50results about How to "Improve synergistic antibacterial effect" patented technology

The invention discloses a nanometer antibacterial agent loaded with both nano-silver and curcumin and a preparing method and application thereof. A polymeric micelle formed through self-assembly of the amphiphilic block copolymer PCL-b-PAsp is taken as a nano-carrier, positively charged Ag+ ions are adsorbed by means of the electronegativity of a PAsp chain segment in a hydrophilic shell and converted into nano-silver through in-situ reduction by means of a reductant, the natural and nontoxic antibacterial constituent curcumin is coated with hydrophobic core PCL, and in this way, the polymeric micelle loaded with both nano-silver and curcumin is formed and can be used for improving antibacterial activity. The nanometer antibacterial agent has high synergetic antibacterial efficiency for pseudomonas aeruginosa and staphylococcus aureus. The preparing method is simple, and raw materials have high biocompatibility and biodegradability, are cheap and easy to obtain and can be converted towards the clinical direction easily. Compared with a nanometer antibacterial agent loaded with either nano-silver or curcumin, the nanometer antibacterial agent loaded with both nano-silver and curcumin has the advantage that the synergetic pathogenic bacteria killing effect is good, and the anometer antibacterial agent loaded with both nano-silver and curcumin has broad application prospects.

The invention provides a microcapsule containing a plant characteristic aroma and a preparation method of the microcapsule. The microcapsule is prepared from, by weight, 3-7 parts of fever-trees, 15-25 parts of orange peel, 1-5 parts of thyme, 1-4 parts of vanilla, 3-6 parts of cortex cinnamomi and 10-20 parts of lavandula angustifolia. The microcapsule has the advantages that various materials have mutual synergistic effects, synergize with one other and mutually enhance the antibacterial properties, thereby enhancing the antibacterial property of the microcapsules; by adding plant extracts such as the orange peel and the lavandula angustifolia, the aroma of the microcapsule is enriched, and the effective time of air freshening is greatly prolonged.

The invention relates to an artificial tooth root with a synergistic antibacterial coating and a preparation method of the artificial tooth root. The artificial tooth root with the synergistic antibacterial coating is composed of a titanium or titanium alloy artificial tooth root matrix and a lamination nanostructure TiO2 coating loaded with silver nanoparticles (AgNPs) and graphite like carbon nitride (g-C3N4) on the surface. The preparation method includes the steps that firstly, rod-shaped nanostructure sodium titanate is prepared on the surface of titanium or a titanium alloy by a hydrothermal synthesis technology; secondly, the AgNPs is loaded on the surface of the rod-shaped nanostructure sodium titanate by an ion exchange technology and an ultraviolet light reduction method; thirdly, the silver loaded sodium titanate is transformed into the TiO2 bioactive coating loaded with the AgNPs on the surface by a high-temperature annealing process; and finally, a chemical vapor deposition technology is used for depositing g-C3N4, and the surface of the TiO2 bioactive coating is transformed into lamination nanostructure TiO2 coating with g-C3N4/Ag, therefore, the artificial tooth rootwith the synergistic antibacterial coating is obtained.

The invention discloses a new uses of helvolicacid sterides in making drugs of antibiotic-resistant microbes, including helvolicacid, fusidic acid and its salt and salt hydrates acceptable in pharmacology, compound of ester or prodrug with single or multi-antibiotic, applying to preparation of drugs of antibiotic-resistant microbes.

The invention belongs to the field of biotechnology and particularly relates to pichia pastoris expressed recombinant chicken Cathelicidin antibacterial peptide and further discloses a preparation method and application thereof. In the method for preparing recombinant chicken Cathelicidin antibacterial peptide disclosed by the invention, a pPICZalphaA secreted expression vector is selected as a eukaryotic expression system of the exogenous gene so that the recombinant chicken Cathelicidin antibacterial peptide is efficiently expressed and direct killing of host cells caused by the expression product is avoided. Meanwhile, the method has the advantages of high expression efficiency, simple separation and purification of expression products, low production cost, easiness in magnification and good stability and is suitable for large-scale industrial production. According to experimental verification, the combination of Cathelicidin antibacterial peptide and antibiotic realizes an obvious synergistic antibacterial effect on multiple kinds of gram-positive bacteria and gram-negative bacteria, and the application and promotion prospects are broad.

The invention relates to the field of food safety, and particularly discloses a mixed preparation with bacteriophages LPEE17 and LPEK22 as major components and application. The two coliphages LPEE17 and LPEK22 are provided, and both the coliphages are virulent bacteriophages and can be used for lysing colibacillus with drug resistance, wherein the LPEE17 can lyse the colibacillus O157:H7, and theLPEK22 can lyse the colibacillus O157:H7 and also can lyse serotype salmonella typhimurium and serotype salmonella enteritidis. The antibacterial effects of the LPEE17 and LPEK22 on the colibacillus EDL933 in milk and ham sausages under single use and mixed use conditions are compared, the mixed bacteriophages have a better antibacterial effect in the foods, and a significant synergistic effect isachieved.

The invention discloses an antibacterial type nano-fiber-based fresh-keeping film which is composed of the following raw materials by weight: 1-2 parts of sodium tripolyphosphate, 130-150 parts of polyethylene, 1-2 parts of mannan, 10-14 parts of nano crystalline cellulose, 4-7 parts of cellulose acetate propionate, 10-14 parts of methyl ester nylon acid, 1-2 parts of 8-hydroxyquinoline, 0.7-1 part of Diisopropylethanolamine, 5-8 parts of Triethylene Glycol Di-2-ethylhexoate, 3-4 parts of sucrose acetate isobutyrate, 5-7 parts of allylpolyethyleneglycol, 10-20 parts of diatomite, 40-50 parts of a bacteriostatic water dispersion and 2-3 parts of Hydroxyethylidene-1,1-diphosphonic acid, after the added diatomite is modified by esterification, the diatomite is well compatible with a polymer matrix, and effectively improves the stability strength of a finished film, and the antibacterial type nano-fiber-based fresh-keeping film has good toughness, good antibacterial activity, good use safety and excellent comprehensive performance.

The invention discloses a polylactic acid material with anti-adhesion and sterilization functions, which is prepared by the following steps: 1) stirring and dissolving a biodegradable material polylactic acid in a good solvent, adding a poor solvent to realize phase separation, and fully stirring to obtain a coating solution; 2) uniformly coating a polylactic acid sheet with the solution obtained in the step 1), and drying; 3) stripping the top layer polylactic acid by using an adhesive tape to obtain a super-hydrophobic surface; and 4) dispersing chlorophyll in absolute ethyl alcohol, coating on the super-hydrophobic surface of polylactic acid, and drying. The surface of the material has the super-hydrophobic performance of resisting initial adhesion of bacteria, and the adhered bacteria can be killed through photodynamic force. A super-hydrophobic and photodynamic synergistic antibacterial mode is adopted, the dosage of a bactericide is small, the sterilization efficiency is high, the antibacterial long-term effect is good, the controllability is high, and bacteria cannot be induced to generate drug resistance. The polylactic acid material with anti-adhesion and sterilization functions prepared by the invention is non-toxic and harmless, has good biological and environmental compatibility, and can be used in the aspect of bacterial infection resistance of medical or packaging materials.

The invention provides a sulbactam sodium composition freeze-dried powder for injection, and belongs to the field of medicine and medicine preparation technology. The sulbactam sodium composition freeze-dried powder comprises following raw material ingredients, by weight, 7.26 to 9.17 parts of sulbactam sodium, 6.02 to 7.97 parts of chitosan nanoparticle, and 81.34 to 87.55 parts of injection water. Advantages of the sulbactam sodium composition freeze-dried powder are that: 1) the sulbactam sodium composition freeze-dried powder possesses excellent synergistic antimicrobial effect with sulbactam sodium, antibiotic sensitive rate of various bacteria on the sulbactam sodium composition freeze-dried powder is high; 2) stability of sulbactam sodium against beta-lactamase is increased, and in vivo distribution is excellent; 3) improvement of activity is capable of shortening medication cycle of patients, and reducing occurrence likelihood of adverse reaction caused by accumulation of sulbactam sodium; and 4) the chitosan nanoparticle can be used as a freeze-dried skeleton agent of the freeze-dried powder injection instead of mannitol so as to avoid active effects of mannitol on human bodies.

The invention belongs to the biotechnology field, particularly relates to a pichia pastoris-expressed recombinant chicken antibacterial peptide Cathelicidin 2, and further discloses a preparation method and application thereof.According to the preparation method of the recombinant chicken antibacterial peptide Cathelicidin 2, pPICZ alpha A secreting type expression vector is selected as an eukaryotic expression system of exogenous genes, the recombinant chicken antibacterial peptide Cathelicidin 2 is effectively expressed, and direct destruction caused by an expression product on host cells is avoided.Meanwhile, the method is high expression efficiency, the expression product is easy to separate and purify, the production cost is low, enlarging is easy, stability is good, and the method is suitable for large-scale industrial production.Experiments prove that the antibacterial peptide Cathelicidin 2 has the obvious synergistic antibacterial effect when used in cooperation with antibiotics, and has the wide application and popularization prospect.

The invention relates to the technical field of high molecular materials and relates to a high-antibacterial and wear-resisting PVC (Polyvinyl Chloride) resin material and a preparation method thereof. The high-antibacterial and wear-resisting PVC resin material is prepared from the following components in parts by weight: 100 parts of PVC resin, 10 to 40 parts of an antibacterial plasticizer, 8 to 12 parts of a stabilizer, 1 to 3 parts of a lubricant, 5 to 8 parts of an antioxidant, 3 to 5 parts of an anti-impact modifying agent, 5 to 20 parts of filler and 1 to 3 parts of an additive. According to the high-antibacterial and wear-resisting PVC resin material and the preparation method thereof, an antibacterial functional auxiliary agent and a plasticizing-promoting auxiliary agent are integrated, have a good plasticizing effect and a relatively strong antibacterial effect, and are matched with other components to have a mutual synergistic effect, so that the PVC material has a good antibacterial effect and good mechanical properties and weather-resisting performance; the application field and application field of PVC are greatly improved; furthermore, the preparation method provided by the invention is simple, preparation technological conditions are easy to control and the preparation efficiency is high; the raw material price and the production energy consumption are relatively low, so that the preparation method is suitable for large-scale production.

The invention discloses a method for prolonging shelf life of a bread product so as to solve the problems that conventional bread products are easy to pollute by moulds and short in shelf life. According to the method disclosed by the invention, epsilon-polylysine and natamycin are used as fresh keeping materials to prepare an antiseptic fresh keeping agent having the effect of synergistically resisting bacteria, then the antiseptic fresh keeping agent is applied to the surface of bread in a spraying manner, the bread is placed at room temperature under sterile environment for natural airing,and then sealing and packaging are performed. Through adoption of the method disclosed by the invention, the operation is extremely simple and convenient, the fresh keeping effect is good, the methodis healthy, safe and harmless to human bodies, is low in cost and is suitable for enterprises to popularize and apply; satisfactory antiseptic fresh keeping effects are provided; and besides, lysine necessary for human bodies can also be provided.

The invention discloses a method for inactivating Listeria monocytogenes in food by cooperating ultrahigh pressure with an antibacterial agent. The method comprises the following steps of (1) LB1 andLB2 liquid culture medium preparation; (2) Listeria monocytogenes inoculating and activating, wherein the Listeria monocytogenes is inoculated into an LB1 liquid culture medium for culture through a slant culture medium to obtain an LB1 bacterial solution, and the LB1 bacterial solution is taken and added into an LB2 liquid culture medium to obtain an LB2 bacterial solution; (3) ultrahigh pressuretreatment, wherein the antibacterial agent is added into a Listeria monocytogenes solution separately to obtain a bacterium suspension of Listeria monocytogenes, vacuum sterile sealing is conducted on the suspension, and ultrahigh pressure treatment is conducted; (4) counting, wherein the Listeria monocytogenes obtained after ultrahigh pressure treatment is cultured and counted. Accordingly, theinactivation effect is greatly improved, the sublethal damage rate is as low as 15.24%, and the lethal rate is up to 100%; the control over the sublethal state of the Listeria monocytogenes is achieved, and the treatment pressure can be reduced.

The invention discloses nano antibacterial bone filling materials and a production method thereof. The bone filling materials comprise hydroxyapatite, nano silver powder, nano titanium dioxide, glycerol, polyvinyl acetate, lemongrass extract, nano silicon dioxide and epoxy resin. The production method includes: mixing the hydroxyapatite, the nano silver powder, the nano titanium dioxide, the nano silicon dioxide and the epoxy resin, then grinding into 800-mesh powder, adding into a reaction kettle, heating at a constant speed to 2000-2500 DEG C, keeping the temperature for 5-10 minutes, and heating to 2800 DEG C to obtain first mixed liquid; adding the glycerol and the lemongrass extract when the first mixed liquid is cooled to 40-50 DEG C, and evenly stirring to obtain the nano antibacterial bone filling materials. The nano antibacterial bone filling materials are high in porosity, good in antibacterial effect, and capable of accelerating bone healing and reducing the pain of patients.

The invention relates to a processing method of fishes. The method comprises the following steps: (1) carrying out rough processing treatment on raw material fishes; and (2) carrying out fresh-keepingtreatment on the raw material fishes after the rough processing treatment. The fresh-keeping treatment specifically comprises the following steps: firstly, treating the raw material fishes by adopting a composite biological preservative, and then transferring the fishes to a cold storage for cold storage. The composite biological preservative is prepared from the following raw materials in partsby weight: 5-8 parts of lysozyme, 10-12 parts of nisin, 8-12 parts of fish bone peptide, 8-10 parts of glycerol, 15-20 parts of sodium chloride and 95-105 parts of water. The processing method of fishes provided by the invention has the advantages that good antibacterial performance can be guaranteed and the fishes are easy to clean. Meanwhile, the method has convenient process operation, and short processing time.