39results about How to "Improve breeding conditions" patented technology

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt.

The present invention discloses a method for improving growth and survival of single human embryonic stem cells. The method includes the step of obtaining a single undifferentiated HES cell; mixing the single undifferentiated cell with an extracellular matrix (ECM) to encompass the cell; and inoculating the mixture onto feeder cells with a nutrient medium in a growth environment. Therefore the single cells can survive, proliferate and grow in vitro.

The invention relates to a fruit and vegetable fiber chewable tablet with defaecation and health care functions and a preparation method of the fruit and vegetable fiber chewable tablet. A product of the fruit and vegetable fiber chewable tablet is prepared from inulin, soluble dietary fiber, oat fiber, isomalt, carrot freeze dry powder, celery freeze dry powder and magnesium stearate as raw materials according to a certain mixture ratio through steps of sieving, mixing, tabletting, internal packing and the like, and has good defaecation and health care functions. The dosage form of the fruit and vegetable fiber chewable tablet adopts the chewable tablet, the taking is convenient, the release of the effective ingredients is fast, the absorption is favorably realized, the carrying, the storage and the conveyance are convenient, the preparation method of the fruit and vegetable fiber chewable tablet is simple, the production is mechanized, the automation degree is high, the yield is high, and the cost is low.

According to the present invention, when nicotinamide is added in a culture process for producing pluripotent stem cells from human differentiated cells, it can increase the efficiency of reprogramming and can significantly reduce the time required for induction of reprogramming. It was verified that nicotinamide inhibits the induction of senescence and oxidative stress in the reprogramming process and increases cell proliferation and mitochondrial activity to effectively improve culture conditions for induction of reprogramming. Particularly, the present invention will contribute to optimizing a process of producing induced pluripotent stem cells from a small amount of patient-specific somatic cells obtained from various sources, and thus it will significantly improve a process of developing clinically applicable personalized stem cell therapy agents and new drugs and will facilitate the practical application of these agents and drugs. In another aspect, according to the present invention, in defined culture conditions in which feeder cells and serum were not used, it was found that nicotinamide can provide a culture medium composition effective for maintaining the undifferentiated state of human embryonic stem cells and human induced pluripotent stem cells, which are typical pluripotent stem cells. The invention can be effectively used for the development of a high-efficiency system for culturing large amounts of human pluripotent stem cells, which is required for the industrialization of human pluripotent stem cells.

The invention discloses a Chinese black sleeper culture method and solves the problems that Chinese black sleepers grow slowly and feed waste is apt to caused by adopting the existing Chinese black sleeper culture method in the prior art. The Chinese black sleeper culture method comprises the following steps: 1) fishpond selection; 2) fishpond dredging and water fertilization; 3) fry putting-in; 4) culture. The Chinese black sleeper culture method has the advantages that the steps are simple, the operability is high and the conditions for Chinese black sleeper culture are optimized; since different feeding periods and feeding amounts are adopted by using a biological compensation mechanism aiming at fish fries with different body length and optimized mixed feed is used, the growth of the Chinese black sleepers is effectively promoted, the culture period is shortened, the feed utilization ratio and the survival rate are improved and the culture benefits are improved.

The present invention pertains to a method for culturing a suspension of immortalized human blood cells, preferably cells of myeloid leukaemia origin or cells derived therefrom, wherein said method provides a high productivity, a high cell viability and growth rate and a high batch-to-batch consistency, and can be scaled up without altering these parameters.

The invention relates to the technical field of fish culture, in particular to an artificial breeding method of lateolabrax japonicus in northern water areas. The method comprises parent fish selection, parent fish rearing, artificial spawning inducement and insemination and fry rearing. Wild lateolabrax japonicus fries are domesticated and cultured into adult fishes, and high-quality parent fishes are stored by parent fish domestication and screening to become fine breeds for industrialization of the lateolabrax japonicus. Artificially bred fries of the lateolabrax japonicus are provided forthe northern water areas, the limitation of traditional natural conditions is broken through, the natural resources are protected, and the ecological balance is kept.

The invention discloses a method for remediating soil by using thermal desorption and a microbial technology. The soil is remediated by utilizing a thermal desorption soil remediating system, and the method comprises the following steps: firstly, preheating contaminated soil by adopting a preheating device, then, heating the preheated contaminated soil by adopting a heating device to separate contaminants in the soil, and finally, recovering heat of the soil from which the contaminants are separated by adopting a heat recovery device; remediating the soil by utilizing the microbial technology; applying organic fertilizer to the soil treated by thermal desorption per mu, and plowing the organic fertilizer under the soil by a rotary cultivator; then, sprinkling the soil to keep soil humidity, and then, evenly spraying microbial soil remediating inoculant; and evenly spraying the microbial soil remediating inoculant once after two months to finish soil remediation after three months. According to the method disclosed by the invention, by adopting thermal desorption and the microbial technology, a breeding condition in favor of microbial strains is created by utilizing a physical and biological method, and the soil is remediated in manner of nature, return and equilibrium.

The invention discloses a method to interplant nature plants in the desert sand land. It comprises the following steps: plant the artificial plants in the form of strip or net where need to plant; set the agricultural macromolecular absorbent resin into the earth where the artificial plant root is when the artificial plants are being planted; plant the nature plants among the gap of the strip-shape or net-shape areas where the artificial plants are to form the mixed forest or grassland; after the nature plants' surviving, move the artificial plants and plant the nature plants where the artificial plants have been moved away. The invention has many merits and positive effects as following: plant man-made forest in large areas with machines at a high speed, prevent huge sand blown by wind in desert areas, reduce the water evaporating and the lost of water and soil. Plant economic plants in the gap of man-made plant can improve the rate of surviving effectively.

The invention relates to an environment-friendly microbial fermentation non-polluted liquid-state feed and a production method thereof. At present, there exist a lot of technologies and antibiotic substitutes concerning non-antibiotic feeding of livestock and poultry at home, however, the overwhelming majority of the technologies and substitutes are hard to achieve commercialized promotion.The environment-friendly microbial fermentation non-polluted liquid-state feed comprises the following components according to parts by weight: 65-77 parts of energy feed raw material, 22.5-29.5 parts of protein feed raw material, 1.2-1.4 parts of mineral substance, 0.163-0.178 part of vitamin, 0.1-0.15 part of amino acid, 11 parts of probiotic flora and 26 parts of complex enzyme preparation.The product of the invention is applied to feeding of live pigs, involving all phases of reproduction and flattening.

The invention discloses a preparation method of a biofilter packing and a biological aerated filter. The preparation method comprises the following steps: adding Fe3O4 powder and activated carbon powder into an aqueous solution of a silane coupling agent, and carrying out ultrasonic treatment and stirring so as to prepare a suspension; adding carboxymethyl cellulose into the suspension to prepareslurry, and then drying and grinding the slurry to prepare composite powder; weighing 30-40 parts by weight of volcanic particles, 20-30 parts by weight of fly ash, 30-40 parts by weight of clay, 4-8parts by weight of a pore forming agent and 35-50 parts by weight of the composite powder, uniformly mixing, adding 25-35 parts by weight of water, and granulating to prepare particles; preheating theparticles to 105-115 DEG C, roasting at 500-700 DEG C, cooling and magnetizing to prepare the biofilter packing. The method is simple in process, and the prepared biofilter packing is high in ammonia-nitrogen removal rate and strong in decontamination capability, and can ensure better mechanical strength.

The invention provides a blended fertilizer. The blended fertilizer is composed of urea, ammonium dihydrogen phosphate, ammonium sulfate, potassium sulfate, potassium nitrate, boric acid, zinc sodium tetraacetate and a flavoring bag, wherein the flavoring bag comprises a chelating agent, a complex enzyme, fulvic acid and seaweed extract. Every 1500 kg of the finished blended fertilizer is composed of 162.5 kg of urea, 296 kg of ammonium dihydrogen phosphate, 100 kg of ammonium sulfate, 60 kg of potassium sulfate, 366 kg of potassium nitrate, 9 kg of boric acid, 3.5 kg of zinc sodium tetraacetate, 50 kg of the chelating agent, 100 kg of the complex enzyme, 273 kg of fulvic acid and 80 kg of seaweed extract. The blended fertilizer provided by invention adopts three major ecological chain technologies and utilizes organic carbon for chelation with residual salt in soil, so the root systems of crops are well developed, the quality and appearance of fruit are improved, the purposes of increasing yield and increasing the income of farmers are achieved, and the problems of soil acidification, soil hardening, soil-borne diseases and the like are overcome. Thus, the blended fertilizer has good promotion value.

The invention discloses a composition containing eaglewood leaves and having the efficacy of loosening bowel to relieve constipation. The composition is prepared from 1-40 parts of eaglewood leaves, 5-10 parts of balsampear leaves, 10-25 parts of freeze-dried celery powder, 10-50 parts of wheat bran dietary fibers, and 10-50 parts of oat dietary fibers. The composition disclosed by the invention has a good effect of relaxing bowel to relieve constipation, can treat and prevent constipation, besides, can improve the reproduction conditions of beneficial bacteria in a human body, can restrain the growth of saprophytic bacteria, and can maintain vitamin supply, so that the composition is a health product which is safe and is simple and convenient to take.

An underwater aquaculture box comprises a box body and a box cover. The box body is of a cubic or cylindrical structure, the box cover is mounted at the top of the box body in a clamped manner, a through hole is formed in the surface of the box cover, a feed pipe is mounted at the position of the through hole, and a filter screen is arranged at the position of the through hole; the feed pipe is vertically mounted; a horizontal partition is arranged in the box body to have the same partitioned into an upper portion and a lower portion, the upper box body is a storage box, and the lower box body is a feeding box; filter holes are arranged in the surface of the horizontal partition; openings are formed in two sides of the lower box body, water incoming pipes of reducing-tube structures are mounted in the positions of the openings, and a protecting net is arranged at the position of the smaller orifice of each water incoming pipe. The underwater aquaculture box has the advantages of ingenious structure, convenience in use and the like.

A method for culturing the tomato resisting against rhizomynematoda features the grafting between the tomato Energy (Lesuclentum * Lesculentum) as stock with high resistance to rhizomynematoda and the tomato FA-189 as scion with high sensitivity to rhizomynematoda.

The invention relates to a method for cultivating a root-knot nematode-resistant eggplant plant. The method is to graft a plant of a conventionally cultivated highly root-knot nematode-sensitive eggplant variety serving as a scion onto a stramonium plant serving as a stock to obtain a graft plant, that is, the root-knot nematode-resistant eggplant plant. The root-knot nematode-resistant stramonium plant is adopted as the stock, the plant of the conventionally cultivated highly root-knot nematode-sensitive eggplant variety is adopted as the scion and the seedlings are grafted at the most proper position by the most proper method in a period of highest affinity, so the method can improve the inosculation of the two, greatly improve the survival rate of grafting by improving cultivation conditions. The grafted seedling has obvious prevention and cure effect on root-knot nematode and overcomes a continuous cropping obstacle, so the method enormously improves economic efficiency and, meanwhile, achieves obvious ecological benefits due to reduction in use of agricultural chemicals.

The invention relates to bacillus velezensis MVCR2 which can improve the immunity of rainbow trout and has a remarkable inhibition effect on aeromonas salmonicida and vibrio anguillarum. The invention further relates to a compound bacterium preparation containing the bacillus velezensis MVCR2. The invention further relates to application of the bacillus velezensis MVCR2. The bacillus velezensis MVCR2 disclosed by the invention is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M 2021507, and the preservation date is May 8, 2021, and the preservation number is CCTCC NO: M 2021507. The bacillus velezensis MVCR2 has a remarkable inhibition effect on aeromonas salmonicida and vibrio anguillarum, has an effective prevention and treatment effect on various aquaculture diseases caused by the aeromonas salmonicida and the vibrio anguillarum, and can improve the growth and immunity of rainbow trout, increase the survival rate of rainbow trout and reduce the death rate of freshwater rainbow trout in the sea.

The invention provides a plant floating bed. The plant floating bed mainly comprises a supporting frame, a floating body which is mounted at the bottom of the supporting frame and a carrier on the upper surface of the supporting frame; the supporting frame forms a plane by splicing and constructing bracket monomers; the supporting frame floats on the surface of water by the floating body; the carrier comprises a supporting net on the supporting frame and soil and plant humus which are spread on the supporting net; two or more aquatic plants are planted on the carrier in a mixed manner; the aquatic plants absorb nutrient elements, such as nitrogen and phosphorus, in a water body, and the eutrophication degree of the water body is lightened, so that an effect of purifying water quality is achieved; the invention also provides a method for treating water body pollution by the plant floating bed.

A method for culturing the tomato resisting against rhizomynematoda features the grafting between the tomato He-Man (L.lycopersicum * L.hircutum) as stock with high resistance to rhizomynematoda and the tomato FA-189 as scion with high sensitivity to rhizomynematoda.

The invention relates to a photobioreactor for cultivating photosynthetic micro-organisms, comprising: a) at least one cultivation container (1) for containing the culture medium (3) of the micro-organisms, b) photovoltaic cells (2) isolated from the culture medium (3), emitting light towards the culture medium (3), and c) means (4) for powering the photovoltaic cells (2) in order to operate the photovoltaic cells in light emission mode.

The invention discloses a preparation method of an antibacterial peptide gynecologic washing lotion. The preparation method comprises the following steps: (1) culturing bees in an environment containing gardnrella vaginallis and bacteroides fragilis, heating and drying bees, adding licorice tablets, and grinding bees to obtain bee powder; (2) adding bee powder into a glycerin-acetic acid solutioncontaining glucose, soaking, and then carrying out ultrasonic vibration to obtain a mixed solution; (3) filtering the mixed solution, removing the filter residues, and dry-freezing the filtrate to obtain a bee extract; and (4) adding the bee extract and vitamin C into cucumber juice, stirring, then adding a MgSO4 solution, and stirring to obtain the washing lotion. The prepared antibacterial peptide gynecologic washing lotion can be absorbed by the patients peacefully, the pathogenic bacteria are killed, and at the same time, a comfortable and healthy environment is created for the growth andproliferation of beneficial bacteria.

The invention relates to a method for cultivating a root-knot nematode-resistant eggplant plant. The method is to graft a plant of a conventionally cultivated highly root-knot nematode-sensitive eggplant variety serving as a scion onto a stramonium plant serving as a stock to obtain a graft plant, that is, the root-knot nematode-resistant eggplant plant. The root-knot nematode-resistant stramonium plant is adopted as the stock, the plant of the conventionally cultivated highly root-knot nematode-sensitive eggplant variety is adopted as the scion and the seedlings are grafted at the most proper position by the most proper method in a period of highest affinity, so the method can improve the inosculation of the two, greatly improve the survival rate of grafting by improving cultivation conditions. The grafted seedling has obvious prevention and cure effect on root-knot nematode and overcomes a continuous cropping obstacle, so the method enormously improves economic efficiency and, meanwhile, achieves obvious ecological benefits due to reduction in use of agricultural chemicals.

The invention relates to a photobioreactor for cultivating photosynthetic micro-organisms, comprising: a) at least one cultivation container (1) for containing the culture medium (3) of the micro-organisms, b) photovoltaic cells (2) isolated from the culture medium (3), emitting light towards the culture medium (3), and c) means (4) for powering the photovoltaic cells (2) in order to operate the photovoltaic cells in light emission mode.

The invention relates to a preparation method of an organic compound trace element for laying fowl. The product contains an additive and a carrier. In the formula, raw materials comprise (by weight):0.5-6% of flour, 10-35% of corn protein powder, 1-10% of squid visceral ointmen, 0-3% of calcium dihydrogen phosphate, 0-2.5% of soybean phospholipid oil, 5-20% of peanut meal, 10-20% of a DDGS feed and 30-60% fish oil. The product prepared by this method is a novel environmentally-friendly, nutritious and efficient laying fowl feed which helps reduce diseases of laying fowl, improve breeding conditions and increase profit.

The invention discloses a preparation method of a biological filter filler and a biological aerated filter. The preparation method comprises: adding Fe 3 o 4 Powder and activated carbon powder are added to the aqueous solution of silane coupling agent, ultrasonically and stirred to make a suspension; carboxymethyl cellulose is added to the suspension to make a slurry, then dried and ground to make a composite powder; Weigh 30-40 parts by weight of volcanic rock particles, 20-30 parts by weight of fly ash, 30-40 parts by weight of clay, 4-8 parts by weight of pore-forming agent, and 35-50 parts by weight of the composite powder, mix well, add water 25-35 parts by weight, granulated to make granules; the granules are preheated to 105-115° C., then roasted at 500-700° C., cooled, and magnetized to make biofilter fillers. The method has simple process, and the prepared biofilter filler has high ammonia nitrogen removal rate, strong decontamination ability and better mechanical strength.

The invention discloses an energy-saving water feeder for sow breeding in a livestock farm, and relates to the field of sow breeding. The energy-saving water feeder comprises a fixing frame, the top of the fixing frame is provided with a water storage tank, the inner wall of the water storage tank is provided with a temperature sensor, the side, away from the temperature sensor, of the inner wall of the water storage tank is provided with a liquid level sensor, a heater is arranged in the middle of the bottom of the water storage tank, a controller is installed on the side wall of the water storage tank, a water conveying pipe is arranged at the bottom of the water storage tank, a duckbilled water fountain is arranged at one end of the water conveying pipe, and a collecting frame is arranged at the top of the fixing frame. according to the energy-saving water feeder, water is filtered through a water purification cylinder and then conveyed into the water storage tank through a water outlet pipe, the controller enables the heater to work to heat drinking water, the water in the water storage tank is kept at a constant temperature, sows drink warm water in winter, the disease probability of the sows is reduced, the water is fed through the relatively independent water feeder, better breeding conditions are provided for the sows at different stages, and the breeding cost of the sows is reduced.

The present disclosure relates to a method for improving the culture conditions of cultures of primary sweat gland cells and / or three-dimensional sweat gland equivalents, where the culture with from about 500 to about 500,000 sweat gland cells and a diameter of from about 100 to about 6,000 nm is initially prepared and then cultivated in a culture medium with neurotransmitters and / or from about 11 to about 100 ng / ml of at least one growth factor. Cultivation of these cells and models in a culture medium containing at least one neurotransmitter and / or from about 11 to about 100 ng / ml of at least one growth factor leads to an increase of sweat-gland-specific marker proteins. As a result, a loss of function, which occurs with an absence of or only low expression of these markers, can be avoided.