7results about How to "No antagonistic effect" patented technology

The application discloses application of gynostemma pentaphyllum saponin H combined with azole drugs in preparation of antifungal drugs and the antifungal drugs, and relates to the technical field of antifungal drugs. It is found through systematic in-vitro drug sensitivity test that natural product gynostemma pentaphyllum saponin H combined with specific azole antifungal drugs (especially posaconazole POS) can produce significant synergistic antifungal effect, especially for clinical thorny aspergillus, candida, new cryptococcus and dark fungi. The combination strategy can significantly reduce the minimum inhibitory concentration of azole drugs, reverse drug resistance, and is effective for multi-drug resistant strains (such as candida auris). The application provides an efficient and low-toxicity new combination drug scheme for overcoming the increasingly serious fungal drug resistance problem.

This invention discloses a fermentation agent for treating agricultural ecological solid waste, its preparation method, and its application, belonging to the field of microbial technology. The fermentation agent of this invention comprises compound functional bacteria, soybean meal, molasses, disodium hydrogen phosphate, corn straw powder, and a preservative. The compound functional bacteria include *Bacillus subtilis*, *Trichoderma helicola*, and *Bacillus bellis*. This invention uses a compound of three strains—*Bacillus subtilis*, *Trichoderma helicola*, and *Bacillus bellis*—in a specific ratio to synergistically accelerate the decomposition of straw, effectively shortening the fermentation cycle and improving the decomposition effect. This agent can efficiently degrade agricultural ecological solid waste, converting it into high-quality organic fertilizer, thus turning waste into treasure. It solves the problem of agricultural solid waste pollution and saves fertilizer resources, demonstrating significant ecological, economic, and social benefits.

This invention belongs to the field of environmental microbiology, specifically a type of Klebsiella variicola and its applications. Klebsiella variicola, strain IFPBD3, is deposited at the China General Microbiological Culture Collection Center (CGMCC) on May 25, 2023, with accession number CGMCC No. 27461. The rice straw degrading bacteria produced by the technical solution of this invention can effectively degrade rice straw, significantly increase straw fertility, and improve straw utilization rate.

This invention discloses a method for preparing a water-retaining and retarding polycarboxylate superplasticizer, belonging to the field of concrete admixture technology. The method includes: using pentenol polyoxyethylene ether and / or methyl allyl polyoxyethylene ether, hydroxyethyl acrylate, hydrogen peroxide, vitamin C, mercaptopropionic acid, acrylic acid, and phosphorus-containing compounds as raw materials, a polycarboxylate superplasticizer is prepared via free radical polymerization; then, hydroxyl and carboxyl compounds are added sequentially for slow modification; after adjusting the system to a strongly alkaline state, refined cotton powder, chloromethane, and propylene oxide are added, and an etherification and crosslinking reaction is carried out under pressure and heating conditions; finally, the product is obtained by pressure filtration, pulverization, and drying. The polycarboxylate superplasticizer prepared by this invention possesses excellent water retention and retarding effects, effectively reducing concrete bleeding and segregation, extending setting time, improving concrete workability and durability, and exhibiting good compatibility with cement, making it suitable for high-temperature construction, large-volume concrete, and manufactured sand concrete projects.

This invention discloses a nutrient-degrading enzyme, engineered microbial agents, and their application in the nutrient regulation of polymeric slow-release fertilizers, belonging to the field of enzyme engineering technology. This invention utilizes a specific polymeric slow-release fertilizer-degrading enzyme modified by artificial intelligence, which not only retains the degradation activity of the source strain enzyme but also achieves efficient heterologous expression while promoting a dual improvement in stability and catalytic efficiency. The constructed composite engineered strain exhibits no antagonistic effect, enabling low-cost, large-scale production of the degrading enzyme. The composite engineered strain can achieve precise regulation of nutrient release from polymeric slow-release fertilizers. By adjusting the ratio of engineered strains producing peptidases and amidases, precise supply of nutrients to different stages throughout the growth cycle of different crops can be achieved, resulting in complete degradation of polymeric slow-release fertilizers, significantly improving crop yield and nutrient utilization, and showing significant application prospects in the development of precision agriculture.

The invention relates to the technical field of microorganisms, and discloses propylene glycol eating lactobacillus and application thereof in a combined leavening agent and koumiss, the propylene glycol eating lactobacillus MNJ-2 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.35857, the preservation number is CGMCC No.35857, and the preservation number is CGMCC No.35857. The invention relates to a lactobacillus plantarum strain, which has good acid resistance, cholate resistance and intestinal epithelial cell adhesion capacity, shows the potential of serving as a probiotic and has no obvious antagonism with other strains, and further relates to a leavening agent prepared by combining the strain with lactobacillus plantarum ZKLp l 800, lactobacillus paracasei ZKL200 and saccharomyces cerevisiae ZKM011 in proportion. Compared with traditional koumiss, the koumiss produced by applying the combined leavening agent is more stable in state, richer in fragrance and richer in taste, meanwhile, the traditional sour taste and wine fragrance are reserved, and the koumiss has wide application prospects in the aspect of industrial production.

The invention discloses a seed treatment agent for preventing and treating garlic bulb rot, and belongs to the technical field of agricultural biology. The active ingredients of the seed treatment agent comprise metalaxyl-M, fludioxonil and clothianidin. Metalaxyl-M and fludioxonil are compounded to respectively show extremely high inhibitory activity on two main pathogenic bacteria, namely phytophthora and fusarium, causing garlic bulb rot, and after compounding, antagonism is avoided, so that the metalaxyl-M and fludioxonil can synergistically exert prevention and treatment effects. The treatment agent can be prepared into dosage forms such as a suspended seed coating and the like, and is used for seed dressing or coating treatment of garlic seeds. Field experiments show that the seedling death rate and the bulb rotting incidence rate can be remarkably reduced, yield increase is promoted, and the method is safe to seeds. The invention fills the blank of the special prevention and treatment medicament for garlic bulb rot, and has important application value.