Preparation method for organic material decomposition agent

A technology of organic materials and decomposing agents, applied in the direction of organic fertilizers, etc., can solve the problems of poor adaptability of strains, low degree of industrialization, and unstable effects, and achieve the effects of stimulating growth and development, high spore count content, and advanced and scientific preparation methods

Inactive Publication Date: 2013-11-13
SICHUAN WO DA BIOLOGICAL TECH
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing an organic material decomposing agent, which solves the problems in the prior art that there are single strains, few types, poor strain adaptability, unstable effect, and low degree of industrialization.

Method used

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  • Preparation method for organic material decomposition agent
  • Preparation method for organic material decomposition agent
  • Preparation method for organic material decomposition agent

Examples

Experimental program
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Embodiment

[0037] Preparation of Bacillus subtilis:

[0038] (1) Inoculate the prepared eggplant-shaped bottle with the Bacillus subtilis strains preserved in the laboratory in a sterile environment, cultivate it at 31-32°C for 31-35 hours, check for no bacteria and keep it as a fermenter vaccination;

[0039] (2) Preparation of fermentation medium: The medium materials in the fermentation tank are prepared according to the ratio of 20g of glucose, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract and 20g of agar per liter of water, so that they are dissolved in water and sent to into the fermenter;

[0040] (3) Inoculation: Inoculate several bacteria-free eggplant-shaped bottles obtained in the process of step (1), and inoculate 3 eggplant-shaped bottle strains per cubic medium, and insert them into the sterilized fermenter through aseptic operation middle;

[0041] (4) Fermentation culture: Stir and cultivate the fermenter at a temperature of 29-34°C and a rotation speed o...

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Abstract

The invention discloses a preparation method for an organic material decomposition agent to solve problems comprising strain unicity, less types, bad strain adaptability, unstable effects and a low degree of industrialization in the prior art. According to the method, bacillus subtilis, aspergillus oryzae and brewer's yeast are selected and used. After steps of preparing nutrition mediums, culturing individually and controlling inoculation quantities, fermentation temperatures, fermentation times and adjusting pH parameters, concentrating and drying, and adjusting ratios to prepare the decomposition agent capable of making crop straws return to the field in situ. According to the method, the bacillus subtilis, the aspergillus oryzae and the brewer's yeast are selected. The three stains have good symbiosis and metabiosis effects and have no antagonistic effect, thereby enhancing protease activity of products and capability of antagonizing crop pathogenic bacteria, and playing functions of bio-control and plant growth promotion.

Description

technical field [0001] The invention relates to a preparation method of an organic material decomposing agent. Background technique [0002] The annual output of various straws in our country is as high as more than 50 million tons. In order to deal with these remaining straws, many farmers directly burn the straws produced in the current season in the fields. Straw burning not only destroys the physical and chemical properties of the soil, but also seriously wastes resources, pollutes the environment, and destroys the ecology. In order to fundamentally solve this contradiction, my country has carried out research on "returning crop straws to the field to increase the content of soil organic matter". Improve the organic matter content of the soil and realize the recycling of straw resources. [0003] The technical principle of the existing straw decomposing agent is to screen out microbial strains that produce "three enzymes": cellulase and hemicellulase according to the ma...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 胡白雨
Owner SICHUAN WO DA BIOLOGICAL TECH
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