80results about How to "Increase protease activity" patented technology

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

The invention discloses a method for producing fermented bean curd through multi-strain mixed fermentation. The method comprises the following steps of: (1) preparing raw fermented bean curd, namely preparing the raw fermented bean curd from soybean; (2) culturing bacteria at the early stage, namely inoculating mixed strains comprising mucor, Aspergillus oryzae and Aspergillus niger into the raw fermented bean curd to culture bacteria at the early stage to obtain the fermented bean curd blanks; (3) pickling by using salt, namely placing the fermented bean curd blanks obtained in the step (2) into a rubber box, scattering salt layer by layer, and pickling with the salt to prepare salty blanks; (4) fermenting at the later stage, namely placing the pickled fermented bean curd salty blanks into a bottle, inoculating flavor-increasing yeast into wine liquid of semifinished products, and fermenting at the later stage; and (5) obtaining the fermented bean curd. By the method, the flavor of the fermented bean curd products can be enriched and the fermenting period of the fermented bean curd is shortened.

The invention discloses a composite microbial inoculum fertilizer. The invention is characterized in that the fertilizer is prepared from the following components in parts by weight: 6-8 parts of composite microbial inoculum, 25-35 parts of potassium sulfate, 40-55 parts of urea, 35-50 parts of calcium superphosphate, 150-175 parts of biomass power plant ash, 300-330 parts of sawdust, 200-250 parts of biological carbon, 100-115 parts of medical stone powder, 120-125 parts of zeolite powder and 7-9 parts of soil conditioner. The effective components of the composite microbial inoculum comprise Bacillus licheniformis, Bacillus pumilus, Bacillus lateraporus, Bacillus megaterium and silicate bacterium. The composite microbial inoculum is added to the product to improve the microflora distribution and microenvironment in soil; and the abundant generated derivatives chelate or block the direct damage of heavy metals to the tobacco to some extent, and have the function of restoring the polluted soil. The soil conditioner contains bowlder which contains abundant microelements beneficial to the human body, and the microelements are absorbed by the crops and converted through the food chain so as to be beneficial to the human body; and meanwhile, the adsorption component can effectively adsorb and solidify toxic heavy metal elements in soil, reduce the heavy metal elements absorbed by the crops, and enhance the crop yield.

The invention discloses a method for preparing a fish skin and scale collagen peptide, which comprises: (1) starter propagation; (2) high temperature skin and scale collagen peptide extraction; (3) heat preservation and enzymolysis; (4) separation; (5) high molecular weight protein removal; and (6) spray drying to obtain finished powder product of the fish skin and scale collagen peptide. The method can obviously reduce collagen peptide production cost; and the obtained collagen peptide is white and odorless powder containing less than 0.6 percent of ash content and more than 95 percent of peptide segments with a molecular weight of less than 3,000Da. The collagen peptide is free from fishy smell and odors.

The invention discloses a germinated brown rice rich in bioactive peptide and a production method thereof, belonging to the food biotechnical filed, and also belonging to the technical field of healthfood production. The germinated brown rice is characterized in that paddy is placed in culture solution which contains 30-80mu mol / L of gibberellin, 2-10m mol / L of NaCl and 0.6-3.0 m mol / L of MnSO4 to be cultured for 36-96h after husking, selection, disinfection and soaking. In the duration of germination, vent processing is carried out in the case of ventilatory capacity being 0.1-3.0L.min<-1> and the temperature being 16-40 DEG C, finally the germinated brown rice is obtained after cleaning, enzyme destruction and drying. The method has simple process and strong maneuverability, and is suitable for industrialized production; in addition, the produced germinated brown rice is a health food safe to eat, with the peptide content being 10-13mg.g<-1>.

The invention discloses a bacillus subtilis H8-3 and application thereof in preparation of a decomposed inoculant. The bacillus subtilis H8-3 is deposited with the CCTCC at Oct 21, 2013 in Hubei Wuhan university and is assigned the accession number CCTCC No:M 2013483. The bacillus subtilis H8-3 is high-temperature-resistant, can survive at 30-60 DEG C, is high in protease activity and is quick in growth. The decomposed inoculants prepared from the bacillus subtilis H8-3 is high-temperature-resistant and can secrete protease and lipase for high-efficiently fermenting animal wastes. Compost prepared through the decomposed inoculant is less in environmental pollution, is economical and is easy to use. When a product after compost treatment is subjected to subsequent treatment, the product can be employed as an organic fertilizer for achieving an object of turning waste into resources.

Methods and kits for monitoring kidney function, and detecting kidney dysfunction and transplant related disease and rejection are disclosed. The method involves analyzing a sample, such as a urine sample, containing protein from an animal for fragments of β2-microglobulin, wherein the presence of specific β2-microglobulin fragments is indicative of kidney dysfunction and transplant rejection. In another embodiement, urine samples from an animal are tested for protease activity, such as cathepsin D or napsin A, wherein increased protease activity compared to a control sample is indicative of kidney dysfunction.

The present invention relates to a mutant strain of Aspergillus sojae with an enhanced protease activity and a preparation method of a natural taste enhancer using the same. More specifically, the present invention relates to the mutant strain of Aspergillus sojae with an enhanced protease activity is obtained by selecting a strain with high protease activity and treating it with N-methyl-N′-Nitroso-N-nitrosoguanidine (NTG) and inducing a mutation through irradiating, and a preparation method of a natural taste enhancer using protein hydrolysate obtained by hydrolyzing the protein sources with their cultures.

The invention relates to a method for preparing and applying fish gravy quickly-brewed sauce, which comprises the following steps: mincing fresh fish or fish leftovers; uniformly mixing the minced fish, bean pulp and bran to be used as a ferment raw material; and adding aspergillus oryzae to carry out solid aerobic fermentation to prepare fish gravy quickly-brewed sauce. The fresh fish or the fish leftovers are used as raw materials, after the raw materials are cut into blocks and minced, the fish gravy quickly-brewed sauce, water and salt are added to be fermented at 45 DEG C-50 DEG C for 10 days to 15 days; and fermented fish gravy fermentation liquor is filtered and sterilized to obtain a fish gravy finished product. The sauce has the characteristics that the enzyme activation of protease is high and an enzyme system is more suitable for the enzymolysis of the fish. Compared with the prior soy sauce added quickly-brewed fish gravy, the sauce quickly-brewed fish gravy has the characteristics of shorter production period, lower production cost and better flavor of fish gravy produced by ferment.

The invention provides a fertilizer for cultivation of tobacco. The fertilizer consists of the components by weight as follows: 0.5-1.5 parts of a composite microbial agent, 3-7 parts of an activating agent, 3-7 parts of binder, and 86-92 parts of nitrogen, phosphorus and potassium fertilizer. The effective components of the composite microbial agent are Bacillus licheniformis, Bacillus pumilus, Brevibacillus laterosporus, Bacillus megatherium and Silicate bacteria. The invention further provides a preparation method and an application of the fertilizer. The product provided by the invention can remarkably improve crumb-structure of soil for cultivation of tobacco, and microflora distribution and micro-environment of the soil. A plenty of derivatives are generated, thereby chelating or blocking damage of heavy metals, and absorption and assimilation of tobacco to nutrient elements are improved, the drought resisting and disease resisting abilities of tobacco are enhanced, damage of diseases and insects caused by continuous cropping of tobacco in successive years are effectively prevented, and in particular, tobacco mosaic virus and brown spot disease are specially prevented and treated. According to the invention, the raw materials are low in cost and easy to obtain and the preparation and using methods are simple and easy to operate.

The invention provides a biological water flushing fertilizer. The biological water flushing fertilizer is prepared by mixing a microorganism strain and an organic fertilizer, wherein the microorganism strain is obtained by carrying out strain liquid fermentation on bacillus subtilis, brevibacillus laterosporu and bacillus mucilaginosus; the biological water flushing fertilizer is composed of the following components in percentage by mass: 10%-20% of the microorganism strain, 20%-30% of humic acid, 30%-60% of calcium nitrate or phosphorus nitrate, 5%-10% of boric acid, 5%-12% of magnesium sulfate, 5%-8% of ferrous sulfate, 1.5%-5% of organic matters, 2%-6% of sulfur powder, 0.5%-1.0% of bentonite and 1%-5% of an inductive agent. The preparation method comprises the following steps: liquid fermentation of the strain, adsorption of fermented strain liquid, and preparation of the biological water flushing fertilizer. According to the biological water flushing fertilizer, the nutritional value of fertilizer raw materials is obviously improved and the fertilizer effect is rapidly improved; the fertilizer utilization efficiency effect and the effect of preventing diseases and increasing the output are improved. Meanwhile, the soil physical and chemical properties are also improved; the hardening is eliminated, the soil is loosened, the pH value of the soil is adjusted and the soil structure is improved.

The invention relates to bacillus subtilis and an application thereof, and belongs to the technical field of biological engineering. The invention provides a bacillus subtilis strain KF-2 with a preservation number of CGMCC No. 7690. Besides, the invention provides a method for applying the bacillus subtilis in fermentation production of active peptide-rich protein feed. A plurality of indexes are determined after soybean meal is fermented by the strain. Crude protein content reaches over 51%; acid soluble protein content reaches over 6%; viable counts reach 2 billion per gram of the fermented soybean meal; and volatile salt-based nitrogen is lower than 50 mg / 100 g.

The invention discloses a method for improving the protease activity of soy sauce koji, which carries out koji making by mixing soybean whey protein peptide, flour or bran, and culture with the soybean after stewing, wherein, the soybean whey protein peptide, the flour or the bran, and the culture are respectively 0.01 to 0.1 percent, 10 to 80 percent, and 0.1 to 0.6 percent of the weight of soybean, thus achieving the aim at improving the protease activity in the soy sauce koji. The method not only changes the existing soy sauce koji making technology and significantly improves the protease activity of the soy sauce koji by 20 to 30 percent, but also obviously improves the protein utilization rate and the amino acid transformation ratio of soy sauce fermentation, with prominent social and economic benefit.

The invention discloses a preparation method for an organic material decomposition agent to solve problems comprising strain unicity, less types, bad strain adaptability, unstable effects and a low degree of industrialization in the prior art. According to the method, bacillus subtilis, aspergillus oryzae and brewer's yeast are selected and used. After steps of preparing nutrition mediums, culturing individually and controlling inoculation quantities, fermentation temperatures, fermentation times and adjusting pH parameters, concentrating and drying, and adjusting ratios to prepare the decomposition agent capable of making crop straws return to the field in situ. According to the method, the bacillus subtilis, the aspergillus oryzae and the brewer's yeast are selected. The three stains have good symbiosis and metabiosis effects and have no antagonistic effect, thereby enhancing protease activity of products and capability of antagonizing crop pathogenic bacteria, and playing functions of bio-control and plant growth promotion.

The invention discloses a method for preparing broad bean sauce through synergistic fermentation of multiple bacteria. The method comprises the following steps: (1) soaking broad beans; (2) steaming the broad beans; (3) mixing the broad beans with flour, inoculating with aspergillus oryzae and aspergillus niger spore powder to obtain koji; (4) turning over the koji; (5) fermenting in brine; and (6) supplementing lactobacillus plantarum and saccharomyces rouxii, and perfomring synergistic fermentation. According to the invention, through inoculation and fermentation, the production period of the broad bean sauce is shortened, and the product quality is stabilized; and by supplementing preferred mycete, the synergistic effect of fermentation is enhanced, and the volatile flavor compounds andthe nutrient quality of the broad bean sauce are improved.

The invention discloses a novel safe environmentally-friendly feed additive for the livestock and poultry industry. The novel safe environmentally-friendly feed additive is prepared from pheophytin, betaine, metaplexis japonica extract, oxidized matrine, yucca saponin, rose-boot extract, flos caryophylli powder, clinoptilolite powder and illite powder according to a scientific ratio. The novel safe environmentally-friendly feed additive has activation, adjustment and quick-acting functions, improves animal immunity, maintains the balance of florae in a livestock intestinal tract, improves muscle quality, a culture environment and an organic decomposition rate, reduces an ammonia concentration of air in an animal house and realizes deodorization. In breeding, the novel safe environmentally-friendly feed additive can fully avoid antibiotic medicines, reduce mycotoxins, prevent enterorrhea and diarrhea, resist stress and coccidium, improve a feed conversion ratio and an egg yield, and eliminate heavy metal and drug residues.

The invention discloses novel red preserved bean curd and a production process thereof and belongs to the field of preserved bean curd production processes. The raw materials of the novel red preserved bean curd comprise soybeans, red beans and Chinese dates. The production process comprises the following steps: preparing a blank, performing early-stage bacteria culture, performing early-stage fermentation and salting; standing in a dark environment for 1-2 months, thereby obtaining finished red preserved bean curd; and bottling for selling. According to the red preserved bean curd, the raw materials and production process of making the traditional red preserved bean curd are changed, so that the red preserved bean curd has the color of the traditional red preserved bean curd, the interior and exterior of the preserved bean curd are red, the taste is good, especially the nutritional ingredients such as dietary fibers, vitamin B group, vitamin E and carotene are increased, and the red preserved bean curd has nutritional and health care effects.

The invention provides a water-soluble medicinal fertilizer containing a strong-antagonism anti-rice blast bacterial strain, a preparation method and application of the medicinal fertilizer. The medicinal fertilizer is prepared from the following constituents in parts by weight: 55-85 parts of an HR (Hypersensitive Response) antibiological inoculant, 10-30 parts of polyglutamic acid, and 5-15 parts of a plant cell stabilizer, wherein the HR antibiological inoculant comprises bacillus subtilis-w3 with an effective viable count of 7.0-9.0*10<9> cfu / g. The medicinal fertilizer disclosed by the invention can achieve a good biological control effect on rice blast, banded sclerotial blight and the like, significantly promotes growth of the rice, increases number of productive ears, setting percentage and thousand seed weight of the rice, and remarkably improves the vegetable yield and the quality.

The invention aims to provide a fragrant Xinjiang little white apricot ferment and a preparation method thereof. The fragrant Xinjiang little white apricot ferment takes Xinjiang little white apricotsas a raw material and white granulated sugar as an auxiliary material and is fermented by adding a composite fermentation microbial agent prepared from Pasteur acetobacter, lactococcus lactis and pichia caribii, and under the synergistic fermentation effect of the composite fermentation microbial agent, the fragrant Xinjiang little white apricot ferment with stable and consistent quality is obtained. The compound fermentation bacterial agent can ensure the structure and quantity stability of the fermented microbial communities, greatly shorten a fermentation period, improve the product quality and the yield and increase the fragrance of the compound fermentation bacterial agent, and has wide practicability and development value.

A liquid detergent composition containing (A) 10 to 70 mass % of a nonionic surfactant, (B) 1 to 15 mass % of an anionic surfactant, (C) 0.01 to 2 mass % of a protease, and (D) 0.001 to 0.1 mass % of at least one compound selected from the group consisting of thiazole-based compounds and sulfur-containing amino acids.

The present invention relates generally to a method of detecting a risk of the progression of a pre-invasive neoplasia of the glandular epithelium. More particularly, the present invention provides a method of detecting a risk of the progression from a pre-invasive breast neoplasia by screening for the level of expression of Stefin A in the myoepithelial cells. The method of the present invention is useful in a range of applications including, but not limited to, assessing a neoplastic condition, monitoring the progression of such a condition, predicting the likelihood of a subject progressing to a more advance disease state or informing decisions in relation to the design of treatment schedules.

The invention provides a tank type fermentation process for preparing low-salt chili oil thick broad-bean sauce by premixing and feeding. The tank type fermentation process comprises the following steps: putting a fermented dried chili base material and broad-bean sauce, which are obtained by tank type fermentation, into a premixing groove according to the mass ratio of (7 to 7.5) to (2.5 to 3), and feeding a mixture into a chili base material fermentation tank by adopting an underflow pump; uniformly mixing to enable the salt concentration of the material to be 10 percent to 12 percent and the moisture content to be 70 percent to 75 percent; adding saccharomycete bacterial powder into the tank; keeping constant temperature of 25 DEG C to 40 DEG C and introducing sterile air and fermenting for 4 to 5 days; then adding cooled cooked rapeseed oil into the material subjected to fermentation and dehydration treatment; heating and sterilizing to obtain a chili oil thick broad-bean sauce product. According to the method provided by the invention, the flavor and umami of a product are further improved when the requirements on quality are met; a pollution problem in a production process is avoided; meanwhile, a unique flavor of traditional Pixian thick broad-bean sauce is also kept; an occupied land is small, so that the tank type fermentation process is suitable for large-scale industrial production and the market demand of the Pixian thick broad-bean sauce is satisfied.

The invention relates to aspergillus oryzae ZA138. When the aspergillus oryzae ZA138 is applied at a koji-making stage, the caking phenomenon can be obviously reduced, the oxygen supply and growth ofaspergillus oryzae are prompted, and the activity of protease of finished koji can be obviously enhanced; the amino acid nitrogen content of soy sauce and sauce fermented by making koji using the aspergillus oryzae ZA138 is obviously enhanced, so that the product flavor is obviously improved. The invention also relates to application of aspergillus oryzae ZA138 in koji making. The invention also relates to application of aspergillus oryzae ZA138 in koji making, wherein the finished koji obtained by aspergillus oryzae ZA138 in koji making is used for preparing soy sauce and / or sauce. The invention also relates to application of the aspergillus oryzae ZA138 in preparation of soy sauce and / or sauce.

The invention provides a microbial source bactericide for preventing and treating plant diseases and a preparation method thereof, and belongs to the technical field of microbial pesticides. The preparation method of the microbial source bactericide comprises the following steps: performing slant culture on a biocontrol strain to obtain an activated strain; inoculating into a liquid culture mediumand culturing to obtain liquid seeds of each strain; transferring the liquid seeds of each strain into a solid-state fermentation culture medium containing red alga amino acid, carrying out fermentation culture, soaking in sterile water, and filtering to obtain fermentation liquor of each strain; and mixing the fermentation liquor, compounding with a carrier and an auxiliary agent, carrying out spray drying, and carrying out jet milling to obtain the microbial source bactericide. The microbial source bactericide prepared by the method has the advantages of good suspension rate, short wettingtime, strong bacteriostasis and obvious control effect on plant diseases.

The invention provides a novel assay for NS2 / 3 cleavage detection, particularly, a novel assay for the detection of the NS3 cleavage product in the presence of uncleaved NS2 / 3 based on discrimination of the activity of the resulting NS3 protease product from the NS3 protease activity of the NS2 / 3 uncleaved protein. Certain reaction conditions allow this discrimination in situ without having to resort to physical separation of both proteases by decreasing the NS3 protease activity of the NS2 / 3 protein and increasing the NS3 protease activity of the resulting NS3 protease product, thereby producing a “signal window” upon which it is feasible to distinguish and measure NS3 protease activity produced from the cleavage of NS2 / 3.

The invention provides a bacterial strain for producing low temperature alkaline protease. The bacterial strain is Bacillus sp. WFWBac-1 and is preserved at a general microbiological center of a China committee for culture collection of microorganisms of the institute of microbiology of Chinese academy of sciences located at courtyard 1, No 3, Beichen west road, Chaoyang District of Beijing on April 9, 2015, and the preservation number is CGMCC NO.10703. The filtered bacterial strain is used for producing the alkaline protease in a fermentation mode and is good in performance and high in vitality of produced protease. The carbon source of the bacterial strain is cane sugar, the nitrogen source of the bacterial strain is peptone, the initial pH value of a culture medium is 9.0, the culture temperature is 25 DEG C, and when the bacterial strain is shaken and cultured for 48 h at 150 rpm under the condition of 10% of the inoculum size, the vitality of the produced protease is as high as 995 U / mL. The prepared protease is used for preparing liquid detergents or cleanser essences.

The invention discloses applications of an aspergillus oryzae PRB-1 bacterial strain in the field of soybean sauce production. The aspergillus oryzae PRB-1 bacterial strain is obtained from soybean sauce mould starters of Guangdong PRB Bio-Tech Co., Ltd. by rejuvenation, separation and purification. The bacterial strain is accessed in the "China Center for Type Culture Collection" in December 4, 2013 with an accession number of CCTCC NO: M2013628. Many trial-produce test results show that the bacterial strain is strong in growth and reproduction capability, prosperous in mycelial growth and good in genetic stability. A finished leaven prepared by the bacterial strain is bright green in color, rich in spore, high in spore germination rate, high in protease activity, and high in output and quality of fermented soybean sauce. The bacterial strain can play an important function in making the soybean sauce and has a wide application prospect.