56 results about "Saporin" patented technology

In one aspect, the disclosure relates to immunogenic compositions comprising a semisynthetic saponin adjuvant (VSA) including, but not limited to, VSA-1 and VSA-2, derived from a Momordica saponin and at least one antigen. In a further aspect, the immunogenic compositions can include other known adjuvants, emulsifiers, and / or surface-active agents and can be formulated as liposomes, micelles, emulsions, and the like. Also disclosed are methods of eliciting effective immune responses to viral, bacterial, protozoal, and neoplastic diseases using the disclosed immunogenic compositions.

The invention discloses a method for screening and extracting DES of tea saponin based on molecular simulation and machine learning, and relates to the technical field of tea saponin extraction.The method comprises the steps that tea saponin, cellulose, a hydrogen bond donor, a hydrogen bond receptor and a DES molecular model are constructed and optimized, and a molecular polarity index is obtained; the method comprises the following steps: generating a topology, obtaining a trajectory and energy through MD simulation, and calculating E (TeaS-DES), E (TeaS-Cell) and SI; and measuring the extraction rate, combining the molecular polarity index, SI, process parameters and the like to construct a data set to train a machine learning model, predicting the extraction rate, and locking the optimal DES and process. According to the method, DES screening can be directly guided through simulated energy and structure descriptors, the number of experiments is reduced, and the research and development period is shortened.

The invention discloses application of a saponin enzymolysis product in prevention and treatment of plutella xylostella, and belongs to the technical field of biopesticides. According to the method, gleditsia sinensis saponin is degraded by using biological enzyme, and the obtained saponin degradation product has excellent food refusal, contact toxicity and stomach toxicity activity on plutella xylostella. The raw materials are obtained from natural plants, the preparation method is green and safe, the activity of degradation products is high, use of chemical pesticides can be reduced, degradation can be achieved in the natural environment, and a novel green prevention and control agent and technology can be provided for prevention and control of plutella xylostella.

The invention discloses a multi-scene deodorizing detergent composition and a preparation method thereof, and relates to the technical field of daily chemical industry. The multi-scene deodorizing detergent composition provided by the invention is prepared from the following core components: alkyl glycoside, sodium lauroamphoacetate, tea saponin, hydroxypropyl-beta-cyclodextrin, chitosan, compound enzyme, sodium cinnamate, dragon fruit extract, humulus lupulus extract and the like. Through the synergistic effect of all the components, the composition realizes integration of multiple effects: the irritation is remarkably reduced and the skin barrier is maintained while efficient decontamination is realized; an adsorption-degradation system formed by hydroxypropyl-beta-cyclodextrin, chitosan and compound enzyme can radically remove peculiar smell and efficiently degrade pesticide residues; the chitosan and the pitaya extract synergistically chelate heavy metal ions; and components such as sodium cinnamate have natural whitening and broad-spectrum preservative functions. The technical limitation that a traditional product is single in function is broken through, and a comprehensive and safe washing solution with a positive skin care effect is provided.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The invention discloses application of alkalized tea saponin as a talc inhibitor, and belongs to the technical field of mineral separation. The molecular structural formula of the alkalized tea saponin is shown in the specification, wherein R is a sugar body unit; m is a cation; the alkalized tea saponin has a selective inhibition effect on flotation of talc, and is particularly suitable for flotation separation of metal sulfide ore (such as molybdenite, chalcopyrite, galena and sphalerite) and talc.

The invention relates to the technical field of biochemical wastewater treatment and automatic control, in particular to a comprehensive utilization and treatment system for dioscorea zingiberensis saponin wastewater. The data acquisition module comprises a data acquisition module, a prediction module, a quantification module, a risk hedging module and a control execution module; the system estimates key enzyme activity by adopting a non-linear model by collecting a water inlet fingerprint vector and a reactor state; the core of the method is that a system safety distance is determined according to enzyme activity and flora health degree, and control weights of three targets of collapse risk, effluent compliance and resource recovery are dynamically adjusted according to the system safety distance; when the safe distance changes, the system executes compound control through a multi-objective optimization algorithm; according to the method, prospective early warning of the core biochemical state is realized, and the defect of response lag of a traditional method is overcome.

This invention relates to the field of natural product separation and recombination, and particularly to a recombinant saponin from *Gleditsia sinensis* and its preparation method. The method includes: 1) crude saponin extraction: water extraction of *Gleditsia sinensis* pericarp to obtain a crude saponin solution; 2) impurity removal: loading the crude saponin aqueous solution into macroporous adsorption resin AB-8 and eluting to remove impurities; 3) saponin component separation: eluting sequentially with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-80% ethanol solution to obtain S30, S45, S60, and S75 saponin components, respectively; 4) saponin hydrolysis: hydrolyzing the S45 saponin component to obtain hydrolyzed saponin; 5) saponin component recombination: recombining the saponin components obtained in steps 3) and 4) with the hydrolyzed saponin to obtain recombinant saponin from *Gleditsia sinensis*. This invention enables saponin purification and improves the surface activity of *Gleditsia sinensis*.

We provide novel, specific cytotoxic drug therapies with minimal side effects. [Solution] A composition for a membrane-permeable complex for transport across a lipid membrane is provided, comprising one or more molecular induction system (MGS) peptides and a cytotoxic agent. A method for treating cancer is also provided, comprising administering this composition to a cancer patient.

Provided are variants of saponins that are found in Quillaja saponaria (QS) Molina tree bark that are chemically modified to distinguish them from naturally occurring parent saponins. The modified saponins have increased adjunctive activity compared to the unmodified parent saponins. Defined structures allow for comparisons of the modifying groups with respect to their respective adjunct activity and permit characterized vaccine formulations that have one or more defined saponins.

The invention relates to the technical field of aviation plant protection aids, and discloses an aviation plant protection aid containing natural plant polysaccharides and a preparation method of the aviation plant protection aid. Constructing a dynamic self-repairing system of a laccase catalysis phenol crosslinking component, a peroxidase catalysis polysaccharide coupling component and a cellulose degradation product repairing storage material, and a biodegradation regulation and control system integrating a cellulase-xylanase compound enzyme component, a tea saponin regulation component and a peptone nutrition component; the functions of enhancing leaf surface attachment, dynamic self-repairing and environment-friendly degradation of the auxiliary agent are realized through multiple synergistic effects. The technical problems that a traditional aviation plant protection auxiliary is insufficient in leaf surface adhesive force, damage cannot be automatically repaired, and environment residues are accumulated are solved, stable adhesion of liquid medicine under the severe weather condition, automatic repairing capacity after film damage and complete biodegradation within 30 days after the pesticide effect period is finished are achieved, and the effect is achieved. And the reliability, persistence and environmental friendliness of aviation plant protection are remarkably improved.

This invention relates to the field of natural material emulsification and discloses a natural material emulsification process for processing personal care products. The process includes: injecting a dispersed aqueous phase of tea saponin and soybean lecithin into the oil phase at a gradually decreasing pump flow rate at a temperature ranging from 30°C to 42°C, while simultaneously increasing the stirring speed; applying an ultrasonic pulse after the oil phase injection is complete; lowering the stirring speed to stabilize the interfacial film; and reducing the temperature in two stages. This invention matches the interfacial expansion rate with the molecular adsorption rate to eliminate localized oil phase overload, resulting in a uniform monodisperse state of the droplets. Simultaneously, it avoids the peeling and damage of the interfacial film by continuous strong shear fields, locks the lattice arrangement of hydrophobic hydrocarbon segments in the interfacial film, and forms a three-dimensional steric network, thereby increasing the interfacial yield stress, enhancing long-term thermodynamic stability, and suppressing stratification and phase coalescence during long-term storage.

Saponin extracts containing at least 88% QS-21 main peak and >3% to 10% 2018 component by UV absorbance at 214 nm, methods for making said extracts, their use as vaccine adjuvants and related aspects.

The invention relates to the technical field of natural product separation engineering, discloses a method for synchronously separating and purifying multiple active components of camellia oleifera shells based on synergistic extraction-gradient desorption-targeted refining, and aims to solve the technical bottleneck that high-purity tannin, proanthocyanidin and tea saponin cannot be efficiently and synchronously obtained from the camellia oleifera shells in the prior art. The core innovation is as follows: 1) an ultrasonic-microwave synergistic extraction parameter matching system is designed, and efficient synergistic dissolution of three active components is realized through precise matching of ultrasonic power and microwave power (0.5-1.7): 1 in combination with a 65-75% ethanol aqueous solution; (2) a three-stage incremental ethanol concentration gradient elution strategy is innovatively adopted, and directed preliminary separation of component groups is realized through step-by-step elution of 20-30% ethanol, 50-60% ethanol and 80-95% ethanol according to polarity differences of the three components; and (3) establishing a targeted refining technology combination, adopting Sephadex LH-20 gel chromatography according to the molecular weight characteristics of proanthocyanidin, and adopting high-speed counter-current chromatography of an ethyl acetate-n-butyl alcohol-water system in a specific ratio according to the structural characteristics of tea saponin homologues to realize deep purification. The continuous synchronous preparation of three active components in the camellia oleifera shells is realized for the first time, the purity of tannin in the obtained product is greater than or equal to 75%, the purity of proanthocyanidin is greater than or equal to 90%, and the purity of tea saponin is greater than or equal to 92% (the proportion of oleanane saponin is greater than or equal to 85%), the utilization rate of raw materials is increased by 30% or above, and the solvent consumption is reduced by 25%. An efficient, economical and environment-friendly integrated technical scheme is provided for high-value utilization of the camellia oleifera shells, and the method has a remarkable industrial application value.

The invention relates to a therapeutic combination, comprising a first proteinaceous molecule comprising a first binding site for binding to a first epitope of a first cell-surface molecule, the first proteinaceous molecule provided with at least one saponin covalently bound to an amino-acid residue of said first proteinaceous molecule, and comprising a second pharmaceutical composition comprising a second proteinaceous molecule different from the first proteinaceous molecule, the second proteinaceous molecule comprising a second binding site for binding to a second epitope of a second cell-surface molecule different from the first cell-surface molecule, and comprising an effector moiety, wherein the second epitope is different from the first epitope. An aspect of the invention is a composition comprising the first proteinaceous molecule and the second proteinaceous molecule of the invention. The invention also relates to a composition or therapeutic combination comprising an antibody—drug conjugate or antibody—oligonucleotide conjugate and the first proteinaceous molecule of the invention. An aspect of the invention relates to a pharmaceutical composition comprising the composition or the antibody—drug conjugate of the invention, and optionally further comprising a pharmaceutically acceptable excipient. The invention also relates to the therapeutic combination or the composition or the antibody—drug conjugate or the pharmaceutical composition of the invention, for use as a medicament. The invention also relates to the therapeutic combination of the invention for use in the treatment or prophylaxis of a cancer.

The application discloses a modified tea saponin-based green antibacterial separation membrane and a preparation method thereof, and belongs to the field of membrane separation technology and functional polymer materials. The method comprises the following steps: a functional monomer with reaction activity and amphiphilicity is prepared by chemically modifying natural tea saponin through a long-chain aliphatic compound or an unsaturated double bond monomer; a functional casting solution is constructed by adopting a copolymerization path (radical copolymerization of a reactive monomer and a matrix monomer) or an anchoring path (physical entanglement of long-chain modified tea saponin and a membrane matrix); a membrane is formed through an NIPS process; and a dense antibacterial layer is formed by using a tea saponin surface self-enrichment effect. The prepared membrane material has an antibacterial rate of > 99% on escherichia coli and staphylococcus aureus, an antibacterial component retention rate of > 90% after continuous operation for 500 hours, and high flux recovery rate and long-acting antibiofouling performance. The application solves the technical problems that natural tea saponin is easy to flow off and has poor compatibility with a traditional membrane matrix, and provides an intrinsic sterilization and physical screening cooperative separation membrane for green water treatment.

This invention discloses a triple inactivated vaccine containing Mycoplasma gallisepticum, Mycoplasma synoviae, and Infectious Laryngotracheitis in chickens, its preparation method, and its application. The technical solution includes inactivated Mycoplasma gallisepticum antigen, inactivated Mycoplasma synoviae antigen, and inactivated Infectious Laryngotracheitis Virus gD protein; wherein the inactivated Mycoplasma gallisepticum antigen is prepared from Mycoplasma gallisepticum strain YBMG1, and the preservation number of Mycoplasma gallisepticum strain YBMG1 is CCTCC M 20261107. This invention rationally combines Mycoplasma gallisepticum antigen, Mycoplasma synoviae antigen, and chicken infectious laryngotracheitis virus gD protein, and uses saponin as an immune enhancer. This effectively reduces the interference of chicken infectious laryngotracheitis virus gD protein on the immune response of Mycoplasma gallisepticum and Mycoplasma synoviae, and the effect is significantly better than other polysaccharide or polypeptide immune enhancers, thereby achieving simultaneous prevention and control of Mycoplasma gallisepticum disease, Mycoplasma synoviae disease, and chicken infectious laryngotracheitis.

A vaccine composition comprising a saponin and an immunostimulatory oligonucleotide and a tumor associated antigen, tumor rejection antigen or antigens associated with tumor support mechanisms, use for prophylaxis or the treatment of cancer.

This invention provides a method for preparing and applying a Pomacea canaliculata (golden apple snail) attractant based on the regulation of feeding and oviposition behaviors, belonging to the field of agricultural and forestry technology. The preparation method involves encapsulating Cyperus rotundus essential oil with β-cyclodextrin to obtain an essential oil inclusion complex; ultra-finely pulverizing glycine, sodium citrate, anhydrous magnesium sulfate, Acorus tatarinowii extract, capsaicin, and peppermint extract into ultra-fine functional powder; preparing microcapsule active powder from tea saponin and azadirachtin using maltodextrin and gum arabic; mixing sweet potato starch hydrolysate, yeast extract, essential oil inclusion complex, ultra-fine functional powder, microcapsule active powder, bentonite, and talc, then adding carboxymethyl cellulose and sodium alginate to form core particles, which are then coated with a coating solution to obtain the Pomacea canaliculata attractant based on the regulation of feeding and oviposition behaviors. This invention constructs an integrated trapping mode of active attraction, continuous residence, and targeted feeding based on the dual behavioral principles of Pomacea canaliculata's feeding preferences and oviposition tropism.

The invention discloses an environment-friendly spray for inhibiting incubation of ampullaria gigas eggs and a use method of the environment-friendly spray. The environment-friendly spray is characterized by comprising chitosan, acetic acid, sodium chloride, tea saponin, an organic silicon additive and a solvent; comprising the following steps: step 1, spraying opportunity; step 2, spraying preparation; step 3, a spraying mode; and step 4, effect verification. The environment-friendly spray takes natural active ingredients as a core, is environment-friendly and residue-free, is friendly to non-target organisms, can accurately inhibit incubation of ampullaria gigas eggs and block expansion of ampullaria gigas population from the source, and is simple in preparation process, convenient to use and suitable for large-area popularization and application.

The application of diosgenin in the preparation of drugs for the prevention or treatment of acute kidney injury falls within the pharmaceutical field. Specifically, this invention discloses that diosgenin, by directly binding to the PIK3CA protein, regulates the PI3K / Akt-NF-κB pathway, inhibiting inflammatory responses, apoptosis, and pyroptosis in renal tubular epithelial cells, thereby alleviating kidney damage. Cellular experiments show that diosgenin at concentrations of 0.1–5 μM can significantly protect renal tubular epithelial cells; animal experiments show that doses of 1–20 mg / kg can significantly improve renal function and reduce pathological damage in mice with acute kidney injury. This invention clarifies a complete chain of evidence from "compound—target—pathway—efficacy," providing a new drug candidate for the treatment of acute kidney injury and offering a paradigm for the mechanistic study of active ingredients in traditional Chinese medicine, contributing to the transformation of traditional Chinese medicine from empirical medicine to evidence-based and molecular medicine.

The invention provides an animal intestinal immune regulation feed additive and feed, and relates to the technical field of animal feed. The animal intestinal immune regulation feed additive provided by the invention is prepared by drying an additive premixed solution, 100 parts by mass of the additive premix liquid comprise 20-40 parts of a plant source silicon aqueous solution with the mass concentration of 30%-40%, 10-20 parts of fructo-oligosaccharide, 5-10 parts of beta-glucan, 2-5 parts of a tea saponin or plant polyphenol aqueous extract, 10-15 parts of calcium amino acid chelate and the balance of a carrier; the particle size of the plant source silicon element is 0.4 nm. The feed additive is good in stability, high in safety, easy to absorb, suitable for animal feed and health care products, especially suitable for animals in the breeding period and animals sensitive to intestinal tracts, and capable of meeting the requirements of the current animals for efficient absorption type nourishment.

The invention discloses cellulase and a method for preparing high-purity camellia saponin from the cellulase, and belongs to the technical field of gene engineering. The amino acid sequence of the cellulase is as shown in SEQ ID No. 1. Wild cellulase is mutated, the 6th amino acid of the amino acid sequence of the wild cellulase is mutated into P from S, the 67th amino acid of the amino acid sequence of the wild cellulase is mutated into A from G, and the cellulase with the amino acid sequence shown in SEQ ID No. 1 is obtained and named as the cellulase mutant S6P / G67A, and the enzyme activity of the cellulase mutant S6P / G67A is remarkably improved; and the enzymolysis efficiency is improved when the camellia seed cakes or the tea seed cakes are subjected to enzymolysis, so that the extraction efficiency of the camellia saponin is improved.

Disclosed herein, are compositions comprising one or more a molecular guidance system (MGS) peptides and a cytotoxic agent. Also described herein, are methods of administering the compositions to patients with cancer.

This invention relates to the field of natural saponin separation and recombination, and particularly to a complex of saponin from *Gleditsia sinensis* and saponin from *Camellia oleifera* and its preparation method. The method includes: 1) extracting saponin from *Gleditsia sinensis* pericarp and *Camellia oleifera* cake, respectively, to obtain crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 2) using macroporous adsorption resin AB-8 as a resin separation column to elute and remove impurities from the crude saponin solutions from *Gleditsia sinensis* and crude saponin from *Camellia oleifera*; 3) eluting the obtained saponin solutions from *Gleditsia sinensis* and *Camellia oleifera* with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-85% ethanol solution, respectively, to obtain saponin components from *Gleditsia sinensis* and saponin components from *Camellia oleifera*; 4) recombining the saponin components from *Gleditsia sinensis* and *Camellia oleifera* to obtain the complex of saponin from *Gleditsia sinensis* and *Camellia oleifera*. This invention can improve the purity and surface activity of natural saponins, overcoming the disadvantage of the high critical micelle concentration of *Camellia oleifera*.

The invention discloses a preparation method and application of a gleditsia sinensis saponin-silver ion antibacterial chelate, and belongs to the technical field of deep processing of natural products. The method comprises the following steps: (1) extracting gleditsia sinensis saponin by using a deep eutectic solvent, dialyzing an extracting solution, purifying by using macroporous resin, and drying to obtain high-purity gleditsia sinensis saponin; and (2) fully dissolving the prepared gleditsia sinensis saponin as a raw material in distilled water, adding silver nitrate to perform an ultrasonic chelation reaction, dialyzing after chelation is completed, centrifuging dialysate, taking supernatant, and freeze-drying to obtain the gleditsia sinensis saponin-silver ion antibacterial chelate which can effectively inhibit escherichia coli and staphylococcus aureus. The method is easy to operate, the efficiency of chelating the gleditsia sinensis saponin with the silver ions is high, the chelate is good in stability, and the gleditsia sinensis saponin has huge potential in the application aspect of serving as a green, environment-friendly, safe and efficient antibacterial functional surfactant.

The invention discloses an animal feather keratin extraction method which comprises the following steps: (1) mixing pretreated animal feathers with a tea saponin solution, and stirring to obtain degreased feathers; mixing the degreased feathers with an ethanol solution, and performing ultrasonic treatment to obtain loose feathers; (2) preparing liquid drops from the water phase and the oil phase through a micro-fluidic chip process, standing and curing the collected liquid drops, demulsifying and cleaning to obtain sodium alginate-CaCl2 microspheres; (3) mixing the loose feathers, water, the sodium alginate-CaCl2 microspheres and keratinase, then adjusting the pH value, and carrying out enzymolysis; finally, taking supernate; and (4) adjusting the pH value of the supernate, precipitating, dialyzing the precipitated mixed solution, and freeze-drying to obtain the animal feather keratin. According to the method disclosed by the invention, the slow release of calcium ions is realized by adding the sodium alginate-CaCl2 microspheres, so that the enzyme utilization rate is improved.

The invention discloses an enzymolysis gleditsia sinensis saponin-copper chelate as well as a preparation method and application thereof in prevention and treatment of citrus canker, and belongs to the technical field of crop prevention and treatment. The preparation method comprises the following steps: extracting gleditsia sinensis saponin, purifying the gleditsia sinensis saponin, carrying out enzymatic degradation on the gleditsia sinensis saponin, and then adding CuSO4. 5H2O to carry out chelation reaction to obtain the enzymolysis gleditsia sinensis saponin-copper chelate. The initial contact angle of an aqueous solution of the chelate on citrus leaves is small, the chelate can be rapidly wetted and spread on the leaves, the deposition effect is good, the chelate has excellent leaf adhesion performance and anti-scouring capacity, meanwhile, the enzymolysis gleditsia sinensis saponin-copper chelate has excellent inhibitory activity on xanthomonas carpesium, and the enzymolysis gleditsia sinensis saponin-copper chelate can be used for preparing the citrus leaf-copper chelate. The compound can be used as a green, environment-friendly, safe and efficient bactericide, and has excellent application potential in the aspect of citrus canker prevention and control.

The application provides a serreia that can degrade tea saponin efficiently and application thereof in fermentation of oil tea chaff feed, and relates to the field of microbial biotechnology and feed processing.The application discloses a serreia that can degrade tea saponin efficiently, namely, serreia NCUF-1, and application thereof.The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20253034.The serreia NCUF-1 is compounded with lactobacillus plantarum at a specific ratio, inoculated into oil tea chaff, and subjected to mixed bacteria solid state fermentation, so that tea saponin in the oil tea chaff can be degraded efficiently, the degradation rate of tea saponin in the oil tea chaff reaches 40% after three days of fermentation; meanwhile, the product quality is significantly improved, the protein content is stably above 8%, the total acid content is increased to more than 2.5 times of the raw material, at least 13 kinds of beneficial flavor substances such as linalool and phenylethanol are synthesized, and the palatability is effectively improved.The application provides a new strain that is efficient and safe for biological detoxification and high-value feed utilization of oil tea chaff, and provides an industrialized fermentation technical scheme.