Method for screening microorganism strains for efficient degradation of tea seedcake meal

A tea seed cake and bacterial strain technology, which is applied in the field of screening biological strains for efficiently degrading tea seed cake, can solve problems such as the lack of systematic research methods for degrading tea seed cake, and achieve easy scale production, small investment, and high degradation good effect

Inactive Publication Date: 2012-06-27
林元山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these studies solve one of the problems from one aspect of tea seed cake, either only the research on the detoxification of tea seed cake, or the research on the degradation of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020]Example 1: Screen high-concentration tea saponin-resistant strains, prepare high-concentration tea seed cake solid medium, wherein tea seed cake 10%, agar 2.0%, water 88.0%, constant volume 100 mL, pH natural, Sterilize at 121°C for 25 minutes, pour 8 plates, apply 0.1 mL of the soil sample dilution solution to each plate according to the 10-fold dilution method, and incubate at 35°C for 4 days to obtain large colonies, good expansion, and tea saponin resistance. Seven recipient strains, numbered lys2201, lys2202, lys2203, lys2204, lys2205, lys2206, lys2207, were transferred to the slant.

Embodiment 2

[0021] Example 2: Screen high protease activity bacterial strains, prepare casein solid medium, wherein casein 2.0%, after heating and dissolving with 0.1 mol / L dilute hydrochloric acid, glucose 3.0%, agar 2.0%, adjust with 0.1 mol / L sodium hydroxide pH to 5.5, dilute to 100 mL with tap water, sterilize at 121°C for 25 min, pour 8 plates. Inoculate the strains lys2201, lys2202, lys2203, lys2204, lys2205, lys2206, and lys2207 on the plate of casein medium, culture them at 33°C for 2.5 days, and transfer the strains with a large ratio of hydrolysis zone diameter to colony diameter and good expansion Connect to a sterile slant, in which the ratio of the hydrolysis circle diameter to the colony diameter of strains lys2201, lys2203, lys2204, lys2205, and lys2207 is greater than 5.0, and transfer to a slant for further re-screening.

Embodiment 3

[0022] Example 3: Screen high cellulase activity bacterial strains, prepare carboxymethyl cellulose solid medium, wherein carboxymethyl cellulose 2.0%, after tap water is heated and dissolved, peptone 0.5%, agar 2.0%, natural pH, set with tap water Make up to 100 mL, sterilize at 121°C for 25 min, pour 8 plates. Inoculate the strains lys2201, lys2202, lys2203, lys2204, lys2205, lys2206, and lys2207 on a carboxymethylcellulose medium plate, culture at 33°C for 3 days, and stain the plate with 5 mL of 0.2% sterile Congo red liquid. min, and then decolorized with 2.0% sterile sodium chloride solution for 5 min, and selected strains with a large ratio of hydrolysis circle diameter to colony diameter and good growth potential were transferred to sterile slant again. The ratio of ring diameter to colony diameter is greater than 4.0, and transferred to a slope, stored in a 4°C refrigerator for later use.

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Abstract

Provided is a method for screening microorganism strains for efficient degradation of tea seedcake meal. The method enables the microorganism strains to be obtained by sequentially performing a primary screening of a high-concentration tea seedcake meal culture medium, a secondary screening of a casein culture medium, a third-time screening of a carboxymethyl cellulose culture medium and bottle shaking fermentation secondary screening. The strains have tolerance to high-concentration tea saponin of the tea seedcake meal, high protease activity and performance of activity of high cellulose enzyme simultaneously. The strain-fermented tea seedcake meal obtained by the method has nutritional value higher than that the tea seedcake meal which is not fermented, protein content of crude protein of a product is 8%-12%, free amino acid content is over 7.5% and is improved by 40%, crude fiber content is 20%-25%, reducing sugar content is over 6.0% and is improved by 15%, saponin content is below 0.5%, and detoxification rate is over 95%. The method well solves the problems of toxicity of the tea seedcake meal and low utilization rate of the crude fiber and the crude protein in the prior art. A production process method of the multi-strain fermented tea seedcake meal can be simplified by utilizing the method to obtain the strains, and the method can obtain the microorganism strains which are simplified in process and good in degradation effects.

Description

technical field [0001] The invention relates to a set of methods for screening biological strains for efficiently degrading tea seed cake, and belongs to the biotechnology field of fermentation engineering and biomass resource reuse. Background technique [0002] Camellia oleifera is an important oil crop in the southern hilly areas of my country, accounting for more than 80% of the national woody edible oil crops. Tea seed cake, also known as tea meal, also known as tea bran, tea dry, tea seed cake. It is purple-brown granule, which is the residue left after the wild camellia oil fruit is pressed. The cake after tea seed oil extraction also contains a large amount of starch, protein, crude fat, soluble sugar and tea saponin, etc., which can be extracted and utilized again. The area of ​​camellia oleifera forest in my country is about 4 million hectares, and it is the country with the most varieties, the widest distribution and the largest output of camellia oleifera in th...

Claims

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Application Information

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IPC IPC(8): C12Q1/37C12Q1/34C12Q1/04C12N1/00A23K1/14
Inventor 林元山刘来壮吴永尧
Owner 林元山
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