Method for inactivating Listeria monocytogenes in food by cooperating ultrahigh pressure with antibacterial agent

Abstract

The invention discloses a method for inactivating Listeria monocytogenes in food by cooperating ultrahigh pressure with an antibacterial agent. The method comprises the following steps of (1) LB1 andLB2 liquid culture medium preparation; (2) Listeria monocytogenes inoculating and activating, wherein the Listeria monocytogenes is inoculated into an LB1 liquid culture medium for culture through a slant culture medium to obtain an LB1 bacterial solution, and the LB1 bacterial solution is taken and added into an LB2 liquid culture medium to obtain an LB2 bacterial solution; (3) ultrahigh pressuretreatment, wherein the antibacterial agent is added into a Listeria monocytogenes solution separately to obtain a bacterium suspension of Listeria monocytogenes, vacuum sterile sealing is conducted on the suspension, and ultrahigh pressure treatment is conducted; (4) counting, wherein the Listeria monocytogenes obtained after ultrahigh pressure treatment is cultured and counted. Accordingly, theinactivation effect is greatly improved, the sublethal damage rate is as low as 15.24%, and the lethal rate is up to 100%; the control over the sublethal state of the Listeria monocytogenes is achieved, and the treatment pressure can be reduced.

Description

technical field [0001] The invention relates to a sterilization method, in particular to a method for inactivating Listeria monocytogenes in food with an ultra-high pressure synergistic antibacterial agent. Background technique [0002] The traditional food industry generally adopts heat sterilization technology, but the heat treatment process will destroy the natural flavor and taste of some foods, and lead to the loss of nutrients. As a non-thermal sterilization technology, ultra-high pressure technology can not only effectively inactivate L.monocytogenes in food, but also better maintain the original natural flavor, taste and color of food, and reduce nutritional material loss. However, Listeria monocytogenes subjected to high pressure stress will appear in a state of sublethal damage, which can complete repair under a suitable environment, showing pathogenicity, and posing a potential hazard to food safety. Contents of the invention [0003] Purpose of the invention:...

AI Technical Summary

Problems solved by technology

However, Listeria monocytogenes subjected to high pressure stress will appear in a state of sub-lethal damage, which can complete repair under a suitable environment, showing pathogenicity, and posing a potential hazard to food safety

Images