99 results about "Saporin" patented technology

The invention provides an organic molecule cytoplasm delivery carrier as well as a preparation method and application thereof, the compound for cytoplasm delivery has the characteristic of fat-soluble aggregation, and can form electrostatic adsorption with small molecule protein goods such as RNase A (13.7 KDa), Trypsin (24KDa) and Saporin (32.8 KDa), medium molecule protein goods such as HRP (40KDa) and macromolecular protein goods such as beta-Gal (430KDa), so that the compound for cytoplasm delivery can be used for preparing a lipid-soluble protein carrier. And furthermore, transmembrane transportation is formed through the strong fat solubility property, so that the protection and intracellular delivery of carried protein molecules are realized. According to the delivery mechanism, cargo molecules can quickly penetrate through cell membranes, and carried protein molecules can enter cytoplasm to exert functions without release operation. Development of the organic molecule cytoplasm delivery carrier is expected to assist potential protein therapies with low bioavailability and off-target toxicity to complete clinical transformation, which is crucial to tumor therapy, gene therapy and the like requiring precise local administration.

The invention particularly relates to a curcumin delivery system based on small extracellular vesicles, a preparation method and application. Curcumin has the defects of poor water solubility, poor chemical stability and insufficient targeting, in order to improve the bioavailability of curcumin, the curcumin delivery system based on small extracellular vesicles provided by the invention adopts RGD peptide surface modified sEVs as a carrier to coat curcumin, and saponin is used for assisting ultrasonic drug loading, so that the drug loading rate of curcumin is improved. According to verification of the invention, the delivery system can effectively improve the in-vitro release performance of the curcumin preparation and realize controlled release and slow release. Meanwhile, the delivery system effectively improves apoptosis induction of curcumin to glioma cells, the blood-brain barrier penetrating capacity of the curcumin delivery system is improved, and the treatment effect on brain glioma is expected to be improved.

This invention discloses tea saponin E3, its isolation and purification method, and its application as a killer of moon jellyfish polyps, belonging to the field of natural product chemistry. The structure of tea saponin E3 is shown below. Derived from plants, it is a natural triterpenoid saponin compound. Compared with tea saponin, it exhibits stronger killing activity against moon jellyfish polyps. Furthermore, its single component and well-defined structure facilitate standardized application and evaluation of potential ecological risks in the biocontrol of moon jellyfish polyps.

The present invention refers to: a) at least one peptide recognizing tumor endothelial cell markers, the peptide being preferably: —an αv-integrin ligand, preferably a ligand of αvβ3, βvβ5, αvβ8, α5β1 and / or αvβ6 integrin, more preferably the peptide includes a sequence having a RGD motif or of a CgA sequence or—a ligand of CD 13 receptor, more preferably the ligand includes a sequence having a NGR motif or functional fragments or derivatives or a biologically active variant thereof and b) saporin or functional fragments, derivatives or a biologically active variant thereof.

The invention discloses a method for analyzing phenolic components in perilla leaves based on micellar electrokinetic chromatography. The preparation method comprises the following steps: grinding perilla leaves, and adding a methanol solution to obtain a perilla leaf extracting solution; uniformly mixing tea saponin, borax and distilled water, performing ultrasonic treatment, filtering, and adjusting the pH value to 8.0-8.4 to obtain a buffer solution; the method comprises the following steps: equivalently adding ten phenol standard substances into methanol, and mixing to obtain a standard substance solution; the perilla leaf extracting solution and the mixed standard substance solution are subjected to centrifugal treatment, supernatant is taken and subjected to electrokinetic chromatographic separation with the prepared buffer solution, and a perilla leaf electrophoresis chromatogram and a standard substance electrophoresis chromatogram are obtained; and finally, performing quantitative analysis on phenolic substances in the perilla leaves according to peak areas of the standard product electrophoresis chromatogram and the perilla leaf electrophoresis chromatogram. The natural tea saponin is applied to the micellar electrokinetic chromatography technology and used for separating the phenolic compounds in the perilla leaves, and a novel method capable of simply, rapidly, greenly and effectively analyzing the phenolic compounds in the perilla leaves is provided.

The present invention discloses a repair functional composition HAmella containing sodium hyaluronate and tremella polysaccharide, belonging to the field of biomedical technology. The preparation method includes: mixing sodium hyaluronate and tremella polysaccharide, dissolving them in water to prepare a polysaccharide aqueous solution; wherein, the molecular weight of the sodium hyaluronate is 100 - 300 KD, and the molecular weight of the tremella polysaccharide is 50 - 200 KD; dissolving saponin in water to prepare an aqueous phase, and then adding camellia oil to the aqueous phase to prepare a camellia oil microemulsion; mixing the polysaccharide aqueous solution and the camellia oil microemulsion evenly, and spray drying to obtain a gastric mucosa protectant. The preparation method of the protectant of the present invention is simple, shows excellent performance in gastrointestinal protection, and has an injury inhibition rate of about 50% in an ethanol-induced gastric ulcer model.

In one aspect, the disclosure relates to immunogenic compositions comprising a semisynthetic saponin adjuvant (VSA) including, but not limited to, VSA-1 and VSA-2, derived from a Momordica saponin and at least one antigen. In a further aspect, the immunogenic compositions can include other known adjuvants, emulsifiers, and / or surface-active agents and can be formulated as liposomes, micelles, emulsions, and the like. Also disclosed are methods of eliciting effective immune responses to viral, bacterial, protozoal, and neoplastic diseases using the disclosed immunogenic compositions.

The invention discloses a method for screening and extracting DES of tea saponin based on molecular simulation and machine learning, and relates to the technical field of tea saponin extraction.The method comprises the steps that tea saponin, cellulose, a hydrogen bond donor, a hydrogen bond receptor and a DES molecular model are constructed and optimized, and a molecular polarity index is obtained; the method comprises the following steps: generating a topology, obtaining a trajectory and energy through MD simulation, and calculating E (TeaS-DES), E (TeaS-Cell) and SI; and measuring the extraction rate, combining the molecular polarity index, SI, process parameters and the like to construct a data set to train a machine learning model, predicting the extraction rate, and locking the optimal DES and process. According to the method, DES screening can be directly guided through simulated energy and structure descriptors, the number of experiments is reduced, and the research and development period is shortened.

The invention discloses application of a saponin enzymolysis product in prevention and treatment of plutella xylostella, and belongs to the technical field of biopesticides. According to the method, gleditsia sinensis saponin is degraded by using biological enzyme, and the obtained saponin degradation product has excellent food refusal, contact toxicity and stomach toxicity activity on plutella xylostella. The raw materials are obtained from natural plants, the preparation method is green and safe, the activity of degradation products is high, use of chemical pesticides can be reduced, degradation can be achieved in the natural environment, and a novel green prevention and control agent and technology can be provided for prevention and control of plutella xylostella.

The present application relates to the technical field of biotechnology, and discloses a C26 hydroxylase for improving the biosynthesis efficiency of diosgenin, a coding gene, a preparation method and an application. The protein of the C26 hydroxylase is any one of the following a), b) or c): a) a protein with an amino acid sequence shown in SEQ ID NO. 2; b) a fusion protein obtained by connecting a tag to the N terminal and / or C terminal of the protein shown in SEQ ID NO. 2; and c) a protein with the same function obtained by substitution, deletion and / or addition of one or more amino acid residues to the amino acid sequence shown in SEQ ID NO. 2. The C26 hydroxylase provided by the present application can improve the biosynthesis efficiency of diosgenin, and provides a key gene resource for producing diosgenin by using an enzyme method.

The invention discloses a dog head roundworm rTcMUCs-PLGA nano vaccine as well as a preparation method and application thereof, and the dog head roundworm rTcMUCs-PLGA nano vaccine is characterized in that the nano vaccine takes PLGA nano microspheres as a carrier and is loaded with TcMUC-2 and / or TcMUC-3 recombinant protein. Animal protection test results show that compared with a PBS and PLGA control group, the number of L3-stage larvae of the dog head roundworm in livers and lungs of mice inoculated with the rTcMUC-2-PLGA and the rTcMUC-3-PLGA in an immune group is obviously reduced, the number of L3-stage larvae of the dog head roundworm in the immune group is 55.31% and 53.61% compared with the number of L3-stage larvae of mice inoculated with the rTcMUC-2 and the number of L3-stage larvae of the mice inoculated with the rTcMUC-3 in an immune group taking saponin as an adjuvant, and after the rTcMUC-2 and the rTcMUC-3 are coated with the PLGA as a nano-carrier, the L3-stage larvae of the dog head Compared with the prior art, the PLGA nano-vaccine has the advantages that the immune frequency is reduced from three times to two times, the worm reduction rates respectively reach 65.13% and 55.9%, and the result proves that the PLGA nano-vaccine can be used as an adjuvant of recombinant protein to reduce the immune cycle and induce a host to generate better immune protection force to inhibit invasion and migration of larvae of the dog head roundworm.

The invention discloses a multi-scene deodorizing detergent composition and a preparation method thereof, and relates to the technical field of daily chemical industry. The multi-scene deodorizing detergent composition provided by the invention is prepared from the following core components: alkyl glycoside, sodium lauroamphoacetate, tea saponin, hydroxypropyl-beta-cyclodextrin, chitosan, compound enzyme, sodium cinnamate, dragon fruit extract, humulus lupulus extract and the like. Through the synergistic effect of all the components, the composition realizes integration of multiple effects: the irritation is remarkably reduced and the skin barrier is maintained while efficient decontamination is realized; an adsorption-degradation system formed by hydroxypropyl-beta-cyclodextrin, chitosan and compound enzyme can radically remove peculiar smell and efficiently degrade pesticide residues; the chitosan and the pitaya extract synergistically chelate heavy metal ions; and components such as sodium cinnamate have natural whitening and broad-spectrum preservative functions. The technical limitation that a traditional product is single in function is broken through, and a comprehensive and safe washing solution with a positive skin care effect is provided.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The present disclosure relates to antigen-binding molecules that bind to human and feline Human Trophoblast Cell Surface Antigen 2 (TROP2). The specific embodiments relate to antibody-drug conjugates (ADC) comprising saporin, mertansine and MMAE and chimeric antigen receptors (CAR) comprising said antigen-binding molecules for use in treatment of cancer.

The invention discloses a method for converting furan steroid saponin in a plant body into spiral steroid saponin and producing saponin, which comprises the following steps of: cleaning and selecting fresh leaves of related plants, cutting into pieces, soaking in cold water twice, and combining solutions; performing suction filtration or centrifugation; obtaining a crude enzyme solution; the method comprises the following steps of: adding the saponin into mixed slurry of dioscorea zingiberensis and the like, converting for 1-72 hours, judging whether conversion is complete or not by using a reagent E and a reagent A in combination with a thin-layer chromatography method, reproducing saponin, and comparing the saponin with saponin produced by a conventional pre-fermentation acid hydrolysis method. The method disclosed by the invention has the advantages that the furan steroid saponin in the dioscorea rhizome group plants is converted into the spiral steroid saponin by utilizing the leaching solution of the fresh leaves of the dioscorea rhizome group plants in China, and the saponin is produced and prepared. Compared with the traditional process, the spiral steroid saponin has the advantages of higher yield, lower acid consumption, lower pollution and shorter process time.

The invention provides a breeding method for improving disease resistance of carassius auratus var pengze myxosporidium. The breeding method comprises the following steps of S1, breeding environment treatment, S2, healthy fry selection and purchase, S3, fry disinfection, S4, fry putting, S5, feeding management, S6, water quality management, S7, disease prevention and detection and S8, emergency treatment. In the fry disinfection link, a biological disinfectant composed of tea saponin, a photosensitive component, carvacrol, artesunate and choline-lactic acid ionic liquid is used for soaking carassius auratus var pengze fry, meanwhile, LED light irradiation is used for assistance, the disinfection components can jointly play a role after penetrating into myxosporidium, growth and reproduction of the myxosporidium are inhibited, and therefore the carassius auratus var pengze fry can be prevented from being damaged by the carassius auratus var pengze. Therefore, the effect of effectively killing myxosporidia is achieved. In the emergency treatment link, an insect-resistant agent composed of a glycan-mercaptosuccinic acid conjugate, antibacterial peptide, artesunate, nano-zinc oxide, triptolide and carvacrol is used, and through the synergistic effect of the components, multi-target efficient killing of myxosporidium is achieved.

The invention discloses application of alkalized tea saponin as a talc inhibitor, and belongs to the technical field of mineral separation. The molecular structural formula of the alkalized tea saponin is shown in the specification, wherein R is a sugar body unit; m is a cation; the alkalized tea saponin has a selective inhibition effect on flotation of talc, and is particularly suitable for flotation separation of metal sulfide ore (such as molybdenite, chalcopyrite, galena and sphalerite) and talc.

The invention relates to the technical field of biochemical wastewater treatment and automatic control, in particular to a comprehensive utilization and treatment system for dioscorea zingiberensis saponin wastewater. The data acquisition module comprises a data acquisition module, a prediction module, a quantification module, a risk hedging module and a control execution module; the system estimates key enzyme activity by adopting a non-linear model by collecting a water inlet fingerprint vector and a reactor state; the core of the method is that a system safety distance is determined according to enzyme activity and flora health degree, and control weights of three targets of collapse risk, effluent compliance and resource recovery are dynamically adjusted according to the system safety distance; when the safe distance changes, the system executes compound control through a multi-objective optimization algorithm; according to the method, prospective early warning of the core biochemical state is realized, and the defect of response lag of a traditional method is overcome.

The present invention belongs to the technical field of extraction and purification of fat-soluble chemical components, and particularly relates to a method for extracting and purifying dihydroquercetin from larch xylem. The present invention uses tea saponin aqueous micelles (tea saponin aqueous solution) to greenly extract dihydroquercetin from larch xylem, and then, according to the interfacial charge characteristics of the micelles formed by tea saponin and dihydroquercetin in the aqueous solution, utilizes the charge characteristics of chitosan to directionally enrich dihydroquercetin in the extract, and combines anti-solvent biphasic crystallization and water-solvent single-phase recrystallization to purify dihydroquercetin. The extraction and purification method provided by the present invention has a high extraction rate of dihydroquercetin and a high purity of the obtained pure product, and has the characteristics of simple operation, green safety, high efficiency and low cost. It not only improves the utilization rate of larch resources, but also has great significance for giving play to the high-value application of dihydroquercetin.

This invention relates to the field of natural product separation and recombination, and particularly to a recombinant saponin from *Gleditsia sinensis* and its preparation method. The method includes: 1) crude saponin extraction: water extraction of *Gleditsia sinensis* pericarp to obtain a crude saponin solution; 2) impurity removal: loading the crude saponin aqueous solution into macroporous adsorption resin AB-8 and eluting to remove impurities; 3) saponin component separation: eluting sequentially with 25-35% ethanol solution, 40-50% ethanol solution, 55-65% ethanol solution, and 70-80% ethanol solution to obtain S30, S45, S60, and S75 saponin components, respectively; 4) saponin hydrolysis: hydrolyzing the S45 saponin component to obtain hydrolyzed saponin; 5) saponin component recombination: recombining the saponin components obtained in steps 3) and 4) with the hydrolyzed saponin to obtain recombinant saponin from *Gleditsia sinensis*. This invention enables saponin purification and improves the surface activity of *Gleditsia sinensis*.

We provide novel, specific cytotoxic drug therapies with minimal side effects. [Solution] A composition for a membrane-permeable complex for transport across a lipid membrane is provided, comprising one or more molecular induction system (MGS) peptides and a cytotoxic agent. A method for treating cancer is also provided, comprising administering this composition to a cancer patient.

The invention discloses an environment-friendly coal mine dust falling agent and a preparation method thereof. The coal mine dust falling agent is prepared from peanut shell cellulose and a surfactant. The surfactant is one or more of tea saponin, sucrose ester, soapberry and lipopeptide. The preparation method of the coal mine dust reducing agent comprises the following steps: S1, extracting peanut shell cellulose from peanut shells by adopting an enzyme method; and S2, compounding the peanut shell cellulose and a surfactant to obtain the dust falling agent. Cellulose in peanut shells is extracted through an enzyme method and is compounded with surfactants such as biological tea saponin, sucrose ester, soapberry and lipopeptide, and the environment-friendly coal mine dust falling agent is prepared. The method has the advantages of being simple in process, easy to operate, low in raw material cost, biodegradable after spraying and dust falling, free of secondary pollution and good in application prospect in the field of coal mine dust falling.

Provided are variants of saponins that are found in Quillaja saponaria (QS) Molina tree bark that are chemically modified to distinguish them from naturally occurring parent saponins. The modified saponins have increased adjunctive activity compared to the unmodified parent saponins. Defined structures allow for comparisons of the modifying groups with respect to their respective adjunct activity and permit characterized vaccine formulations that have one or more defined saponins.

The invention relates to the technical field of aviation plant protection aids, and discloses an aviation plant protection aid containing natural plant polysaccharides and a preparation method of the aviation plant protection aid. Constructing a dynamic self-repairing system of a laccase catalysis phenol crosslinking component, a peroxidase catalysis polysaccharide coupling component and a cellulose degradation product repairing storage material, and a biodegradation regulation and control system integrating a cellulase-xylanase compound enzyme component, a tea saponin regulation component and a peptone nutrition component; the functions of enhancing leaf surface attachment, dynamic self-repairing and environment-friendly degradation of the auxiliary agent are realized through multiple synergistic effects. The technical problems that a traditional aviation plant protection auxiliary is insufficient in leaf surface adhesive force, damage cannot be automatically repaired, and environment residues are accumulated are solved, stable adhesion of liquid medicine under the severe weather condition, automatic repairing capacity after film damage and complete biodegradation within 30 days after the pesticide effect period is finished are achieved, and the effect is achieved. And the reliability, persistence and environmental friendliness of aviation plant protection are remarkably improved.

The present invention provides a kind of breeding method that improves the disease resistance of Pengze crucian carp myxosporeans, comprising the following steps: S1. breeding environment treatment, S2. purchasing healthy fry, S3. fry disinfection, S4. fry delivery, S5. feeding management, S6. water quality management, S7. disease prevention and detection, S8. emergency treatment. In the fry disinfection link, Pengze crucian carp fry are soaked by using a biological disinfectant composed of tea saponin, photosensitizing component, carvacrol, artesunate, and choline-lactic acid ionic liquid, and supplemented with LED light irradiation. These disinfecting components can work together after penetrating into the inside of the myxosporeans, inhibiting their growth and reproduction, thereby achieving the effect of effectively killing the myxosporeans. In the emergency treatment link, the insecticide composed of polysaccharide-mercaptosuccinic acid conjugate, antimicrobial peptide, artesunate, nano zinc oxide, triptolide, and carvacrol is used, and through the synergistic effect between the components, multi-target efficient killing of myxosporeans is achieved.

The invention discloses theasaponin E3, a separation and purification method thereof and application of the theasaponin E3 as an aurelia scyphistoma killing agent, and belongs to the technical field of natural pharmaceutical chemistry. The structure of the theasaponin E3 is shown in the specification, the theasaponin E3 is derived from plants, is a natural triterpenoid saponin compound, has stronger killing activity on aurelia scyphistoma compared with tea saponin, is single in component and clear in structure, and is beneficial to standardized application and potential ecological risk evaluation in the field of biological prevention and control of aurelia scyphistoma. # imgabs0 #

This invention relates to the field of natural material emulsification and discloses a natural material emulsification process for processing personal care products. The process includes: injecting a dispersed aqueous phase of tea saponin and soybean lecithin into the oil phase at a gradually decreasing pump flow rate at a temperature ranging from 30°C to 42°C, while simultaneously increasing the stirring speed; applying an ultrasonic pulse after the oil phase injection is complete; lowering the stirring speed to stabilize the interfacial film; and reducing the temperature in two stages. This invention matches the interfacial expansion rate with the molecular adsorption rate to eliminate localized oil phase overload, resulting in a uniform monodisperse state of the droplets. Simultaneously, it avoids the peeling and damage of the interfacial film by continuous strong shear fields, locks the lattice arrangement of hydrophobic hydrocarbon segments in the interfacial film, and forms a three-dimensional steric network, thereby increasing the interfacial yield stress, enhancing long-term thermodynamic stability, and suppressing stratification and phase coalescence during long-term storage.

Saponin extracts containing at least 88% QS-21 main peak and >3% to 10% 2018 component by UV absorbance at 214 nm, methods for making said extracts, their use as vaccine adjuvants and related aspects.

The invention relates to the technical field of natural product separation engineering, discloses a method for synchronously separating and purifying multiple active components of camellia oleifera shells based on synergistic extraction-gradient desorption-targeted refining, and aims to solve the technical bottleneck that high-purity tannin, proanthocyanidin and tea saponin cannot be efficiently and synchronously obtained from the camellia oleifera shells in the prior art. The core innovation is as follows: 1) an ultrasonic-microwave synergistic extraction parameter matching system is designed, and efficient synergistic dissolution of three active components is realized through precise matching of ultrasonic power and microwave power (0.5-1.7): 1 in combination with a 65-75% ethanol aqueous solution; (2) a three-stage incremental ethanol concentration gradient elution strategy is innovatively adopted, and directed preliminary separation of component groups is realized through step-by-step elution of 20-30% ethanol, 50-60% ethanol and 80-95% ethanol according to polarity differences of the three components; and (3) establishing a targeted refining technology combination, adopting Sephadex LH-20 gel chromatography according to the molecular weight characteristics of proanthocyanidin, and adopting high-speed counter-current chromatography of an ethyl acetate-n-butyl alcohol-water system in a specific ratio according to the structural characteristics of tea saponin homologues to realize deep purification. The continuous synchronous preparation of three active components in the camellia oleifera shells is realized for the first time, the purity of tannin in the obtained product is greater than or equal to 75%, the purity of proanthocyanidin is greater than or equal to 90%, and the purity of tea saponin is greater than or equal to 92% (the proportion of oleanane saponin is greater than or equal to 85%), the utilization rate of raw materials is increased by 30% or above, and the solvent consumption is reduced by 25%. An efficient, economical and environment-friendly integrated technical scheme is provided for high-value utilization of the camellia oleifera shells, and the method has a remarkable industrial application value.

The invention relates to a therapeutic combination, comprising a first proteinaceous molecule comprising a first binding site for binding to a first epitope of a first cell-surface molecule, the first proteinaceous molecule provided with at least one saponin covalently bound to an amino-acid residue of said first proteinaceous molecule, and comprising a second pharmaceutical composition comprising a second proteinaceous molecule different from the first proteinaceous molecule, the second proteinaceous molecule comprising a second binding site for binding to a second epitope of a second cell-surface molecule different from the first cell-surface molecule, and comprising an effector moiety, wherein the second epitope is different from the first epitope. An aspect of the invention is a composition comprising the first proteinaceous molecule and the second proteinaceous molecule of the invention. The invention also relates to a composition or therapeutic combination comprising an antibody—drug conjugate or antibody—oligonucleotide conjugate and the first proteinaceous molecule of the invention. An aspect of the invention relates to a pharmaceutical composition comprising the composition or the antibody—drug conjugate of the invention, and optionally further comprising a pharmaceutically acceptable excipient. The invention also relates to the therapeutic combination or the composition or the antibody—drug conjugate or the pharmaceutical composition of the invention, for use as a medicament. The invention also relates to the therapeutic combination of the invention for use in the treatment or prophylaxis of a cancer.