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144results about How to "Good inactivation effect" patented technology

Preparation method of soybean milk

The invention relates to a preparation method of soybean milk. The preparation method at least comprises the following stages: (a), flour preparation stage: providing a crushing container, a heating element for heating the crushing container and a crushing device, directly heating the crushing container through the heating element, so as to ensure that the temperature of the crushing container is increased to 70-90 DEG C, adding dried beans into the crushing container, crushing the dried beans into bean flour through the crushing device, and ensuring that lipase and beany flavor of the bean flour are removed in the crushing container at ambient temperature; (b), preparation and boiling stage: adding water to the bean flour, so as to prepare the bean flour into pulp, and heating and boiling the pulp till the pulp is well cooked. By adopting the air temperature inside the crushing device, crushing, lipase removal and beany flavor removal are performed at the same time, protein can keep invariant, beany flavor can be effectively removed, and crushing the beans into bean flour firstly can greatly enhance the crushing fineness, so that the soybean milk concentration can be effectively enhanced. In addition, water vapor can be evaporated during crushing of the beans in the high-temperature environment, the bean flour is not easy to agglomerate, and the crushing effect is good.
Owner:JOYOUNG CO LTD

Process for preparing human serum albumin

The invention discloses a process for preparing human serum albumin. According to the process, a low-temperature ethanol separation method is adopted, and the human serum albumin is prepared from human plasma. The process comprises the steps of dissolving plasma; preparing an ingredient I; preparing an ingredient II and an ingredient III; preparing an ingredient IV; preparing an ingredient V; refining the ingredient V; carrying out ultrafiltration; diluting; carrying out pasteurization; sterilizing and packaging albumen; incubating products; and packaging finished products. The process has the advantages that solid-liquid separation is carried out by adopting a pressure filtration technology, so that the albumin yield which is higher than 29 g / L plasma is increased remarkably, the purity is higher than 98%, and the stability of the products is improved remarkably; Zetaplus deep filter-core filtration is combined with the prolongation of pasteurization time, so that the PKA (Protein Kinase A) level of the products is effectively controlled to be lower than 20IU / ml, and the risks of excessive heat source and virus infection in the products are reduced; and during the process, sodium chloride solutions of two gradient concentrations are used for carrying out ultrafiltration, so that not only can the ethanol residual quantity of the products be controlled to be lower than 0.025%, but also the aluminum residual quantity can be effectively minimized to be lower than 50 micrograms / L.
Owner:华润博雅生物制药集团股份有限公司

Method and device for killing algae and bacteria in pipeline transportation of marine ballast water

The invention provides a method and a device for killing algae and bacteria in the pipeline transportation of marine ballast water. A millipore filter and a tubular high-voltage discharge reaction unit are connected on a transportation pipeline of the marine ballast water in series, the marine ballast water passes through the millipore filter, the diameter of filtration pores is 20 to 50 microns, and larger suspended matters and aquatic organisms entering a pipeline system of the marine ballast water are removed; and the filtered water enters the tubular high-voltage discharge reaction unit, pulse high voltage of 10 to 60 kilovolts is applied between a high-voltage electrode and a ground electrode of the tubular high-voltage discharge reaction unit, high-voltage pulse discharge is generated, the detention time of the ballast water in the tubular high-voltage discharge reaction unit is 0.1 to 2 hours, and the ballast water passing through the tubular high-voltage discharge reaction unit enters a ballast tank. The invention can kill or inactivate foreign pathogens and pests in the ballast water in a pipeline transportation system and lowers or eliminates the marine pollution caused after the ballast water is discharged.
Owner:HARBIN ENG UNIV

Hi-C high-throughput sequencing library construction method applicable to plants

InactiveCN109055491ARealization of Hi-C high-throughput sequencing library constructionSolve the technical problems of poor database construction effectMicrobiological testing/measurementLibrary creationCross-linkEnzyme digestion
The invention relates to a Hi-C high-throughput sequencing library construction method applicable to plants. The method comprises the following steps: (1) performing vacuum formaldehyde cross-linkingon sample ice; (2) cracking the cross-linked material, and releasing cell chromatin; performing endogenous enzyme inactivation on the cell chromatin by SDS (Sodium Dodecyl Sulfate), performing enzymedigestion cutting by MboI endonuclease to obtain a material subjected to enzyme digestion, wherein the concentration of the MboI endonuclease is 5000 units / mL, and the enzyme digestion condition is that the material is incubated overnight at 37 DEG C at 900 revolutions per minute; (3) performing end repair on the material subjected to enzyme digestion; (4) performing DNA intramolecular ligation after end repair; (5) decrosslinking the material subjected to intramolecular ligation, removing the non-ligation terminal biotin to obtain purified DNA, randomly breaking the DNA into fragments favorable for sequencing by ultrasonic waves, so as to obtain the DNA sequencing library. According to the method, by designing the appropriate formaldehyde cross-linking conditions, endogenous enzyme inactivation conditions and appropriate tool enzymes, the technical problems that the cytoplasm is difficultly released from plant cells and the library construction effect of low-quality plant samples is poor are solved, the Hi-C high-throughput sequencing library construction of the plants is realized, and the application range of the Hi-C technology is widened.
Owner:WUHAN FRASERGEN CO LTD

Cereal-based edible tableware and making method thereof

ActiveCN106954663AIncrease gelatinization temperature and gelatinization viscosityPromote aggregationDough treatmentBakery productsMillet flourRice flour
The invention discloses cereal-based edible tableware and a making method thereof. The cereal-based edible tableware comprises cereal powder and auxiliary materials, wherein the cereal powder comprises the following components in parts by weight of 60-80 parts of whole wheat flour, 5-20 parts of sorghum flour, 5-15 parts of rice flour, 0-10 parts of millet flour and 0-10 parts of purple sweet potato flour; and the auxiliary materials comprise the following components in parts by mass: 30-40 parts of water, 3-5 parts of butter and 0-5 parts of seasonings. The making method comprises the following steps of mixing the cereal powder which is subjected to microwave-assisted treatment and then roasting and precooking treatment or dry heat precooking treatment with the auxiliary materials to make dough; putting the made dough in a mold, performing heat-preservation shaping, conveying the shaped dough into an oven, performing roasting, and in the roasting process, performing pressurization for 1-2 times; and performing roasting until shaped dough is 90% cooked, performing film painting with rice flour dextrinization fluid or starch dextrinization fluid of which the concentration is 2-5%, and continuing roasting for 1-2 min so as to obtain finished products. According to the cereal-based edible tableware and the making method thereof disclosed by the invention, used raw materials are natural cereals, are rich in nutrition and can be directly eaten, so that environmental pollution is avoided; the used technology is simple, industrial production is easy to realize, and the cost is low; and the cereal-based edible tableware is compact in texture and is not deformed.
Owner:QINGDAO AGRI UNIV

Device and method for purifying air by using low-temperature plasma activated water

The invention discloses a device and a method for purifying air by using low-temperature plasma activated water. The device comprises a low-temperature plasma generating device and an activated wateratomizing device. The low-temperature plasma generating device comprises a power supply, an insulating container and a plasma generating mechanism arranged in the insulating container. The plasma generation mechanism comprises an electrode array plate, a porous electrode column and a blocking dielectric tube. The electrode array plate comprises a connecting plate and an electrode column array group which is arranged at the bottom of the connecting plate and consists of high-voltage electrode columns. Through holes matched with the high-voltage electrode column are distributed in the porous electrode column, and a barrier dielectric pipe is arranged between the through holes and the high-voltage electrode column. At least two gas inlet holes for loading working gas are symmetrically formedin the upper part of the side wall of the insulating container wall. According to the device and the method, bacteria and fungi can be efficiently inactivated, the inactivation effect on specific species of fungi is remarkable, and most fungi in the air can be efficiently killed within 30 minutes (> 98.5%).
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Trivalent virus antibodies and preparation method thereof

InactiveCN104817640AWide range of protectionProactive preventionEgg immunoglobulinsImmunoglobulins against virusesYolkHand-foot-and-mouth disease
The invention discloses trivalent virus antibodies and a preparation method thereof. The trivalent virus antibodies are trivalent chicken egg-yolk antibodies resisting hand-foot-and-mouth disease viruses, wherein the trivalent hand-foot-and-mouth disease viruses include EV71, CoxA10 and CoxA16. The preparation method comprises the following steps: (1) respectively preparing virus immunizing antigens; (2) injecting the three different virus immunizing antigens into different hens; (3) collecting immunized eggs produced by the immunized hens and extracting and purifying the egg yolks of the immunized eggs so as to respectively obtain three monotype antibody stock solutions; and (4) adding a stabilizer to the three obtained monotype antibody stock solutions according to the antibody titer determination so as to obtain the chicken egg-yolk antibodies. The trivalent virus antibodies provided by the invention are capable of neutralizing tri-type virus antigen and have wider protection coverage; monotype antibodies in a prepared tri-type antibody stock solution are capable of inhibiting and killing antigens independently; the trivalent virus antibodies are stable in bioactivity, high in titer and good in safety, and can be stored for a long time.
Owner:CHENGDU ANTIK BIOTECH CO LTD

Low-temperature plasma generator and method for preparing activated water

The invention discloses a low-temperature plasma generator and a method for preparing the activated water. The device includes a power source, an insulation container and a plasma generating mechanismarranged in the insulation container, wherein the plasma generating mechanism includes an electrode array board, a porous electrode column and a blocking dielectric tube, the electrode array board isconnected with a high-voltage output end of the power source, the porous electrode column is connected with a low-voltage input end of the power source and grounded through a ground wire, the electrode array board includes a connection board and an electrode column array group which consists of high-voltage electrode columns and is arranged at the bottom of the connection board, the porous electrode column is internally distributed with through holes matched with the high-voltage electrode columns, the blocking dielectric tube is arranged between the through holes and the high-voltage electrode columns, and at least two air inlet holes for loading working gas are symmetrically arranged on an upper side wall of the insulation container. The activated water can effectively kill bacteria andfungi live, the inactivation effect on the fungi of a specific species is significant, and most of the fungi (>85.2%) in the liquid can be effectively killed within 1 minutes.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Steam and microwave joint enzyme deactivation method of artemisia integrifolia

InactiveCN104663855ALow costAvoid problems such as yellowingFruits/vegetable preservation by heatingProcess equipmentHeating time
The invention discloses a steam and microwave joint enzyme deactivation method of artemisia integrifolia. The steam and microwave joint enzyme deactivation method comprises the following specific steps: removing impurities from fresh artemisia integrifolia, cleaning, and draining off; firstly, blanching the fresh artemisia integrifolia through a steam generator at 75-100 DEG C for 1-3.0 minutes; and treating the sample which is blanched by steam through a microwave heating device, wherein the power is set to be 300-550W and the heating time is set to be 1-3.0 minutes, and thus finishing the overall enzyme deactivation treatment. Treatment such as color protection, brittleness-keeping and salting is carried out on the deactivated sample, and finally vacuum packing is carried out, so as to obtain the final artemisia integrifolia product. The method disclosed by the invention is reasonable in processing equipment design, appropriate in technological parameter selection, and suitable for enzyme deactivation of leaf vegetables, stem and leaf vegetables and wild vegetables; high energy consumption of a conventional enzyme deactivation method can be reduced; the enzyme inhibition rate can be effectively improved; the plant tissue structure is kept; and meanwhile, the defects that vegetable bodies become soft and yellow, and are relatively large in nutrient loss caused by traditional enzyme deactivation are overcome. According to the method, a basis can be provided for storage and preservation of wild vegetables and food processing and development.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Combined sterilizing and algae-removing technology of sewerage by efficient oxidation process

The combined sterilization alga-removal process to sewage by efficient oxidation process pertains to the chemical oxidation sterilization alga-removal field. The problems of secondary pollution, short inhibition aging, and the like, exist in the present chemical oxidation sterilization alga-removing process. In the process of the invention, ozone with high oxidation activity and chlorine dioxide are combined for sterilization and alga-removing, and the specific steps are that: ozone is passed into the water in suspense (the inlet amount of O3 is 2-10 mg/l water), after the water rests for 48 hours at room temperature and is stirred, then ClO2 gas (the inlet amount of ClO2 gas is 6-10 mg/l water) is passed into the water, after the water rests for 48 hours at room temperature, the qualified water is obtained by filtration. The process of the invention is simple, cost is low, the rate of sterilization alga-removal can reach up to 99%, left poison and second pollution do not exist, disinfection and sterilization alga-removal to the water used in aquaculture, recycling water, water used for townscape, oilfield injection water and hospital sewage terminal can be radically realized.
Owner:BEIJING UNIV OF CHEM TECH

Preparation method of natural source plant antibiotic and application thereof

The invention relates to a preparation method of a natural source plant antibiotic and application thereof. The natural source plant antibiotic is prepared from kernels or shells of fructus crataegi, waxberries, wild jujubes, oranges, walnuts, eriobotrya japonica, fructus jujubae, apricot, peaches, plums, pomelo, pine nuts and filberts which are used as raw materials. The natural source plant antibiotic is wide in antibacterial spectrum and obvious in inactivation effect on virus, fungus, and bacteria, is safe and stable, has the functions of accelerating healing of mucosa wound surfaces, repairing pathological tissue and enhancing natural defense functions, and is capable of effectively preventing and treating harm of pathogenic microorganisms to the human body; meanwhile, the natural source plant antibiotic can be widely applied to multiple fields closely related to the human living environment such as disease prevention, senescence resistance and healthcare, skincare, sanitation supplies, cosmetics, daily chemical products, air sterilization, foods, medicines, feed additives, food additives, deodorants, soil conditioners, control and prevention of pests and improvement of yield of vegetables and crops; and the natural source plant antibiotic has extensive utilization value and development prospect.
Owner:席亚彬

Ultraviolet ray inactivation apparatus and water purifier

The present invention provides an ultraviolet ray inactivation apparatus and a water purifier. The ultraviolet ray inactivation apparatus comprises: a flow falling pipe, wherein the flow falling pipe presents a translucent three-dimensional helical structure, is provided with a flow inlet and a flow outlet, and is used for overflowing of a fluid inactivated by ultraviolet ray; a broad-spectrum ultraviolet ray lamp group, wherein the broad-spectrum ultraviolet ray lamp group comprises one or a plurality of ultraviolet ray lamps, is arranged in the space limited by the three-dimensional helical structure of the flow falling pipe, and is used for radiating ultraviolet rays toward the fluid in the flow falling pipe so as to perform inactivation operation; and a power supply for supplying power for the ultraviolet ray lamp group according to the condition of the fluid in the flow falling pipe. According to the ultraviolet ray inactivation apparatus of the present invention, the broad-spectrum inactivation is achieved while the ultraviolet ray inactivation frequency spectrums can be selected and combined so as to improve the targeting property, the reliability and the safety of the inactivation treatment; and the radiation intensity of the ultraviolet rays can be adjusted according to the fluid condition so as to ensure the inactivation effect and the energy consumption utilization efficiency.
Owner:JIANGSU HAOMAI LIGHTING SCI & TECH CO LTD

Preparation method of vaccine for epidemic encephalitis B

The invention relates to a preparation method of a vaccine for epidemic encephalitis B, which comprises the processes of cell culture, virus propagation and vaccine preparation. After cells overgrow with a single layer, a seed culture of viruses and a culture solution are inoculated, and the use quantity of the seed culture of the viruses is that every 100cm<2> of cells is inoculated with 1ml of seed culture of the viruses. The culture solution of the viruses mainly comprises 90-95% of MEM culture solution, 3-5% of calf serum and 7.5% of sodium bicarbonate solution the pH of which is regulated to 7.2-7.6. The vaccine is prepared by adding human serum albumin with the final concentration of 0.3% after being clarified and filtered. The invention adopts an SA14-14-2 attenuated strain to produce an encephalitis purified vaccine. Compared with a vaccine produced by a P3 strain, the attenuated strain is safer and more effective. The invention has the advantages that: (1) the attenuated strain is used for preparing the purified vaccine, which has good safety; (2) the inactivation effect of inactivating the viruses by using beta-propiolactone is good, and an inactivator is naturally degraded and has no residue; and (3) the column chromatography purification property is mild, and the injury to the viruses is small.
Owner:ZHEJIANG TIANYUAN BIO PHARM CO LTD

Probiotics composition with mammary gland hyperplasia regulating function and application of probiotics composition

The invention discloses a probiotics composition with a mammary gland hyperplasia regulating function. The probiotics composition is prepared from lactobacillus plantarum, lactobacillus acidophilus and bifidobacterium animalis. In the probiotics composition, the content of the lactobacillus plantarum is 5*10<6> to 5*10<9> CFU/g, the content of the lactobacillus acidophilus is 2.5*10<6> to 2.5*10<9> CFU/g, and the content of the bifidobacterium animalis is 2.5*10<6> to 2.5*10<9> CFU/g. The probiotics composition is further cooperated with traditional Chinese medicine extracts to prepare a probiotics traditional Chinese medicine preparation. Furthermore, by combination of components such as the probiotics composition, the traditional Chinese medicine extracts and prebiotics, a product with the mammary gland hyperplasia regulating function can be prepared. The probiotics composition, the probiotics traditional Chinese medicine preparation and the product can promote multiplication of probiotics in a human body and recover balance of female hormones and progestational hormones, further can adjust the intestinal flora of the human body while relieving mammary gland hyperplasia, and is beneficial for the health of the human body.
Owner:泰安大凡神农生物有限公司

Antiallergic gel nose-protecting medical supply and making method thereof

The invention relates to an antiallergic gel nose-protecting medical supply and a making method thereof. The nose-protecting medical supply consists of a nose-shading sheet, antiallergic gel and a natural antipathogenic microorganism biomaterial. The nose-shading sheet consists of a film and a filtering sheet. One surface of the film is sticky. The left side and the right side of the film are provided with a left wing part and a right wing part. One side of the film is provided with a plurality of incision lines. A protruding part is arranged in the center of the film. The filtering sheet is arranged at the position of the protruding part in the center of the film and is tilted upwardly. The film surface of the filtering sheet is smeared with antiallergic gel, while the nose surface of the filtering sheet is smeared with natural antipathogenic microorganism biomaterial. The antiallergic gel nose-protecting medical supply is disposable and decomposable and mist on glasses of a user is prevented. Medical biomaterial in the invention has no toxic or side effect, so the antiallergic gel nose-protecting medical supply is safe for children, pregnant women and drivers and is convenient to use. The antiallergic gel nose-protecting medical supply is made by a simple process, is appropriate in cost, and is environmentally-friendly during production.
Owner:武汉耦合医学科技有限责任公司
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