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Gardenia alkanivorans YC-RL2 and application thereof

A YC-RL2, Alkali Gordonia technology, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problem of low dissolved oxygen, achieve good economic value and application prospects Effect

Active Publication Date: 2015-12-30
中国农业科学院研究生院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few studies on the biodegradation of pollutants in industrial wastewater by microorganisms, mainly due to the harsh conditions of industrial wastewater, such as high salinity, extreme pH, low dissolved oxygen, etc.

Method used

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  • Gardenia alkanivorans YC-RL2 and application thereof
  • Gardenia alkanivorans YC-RL2 and application thereof
  • Gardenia alkanivorans YC-RL2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and Identification of Gordonia Alkalivora YC-RL2

[0033] 1. Isolation of strains

[0034] Activated sludge samples were collected from oil-contaminated farmland soil in Dongming County, Heze City, Shandong Province. Under aseptic conditions, 5g of activated sludge samples were inoculated into 50mL of inorganic salt ion medium containing 100mg / LDEHP, and cultured at 30°C and 180rpm. After every 7 days of culture, 1 mL was transferred to fresh inorganic salt medium, and transferred 3 times in a row.

[0035]Streak the acclimatized bacterial solution onto an inorganic salt medium plate containing 100mg / LDEHP, and culture in a 30°C incubator for 3 days. Pick a single colony on the plate and transfer it to inorganic salt culture with a concentration of 100mg / LDEHP for 7 days. Repeat three times until a purified strain is isolated, and the strain is named YC-RL2.

[0036] 2. Morphological characteristics of the strain

[0037] The bacterium is Gram-p...

Embodiment 2

[0044] Degradation performance test of embodiment 2 Gordonella alcaliphagus YC-RL2

[0045] 1. Gordonia alcaliphari YC-RL2 di(2-ethylhexyl) phthalate (DEHP), dicyclohexyl phthalate (DCHP), dimethyl phthalate ( DMP), di-n-butyl phthalate (DBP), diethyl phthalate (DEP) degradation

[0046] Gas chromatography (HPLC) was used to detect the degradation of Gordonia alcalivora YC-RL2 on DEHP, DCHP, DMP, DEP and DBP in inorganic salt medium, and the concentration tolerance to DEHP.

[0047] Inoculate strain YC-RL2 into liquid LB medium for activation, and cultivate to logarithmic growth phase OD 600 =0.8, according to the volume ratio of 10% inoculum was inoculated into the inorganic salt medium containing DEHP, DCHP, DMP, DEP and DBP respectively 100mg / L, as the treatment group, with the non-inoculated strain containing DEHP, DCHP, DMP , DEP and DBP each 100mg / L mixture of inorganic salt medium as a control group, the control group and the treatment group were respectively set up t...

Embodiment 3

[0068] Example 3 Application of Alkaliphagous Gordonella YC-RL2 in Bioremediation of Contaminated Soil

[0069] The soil used in this example was obtained from the Ximen Garden of the Chinese Academy of Agricultural Sciences. The Alkalivora Gordonia YC-RL2 was cultured in LB liquid medium to the logarithmic phase (OD600 =0.8, the cell concentration is about 2×10 8 CFU / mL), adding the prepared bacterial solution to the soil to a final concentration of 1×10 4 , 5×10 4 , 1×10 5 , 5×10 5 , 1×10 6 and 5×10 6 CFU / g soil (100g soil for each treatment), and add DEHP, DCHP, DMP, DEP and DBP to the soil respectively to each concentration of 100mg / kg, as a treatment group; meanwhile, add the same concentration under the same conditions Pollutant and non-inoculated soil was used as the control group, and the finally obtained culture system (treatment group and control group) was fully mixed. The samples were cultured in a constant temperature and humidity incubator at 30° C. with a...

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Abstract

The invention provides gardenia alkanivorans YC-RL2 capable of degrading multiple phthalic acid ester substances. The preservation number of the gardenia alkanivorans YC-RL2 is CGMCC No.10992. The gardenia alkanivorans YC-RL2 can 100% degrade 100 mg / L phthalic acid (2-ethyl caproyl) ester (DEHP) included in an inorganic salt culture medium in five days and meanwhile can further degrade DCHP, DMP, DBP and DEP. The gardenia alkanivorans YC-RL2 can be applied to degradation of phthalic acid ester pollutants, has good application potential for bioremediation treatment for environmental pollution caused by the pollutants, and has high economic value and application prospects.

Description

technical field [0001] The present invention relates to the field of microbiology and biodegradation, in particular, relates to Alkaligordonia YC-RL2 and its application. Background technique [0002] PhthalateAcidEsters (PAEs for short) is an important class of industrial raw materials, widely used in plastics, pesticides, cosmetics, paints, rubber and other industries, it has carcinogenic, mutagenic and reproductive developmental toxicity, etc. Toxicity can enter the environment through many ways, and the environmental problems caused by it have attracted worldwide attention. Due to the extensive use of phthalates by humans, they enter the atmosphere, soil, and water environment through different channels, causing varying degrees of pollution to the biosphere. PAEs are closely related to our daily life and can enter the human body through breathing, eating, drinking and skin contact, causing varying degrees of harm to human health. The common ones are bis(2-ethylhexyl) p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/34
Inventor 闫艳春任磊贾阳乔铖
Owner 中国农业科学院研究生院
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