48 results about "Exocellulase activity" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention discloses a novel application of bacillus subtilis WJL-2, and relates to the technical field of microorganisms. The invention discloses a novel application of bacillus subtilis WJL-2. The preservation number of the bacillus subtilis WJL-2 is GDMCC65111, and the preservation number of the bacillus subtilis WJL-2 is GDMCC65111. The new application specifically comprises the following steps: improving soil, regulating and controlling the content of total nitrogen and organic carbon in the soil, regulating and controlling the activity of amylase / urease / cellulase in the soil, regulating and controlling the content of ammonium nitrate nitrogen in the soil, regulating and controlling the mineralization rate of the soil, regulating and controlling the nitration rate of the soil and regulating and controlling the ammoniation rate of the soil. After the strain WJL-2 is inoculated into the soil, the total nitrogen and organic carbon content of the soil is reduced, and the C / N is reduced; the activity of soil amylase is reduced, and the activity of urease and cellulase is increased; the content of ammonium nitrogen in the soil is in a trend of firstly increasing and then decreasing, and the content of nitrate nitrogen is continuously increased; the mineralization rate of soil organic nitrogen is accelerated, the ammoniation rate is in a trend of increasing first and then decreasing, and the nitration rate is in a trend of decreasing first and then increasing.

The invention discloses bacillus subtilis MY-01 as well as a microbial preparation and application of the bacillus subtilis MY-01. The strain (preservation number is CCTCC NO: M 20242596) can efficiently degrade cellulose, total sugar and nicotine in tobacco stems and remarkably improve sensory indexes such as aroma quality and aroma amount, and the cellulase activity is greatly improved when the strain and sodium carboxymethyl cellulose act synergistically. The microbial preparation is obtained through the processes of strain activation, fermentation culture and microbial agent preparation, and when the microbial preparation is applied to tobacco stem treatment, the technical breakthrough of reduction of the cellulose content, increase of the filling value and reduction of the tar release amount is achieved through the steps of water content adjustment, preparation spraying, sealed fermentation, low-temperature drying and the like. Compared with a traditional method, the technology has the advantages that the treatment period is greatly shortened, the cost is remarkably reduced, the COD value of wastewater is reduced, the cigarette quality and the production environmental protection property are remarkably improved, and the industrial application prospect is wide.

The invention relates to the field of microorganisms, and provides an acid-producing propionibacterium BL8 with hypoglycemic and anti-aging functions and application of the acid-producing propionibacterium BL8, the acid-producing propionibacterium BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No. 33134. The acid-producing propionibacterium BL8 disclosed by the invention can be used for inhibiting the activity of DPP4, promoting insulin secretion of INS-1, and inhibiting the activity of acetylcholin esterase and excellent cellulase activity. The blood sugar can be reduced; the memory is improved, and the Alzheimer disease is prevented and relieved; and the traditional Chinese medicine composition has relatively high research and application values in the aspects of reducing blood sugar and resisting aging.

The invention discloses a fruit and vegetable grafting wound healing nutrient solution containing polysaccharide complex enzyme and a preparation method, belongs to the technical field of agricultural biochemical preparations, and aims to solve the technical problems of low removal efficiency of necrotic tissues at grafting wounds and high risk of microbial infection. According to the technical scheme, the nutrient solution is characterized by being prepared from the following raw material components in parts by weight: 0.3 to 0.8 part of polysaccharide, 1 to 5 parts of cellulase, 0.5 to 2.5 parts of pectinase, 1 to 5 parts of glycerol and 90 to 100 parts of water. Wherein the pH value of the nutrient solution is adjusted to 5.0-6.5 by using a phosphate buffer agent, the polysaccharide is composed of chitosan and sodium alginate, the mass ratio of the chitosan to the sodium alginate is 1: 0.5-1: 2, the activity of cellulase in the nutrient solution is 100-500 U / mL, and the activity of pectinase in the nutrient solution is 50-250 U / mL. The nutrient solution is mainly used for promoting fruit and vegetable grafting wound healing, and application verifies that the nutrient solution can effectively improve wound healing quality and reduce infection risk in the grafting process of fruits and vegetables such as cucumbers, eggplants, peppers, tomatoes, litchis and oranges, and has a good application effect.

According to the invention, lactobacillus rhamnosus UP180 with relatively strong cellulase activity and lactobacillus paracasei ID001 with relatively strong protease activity are combined to prepare composite probiotic powder, the composite probiotic powder is used for jointly fermenting the endothelium corneum gigeriae galli formula, and meanwhile, a polypeptide capable of improving the digestive enzyme activity of Caco-2 cells is found from a fermentation product. The compound strain fermentation effectively decomposes macromolecular proteins in the endothelium corneum gigeriae galli formula and converts the macromolecular proteins into small molecular peptides, effective active ingredients in the formula are fully released, and the conversion effect is obviously superior to that of single strain fermentation. Besides, the endothelium corneum gigeriae galli composition leavening prepared by the invention can improve nutrient absorption of the rats by improving enzyme activity and metal ion chelation in intestinal tracts of the rats in the weaning period and promote growth of the rats in the weaning period, and has a potential application prospect in the field of nutrient absorption of children.

The invention discloses an application of graphene oxide in degrading straws by synergistic trichoderma. The application comprises the following steps: crushing the straws, and mixing the crushed straws with soil; spraying the trichoderma suspension into the mixture of the straws and the soil; spraying the graphene oxide hydrosol into a mixture of trichoderma, straws and soil; the concentration of the trichoderma suspension is 0.5 * 10 < 5 > cfu / mL to 5 * 10 < 5 > cfu / mL; the concentration of the graphene oxide hydrosol is 5 mg / L-50 mg / L; the water content of the mixture of the trichoderma, the straw and the soil is 50-70 wt%; trichoderma is treated through graphene oxide hydrosol, graphene oxide can promote mycelial growth of the trichoderma, meanwhile, lignin peroxidase activity and cellulase activity of the trichoderma can be promoted, and the straw degradation efficiency is improved; the expressions in the aspects of straw phenotype, mass loss rate, mycelial growth, cellulase and ligninase activity in the straw degradation process are greatly improved.

The invention relates to a cellulase activity detection device and method, and belongs to the technical field of enzyme activity detection.The cellulase activity detection device comprises a first box body, a second box body installed in the first box body, a controller and a temperature control device installed on the side portion of the first box body, and the temperature control device is connected with the second box body through a pipeline; the turbulent flow mechanism is arranged in the second box body, and the air with the preset temperature provided by a temperature control device is disturbed through the turbulent flow mechanism, so that the temperature in the second box body is kept within a preset temperature threshold range; the air supply amount and the air supply temperature provided by the temperature control device are controlled through the controller. By arranging the turbulent flow mechanism and the temperature control device, the activity of cellulase can be better controlled and detected, so that the detection precision of the cellulase activity can be improved.

A process for upcycling spent coffee grounds into an antioxidant dietary fiber involves a microwave assisted extraction of the spent coffee grounds in water at a temperature up to 200° C. for a time of at least 5 min, followed by an enzymatic assisted extraction of the spent coffee grounds in an aqueous solution including an enzyme having endo-beta-glucanase activity and / or an enzyme having cellulase activity. The process further involves recovering soluble matter. Prior to the microwave assisted extraction step, the spent coffee grounds are subjected to a pulsed electric field treatment or a high-pressure extraction or both.

The present invention provides a Pleurotus citriodora strain for co-producing ergothioneine and polypeptides, a preparation method and an application thereof, belonging to the field of microbial technology; the strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on June 13, 2025, with the deposit number: GDMCC NO.66504. After the Pleurotus citriodora strain was induced, the EGT production of the strain was doubled compared with the original strain, the cellulase activity was increased by about 38.45% compared with the original strain, and the mushroom protein content was increased by 59.46% compared with the original strain. During the fermentation process, the EGT production using bran as the carbon source reached 2.8 times that of glucose; after expanded culture in a 5L fermenter, the EGT production could reach 560 mg / L, and further application of the dual-stage acid-base stress strategy to the fermenter could increase the EGT production to 741.38 mg / L.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

The application discloses a high-efficiency lignocellulose-degrading filamentous fungus mutant strain and application thereof. The high-efficiency lignocellulose-degrading filamentous fungus mutant strain is formed by knocking out a wild-type filamentous fungus Podospora anserina photoperiod gene PaFrq. The application firstly finds that the filamentous fungus Podospora anserina with the knocked-out photoperiod gene PaFrq has the advantages of delayed aging, prolonged life cycle, improved growth speed, higher efficiency in degrading lignocellulose, especially improved cellulase activity in dark conditions, higher efficiency in degrading lignocellulose, and important significance for a fungus fermentation industry, especially for more efficient conversion of fungal biomass.

The application discloses a method for improving cellulase activity by using a combination of an endogenous promoter and an associated 5' UTR of Trichoderma reesei and an engineering strain constructed by the method. TrAE The 5' UTR upstream of the TrAE gene TrAE Overexpressing a transcriptional regulator xyr1 The expression cassette of the gene is transformed into Trichoderma reesei Δ ku70 Rut‑C30 The application also discloses a high-yield cellulase Trichoderma reesei strain QEaeuX, and a method for producing high-activity cellulase by using the strain. ku70 Rut‑C30 The cellulase activity produced by the strain in a 96 h shake flask culture is 2.6 IU / mL, which is 101% higher than that of the original strain Δ The application has a good industrial development and application prospect.

This solution discloses a high-temperature fermentation strain for Maotai-flavor baijiu brewing and its application in the field of functional microbial technology. The strain was isolated from mature Maotai-flavor baijiu mother koji and identified as *Bacillus cereus* XJ143 by 16S rRNA sequencing, possessing high cellulase activity. This strain is heat-resistant and suitable for the Maotai-flavor step-by-step koji-making process, solving industry problems such as insufficient decomposition of raw materials in traditional koji, insufficient pyrazine roasting aroma, and large batch-to-batch quality fluctuations in naturally produced koji. It can directly prepare liquid seed culture or solid inoculum for mother koji expansion, high-temperature koji, and mash fermentation, exhibiting strong adaptability for industrial production. *Cropensis ivory* XJ112 possesses high protease and amylase activity. The combination of *Bacillus cereus* XJ143 and *Cropensis ivory* XJ112 combines the secretion abilities of protease, amylase, and cellulose, efficiently breaking down wheat cell walls and simultaneously decomposing raw starch and protein.

The application discloses a kind of forestry waste treatment methods for improving forest carbon sequestration capacity, belong to forestry waste processing technical field, the method is to carry out composting after forestry waste is returned to forest, specifically including material crushing, carbon-nitrogen ratio adjustment, moisture content and pH adjustment, heap preparation and maturity and the like step, by preparing enzyme activity control agent and mixing into material slag to compost, the high concentration glucose produced in high temperature stage decomposition is absorbed by the prepared enzyme activity control agent, to reduce the inhibitory effect of high concentration glucose on cellulase, while the control agent further improves cellulase activity, further control to water, pH and the like condition, guarantee composting efficiently, improve composting rate and degree of maturity, the fertilizer obtained by composting can effectively improve the physicochemical properties of soil in forest, promote the growth of forest trees, improve stand quality and carbon sequestration capacity, the forestry waste treatment method of the application has important significance to the resource utilization of forestry waste and forest carbon sequestration and sink.

The invention provides a method for relieving inhibition of phenolic compounds on cellulase in a wood fiber saccharification system. The method is used for inhibiting covalent cross-linking of phenolic compounds and cellulase, and comprises one or more of reduction of the content of the phenolic compounds in a system, enzymolysis saccharification under an anaerobic condition and addition of an inhibitor for competitive binding of the phenolic compounds. The method for reducing the content of the phenolic compounds specifically comprises the following steps: carrying out alkaline pretreatment on a wood fiber raw material and / or carrying out water extraction treatment on the wood fiber raw material / a pretreated solid sample. The inhibitor is an amino-containing compound, and the amino-containing compound is ethylamine, L-lysine or cysteine. The method is a technical scheme provided based on a new discovery of an inventor on a mechanism of inhibiting cellulase activity by phenolic compounds in a lignocellulose saccharification system, the inhibition of the phenolic compounds on cellulase in the saccharification step is fundamentally relieved, and the saccharification efficiency of lignocellulose is remarkably improved. The invention also provides a method for remarkably improving the saccharification efficiency of the wood fibers, and the method inhibits covalent crosslinking of the phenolic compounds and the cellulase, so that the saccharification efficiency is remarkably improved, and the method has important industrial application value.

The invention provides a Trichoderma reesei engineering bacterium for high yield of beta-glucosidase, which is characterized in that a Trichoderma reesei SN1 strain is used as a starting strain, and an expression cassette for knocking out a cre1 gene, a constitutive expression cassette of an aspergillus niger source bglA gene expressed by a promoter cbh1 and a constitutive expression cassette of a 130442 gene expressed by the promoter cbh1 are constructed in a genome of the Trichoderma reesei SN1 strain. Through the knockout of the cre1 gene, the overexpression of the aspergillus niger source bglA gene and the overexpression of the 130442 gene, the yield of the beta-glucosidase in the trichoderma reesei is improved synergistically. Glucose is taken as a raw material, a transglycosylation reaction can be catalyzed by directly utilizing a crude enzyme solution of trichoderma reesei engineering bacteria with high yield of beta-glucosidase to prepare beta-disaccharide, and the beta-disaccharide can be taken as an inducer for cellulase production; a crude enzyme liquid with higher cellulase activity and beta-glucosidase activity is obtained by supplementing an inducer and carrying out fed-batch fermentation.

The invention relates to the technical field of new strains for degrading cellulose, in particular to a pseudoxanthomonas strain JC1303 and application thereof. Through whole genome sequencing and function annotation, it is systematically revealed for the first time that the strain carries a complete cellulose degrading enzyme system including incision beta-1, 4-glucanase, cellulase and beta-glucosidase, has a plurality of central metabolic pathways supporting efficient degradation and utilization of cellulose, and can be used for degrading cellulose. And specific gene resources are determined through generic genome analysis. The strain has the remarkable technical effects that the strain shows higher cellulase activity after being cultured for 5 days, and cellulose materials such as agricultural wastes and the like can be stably and efficiently degraded in a salt-containing environment by virtue of the unique genetic background and marine source characteristics of the strain.

The invention discloses a fermentation engineering bacterium Lactobacillus plantarum JY003 for wine-processed traditional Chinese medicine decoction pieces, the preservation number of the fermentation engineering bacterium is CCTCC (China Center For Type Culture Collection) NO: M 20252157, the preservation date is September 29, 2025, and the classification name of the fermentation engineering bacterium is Lactobacillus plantarum JY003. The preserved strain can tolerate alcoholic strength of 10-15%, has both pectinase activity and cellulase activity, can keep high cellulase activity and pectinase activity under the yellow wine processing condition, further promotes plant cell wall degradation, enables active ingredients in cells to be released and dissolved out, can be used as a wine-processed traditional Chinese medicine decoction piece fermentation engineering strain, and has a wide application prospect. The method is used for preparing wine-processed traditional Chinese medicine decoction pieces.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

The invention relates to the technical field of agricultural microorganisms, in particular to bacillus rugosii and application thereof. The bacillus rugosus disclosed by the invention is preserved in the China Center for Type Culture Collection on October 25, 2023, and the preservation number of the bacillus rugosus is CCTCC (China Center for Type Culture Collection) NO: M20232022. The bacillus rugosii and the microbial agent provided by the invention have a very strong antagonistic effect on phytophthora capsici, and have a very good prevention and treatment effect on phytophthora blight of capsici; the ecological adaptability is high; the organic phosphorus decomposing effect is good, and the amylase decomposing activity and the cellulase decomposing activity are remarkable; the fertilizer has strong drought-resistant and saline-alkali-resistant capabilities, and can promote the growth of peppers under saline-alkali conditions, improve the agronomic traits of the peppers and promote the yield increase of the peppers.

A method for upgrading waste coffee grounds into antioxidant dietary fibers comprises the following steps: a) performing microwave-assisted extraction (MAE) on a waste coffee grounds (SCG) material in water at a temperature of at most 200 DEG C for a time of at least 5 minutes; and then b) subjecting the waste coffee grounds material extracted according to step a) to enzyme-assisted extraction (EAE) in an aqueous solution comprising an enzyme having endoglucanase and / or cellulase activity, and recovering soluble substances. In a preferred embodiment, the SCG is pre-treated prior to the MAE extraction step, the pre-treatment being selected from pulsed electric field treatment or high voltage extraction, or both.

The application discloses a shrimp intestinal tract Tenacibaculum lutimaris strain and application of the shrimp intestinal tract Tenacibaculum lutimaris strain in shrimp culture, and has the characteristics that the strain is BG12 strain, is classified and named as Tenacibaculum lutimaris, is preserved in the China General Microbiological Culture Collection Center on July 8, 2022, and has a preservation number of CGMCC No. 25267, the application of the shrimp intestinal tract Tenacibaculum lutimaris strain in preparation of a Vibrio parahaemolyticus inhibitor and in preparation of a probiotic additive for shrimp culture, and the advantages are that the shrimp intestinal tract Tenacibaculum lutimaris strain has amylase and cellulase activities, and can improve the survival rate of shrimp culture and the anti-invasion ability of shrimp culture to Vibrio parahaemolyticus.

The invention relates to the technical field of microorganisms, in particular to bacillus altitudinis as well as a complex microbial inoculant and application thereof. The invention provides bacillus altitudinis, which is derived from intestinal tracts of earthworms, is named as DBN-EW28 and is preserved in China General Microbiological Culture Collection Center on February 14, 2025, the preservation number is CGMCC No.33530, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing. The bacillus altitudinis DBN-EW28 strain disclosed by the invention has relatively high cellulase activity and antibacterial property, and is used for degrading crop straws when being combined with other strains, so that the contents of cellulose and hemicellulose in the crop straws are remarkably reduced, and the degradation effect of straw lignocellulose and the conversion rate of reducing sugar are greatly improved.

The present invention discloses Penicillium cyclopium with high efficiency in degrading straw fiber and its application. The present invention uses nitrosoguanidine method to mutagenize the Penicillium cyclopium strain MP-03(2554-2) (CGMCC No. 41542) to obtain a strong corn straw-degrading mutant strain ΔMP-03(455-1) (CGMCC No. 41541). By optimizing the nitrogen-carbon ratio, culture time, temperature and pH range conditions of the optimal enzyme-producing medium, compared with the non-mutant strain 2554-2, the 455-1 mutant strain significantly improves the degradation rate of corn straw, the activities of endo-cellulase and exo-cellulase. The present invention optimizes the scale-up culture conditions of the 455-1 mutant strain, and develops a new type of organic coupling cultivation technology such as the fermentation broth of the 455-1 mutant strain + corn straw + organic fertilizer for protected vegetables, which significantly improves the growth, root development and photosynthetic efficiency of protected tomatoes, peppers and cucumbers, as well as the resistance to soil-borne oomycete diseases.

The invention relates to the field of microorganisms, in particular to bacillus subtilis MG-D587 for high-yield digestive enzyme and application of the bacillus subtilis MG-D587. The preservation number of the bacillus subtilis MG-D587 is CGMCC (China General Microbiological Culture Collection Center) No.34354, the preservation time of the bacillus subtilis MG-D587 is 2025-4-25, and the bacillus subtilis MG-D587 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The bacillus subtilis MG-D587 is separated from a healthy human body and has the capacity of producing protease, amylase, lipase and cellulase, the activity of the amylase and the activity of the cellulase are remarkably higher than those of positive strains, and it is indicated that the bacillus subtilis MG-D587 has the huge potential of promoting food digestion and absorption and improving intestinal health.

The invention relates to the technical field of wood protective agents, and discloses ammonium bis (oxalato) borate and a preparation method and application thereof in wood protection, and the preparation method of the ammonium bis (oxalato) borate comprises the following steps: oxalic acid, boric acid and water are mixed and stirred to obtain a mixed solution; and mixing the mixed solution with an ammonium compound, and carrying out heating reaction to obtain ammonium bis (oxalate) borate. The ammonium bis (oxalato) borate prepared by the method has a remarkable nitrogen-boron synergistic flame retardant effect, the flame retardant property of wood treated by the ammonium bis (oxalato) borate can reach the B1-level flame retardant standard, and the heat release and smoke generation amount are remarkably reduced; wherein the boron element can destroy the enzyme system of wood-rotting fungi (inhibit the activity of cellulase) and interfere the digestive system of termites (destroy the intestinal flora) at the same time, so that the environment-friendly and low-toxicity characteristics of the traditional boron system are reserved, and the effects of corrosion prevention and insect prevention are achieved. In addition, the preparation process provided by the invention is green, environment-friendly, low in cost and easy for large-scale popularization and application.

The present application relates to the field of microorganisms, and provides a propionibacterium acidifaciens BL8 with functions of reducing blood sugar and resisting aging, and application thereof, the propionibacterium acidifaciens BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No.33134.The propionibacterium acidifaciens BL8 of the present application can inhibit DPP4 activity, promote INS-1 insulin secretion, inhibit acetylcholinesterase activity and superior cellulase activity.Can reduce blood sugar;Improve memory, prevent and relieve Alzheimer's disease;Promote cellulose absorption, improve constipation, and have high research and application value in reducing blood sugar and resisting aging.

The invention discloses bacillus, a microbial inoculum and application of the microbial inoculum in fermentation of broccoli or broccoli rotten vegetable, and relates to the technical field of agricultural waste recycling. The bacillus sp. Is screened and separated from cow dung subjected to high-temperature composting, can grow under a high-temperature condition, and has remarkable protease and amylase activity and weak cellulase activity. After the broccoli rotten vegetable is cooperatively fermented by bacteria and enzymes, the nutritional value of the broccoli rotten vegetable is improved, and macromolecular proteins are effectively degraded into small-molecular-weight polypeptides and amino acids after fermentation. Therefore, the bacillus provided by the invention can be used for fermenting broccoli or broccoli rotten vegetables, and has a good application prospect in preparation of feed additives.

The invention provides a pleurotus citrinopileatus strain for co-production of ergothioneine and polypeptide, and a preparation method and application thereof, and belongs to the technical field of microorganisms. The strain is preserved in Guangdong Microbial Culture Collection Center on June 13, 2025, and the preservation number of the strain is GDMCC NO. 66504. After the pleurotus citrinopileatus strain is mutagenized, the EGT yield of the strain is increased by two times compared with that of an original strain, the cellulase activity is increased by about 38.45% compared with that of the original strain, and the mushroom protein content is increased by 59.46% compared with that of the original strain. In the fermentation process, the yield of EGT (epigallocatechin gallate) is 2.8 times that of glucose by taking bran as a carbon source; the yield of the EGT can reach 560 mg / L through amplification culture of a 5L fermentation tank, and the yield of the EGT can reach 741.38 mg / L by further applying a two-stage acid-base stress strategy to the fermentation tank.