232 results about "Saporin" patented technology

The invention relates to a method for extracting camellia oil and tea saporin from tea seeds. The method provided by the invention comprises the following steps of: pre-treating tea seeds, extracting camellia oil in a water phase, extracting tea saporin, recycling a solvent and the like. The oil extracting rate obtained by using the method provided by the invention can be more than 95%, the recycling rate of the tea saporin is more than 85% and the oil quality of the camellia oil is good. In the method provided by the invention, ethanol or isopropanol is utilized as the solvent so that the camellia oil is safe and toxic, has a lower boiling point, is easily recycled and repeatedly utilized, can reduce manufacturing cost, is environmental-friendly and has a very wide application prospect.

The invention provides a green extraction technology for the comprehensive and efficient utilization of a Dioscorea zingiberensis resource. Saponins are extracted and simultaneously other effective components in Dioscorea zingiberensis, such as Dioscoreae starch, celluloses, a coronary heart disease control raw material, pectin, agricultural nucleic acids and the like, are comprehensively, efficiently and synchronously extracted through the technology. Rhamnose is extracted from a hydrolysis waste liquid; the whole process of the technology is green; and the technology has the advantages of great improvement of the saponin production level in China, improvement of the resource utilization efficiency, energy saving, environmental protection, realization of the unification of the economic benefit, the environmental benefit and the social benefit, and promotion of the sustainable development of Chinese economy and society.

The present invention relates to a method for environment-protecting pollutionless production of diosgenin. Before the steps of extraction, concentration and crystallization of conventional method the steps of raw material pretreatment, sieving, membrane filtering and dewatering, fermentation, hydrolysis, filtration, secondary hydrolysis and water-washing are successively implemented or the steps of raw material pretreatment, sieving, membrane filtering and dewatering, adding solvent to make extraction separation to twice, combining extracts, distilling, fermentation and hydrolysis are successively implemented. It has no need of complex special equipment, and can purify and produce diosgenin as basic raw material of steroid hormone medicine by utilizing diascorea plant.

The present invention provides a method for preparing tea-saponin pond-cleaning agent by using tea dregs as raw material. Said method includes the following steps: adopting hydrophilic solvent aqueous solution to extract dried tea dregs for three times at 50-75 deg.C, combining extracts, filtering said extract mixture, making the filtrate undergo the processes of decompression recovering solvent and concentration to obtain concentrate, adding soluble starch, edible salt and calcium hydroxide, spray-drying so as to obtain the invented tea saponin pond-cleaning agent.

The key of this invention relates to a method to extract saponin after separating starch and fiber from ginger, which includes the following steps to achieve: 1) initial processing of raw materials: impurities removal of ginger, high pressure jet wash, first class coarse powder, second class refined powder, make the serous size reach 30-80 mesh and concentration 14-18%, 2) separation of starch and fiber: starch counter current scrubber isolated starch and fiber from the serous fluid, to get a mixture, 3) precipitation to remove water: heating the mixture to 32-40deg.C, naturally precipitating for 6-8 hour and concentrating the supernatant to the concentration of 15-20% with ultrafiltration membrane, 4) hydrolysis drying: hydrolysis of the mixture, deacidification, dehydration, and drying, 5) saponin extraction: 120 # gasoline is used for extraction, concentration, crystallization, bleaching and drying.

The biological technological process of producing citric acid and saponin with yam plant material includes the biological technological process of producing citric acid with yam plant material and the biological technological process of producing saponin with the yam plant material residue. The present invention has the advantages of raised citric acid production utility and reduced environmental pollution caused by saponin production. The present invention has greatly raised saponin yield and quality, citric acid converting rate as high as 35 % and yield of 85 % and saponin yield of 0.6-0.8 %.

The invention relates to a waste water treating technique to extract yam saponin by ginger or Rhizoma Dioscoreae Nipponicae. It is a method to treat yam saponin waste water by film integration technique and recover glucose and HCl, including the steps: 1) pretreating: prefiltering the yam soaponin waste water to eliminate suspended substance or macromolecular impurities and obtain filtrate; 2) recovering glucose and HCl: treating the filtrate by acidproof polyamide nano-filter membrane, where the HCl solution permeates the nano-filter membrane at 1.6-2.5 MPa and 20-40 deg.C to obtain HCl, but the glucose is withheld; the withheld glucose solution's concentration varies from 2-7% to above 10% by condensation, when the concentrated solution reaches below 40% of the charging capacity, it starts supplying water to the concentrated solution to eliminate the remanent acid, and keeps vaporizing and concentrating to make the glucose; 3) deep treating: the penetrant is neutralized to achieve the standard and be discharged.

The invention discloses siraitia grosvenorii water-retaining bactericidal coating liquid. The siraitia grosvenorii water-retaining bactericidal coating liquid consists of the following components in percentage by mass: 15-20 percent of lac, 0.1-0.2 percent of monoglyceride, 0.3-0.5 percent of sodium carbonate, 0.001-0.004 percent of tea saponin, 0.002-0.004 percent of myristic acid and 0.001-0.002 percent of aminooxy acetic acid. According to the coating liquid, the freshness retaining cost can be reduced, cold-resistant pathogenic bacteria are not generated, the breathing of siraitia grosvenorii and the growth of surface pathogenic bacteria can be inhibited, and thus the rot of the siraitia grosvenorii is avoided. The water-retaining bactericidal coating liquid is high in stability and can be stored for half a year without deterioration. According to the water-retaining bactericidal coating liquid disclosed by the invention, the surface of the siraitia grosvenorii can be bright, and after the siraitia grosvenorii is stored for 100 days, the bad fruit rate is less than 2 percent, the water loss rate is less than 10 percent, and the taste is kept fresh.

The invention relates to a natural air freshener which mainly consists of natural bacteriostatic active substances and a natural emulsifier and has a sterilizing function. The air freshener is prepared from the following components in percentage by weight: 1-3% of zanthoxylum oil, 0.5-2% of clove essential oil, 0.5-2% of litsea cubeba oil, 0.5-1% of oregano essential oil, 0.5-1% of usnic acid, 0.01-0.2% of tremella polysaccharide, 3-5% of one or more of tea saponin or soapberry saponin or saponin and 85.80-93.99% of de-ionized water. By emulsifying and dispersing essential oil ingredients by virtue of the natural emulsifier, the natural air freshener disclosed by the invention, different from conventional alcohol solvent products, is safe and not stimulating; meanwhile, with the significant bacteriostatic activity, the natural air freshener can be used for effectively inhibiting the growth of planktonic microorganisms in public places (railway station, movie theater and the like), hotels, rooms, cars and the like, and can be used for freshening the air and removing odor.

Inhibitors of saporin-L1 are disclosed, as are related compositions and uses thereof, in particular in cancer therapy that employs saporin-L1-linked immunotoxins.

The invention belongs to the technical field of natural product detection, and relates to a quantitative detection method of UPLC-PDA-MS/MS of triterpenoid saponins in Camellia sinensis (L.) O.Kuntze.The UPLC-UV-QTOF-MS/MS method of quantitative determination of the triterpenoid saponins in the Camellia sinensis (L.) O.Kuntze comprises the following steps that (1) samples to be detected are pretreated; (2) any one or more saponin compounds known in the Camellia sinensis (L.) O.Kuntze are taken, and a standard product solution is prepared; and (3) the structure of high performance liquid chromatography/ultraviolet/mass spectrometry combination equipment (UPLC-PDA-QTOF-MS/MS) of a test solution and a standardized product solution is identified by tandem mass spectrometry, and an external standard method is used for quantitative detection under a PDA detector. According to the UPLC-UV-QTF-MS/MS method of quantitative detection of the triterpenoid saponins in the Camellia sinensis (L.) O.Kuntze, quantitative results are more accurate; the sensitivity is high; and the sample demand amount is less.

This invention relates to a method to efficiently extract diosgenin from the root and stem of dioscorea zingiberensis without fermentation, that is, a proper amount of water is added and the root and stem of dioscorea zingiberensis are pulverized. Fiber sediments and starch in the sludge are separated by a mechanical method and the pH value of the sludge is immediately adjusted to below 4.5 with acid. The adjusted and unfermented sludge is concentrated and acid hydrolyzed with the aid of hydrolysis agent. The hydrolyzed mixture is filtered to remove acid, neutralized, dried, extracted with organic solvent and crystallized so as to obtain diosgenin product.as fermentation is purposely inhibited and avoided during acid hydrolysis of the concentrated sludge in this invention, not only the stench problem is solved, but also the hydrolysis effect is greatly promoted. The extracted diosgenin product is of good crystallinity, high purity, white color and its melting point can be above 200 deg. C.

The invention discloses an antimicrobial preparation used in female period and before and after sexual life for health care and a preparation method thereof, and belongs to the technical field of pharmaceutical health care. The preparation consists of raw materials with the following weight percentage composition prepared: 1-10 parts of tea saponin, 1-5 parts of organic zinc, 10-20 parts of alkyl polyglucoside, 0.2-5 parts of xanthan gum, 0.1-5 parts of citral, 0.1-5 parts of cinnaldehyde, 0.1-10 parts of vanillin, 2-15 parts of lactic acid, 1-5 parts of caprylic capric triglycerride, 1-10 parts of glycerol, 0.1-3 parts of menthol and a proper amount of deionized water. The preparation employs compound formula of natural plant extracts and food additive organic zinc and prepares the acidic antimicrobial agent with functions of cleaning, antibiosis, health care and nursing through modern nano technology.

The invention provides an extraction process of diosgenin, and particularly discloses a process for extracting saponin in yam through microbial strengthened fermentation in combination with a dilute acid catalytic hydrolysis method. The process comprises the following steps of: cleaning and crushing the yam; performing the microbial strengthened fermentation to separate the saponin from starch and cellulose; then hydrolyzing with dilute acid under the action of a catalyst to obtain the diosgenin; performing soxhlet extraction on hydrolysate by using a solvent; and finally crystallizing and drying to obtain the finished product diosgenin. The process has the advantages of simple process, short process time, less acid consumption, less wastewater, reduced COD (Chemical Oxygen Demand) in the wastewater, extreme easiness in treatment and capability of effectively reducing the cost and the pollution.

The invention discloses a method for decoloring crude tea saporin, which comprises the steps of adding a compound decolorant into a solution of crude tea saporin and reacting for 2 minutes at room temperature with stirring, wherein the compound decolorant is prepared by mixing metal complex hydride and acid accumulator sulfite in a mass ratio of 3:1-10:1, and the adding amount of the compound decolorant is 1 to 5 percent of the mass of solute in the solution of crude tea saporin. The decolorant is only reacted with polyphenol and flavone pigment components in the crude tea saporin to realize the decoloring function; and the surface activity of the decolorized tea saporin such as wetting, emulsification, foaming and foam stability is not influenced.

This invention provides a new technology of cleaning production that extract sapogenin from dioscorea zingiberensis wright. It uses rhizome of fresh dioscorea zingiberensis or fresh dioscorea nipponica that are 2 -3 perennials as raw material. Its technology is that: raw material-microwave processing-ultramicro-pulverization-dynamic state continuous extracting-rectification-hydrolization-crude sapogenin-dissolving adsorption for purification-rectification-crystallization-drying-depurating sapogenin. This invention provides a new technology that produces diosgenin. Its technology is advanced, investment is little, material expenditure is low, production yield is high, quality is satisfactory, production period is so short that extracting technology can complete in 20 hours. It has no pollution. Content of COD in the water is less than 300mg/l.

The invention relates to a natural environment-friendly washing powder and a preparation method thereof, belonging to the technical field of washing. The washing powder mainly comprises the following raw materials in percentages by weight: 40 to 60% of yellow soybean, 20 to 30% of pea and 20 to 30% of green soybean. The method comprises the following steps of: removing impurities, air drying, grinding into fine powder of 100 to 180 meshes, sealing and packaging. Saponin foams which the washing powder is rich in can remove grease and dirty. The washing powder is safe and environment-friendly and is beneficial to people health.

The invention discloses a hydrogen production chlamydomonas chloroplast separation method which comprises the following steps: 1) hanging Chlamydomonas in a lysate, adding saponin until the final concentration is 0.25g / 100mL; 2) putting the step 1) solution on the ice for 1-2min for chlamydomonas cell lysis; 3) centrifuging the step 2) solution for 1min at 2300g at 4 DEG C; 4) using the lysate for rinsing steps 3) precipitate, centrifuging for 1min at 1000-2300g at 4 DEG C to obtain crude chloroplast extract; 5) hanging the crude chloroplast extract in a lysate; 6) adding 75% by volume of Percoll on the centrifugal pipe bottom, adding 45% by volume of Percoll into the upper layer, adding the step 5) solution into the uppermost layer to the form the gradient, centrifuging for 20min at 4600g at 4 DEG C to form dark green bands between two different volume fractions of Percoll; and 7) taking the dark green bands out, using the lysate to dilute, centrifuging for 2min at 890-1000g at 4 DEG C, and collecting precipitate to obtain chlamydomonas chloroplast. The experiment proves that the method has the advantages of simple operation, high chlamydomonas chloroplast yield and shorter time.

The invention discloses a method for extracting high-purity tea saporin by taking water as a solvent. The method is characterized by comprising the following steps of: uniformly smashing a tea seed cake to 1-3 millimeters; frying a blank to 138-141 DEG C; curing impurities in the blank; reversely soaking by taking water as a lytic agent for 5 times; stirring in a soaking pond at the speed of 30 revolutions per minute till the concentration of the stirred leachate is up to 8-10 baume and the solid content is 25-27 percent; and filtering, concentrating and drying to obtain high-purity tea saporin. The method has the advantages of simple process, low production cost and capability of extracting the high-purity tea saporin since the water is taken as a soaking liquid.

The invention discloses a quantitative detection method for saponin in tea saponin. According to the quantitative detection method, 3.0g-3.5g of extract of tea seed or the tea saponin is taken, the extract is subjected to alkali and acid hydrolysis and then is dissolved in water, hydrolysate is poured into the water to allow precipitation of the extract so as to measure mass of sapogenin, and then the mass of the sapogenin is converted to mass fraction of the saponin. The quantitative detection method has the characteristics that equipment is simple, the cost is low, the operation is convenient and easy, the accuracy is high, and the direct detection can be preformed, thus being suitable for detection on tea seeds, crude extract of the tea saponin, and high-purity tea saponin. The quantitative detection method is particularly applicable to pioneering small and medium-sized enterprises which are lack of instruments and equipment and are equipped with personnel with low education degree.

The invention discloses a medicament composition for preventing and treating grassland spodoptera litura. The medicament composition comprises auxiliary materials, an effective component A and an effective component B, wherein the effective component A is tea saponin, the effective component B is chlorantraniliprole or tolfenpyrad, and the mass ratio of the effective component A to the effective component B is (1-20): (20-1). The tea saponin and the chlorantraniliprole or tolfenpyrad are mixed to prepare an insecticide with a certain proportion, the insecticide is used for preventing and treating the grassland spodoptera litura, the effect is good, the dosage is low, and the resistance of the grassland spodoptera litura to the insecticide can be well delayed.

Provided is a method for screening microorganism strains for efficient degradation of tea seedcake meal. The method enables the microorganism strains to be obtained by sequentially performing a primary screening of a high-concentration tea seedcake meal culture medium, a secondary screening of a casein culture medium, a third-time screening of a carboxymethyl cellulose culture medium and bottle shaking fermentation secondary screening. The strains have tolerance to high-concentration tea saponin of the tea seedcake meal, high protease activity and performance of activity of high cellulose enzyme simultaneously. The strain-fermented tea seedcake meal obtained by the method has nutritional value higher than that the tea seedcake meal which is not fermented, protein content of crude protein of a product is 8%-12%, free amino acid content is over 7.5% and is improved by 40%, crude fiber content is 20%-25%, reducing sugar content is over 6.0% and is improved by 15%, saponin content is below 0.5%, and detoxification rate is over 95%. The method well solves the problems of toxicity of the tea seedcake meal and low utilization rate of the crude fiber and the crude protein in the prior art. A production process method of the multi-strain fermented tea seedcake meal can be simplified by utilizing the method to obtain the strains, and the method can obtain the microorganism strains which are simplified in process and good in degradation effects.

The invention provides a method for cleanly producing saponin by synthetically using yellow ginger, which can be realized by the following technique steps: (1) the yellow ginger is cleaned and ground to pulp after being crashed, particles in the pulp are led to reach 18 to 40 mashes, and mass percentage density is 10 percent to 15 percent; (2) two horizontal spiral unload settling centrifugal machines are used for the separation of the second level, and the saponin, fibers and starch are separated to obtain saponin pulp; (3) at the temperature of 34 DEG C to 38 DEG C, the saponin pulp is fermented naturally for 24 hours to 36 hours; (4) the fermented saponin pulp is filtered repeatedly by an ultra-filtration membrane and a disk plate to obtain the condensed saponin pulp with the mass percentage density of 15 percent to 20 percent and a permeate liquid; (5) finished products can be obtained by acid hydrolyzing, extracting, condensing and crystallizing; and (6) proteins in the permeate liquid obtained in step (4) are recycled, and the remained permeate liquid is sent to a sewage disposal system; the wastewater in the acid hydrolysis is also sent to the sewage disposal system after neutralization reaction, and after being treated, the two are used for cleaning the yellow ginger.