used in the treatment of microbial infection, as an adjuvant in antimicrobial therapeutics or therapy, and in forming antimicrobial products. Methods of making and using aqueous lignin solutions are also described.

The invention relates to the field of natural medicinal chemistry, and particularly discloses a preparation method of a high-purity saikosaponin extract. The method comprises the following steps: by taking a radix bupleuri medicinal material as a raw material, carrying out raw material pretreatment, ultrasonic-assisted gradient solvent extraction, water precipitation-decoloration combined preliminary purification, specific macroporous adsorption resin dynamic purification, precise drying and the like, and optimizing the extraction solvent concentration (70-85% ethanol), ultrasonic parameters (300-600W, 40-80min) and resin elution gradient (water-> 30% ethanol-> 70-85% ethanol), so as to obtain the radix bupleuri extract. The extraction efficiency and the purity are obviously improved. The total saponin content (in terms of saikosaponin a) in the obtained extract is greater than or equal to 60%, and the total content of saikosaponin a and saikosaponin d is greater than or equal to 40% and can reach 85% or above at most. The method is simple and convenient in process and low in energy consumption, the solvent can be recycled, and the problems of insufficient purity, poor target component selectivity and high industrialization difficulty of a traditional method are solved.

A protein-binding product comprising one or more porous polymer beads, wherein at least one ligand is bound to the surfaces of said polymer beads via a spacer (R), and wherein said at least one ligand is Galβ1-3Hex NAcβ-O-, Galβ1-4Glc NAcβ-O- and / or a derivative thereof, is disclosed as well as a device containing said protein-binding product and a method for reduction of the level of at least one galactose-binding protein and optionally at least one other protein in human blood plasma.

The invention relates to the technical field of plant active ingredient extraction, in particular to a green soapberry active ingredient extraction method. Aiming at the problem that the extraction efficiency of active ingredients of soapberry needs to be improved, the invention provides the following technical scheme: the method comprises the following specific steps: S1, low-temperature composite pretreatment: shelling soapberry fruits, crushing, performing low-temperature freezing treatment, adding a citric acid solution, and stirring to adjust the pH value; s2, double-field synergistic extraction: transferring the pretreated mixed solution into a closed extraction tank, and performing ultrasonic and microwave simultaneous action to obtain an extracting solution; s3, gradient purification and concentration: sequentially performing filtration, adsorption impurity removal and nanofiltration membrane concentration treatment on the extracting solution to obtain a soapberry active ingredient concentrated solution. According to the method, the extraction efficiency of the sapindoside is improved through ultrasonic and microwave double-field synergy, the low-temperature keep-alive rate is high, greenness and no solvent residue are achieved, the process is simple and cost-saving, and the method has the beneficial technical effects of high efficiency, activity protection, environmental protection and easiness in operation.

New use of combination of extraction of Noni and stevia reveals improvement effects on heart failure in animals.

Provided herein are mogrosides containing non-glucose glycosides. Also provided are compositions, including consumables, comprising the mogrosides described herein.

The application belongs to the technical field of plant active component extraction and purification, and specifically provides a plant extract purification method based on a deep eutectic solvent. The plant extract purification method based on the deep eutectic solvent comprises the following steps: first, crushing and grinding a plant to be extracted to obtain fine powder; then, heating and uniformly mixing choline chloride, modified amino acid and Lewis acid to prepare a deep eutectic solvent; then, mixing the fine powder, the deep eutectic solvent and water according to a certain solid-liquid ratio, and performing ultrasonic extraction at a certain temperature to obtain an extraction liquid; and finally, performing purification. The above method can effectively extract saponin components in soapberry, and the saponin has high yield and high purity.

This invention relates to the field of natural saponin separation and recombination, and particularly to a complex of saponin from *Gleditsia sinensis* and saponin from *Camellia oleifera* and its preparation method. The method includes: 1) extracting saponin from *Gleditsia sinensis* pericarp and *Camellia oleifera* cake, respectively, to obtain crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 2) using macroporous adsorption resin AB-8 as a resin separation column to elute and remove impurities from the crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 3) eluting the obtained saponin solutions from *Gleditsia sinensis* and *Camellia oleifera* with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-85% ethanol solution, respectively, to obtain saponin components from *Gleditsia sinensis* and saponin components from *Camellia oleifera*; 4) recombining the saponin components from *Gleditsia sinensis* and *Camellia oleifera* to obtain the complex of saponin from *Gleditsia sinensis* and *Camellia oleifera*. This invention can improve the purity and surface activity of natural saponins, overcoming the disadvantage of the high critical micelle concentration of *Camellia oleifera*.

The invention provides a method and process for extracting total asiaticoside from centella asiatica, and relates to the technical field of organic chemical agent manufacturing, and the extraction method comprises the following steps: pretreating and crushing raw materials; performing water reflux extraction; adsorbing and purifying by using macroporous resin; carrying out alcohol precipitation impurity removal and concentration, controlling the evaporation temperature and the vacuum degree in the concentration process to adjust the feeding flow rate, so that the liquid medicine uniformly forms a film, and concentrating until the specific gravity of the liquid medicine is 1.2 to obtain thick paste; and closing the heating system after concentration is finished. According to the invention, the heat loss rate of asiaticoside can be reduced, the output is increased, and the circulation rate is increased through a condensation recovery system, so that the consumable cost and the equipment cost are effectively reduced, efficient and mild material treatment is realized through a falling film evaporation technology, and by utilizing the synergistic effect of film flow and vacuum low temperature, the product quality is improved. And the solvent is quickly removed within an extremely short heating time, so that the heat-sensitive asiaticoside is protected from being degraded and discolored to the greatest extent.

The application provides a method for purifying polycyclic aromatic hydrocarbons (PAHs) in gynostemma extract, and the presence of PAHs in gynostemma extract is found for the first time, and the PAHs in the gynostemma extract are removed in a targeted manner, wherein the purification process is to remove the PAHs in the extract by using activated carbon under specific conditions, and the gynostemma extract is dissolved more fully by selecting ethanol solution as a solvent and controlling the dissolving process, so as to provide a basis for subsequent purification, and the activated carbon purification process is optimized, the amount of activated carbon and the purification temperature are controlled, and the content of PAHs in the gynostemma extract is greatly reduced by adjusting the overall solvent process and the purification process, and the loss of the gynostemma extract is reduced. The whole process is simple, cost-effective, environmentally friendly, can obviously improve the quality of the extract, and meets the strict quality standards.

The invention relates to the technical field of separation and purification of natural functional components, in particular to a resin cross-linking agent, acylthiourea-containing rosin-based adsorption resin and a preparation method and application of the acylthiourea-containing rosin-based adsorption resin. The preparation method of the cross-linking agent comprises the following steps: reacting maleated rosin with SOCl2 to obtain an intermediate product A, reacting the intermediate product A with potassium thiocyanate, reacting the intermediate product B with aniline-o-methylaniline to obtain an intermediate product C, and reacting the intermediate product C with ethyl acetate and hydroxyethyl acrylate to obtain the cross-linking agent NMDMR. The adsorption resin is synthesized by taking NMDMR as a cross-linking agent and diethylbenzene (DVB) and methacrylic acid (MAA) as monomers through a suspension polymerization method. The PNS adsorption capacity of the obtained rosin-based resin is 221.11 mg / g, and the rosin-based resin has good adsorption separation and purification capacity on PNS, and is a renewable and efficient rosin-based resin adsorbent.

The invention belongs to the technical field of separation and extraction, and particularly relates to a method for separating flavone and saponin from wild jujube leaves as well as a preparation method and application of the flavone and the saponin. The method provided by the invention comprises the following steps: 1) mixing wild jujube leaf powder with water, carrying out first ultrasonic treatment, then carrying out first heat reflux extraction, and separating to obtain an extracting solution A and solid residues; 2) mixing the solid residues with an alcohol-water solution, carrying out second ultrasonic treatment, then carrying out second heat reflux extraction, and separating to obtain an extracting solution B; and (3) mixing the extracting solution A and the extracting solution B, concentrating to remove ethanol to form a concentrated solution, and then carrying out gradient elution on the concentrated solution by using a macroporous resin column formed by compounding D101 macroporous resin and HPD-600 macroporous resin to obtain the flavone-rich extract and the saponin-rich extract. According to the method, the flavone-rich extract and the saponin-rich extract can be simultaneously and efficiently extracted and separated from the wild jujube leaves.

The application discloses a soapwort flower integrated production process and a preparation method of a washing base material thereof, and the method comprises the following steps: in a closed space, mild biological enzymolysis is sequentially performed on pretreated soapwort flower raw materials to release active ingredients, then multi-stage membrane separation technology is used to perform online purification and concentration on the enzymolysis liquid, high-purity extracts are obtained, finally, the extracts are dynamically mixed and homogenized with functional material flow including other plant saponins, natural washing aids, viscosity regulators and preservatives, and the finished product base material is directly output, the method integrates enzymolysis, membrane separation and online compounding, realizes full-process continuous production and clean production, avoids damage of heat-sensitive components and exogenous pollution, and the obtained base material utilizes the synergistic effect of natural components, has excellent washing performance, ecological safety and skin mildness, and provides a new strategy for developing high-efficiency and environmentally-friendly washing products.

This invention provides a method for efficiently extracting rare ginsenosides from Panax notoginseng stems and leaves, comprising the following steps: 1) raw material pretreatment, 2) cell wall disruption sterilization and saponin preconversion, 3) fermentation substrate preparation, 4) constant temperature aerobic fermentation, and 5) post-treatment, to obtain a high-activity rare ginsenoside product. This method solves the industry pain points of low saponin conversion efficiency, poor fermentation system stability, and difficulty in adapting thermal conversion and biological fermentation in existing technologies. It achieves full-process efficiency enhancement of saponin conversion, and increases the raw material utilization rate to over 90%. It also features high efficiency, greenness, low cost, and stable process, which is of great significance for increasing industrial added value and realizing high-value utilization of resources.

This invention discloses a method and its application for simultaneously extracting flavonoids and iridoid glycosides from Eucommia ulmoides male flowers using a deep eutectic solvent. The method includes pretreatment of Eucommia ulmoides male flowers, preparation of the deep eutectic solvent, and extraction of flavonoids and iridoid glycosides from the flowers. The deep eutectic solvent provided by this invention is prepared from betaine and citric acid, and is characterized by being green, natural, safe, non-toxic, simple in preparation, and low in cost. It not only increases the extraction rate of bioactive compounds from Eucommia ulmoides male flowers, but also significantly enhances the antioxidant and anti-aging bioactivity of Eucommia ulmoides male flowers compared to organic solvents, thereby increasing the content of total flavonoids and iridoid glycosides in the extract. Therefore, it can be used as a novel solvent for the preparation of liquid formulations of Eucommia ulmoides male flower extract, enabling liquid addition.

The present application relates to the field of biotechnology, and more particularly to a kind of alfalfa transient expression cellulase and its application in Xinguan fruit leaf active substance extraction, comprising the following steps: (1) cellulase coding gene construction vector, transformation agrobacterium, culture, centrifugation, collection of bacteria, suspended in buffer, dilution;(2) the bacteria solution obtained in step (1) is used to carry out vacuum infiltration on alfalfa leaf, culture, and 1-500 μM melatonin is sprayed on alfalfa leaf, and spraying is carried out once a day;(3) the alfalfa leaf obtained in step (2) is ground, homogenized, centrifuged, and supernatant is extracted to obtain cellulase enzyme solution;Melatonin is applied in the process of expression, and the activity of cellulase obtained is significantly improved, the problems of low activity of plant expressed cellulase and extraction of leaf active substance biological enzyme are solved;And the steps are simple, the cellulase prepared does not contain endotoxin, can be used for Xinguan fruit leaf active substance extraction, and has wide application prospect.

The invention belongs to the field of microbial fermentation, and relates to a preparation method for improving jujuboside in fermentation liquor and the fermentation liquor. The preparation method comprises the following steps: S1, crushing spina date seeds to obtain spina date seed powder; adding water into the spina date seed powder to prepare spina date seed powder slurry; s2, adding a plant cell wall degrading enzyme into the spina date seed powder slurry, and carrying out an enzymolysis reaction to destroy a cell structure until the reaction is complete, so as to obtain an enzymolysis product; and S3, inoculating a microbial agent containing lactobacillus paracasei into the enzymolysis product, and fermenting to obtain fermentation liquor rich in jujuboside. According to the method, the jujuboside in the fermentation liquor can be efficiently converted and enriched through a means of combining enzymolysis treatment and specific microbial fermentation, and meanwhile, the method is simple and suitable for large-scale production. The obtained fermentation liquor is rich in jujuboside and GABA (gamma-aminobutyric acid), and has outstanding application potential and market value.

The method comprises the following steps: adding a non-alcohol polar solvent into a concentrated soapberry bark powder water extract, stirring at low temperature to separate out impurities, and filtering to obtain a soapberry saponin concentrated solution from which the polysaccharide and polyphenol are removed. According to the method, a non-alcohol polar solvent low-temperature precipitation mode is adopted, and polysaccharides and polyphenols in the saponin of the gleditsia sinensis can be effectively removed. Part of polysaccharides and polyphenols are removed from the obtained saponin concentrated solution, the risk of blocking a preparative column can be reduced, solvent replacement is not needed, the influence on the saponin concentration in the process is small, and saponin compounds such as QS-21 and the like can be directly prepared.

The invention belongs to the technical field of lilium glycoside substance extraction, and discloses a method for enriching lilium lancifolium lilium glycoside. The method comprises the following steps: 1) mixing lily bulb powder with water, and adjusting the pH value to 4.4-4.6 to obtain lily slurry; (2) carrying out enzymolysis on the lily pulp at 50 DEG C for 2 hours by adopting pectinase and cellulase, then carrying out enzymolysis at 85 DEG C for 2 hours by adopting amylase, cooling to 60 DEG C, and adding saccharifying enzyme for enzymolysis for 1 hour, so as to obtain enzymatic hydrolysate; and 3) adding acetic bacteria into the enzymatic hydrolysate for fermentation. According to the method disclosed by the invention, the total content of phenolic substances in the lilium lancifolium bulbs is increased by more than 7 times, and bulbus lilii glycoside B, bulbus lilii glycoside E and bulbus lilii glycoside H are extracted, so that remarkable enrichment of the bulbus lilii glycoside substances is realized. Meanwhile, the antioxidant activity of the fermentation product is obviously enhanced. The yield of lilium lancifolium glycoside active substances can be remarkably increased, the biological activity function of the lilium lancifolium glycoside active substances is effectively improved, and the method has the advantages of being stable in process, suitable for industrial production and the like.

The invention discloses a method for preparing saponin from nine-time steamed and nine-time sun-dried rhizoma polygonati, the prepared saponin and application of the saponin. The method comprises the following steps: (1) processing rhizoma polygonati into powder through nine times of steaming and nine times of sun-drying; (2) performing alcohol extraction to obtain a nine-steaming and nine-sun-drying rhizoma polygonati total saponin alcohol extract; (3) performing rotary evaporation and concentration to obtain a nine-steaming and nine-sun-drying polygonatum sibiricum total saponin concentrated solution; (4) removing fat-soluble impurities to obtain nine-steamed nine-sun-dried polygonatum sibiricum total saponin degreasing fluid; (5) extracting to obtain a nine-steaming nine-sun-drying rhizoma polygonati total saponin extracting solution; (6) obtaining a composite chromatographic column; (7) sequentially carrying out linear gradient elution and step gradient elution on the nine-steaming and nine-sun-drying rhizoma polygonati total saponin extraction liquid by adopting a composite chromatographic column to obtain a saponin component P0 with anti-inflammatory activity; and separating an intermediate product obtained by linear gradient elution through an ultrafiltration membrane to obtain a saponin component P2 with whitening activity. According to the method, high-purity anti-inflammatory and whitening active saponin components are sequentially enriched, and the extraction yield and purity of target components are remarkably improved while the operation process is optimized.

The invention discloses a semen lepidii-poria cocos-based cardiac glycoside compound, and relates to the technical field of traditional Chinese medicine pharmacy, the compound comprises the following components: semen lepidii-poria cocos extract powder, a complex enzyme preparation, and an ethanol aqueous solution with a volume fraction of 50%; the compound enzyme preparation is prepared from pectinase, cellulase and beta-glucosidase; according to the method, semen lepidii and poria cocos raw material combination and a compound enzyme two-phase enzymolysis system are combined with ultrasonic-assisted extraction, membrane separation purification and vacuum freeze-drying technologies, efficient extraction and high-purity enrichment of cardiac glycoside compounds are achieved, the raw material utilization rate and product purity are synergistically optimized in all links, meanwhile, energy consumption and pollution are reduced, the process is standard and controllable, and the method is suitable for industrial production. The method provides a reliable path for large-scale preparation of high-activity and high-purity cardiac glycoside, and has a wide application prospect.

Mogrosides containing non-glucose glycosides are provided herein. Compositions, including consumables comprising the novel mogrosides described herein, are also provided.