28 results about "Exocellulase activity" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

The application discloses a high-efficiency lignocellulose-degrading filamentous fungus mutant strain and application thereof. The high-efficiency lignocellulose-degrading filamentous fungus mutant strain is formed by knocking out a wild-type filamentous fungus Podospora anserina photoperiod gene PaFrq. The application firstly finds that the filamentous fungus Podospora anserina with the knocked-out photoperiod gene PaFrq has the advantages of delayed aging, prolonged life cycle, improved growth speed, higher efficiency in degrading lignocellulose, especially improved cellulase activity in dark conditions, higher efficiency in degrading lignocellulose, and important significance for a fungus fermentation industry, especially for more efficient conversion of fungal biomass.

The application discloses a method for improving cellulase activity by using a combination of an endogenous promoter and an associated 5' UTR of Trichoderma reesei and an engineering strain constructed by the method. TrAE The 5' UTR upstream of the TrAE gene TrAE Overexpressing a transcriptional regulator xyr1 The expression cassette of the gene is transformed into Trichoderma reesei Δ ku70 Rut‑C30 The application also discloses a high-yield cellulase Trichoderma reesei strain QEaeuX, and a method for producing high-activity cellulase by using the strain. ku70 Rut‑C30 The cellulase activity produced by the strain in a 96 h shake flask culture is 2.6 IU / mL, which is 101% higher than that of the original strain Δ The application has a good industrial development and application prospect.

This solution discloses a high-temperature fermentation strain for Maotai-flavor baijiu brewing and its application in the field of functional microbial technology. The strain was isolated from mature Maotai-flavor baijiu mother koji and identified as *Bacillus cereus* XJ143 by 16S rRNA sequencing, possessing high cellulase activity. This strain is heat-resistant and suitable for the Maotai-flavor step-by-step koji-making process, solving industry problems such as insufficient decomposition of raw materials in traditional koji, insufficient pyrazine roasting aroma, and large batch-to-batch quality fluctuations in naturally produced koji. It can directly prepare liquid seed culture or solid inoculum for mother koji expansion, high-temperature koji, and mash fermentation, exhibiting strong adaptability for industrial production. *Cropensis ivory* XJ112 possesses high protease and amylase activity. The combination of *Bacillus cereus* XJ143 and *Cropensis ivory* XJ112 combines the secretion abilities of protease, amylase, and cellulose, efficiently breaking down wheat cell walls and simultaneously decomposing raw starch and protein.

The application discloses a kind of forestry waste treatment methods for improving forest carbon sequestration capacity, belong to forestry waste processing technical field, the method is to carry out composting after forestry waste is returned to forest, specifically including material crushing, carbon-nitrogen ratio adjustment, moisture content and pH adjustment, heap preparation and maturity and the like step, by preparing enzyme activity control agent and mixing into material slag to compost, the high concentration glucose produced in high temperature stage decomposition is absorbed by the prepared enzyme activity control agent, to reduce the inhibitory effect of high concentration glucose on cellulase, while the control agent further improves cellulase activity, further control to water, pH and the like condition, guarantee composting efficiently, improve composting rate and degree of maturity, the fertilizer obtained by composting can effectively improve the physicochemical properties of soil in forest, promote the growth of forest trees, improve stand quality and carbon sequestration capacity, the forestry waste treatment method of the application has important significance to the resource utilization of forestry waste and forest carbon sequestration and sink.

The invention provides a method for relieving inhibition of phenolic compounds on cellulase in a wood fiber saccharification system. The method is used for inhibiting covalent cross-linking of phenolic compounds and cellulase, and comprises one or more of reduction of the content of the phenolic compounds in a system, enzymolysis saccharification under an anaerobic condition and addition of an inhibitor for competitive binding of the phenolic compounds. The method for reducing the content of the phenolic compounds specifically comprises the following steps: carrying out alkaline pretreatment on a wood fiber raw material and / or carrying out water extraction treatment on the wood fiber raw material / a pretreated solid sample. The inhibitor is an amino-containing compound, and the amino-containing compound is ethylamine, L-lysine or cysteine. The method is a technical scheme provided based on a new discovery of an inventor on a mechanism of inhibiting cellulase activity by phenolic compounds in a lignocellulose saccharification system, the inhibition of the phenolic compounds on cellulase in the saccharification step is fundamentally relieved, and the saccharification efficiency of lignocellulose is remarkably improved. The invention also provides a method for remarkably improving the saccharification efficiency of the wood fibers, and the method inhibits covalent crosslinking of the phenolic compounds and the cellulase, so that the saccharification efficiency is remarkably improved, and the method has important industrial application value.

The invention provides a Trichoderma reesei engineering bacterium for high yield of beta-glucosidase, which is characterized in that a Trichoderma reesei SN1 strain is used as a starting strain, and an expression cassette for knocking out a cre1 gene, a constitutive expression cassette of an aspergillus niger source bglA gene expressed by a promoter cbh1 and a constitutive expression cassette of a 130442 gene expressed by the promoter cbh1 are constructed in a genome of the Trichoderma reesei SN1 strain. Through the knockout of the cre1 gene, the overexpression of the aspergillus niger source bglA gene and the overexpression of the 130442 gene, the yield of the beta-glucosidase in the trichoderma reesei is improved synergistically. Glucose is taken as a raw material, a transglycosylation reaction can be catalyzed by directly utilizing a crude enzyme solution of trichoderma reesei engineering bacteria with high yield of beta-glucosidase to prepare beta-disaccharide, and the beta-disaccharide can be taken as an inducer for cellulase production; a crude enzyme liquid with higher cellulase activity and beta-glucosidase activity is obtained by supplementing an inducer and carrying out fed-batch fermentation.

The invention relates to the technical field of new strains for degrading cellulose, in particular to a pseudoxanthomonas strain JC1303 and application thereof. Through whole genome sequencing and function annotation, it is systematically revealed for the first time that the strain carries a complete cellulose degrading enzyme system including incision beta-1, 4-glucanase, cellulase and beta-glucosidase, has a plurality of central metabolic pathways supporting efficient degradation and utilization of cellulose, and can be used for degrading cellulose. And specific gene resources are determined through generic genome analysis. The strain has the remarkable technical effects that the strain shows higher cellulase activity after being cultured for 5 days, and cellulose materials such as agricultural wastes and the like can be stably and efficiently degraded in a salt-containing environment by virtue of the unique genetic background and marine source characteristics of the strain.

The invention discloses a fermentation engineering bacterium Lactobacillus plantarum JY003 for wine-processed traditional Chinese medicine decoction pieces, the preservation number of the fermentation engineering bacterium is CCTCC (China Center For Type Culture Collection) NO: M 20252157, the preservation date is September 29, 2025, and the classification name of the fermentation engineering bacterium is Lactobacillus plantarum JY003. The preserved strain can tolerate alcoholic strength of 10-15%, has both pectinase activity and cellulase activity, can keep high cellulase activity and pectinase activity under the yellow wine processing condition, further promotes plant cell wall degradation, enables active ingredients in cells to be released and dissolved out, can be used as a wine-processed traditional Chinese medicine decoction piece fermentation engineering strain, and has a wide application prospect. The method is used for preparing wine-processed traditional Chinese medicine decoction pieces.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

The application discloses a shrimp intestinal tract Tenacibaculum lutimaris strain and application of the shrimp intestinal tract Tenacibaculum lutimaris strain in shrimp culture, and has the characteristics that the strain is BG12 strain, is classified and named as Tenacibaculum lutimaris, is preserved in the China General Microbiological Culture Collection Center on July 8, 2022, and has a preservation number of CGMCC No. 25267, the application of the shrimp intestinal tract Tenacibaculum lutimaris strain in preparation of a Vibrio parahaemolyticus inhibitor and in preparation of a probiotic additive for shrimp culture, and the advantages are that the shrimp intestinal tract Tenacibaculum lutimaris strain has amylase and cellulase activities, and can improve the survival rate of shrimp culture and the anti-invasion ability of shrimp culture to Vibrio parahaemolyticus.

The invention relates to the field of microorganisms, in particular to bacillus subtilis MG-D587 for high-yield digestive enzyme and application of the bacillus subtilis MG-D587. The preservation number of the bacillus subtilis MG-D587 is CGMCC (China General Microbiological Culture Collection Center) No.34354, the preservation time of the bacillus subtilis MG-D587 is 2025-4-25, and the bacillus subtilis MG-D587 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The bacillus subtilis MG-D587 is separated from a healthy human body and has the capacity of producing protease, amylase, lipase and cellulase, the activity of the amylase and the activity of the cellulase are remarkably higher than those of positive strains, and it is indicated that the bacillus subtilis MG-D587 has the huge potential of promoting food digestion and absorption and improving intestinal health.

The invention relates to the technical field of wood protective agents, and discloses ammonium bis (oxalato) borate and a preparation method and application thereof in wood protection, and the preparation method of the ammonium bis (oxalato) borate comprises the following steps: oxalic acid, boric acid and water are mixed and stirred to obtain a mixed solution; and mixing the mixed solution with an ammonium compound, and carrying out heating reaction to obtain ammonium bis (oxalate) borate. The ammonium bis (oxalato) borate prepared by the method has a remarkable nitrogen-boron synergistic flame retardant effect, the flame retardant property of wood treated by the ammonium bis (oxalato) borate can reach the B1-level flame retardant standard, and the heat release and smoke generation amount are remarkably reduced; wherein the boron element can destroy the enzyme system of wood-rotting fungi (inhibit the activity of cellulase) and interfere the digestive system of termites (destroy the intestinal flora) at the same time, so that the environment-friendly and low-toxicity characteristics of the traditional boron system are reserved, and the effects of corrosion prevention and insect prevention are achieved. In addition, the preparation process provided by the invention is green, environment-friendly, low in cost and easy for large-scale popularization and application.

The present application relates to the field of microorganisms, and provides a propionibacterium acidifaciens BL8 with functions of reducing blood sugar and resisting aging, and application thereof, the propionibacterium acidifaciens BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No.33134.The propionibacterium acidifaciens BL8 of the present application can inhibit DPP4 activity, promote INS-1 insulin secretion, inhibit acetylcholinesterase activity and superior cellulase activity.Can reduce blood sugar;Improve memory, prevent and relieve Alzheimer's disease;Promote cellulose absorption, improve constipation, and have high research and application value in reducing blood sugar and resisting aging.

The invention discloses bacillus, a microbial inoculum and application of the microbial inoculum in fermentation of broccoli or broccoli rotten vegetable, and relates to the technical field of agricultural waste recycling. The bacillus sp. Is screened and separated from cow dung subjected to high-temperature composting, can grow under a high-temperature condition, and has remarkable protease and amylase activity and weak cellulase activity. After the broccoli rotten vegetable is cooperatively fermented by bacteria and enzymes, the nutritional value of the broccoli rotten vegetable is improved, and macromolecular proteins are effectively degraded into small-molecular-weight polypeptides and amino acids after fermentation. Therefore, the bacillus provided by the invention can be used for fermenting broccoli or broccoli rotten vegetables, and has a good application prospect in preparation of feed additives.

The invention provides a bacillus subtilis strain with cellulase activity and staphylococcus aureus growth inhibition characteristics, a feed probiotic agent as well as a preparation method and application of the feed probiotic agent, and belongs to the technical field of functional microorganisms. According to the invention, a bacillus subtilis strain DXMC018 with characteristics of high cellulase activity and inhibition of growth of staphylococcus aureus is separated and screened from natural fermentation products of erythritol mother liquor, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20251866. According to the fermentation method of the strain DXMC018, erythritol mother liquor containing lysine, corn steep liquor and bran is used as a fermentation culture medium, and the strain DXMC018 is inoculated for fermentation culture. According to the fermentation method, rich probiotics are provided for feed preparation, waste liquid and waste residues can be recycled, and waste is turned into wealth.

The invention discloses an acid-resistant and high-temperature-resistant cellulose degrading bacterium and application thereof in silage, and belongs to the technical field of functional microorganism screening and application. The strain is bacillus CYC6, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36210. The strain has excellent high temperature resistance and acid resistance, and can still keep good growth and cellulase activity under the high temperature of 40-50 DEG C and the acid environment with the pH value of 3.0-4.0; meanwhile, efficient cellulose degradation capacity is achieved, and the activity of carboxymethyl cellulase reaches 17.209 + / -0.472 U / mL. The invention further provides a method for applying the CYC6 strain to silage, and the inoculum size is 1 * 10 < 8 > CFU per gram of silage raw materials. The CYC6 strain can remarkably reduce the content of neutral detergent fibers and acid detergent fibers in silage, meanwhile, the content of crude protein is increased, the fermentation quality is improved, the pH value is reduced to 3.98, and the content of lactic acid is increased to 12.03%. The technical problem that existing cellulose degrading bacteria are prone to inactivation in the high-temperature acid environment in the ensiling process is solved, and an effective solution is provided for improving the quality of silage.

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the yield of exo-cellulase of mycelia thermophila by using a transcription factor 37566. The transcription factor 37566 is knocked out from a host of the mycelia thermophila, so that the yield of the exo-cellulase can be obviously improved. Compared with an original strain, the transcription factor 37566 knockout engineering strain has the advantages that the activity of the exo-cellulase is improved by 44%, and the production cost of the exo-cellulase is effectively reduced.

The application provides a bacillus australimaris strain M04, a bacterial fertilizer, a fermentation agent and application thereof, and belongs to the technical field of microorganisms.The application provides a bacillus australimaris strain M04 with a preservation number of CGMCC No.30262.It is proved by experiments that the bacillus australimaris strain M04 has the ability of calcium dissolution, can improve the content of effective calcium in soil, improve the soil environment, can also produce auxin to promote plant growth; in addition, the bacillus australimaris strain M04 has good cellulase activity and the characteristics of salt and alkali tolerance, can effectively promote the rotting of organic fertilizer such as straw in a high-salt environment, has a positive effect on accelerating the conversion and utilization of straw returned to the field, and has a wide application prospect in the preparation of salt and alkali tolerant multifunctional biological organic fertilizer.

The invention discloses a straw feed liquid fermentation method and application thereof, and the method comprises the following steps: taking pleurotus geesteranus as a fermentation strain, preparing a seed culture medium containing inducer paper pulp by utilizing natural high cellulase activity of the pleurotus geesteranus, sterilizing, inoculating and culturing to obtain a seed solution with high cellulase activity; and inoculating the pleurotus geesteranus thallus into a straw liquid fermentation culture medium for fermentation culture, carrying out graded filtration on the fermentation liquid, and drying pleurotus geesteranus thallus solid residues to obtain the liquid fermentation straw feed. According to the method, the inducer paper pulp is added in the culture process of the seed culture medium, so that the straw utilization rate is increased by 25%, meanwhile, the enzyme activity is remarkably improved, the protein content of a fermentation product reaches 40% or above, the polysaccharide content of edible fungi reaches about 8%, and the fermentation period is shortened to 4 days. Compared with other liquid-state fermented straw feed, the liquid-state fermented straw feed has higher application value and popularization prospect, is suitable for large-scale production of high-value straw feed, effectively reduces the production cost of the straw feed, and assists green agriculture development.

The invention relates to the field of microorganisms, in particular to bacillus amyloliquefaciens MG-D2133 for high-yield digestive enzyme and application of the bacillus amyloliquefaciens MG-D2133. The preservation number of the bacillus amyloliquefaciens MG-D2133 is CGMCC (China General Microbiological Culture Collection Center) No.34353, the preservation time of the bacillus amyloliquefaciens MG-D2133 is 2025-4-25, and the bacillus amyloliquefaciens MG-D2133 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The bacillus amyloliquefaciens MG-D2133 is separated from a healthy human body and has the capacity of producing protease, amylase, lipase and cellulase, the activity of the amylase and the activity of the cellulase are remarkably higher than those of positive strains, and it is indicated that the bacillus amyloliquefaciens MG-D2133 has the huge potential of promoting food digestion and absorption and improving intestinal health.

The present application relates to the field of biotechnology, and more particularly to a kind of alfalfa transient expression cellulase and its application in Xinguan fruit leaf active substance extraction, comprising the following steps: (1) cellulase coding gene construction vector, transformation agrobacterium, culture, centrifugation, collection of bacteria, suspended in buffer, dilution;(2) the bacteria solution obtained in step (1) is used to carry out vacuum infiltration on alfalfa leaf, culture, and 1-500 μM melatonin is sprayed on alfalfa leaf, and spraying is carried out once a day;(3) the alfalfa leaf obtained in step (2) is ground, homogenized, centrifuged, and supernatant is extracted to obtain cellulase enzyme solution;Melatonin is applied in the process of expression, and the activity of cellulase obtained is significantly improved, the problems of low activity of plant expressed cellulase and extraction of leaf active substance biological enzyme are solved;And the steps are simple, the cellulase prepared does not contain endotoxin, can be used for Xinguan fruit leaf active substance extraction, and has wide application prospect.

The invention provides bacillus subtillis capable of producing amylase, protease and cellulase and application of the bacillus subtillis, the bacillus subtillis is bacillus subtillis FJAT-10612, the bacillus subtillis is preserved in China General Microbiological Culture Collection Center on August 6, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.35537. The amylase production activity of the bacillus subtillis disclosed by the invention is 166.12 U / mL, the cellulase production activity of the bacillus subtillis is 88.05 U / mL, and the protease production activity of the bacillus subtillis is 18.36 U / mL; certain tolerance to artificial gastric juice, artificial intestinal juice and cholate is achieved; when the strain fermentation liquor is used for pomace micro-storage, the content of pomace crude protein and total sugar can be remarkably increased, the cellulase activity is improved, meanwhile, the content of crude fibers, neutral detergent fibers, lignin and ammoniacal nitrogen is remarkably reduced, and an effective strain resource is provided for pomace micro-storage fermented feed.

The invention provides bacillus velezensis capable of producing amylase, protease and cellulase and application of the bacillus velezensis, the bacillus velezensis is bacillus velezensis FJAT-10502 and is preserved in China General Microbiological Culture Collection Center on August 6, 2025, the preservation address is No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The amylase production activity of the bacillus velezensis is 181.39 U / mL, the cellulase production activity of the bacillus velezensis is 28.4 U / mL, and the protease production activity of the bacillus velezensis is 18.48 U / mL; the antibacterial activity on escherichia coli and salmonella is achieved, and certain tolerance on artificial gastric juice, artificial intestinal juice and cholate is achieved; when the strain fermentation liquor is used for pomace micro-storage, the content of crude protein and total sugar of pomace can be remarkably increased, meanwhile, the content of crude fiber, acid detergent fiber, neutral detergent fiber and lignin is remarkably reduced, and an effective strain resource is provided for pomace micro-storage fermented feed.

This invention relates to the field of microbial inoculant preparation technology, specifically to a thermophilic and halophilic *Trichoderma* agent and its preparation method. The active ingredient of the *Trichoderma* MT-ST / GP agent in this invention is *Trichoderma* MT-ST / GP, deposited at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC No. 41587, and the agent contains 8 × 10⁸ viable cells. 6 ~1.2×10 7 The cellulase activity is 45-55 U / mL, the hemicellulase activity is 75-85 U / mL, and the salt tolerance concentration is 4-20 g / L NaCl. This invention improves the number of viable bacteria, enzyme activity, and salt tolerance of the inoculant by optimizing fermentation conditions (segmented temperature control, nutrient supplementation, and protective agent adaptation). It is suitable for scenarios such as agricultural waste degradation and biological treatment of high-salt environments.

The invention relates to the technical field of resources and environments, in particular to a method for controlling rumen microorganisms to stably produce methane in vitro, which comprises the following steps: regulating and controlling a nitrogen-rich substrate to account for 50-75% of the total dry weight of a system, reducing ORP (oxidation-reduction potential) to below 350mV, and enriching hydrolytic acidification bacteria Clostridium and methanogenic bacteria Methanosarcina; the pH value is adjusted to 6.9 to 7.5, and the glycolysis and Enter-Doudoroff pathway flux is improved; by taking propionic acid metabolism delta Gpropanoate less than or equal to 42 kJ / mol and butyric acid metabolism delta Gbutanoate less than or equal to 90 kJ / mol as a stable judgment standard, regulating and controlling CO2 partial pressure and enhancing the expression of an acetic acid nutritional type methanogen gene mtaABC and a hydrogen nutritional type methanogen gene mtrH, wherein the acetic acid metabolism delta Gpropanoate is less than or equal to 42 kJ / mol and the butyric acid metabolism delta Gbutanoate is less than or equal to 90 kJ / mol; for the condition that the indexes do not reach the standard, optimization is carried out by increasing the proportion of nitrogen-rich substrates and regulating and controlling the hydrogen partial pressure to be 24.41 kPa or below. According to the method disclosed by the invention, the methane yield is stably improved and the activity of hemicellulase and cellulase is remarkably enhanced by regulating and controlling microbial communities, metabolic pathways and functional genes, so that the problem of methane yield fluctuation caused by improper in-vitro domestication is effectively solved, and a reliable technical scheme is provided for efficiently converting cellulose biomass into biological methane.