113 results about "Saporin" patented technology

The invention provides an organic molecule cytoplasm delivery carrier as well as a preparation method and application thereof, the compound for cytoplasm delivery has the characteristic of fat-soluble aggregation, and can form electrostatic adsorption with small molecule protein goods such as RNase A (13.7 KDa), Trypsin (24KDa) and Saporin (32.8 KDa), medium molecule protein goods such as HRP (40KDa) and macromolecular protein goods such as beta-Gal (430KDa), so that the compound for cytoplasm delivery can be used for preparing a lipid-soluble protein carrier. And furthermore, transmembrane transportation is formed through the strong fat solubility property, so that the protection and intracellular delivery of carried protein molecules are realized. According to the delivery mechanism, cargo molecules can quickly penetrate through cell membranes, and carried protein molecules can enter cytoplasm to exert functions without release operation. Development of the organic molecule cytoplasm delivery carrier is expected to assist potential protein therapies with low bioavailability and off-target toxicity to complete clinical transformation, which is crucial to tumor therapy, gene therapy and the like requiring precise local administration.

The invention particularly relates to a curcumin delivery system based on small extracellular vesicles, a preparation method and application. Curcumin has the defects of poor water solubility, poor chemical stability and insufficient targeting, in order to improve the bioavailability of curcumin, the curcumin delivery system based on small extracellular vesicles provided by the invention adopts RGD peptide surface modified sEVs as a carrier to coat curcumin, and saponin is used for assisting ultrasonic drug loading, so that the drug loading rate of curcumin is improved. According to verification of the invention, the delivery system can effectively improve the in-vitro release performance of the curcumin preparation and realize controlled release and slow release. Meanwhile, the delivery system effectively improves apoptosis induction of curcumin to glioma cells, the blood-brain barrier penetrating capacity of the curcumin delivery system is improved, and the treatment effect on brain glioma is expected to be improved.

The invention provides a biological fermentation feed for enhancing immunity of aquatic animals and a preparation method of the biological fermentation feed, and relates to the technical field of aquaculture. The biological fermentation feed is prepared by compounding fish bone meal, a rice bran fermentation material, soybean meal, a compound fermentation material, visceral meal, orange peel powder, corn starch, compound vitamins, tea saponin and protocatechuic acid. According to the present invention, the defects in the prior art are overcome, the weight gain growth of the aquatic product is comprehensively ensured through the compound addition of the microbial components of the aquatic product feed and the regulation and control of other components, the immunity of the aquatic product is further improved, and the artificial breeding benefit of the aquatic product is comprehensively ensured.

This invention discloses tea saponin E3, its isolation and purification method, and its application as a killer of moon jellyfish polyps, belonging to the field of natural product chemistry. The structure of tea saponin E3 is shown below. Derived from plants, it is a natural triterpenoid saponin compound. Compared with tea saponin, it exhibits stronger killing activity against moon jellyfish polyps. Furthermore, its single component and well-defined structure facilitate standardized application and evaluation of potential ecological risks in the biocontrol of moon jellyfish polyps.

The present invention refers to: a) at least one peptide recognizing tumor endothelial cell markers, the peptide being preferably: —an αv-integrin ligand, preferably a ligand of αvβ3, βvβ5, αvβ8, α5β1 and / or αvβ6 integrin, more preferably the peptide includes a sequence having a RGD motif or of a CgA sequence or—a ligand of CD 13 receptor, more preferably the ligand includes a sequence having a NGR motif or functional fragments or derivatives or a biologically active variant thereof and b) saporin or functional fragments, derivatives or a biologically active variant thereof.

The invention discloses a tea saponin bactericide. The active components in the bactericide comprise tea saponin, a microbial complex microbial inoculant and an auxiliary agent. By adding the paenibacillus, the bacillus amyloliquefaciens, the gluconacetobacter xylinum and the tea saponin into the bactericide, the activity of the navel orange colletotrichum gloeosporioides and the navel orange alternaria brassicae can be effectively inhibited.

The invention discloses a method for analyzing phenolic components in perilla leaves based on micellar electrokinetic chromatography. The preparation method comprises the following steps: grinding perilla leaves, and adding a methanol solution to obtain a perilla leaf extracting solution; uniformly mixing tea saponin, borax and distilled water, performing ultrasonic treatment, filtering, and adjusting the pH value to 8.0-8.4 to obtain a buffer solution; the method comprises the following steps: equivalently adding ten phenol standard substances into methanol, and mixing to obtain a standard substance solution; the perilla leaf extracting solution and the mixed standard substance solution are subjected to centrifugal treatment, supernatant is taken and subjected to electrokinetic chromatographic separation with the prepared buffer solution, and a perilla leaf electrophoresis chromatogram and a standard substance electrophoresis chromatogram are obtained; and finally, performing quantitative analysis on phenolic substances in the perilla leaves according to peak areas of the standard product electrophoresis chromatogram and the perilla leaf electrophoresis chromatogram. The natural tea saponin is applied to the micellar electrokinetic chromatography technology and used for separating the phenolic compounds in the perilla leaves, and a novel method capable of simply, rapidly, greenly and effectively analyzing the phenolic compounds in the perilla leaves is provided.

The present invention discloses a repair functional composition HAmella containing sodium hyaluronate and tremella polysaccharide, belonging to the field of biomedical technology. The preparation method includes: mixing sodium hyaluronate and tremella polysaccharide, dissolving them in water to prepare a polysaccharide aqueous solution; wherein, the molecular weight of the sodium hyaluronate is 100 - 300 KD, and the molecular weight of the tremella polysaccharide is 50 - 200 KD; dissolving saponin in water to prepare an aqueous phase, and then adding camellia oil to the aqueous phase to prepare a camellia oil microemulsion; mixing the polysaccharide aqueous solution and the camellia oil microemulsion evenly, and spray drying to obtain a gastric mucosa protectant. The preparation method of the protectant of the present invention is simple, shows excellent performance in gastrointestinal protection, and has an injury inhibition rate of about 50% in an ethanol-induced gastric ulcer model.

In one aspect, the disclosure relates to immunogenic compositions comprising a semisynthetic saponin adjuvant (VSA) including, but not limited to, VSA-1 and VSA-2, derived from a Momordica saponin and at least one antigen. In a further aspect, the immunogenic compositions can include other known adjuvants, emulsifiers, and / or surface-active agents and can be formulated as liposomes, micelles, emulsions, and the like. Also disclosed are methods of eliciting effective immune responses to viral, bacterial, protozoal, and neoplastic diseases using the disclosed immunogenic compositions.

The invention discloses a method for screening and extracting DES of tea saponin based on molecular simulation and machine learning, and relates to the technical field of tea saponin extraction.The method comprises the steps that tea saponin, cellulose, a hydrogen bond donor, a hydrogen bond receptor and a DES molecular model are constructed and optimized, and a molecular polarity index is obtained; the method comprises the following steps: generating a topology, obtaining a trajectory and energy through MD simulation, and calculating E (TeaS-DES), E (TeaS-Cell) and SI; and measuring the extraction rate, combining the molecular polarity index, SI, process parameters and the like to construct a data set to train a machine learning model, predicting the extraction rate, and locking the optimal DES and process. According to the method, DES screening can be directly guided through simulated energy and structure descriptors, the number of experiments is reduced, and the research and development period is shortened.

The invention relates to the technical field of natural product separation, and provides high-purity tea saponin and application thereof in poultry virus disease prevention and treatment, and a preparation method of the high-purity tea saponin, the preparation method comprises the following steps: S1, adding tea seed meal powder into an extracting agent, mixing, and filtering to obtain an extracting solution; s2, purifying the extracting solution to obtain tea saponin; the extracting agent comprises a component A and a component B in a molar ratio of 1: (1-8); the component A comprises one or two of alanine and histidine; the component B comprises methylurea. According to the technical scheme, the problem that the purity and the yield are low when tea saponin is extracted from plants in the prior art is solved.

The invention discloses application of a saponin enzymolysis product in prevention and treatment of plutella xylostella, and belongs to the technical field of biopesticides. According to the method, gleditsia sinensis saponin is degraded by using biological enzyme, and the obtained saponin degradation product has excellent food refusal, contact toxicity and stomach toxicity activity on plutella xylostella. The raw materials are obtained from natural plants, the preparation method is green and safe, the activity of degradation products is high, use of chemical pesticides can be reduced, degradation can be achieved in the natural environment, and a novel green prevention and control agent and technology can be provided for prevention and control of plutella xylostella.

The present application relates to the technical field of biotechnology, and discloses a C26 hydroxylase for improving the biosynthesis efficiency of diosgenin, a coding gene, a preparation method and an application. The protein of the C26 hydroxylase is any one of the following a), b) or c): a) a protein with an amino acid sequence shown in SEQ ID NO. 2; b) a fusion protein obtained by connecting a tag to the N terminal and / or C terminal of the protein shown in SEQ ID NO. 2; and c) a protein with the same function obtained by substitution, deletion and / or addition of one or more amino acid residues to the amino acid sequence shown in SEQ ID NO. 2. The C26 hydroxylase provided by the present application can improve the biosynthesis efficiency of diosgenin, and provides a key gene resource for producing diosgenin by using an enzyme method.

The invention discloses a dog head roundworm rTcMUCs-PLGA nano vaccine as well as a preparation method and application thereof, and the dog head roundworm rTcMUCs-PLGA nano vaccine is characterized in that the nano vaccine takes PLGA nano microspheres as a carrier and is loaded with TcMUC-2 and / or TcMUC-3 recombinant protein. Animal protection test results show that compared with a PBS and PLGA control group, the number of L3-stage larvae of the dog head roundworm in livers and lungs of mice inoculated with the rTcMUC-2-PLGA and the rTcMUC-3-PLGA in an immune group is obviously reduced, the number of L3-stage larvae of the dog head roundworm in the immune group is 55.31% and 53.61% compared with the number of L3-stage larvae of mice inoculated with the rTcMUC-2 and the number of L3-stage larvae of the mice inoculated with the rTcMUC-3 in an immune group taking saponin as an adjuvant, and after the rTcMUC-2 and the rTcMUC-3 are coated with the PLGA as a nano-carrier, the L3-stage larvae of the dog head Compared with the prior art, the PLGA nano-vaccine has the advantages that the immune frequency is reduced from three times to two times, the worm reduction rates respectively reach 65.13% and 55.9%, and the result proves that the PLGA nano-vaccine can be used as an adjuvant of recombinant protein to reduce the immune cycle and induce a host to generate better immune protection force to inhibit invasion and migration of larvae of the dog head roundworm.

The invention discloses a multi-scene deodorizing detergent composition and a preparation method thereof, and relates to the technical field of daily chemical industry. The multi-scene deodorizing detergent composition provided by the invention is prepared from the following core components: alkyl glycoside, sodium lauroamphoacetate, tea saponin, hydroxypropyl-beta-cyclodextrin, chitosan, compound enzyme, sodium cinnamate, dragon fruit extract, humulus lupulus extract and the like. Through the synergistic effect of all the components, the composition realizes integration of multiple effects: the irritation is remarkably reduced and the skin barrier is maintained while efficient decontamination is realized; an adsorption-degradation system formed by hydroxypropyl-beta-cyclodextrin, chitosan and compound enzyme can radically remove peculiar smell and efficiently degrade pesticide residues; the chitosan and the pitaya extract synergistically chelate heavy metal ions; and components such as sodium cinnamate have natural whitening and broad-spectrum preservative functions. The technical limitation that a traditional product is single in function is broken through, and a comprehensive and safe washing solution with a positive skin care effect is provided.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The present disclosure relates to antigen-binding molecules that bind to human and feline Human Trophoblast Cell Surface Antigen 2 (TROP2). The specific embodiments relate to antibody-drug conjugates (ADC) comprising saporin, mertansine and MMAE and chimeric antigen receptors (CAR) comprising said antigen-binding molecules for use in treatment of cancer.

The invention discloses a method for converting furan steroid saponin in a plant body into spiral steroid saponin and producing saponin, which comprises the following steps of: cleaning and selecting fresh leaves of related plants, cutting into pieces, soaking in cold water twice, and combining solutions; performing suction filtration or centrifugation; obtaining a crude enzyme solution; the method comprises the following steps of: adding the saponin into mixed slurry of dioscorea zingiberensis and the like, converting for 1-72 hours, judging whether conversion is complete or not by using a reagent E and a reagent A in combination with a thin-layer chromatography method, reproducing saponin, and comparing the saponin with saponin produced by a conventional pre-fermentation acid hydrolysis method. The method disclosed by the invention has the advantages that the furan steroid saponin in the dioscorea rhizome group plants is converted into the spiral steroid saponin by utilizing the leaching solution of the fresh leaves of the dioscorea rhizome group plants in China, and the saponin is produced and prepared. Compared with the traditional process, the spiral steroid saponin has the advantages of higher yield, lower acid consumption, lower pollution and shorter process time.

The invention provides a breeding method for improving disease resistance of carassius auratus var pengze myxosporidium. The breeding method comprises the following steps of S1, breeding environment treatment, S2, healthy fry selection and purchase, S3, fry disinfection, S4, fry putting, S5, feeding management, S6, water quality management, S7, disease prevention and detection and S8, emergency treatment. In the fry disinfection link, a biological disinfectant composed of tea saponin, a photosensitive component, carvacrol, artesunate and choline-lactic acid ionic liquid is used for soaking carassius auratus var pengze fry, meanwhile, LED light irradiation is used for assistance, the disinfection components can jointly play a role after penetrating into myxosporidium, growth and reproduction of the myxosporidium are inhibited, and therefore the carassius auratus var pengze fry can be prevented from being damaged by the carassius auratus var pengze. Therefore, the effect of effectively killing myxosporidia is achieved. In the emergency treatment link, an insect-resistant agent composed of a glycan-mercaptosuccinic acid conjugate, antibacterial peptide, artesunate, nano-zinc oxide, triptolide and carvacrol is used, and through the synergistic effect of the components, multi-target efficient killing of myxosporidium is achieved.

The invention discloses application of alkalized tea saponin as a talc inhibitor, and belongs to the technical field of mineral separation. The molecular structural formula of the alkalized tea saponin is shown in the specification, wherein R is a sugar body unit; m is a cation; the alkalized tea saponin has a selective inhibition effect on flotation of talc, and is particularly suitable for flotation separation of metal sulfide ore (such as molybdenite, chalcopyrite, galena and sphalerite) and talc.

The invention relates to the technical field of biochemical wastewater treatment and automatic control, in particular to a comprehensive utilization and treatment system for dioscorea zingiberensis saponin wastewater. The data acquisition module comprises a data acquisition module, a prediction module, a quantification module, a risk hedging module and a control execution module; the system estimates key enzyme activity by adopting a non-linear model by collecting a water inlet fingerprint vector and a reactor state; the core of the method is that a system safety distance is determined according to enzyme activity and flora health degree, and control weights of three targets of collapse risk, effluent compliance and resource recovery are dynamically adjusted according to the system safety distance; when the safe distance changes, the system executes compound control through a multi-objective optimization algorithm; according to the method, prospective early warning of the core biochemical state is realized, and the defect of response lag of a traditional method is overcome.

The present invention belongs to the technical field of extraction and purification of fat-soluble chemical components, and particularly relates to a method for extracting and purifying dihydroquercetin from larch xylem. The present invention uses tea saponin aqueous micelles (tea saponin aqueous solution) to greenly extract dihydroquercetin from larch xylem, and then, according to the interfacial charge characteristics of the micelles formed by tea saponin and dihydroquercetin in the aqueous solution, utilizes the charge characteristics of chitosan to directionally enrich dihydroquercetin in the extract, and combines anti-solvent biphasic crystallization and water-solvent single-phase recrystallization to purify dihydroquercetin. The extraction and purification method provided by the present invention has a high extraction rate of dihydroquercetin and a high purity of the obtained pure product, and has the characteristics of simple operation, green safety, high efficiency and low cost. It not only improves the utilization rate of larch resources, but also has great significance for giving play to the high-value application of dihydroquercetin.

The invention discloses a fish feed containing corn polysaccharide, and belongs to the technical field of fish culture. The fish feed comprises fish meal, krill meal, casein, soybean meal, cottonseed protein, plant starch, oil, cellulose, a multi-vitamin and multi-mineral premix, a calcium element additive, choline chloride, amino acid and corn polysaccharide fermentation liquor. Wherein the corn polysaccharide fermentation liquor is obtained by fermenting a corn polysaccharide solution, fish soluble pulp and tea saponin through clostridium tyrobutyricum. The corn polysaccharide fermentation liquor in the fish feed contains various beneficial metabolites and active ingredients, so that the nutritional value of the feed can be improved, the intestinal health of fishes can be improved, the immune function of the fishes can be enhanced, the digestibility and the utilization rate of the fishes to the feed can be improved, the growth performance of the fishes can be further improved, and the culture benefits can be improved.

This invention relates to the field of natural product separation and recombination, and particularly to a recombinant saponin from *Gleditsia sinensis* and its preparation method. The method includes: 1) crude saponin extraction: water extraction of *Gleditsia sinensis* pericarp to obtain a crude saponin solution; 2) impurity removal: loading the crude saponin aqueous solution into macroporous adsorption resin AB-8 and eluting to remove impurities; 3) saponin component separation: eluting sequentially with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-80% ethanol solution to obtain S30, S45, S60, and S75 saponin components, respectively; 4) saponin hydrolysis: hydrolyzing the S45 saponin component to obtain hydrolyzed saponin; 5) saponin component recombination: recombining the saponin components obtained in steps 3) and 4) with the hydrolyzed saponin to obtain recombinant saponin from *Gleditsia sinensis*. This invention enables saponin purification and improves the surface activity of *Gleditsia sinensis*.

We provide novel, specific cytotoxic drug therapies with minimal side effects. [Solution] A composition for a membrane-permeable complex for transport across a lipid membrane is provided, comprising one or more molecular induction system (MGS) peptides and a cytotoxic agent. A method for treating cancer is also provided, comprising administering this composition to a cancer patient.

The invention discloses an environment-friendly coal mine dust falling agent and a preparation method thereof. The coal mine dust falling agent is prepared from peanut shell cellulose and a surfactant. The surfactant is one or more of tea saponin, sucrose ester, soapberry and lipopeptide. The preparation method of the coal mine dust reducing agent comprises the following steps: S1, extracting peanut shell cellulose from peanut shells by adopting an enzyme method; and S2, compounding the peanut shell cellulose and a surfactant to obtain the dust falling agent. Cellulose in peanut shells is extracted through an enzyme method and is compounded with surfactants such as biological tea saponin, sucrose ester, soapberry and lipopeptide, and the environment-friendly coal mine dust falling agent is prepared. The method has the advantages of being simple in process, easy to operate, low in raw material cost, biodegradable after spraying and dust falling, free of secondary pollution and good in application prospect in the field of coal mine dust falling.

Provided are variants of saponins that are found in Quillaja saponaria (QS) Molina tree bark that are chemically modified to distinguish them from naturally occurring parent saponins. The modified saponins have increased adjunctive activity compared to the unmodified parent saponins. Defined structures allow for comparisons of the modifying groups with respect to their respective adjunct activity and permit characterized vaccine formulations that have one or more defined saponins.

The invention discloses a method for removing drug pollutants in water by compounding tea saponin and moringa seed protein. The method comprises the following steps: sequentially adding tea saponin and moringa seed protein into a reactor containing medicine wastewater according to a mass ratio, and ensuring that the tea saponin and the moringa seed protein are uniformly dispersed; adjusting the pH value of the wastewater to a proper range, and keeping the temperature under a proper condition; quickly stirring for a period of time, and then slowly stirring to promote full combination of the drug pollutants, the tea saponin and the moringa seed protein; and after standing and precipitating, separating the precipitate from the wastewater by a filtering or centrifuging method so as to realize drug removal. The process is simple, convenient and environment-friendly, the tea saponin and the moringa seed protein extracted from the tea leaves and the moringa tree seeds are utilized, introduction of harmful chemical components is avoided, and the method is harmless to the environment and safe to a human body. Meanwhile, the method has a remarkable cost advantage compared with a traditional chemical flocculant while effectively removing drug pollutants in water.

The invention relates to the technical field of aviation plant protection aids, and discloses an aviation plant protection aid containing natural plant polysaccharides and a preparation method of the aviation plant protection aid. Constructing a dynamic self-repairing system of a laccase catalysis phenol crosslinking component, a peroxidase catalysis polysaccharide coupling component and a cellulose degradation product repairing storage material, and a biodegradation regulation and control system integrating a cellulase-xylanase compound enzyme component, a tea saponin regulation component and a peptone nutrition component; the functions of enhancing leaf surface attachment, dynamic self-repairing and environment-friendly degradation of the auxiliary agent are realized through multiple synergistic effects. The technical problems that a traditional aviation plant protection auxiliary is insufficient in leaf surface adhesive force, damage cannot be automatically repaired, and environment residues are accumulated are solved, stable adhesion of liquid medicine under the severe weather condition, automatic repairing capacity after film damage and complete biodegradation within 30 days after the pesticide effect period is finished are achieved, and the effect is achieved. And the reliability, persistence and environmental friendliness of aviation plant protection are remarkably improved.

The present invention provides a kind of breeding method that improves the disease resistance of Pengze crucian carp myxosporeans, comprising the following steps: S1. breeding environment treatment, S2. purchasing healthy fry, S3. fry disinfection, S4. fry delivery, S5. feeding management, S6. water quality management, S7. disease prevention and detection, S8. emergency treatment. In the fry disinfection link, Pengze crucian carp fry are soaked by using a biological disinfectant composed of tea saponin, photosensitizing component, carvacrol, artesunate, and choline-lactic acid ionic liquid, and supplemented with LED light irradiation. These disinfecting components can work together after penetrating into the inside of the myxosporeans, inhibiting their growth and reproduction, thereby achieving the effect of effectively killing the myxosporeans. In the emergency treatment link, the insecticide composed of polysaccharide-mercaptosuccinic acid conjugate, antimicrobial peptide, artesunate, nano zinc oxide, triptolide, and carvacrol is used, and through the synergistic effect between the components, multi-target efficient killing of myxosporeans is achieved.