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144results about How to "Good inactivation effect" patented technology

Preparation method of soybean milk

The invention relates to a preparation method of soybean milk. The preparation method at least comprises the following stages: (a), flour preparation stage: providing a crushing container, a heating element for heating the crushing container and a crushing device, directly heating the crushing container through the heating element, so as to ensure that the temperature of the crushing container is increased to 70-90 DEG C, adding dried beans into the crushing container, crushing the dried beans into bean flour through the crushing device, and ensuring that lipase and beany flavor of the bean flour are removed in the crushing container at ambient temperature; (b), preparation and boiling stage: adding water to the bean flour, so as to prepare the bean flour into pulp, and heating and boiling the pulp till the pulp is well cooked. By adopting the air temperature inside the crushing device, crushing, lipase removal and beany flavor removal are performed at the same time, protein can keep invariant, beany flavor can be effectively removed, and crushing the beans into bean flour firstly can greatly enhance the crushing fineness, so that the soybean milk concentration can be effectively enhanced. In addition, water vapor can be evaporated during crushing of the beans in the high-temperature environment, the bean flour is not easy to agglomerate, and the crushing effect is good.
Owner:JOYOUNG CO LTD

Process for preparing instant powdered naked oats beverage

An instant naked oats powder as solid beverage for the patients of hyperlipomia and diabetes is prepared from naked oats through removing impurities and fine fibres, soaking in hot water, grinding, adding alpha-amylase, auxiliary materials and emulsifier, homogenizing, emulsifying, and spray drying. Its advantages are high dissolving speed and solubility (more than 89.44%), and rich gamma-linolenic acid.
Owner:SHANXI UNIV

Process for preparing human serum albumin

The invention discloses a process for preparing human serum albumin. According to the process, a low-temperature ethanol separation method is adopted, and the human serum albumin is prepared from human plasma. The process comprises the steps of dissolving plasma; preparing an ingredient I; preparing an ingredient II and an ingredient III; preparing an ingredient IV; preparing an ingredient V; refining the ingredient V; carrying out ultrafiltration; diluting; carrying out pasteurization; sterilizing and packaging albumen; incubating products; and packaging finished products. The process has the advantages that solid-liquid separation is carried out by adopting a pressure filtration technology, so that the albumin yield which is higher than 29 g / L plasma is increased remarkably, the purity is higher than 98%, and the stability of the products is improved remarkably; Zetaplus deep filter-core filtration is combined with the prolongation of pasteurization time, so that the PKA (Protein Kinase A) level of the products is effectively controlled to be lower than 20IU / ml, and the risks of excessive heat source and virus infection in the products are reduced; and during the process, sodium chloride solutions of two gradient concentrations are used for carrying out ultrafiltration, so that not only can the ethanol residual quantity of the products be controlled to be lower than 0.025%, but also the aluminum residual quantity can be effectively minimized to be lower than 50 micrograms / L.
Owner:华润博雅生物制药集团股份有限公司

Method and device for killing algae and bacteria in pipeline transportation of marine ballast water

The invention provides a method and a device for killing algae and bacteria in the pipeline transportation of marine ballast water. A millipore filter and a tubular high-voltage discharge reaction unit are connected on a transportation pipeline of the marine ballast water in series, the marine ballast water passes through the millipore filter, the diameter of filtration pores is 20 to 50 microns, and larger suspended matters and aquatic organisms entering a pipeline system of the marine ballast water are removed; and the filtered water enters the tubular high-voltage discharge reaction unit, pulse high voltage of 10 to 60 kilovolts is applied between a high-voltage electrode and a ground electrode of the tubular high-voltage discharge reaction unit, high-voltage pulse discharge is generated, the detention time of the ballast water in the tubular high-voltage discharge reaction unit is 0.1 to 2 hours, and the ballast water passing through the tubular high-voltage discharge reaction unit enters a ballast tank. The invention can kill or inactivate foreign pathogens and pests in the ballast water in a pipeline transportation system and lowers or eliminates the marine pollution caused after the ballast water is discharged.
Owner:HARBIN ENG UNIV

Hi-C high-throughput sequencing library construction method applicable to plants

InactiveCN109055491ARealization of Hi-C high-throughput sequencing library constructionSolve the technical problems of poor database construction effectMicrobiological testing/measurementLibrary creationCross-linkEnzyme digestion
The invention relates to a Hi-C high-throughput sequencing library construction method applicable to plants. The method comprises the following steps: (1) performing vacuum formaldehyde cross-linkingon sample ice; (2) cracking the cross-linked material, and releasing cell chromatin; performing endogenous enzyme inactivation on the cell chromatin by SDS (Sodium Dodecyl Sulfate), performing enzymedigestion cutting by MboI endonuclease to obtain a material subjected to enzyme digestion, wherein the concentration of the MboI endonuclease is 5000 units / mL, and the enzyme digestion condition is that the material is incubated overnight at 37 DEG C at 900 revolutions per minute; (3) performing end repair on the material subjected to enzyme digestion; (4) performing DNA intramolecular ligation after end repair; (5) decrosslinking the material subjected to intramolecular ligation, removing the non-ligation terminal biotin to obtain purified DNA, randomly breaking the DNA into fragments favorable for sequencing by ultrasonic waves, so as to obtain the DNA sequencing library. According to the method, by designing the appropriate formaldehyde cross-linking conditions, endogenous enzyme inactivation conditions and appropriate tool enzymes, the technical problems that the cytoplasm is difficultly released from plant cells and the library construction effect of low-quality plant samples is poor are solved, the Hi-C high-throughput sequencing library construction of the plants is realized, and the application range of the Hi-C technology is widened.
Owner:WUHAN FRASERGEN CO LTD

Cereal-based edible tableware and making method thereof

ActiveCN106954663AIncrease gelatinization temperature and gelatinization viscosityPromote aggregationDough treatmentBakery productsMillet flourRice flour
The invention discloses cereal-based edible tableware and a making method thereof. The cereal-based edible tableware comprises cereal powder and auxiliary materials, wherein the cereal powder comprises the following components in parts by weight of 60-80 parts of whole wheat flour, 5-20 parts of sorghum flour, 5-15 parts of rice flour, 0-10 parts of millet flour and 0-10 parts of purple sweet potato flour; and the auxiliary materials comprise the following components in parts by mass: 30-40 parts of water, 3-5 parts of butter and 0-5 parts of seasonings. The making method comprises the following steps of mixing the cereal powder which is subjected to microwave-assisted treatment and then roasting and precooking treatment or dry heat precooking treatment with the auxiliary materials to make dough; putting the made dough in a mold, performing heat-preservation shaping, conveying the shaped dough into an oven, performing roasting, and in the roasting process, performing pressurization for 1-2 times; and performing roasting until shaped dough is 90% cooked, performing film painting with rice flour dextrinization fluid or starch dextrinization fluid of which the concentration is 2-5%, and continuing roasting for 1-2 min so as to obtain finished products. According to the cereal-based edible tableware and the making method thereof disclosed by the invention, used raw materials are natural cereals, are rich in nutrition and can be directly eaten, so that environmental pollution is avoided; the used technology is simple, industrial production is easy to realize, and the cost is low; and the cereal-based edible tableware is compact in texture and is not deformed.
Owner:QINGDAO AGRI UNIV

Nanoemulsion vaccines

The present invention provides methods and compositions for the stimulation of immune responses. Specifically, the present invention provides nanoemulsion compositions harboring one or more immunogens within the oil phase of the nanoemulsion and methods of using the same for the induction of immune responses (e.g., innate and / or adaptive immune responses (e.g., for generation of host immunity against an environmental pathogen)). Compositions and methods of the invention find use in, among other things, clinical (e.g., therapeutic and preventative medicine (e.g., vaccination)) and research applications.
Owner:NANOBIO CORP +1

Device and method for purifying air by using low-temperature plasma activated water

The invention discloses a device and a method for purifying air by using low-temperature plasma activated water. The device comprises a low-temperature plasma generating device and an activated wateratomizing device. The low-temperature plasma generating device comprises a power supply, an insulating container and a plasma generating mechanism arranged in the insulating container. The plasma generation mechanism comprises an electrode array plate, a porous electrode column and a blocking dielectric tube. The electrode array plate comprises a connecting plate and an electrode column array group which is arranged at the bottom of the connecting plate and consists of high-voltage electrode columns. Through holes matched with the high-voltage electrode column are distributed in the porous electrode column, and a barrier dielectric pipe is arranged between the through holes and the high-voltage electrode column. At least two gas inlet holes for loading working gas are symmetrically formedin the upper part of the side wall of the insulating container wall. According to the device and the method, bacteria and fungi can be efficiently inactivated, the inactivation effect on specific species of fungi is remarkable, and most fungi in the air can be efficiently killed within 30 minutes (> 98.5%).
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Antibacterial and antiviral CoCrCuFeNi high-entropy alloy and in-situ alloying method employing selective laser melting and application thereof

ActiveCN113458418AIn-situ alloyingHas broad-spectrum antibacterial and antiviral capabilitiesAdditive manufacturing apparatusIncreasing energy efficiencySelective laser meltingHigh entropy alloys
The invention discloses an antibacterial and antiviral CoCrCuFeNi high-entropy alloy and an in-situ alloying method employing selective laser melting and application thereof, and belongs to the technical field of high-entropy alloys. The in-situ alloying method employing selective laser melting of the antibacterial and antiviral CoCrCuFeNi high-entropy alloy comprises the following steps: mixing pre-alloyed CoCrFeNi powder and Cu powder to obtain mixed powder; flatly laying the mixed powder layer by layer; and carrying out selective laser melting by adopting laser as an energy source. According to the method, the selective laser melting technology is combined with the antibacterial and antiviral high-entropy alloy, and in-situ alloying is achieved in the selective laser melting process, so that the alloy development efficiency is improved, and the homogeneous single-phase face-centered cubic high-entropy alloy is obtained, and the quasi-equal-atomic-ratio CoCrFeCuNi high-entropy alloy with broad-spectrum antibacterial and antiviral capacity and good mechanical property is prepared.
Owner:NORTHEASTERN UNIV

Equipment and method for inactivating blood component pathogens by riboflavin photochemical method

The invention discloses a method for inactivating blood component pathogens by a riboflavin photochemical method. The method for inactivating blood component pathogens by the riboflavin photochemicalmethod comprises the steps as follows: S1, adding a riboflavin solution with concentration of 50 [mu]mol / L to a blood product to obtain new blood; S2, transferring the blood product under illumination, removing dissolved oxygen in blood components by a nitrogen circulating system, and reducing production of oxygen free radicals; and S3, exposing the obtained new blood under narrow-spectrum ultraviolet light with the wave peak of 309 nm-313 nm, exciting riboflavin to have electron transfer, and damaging DNA and RNA in blood components to achieve the purposes of inactivating pathogens and lymphocytes. Equipment for inactivating blood component pathogens by the riboflavin photochemical method comprises illumination pretreatment equipment, illumination equipment and product storage equipment.The problems of pathogen spreading and leukocyte contamination risks in current blood transfusion are solved.
Owner:BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI

Preparation method of soybean protein peptide

The invention discloses a preparation method of a soybean protein peptide. The preparation method comprises the following steps of firstly, selecting a raw material powdery soybean protein isolate, and then sending the selected powdery soybean protein isolate into water to be dissolved; pretreating a dissolved soybean protein isolate solution, and then adjusting the pH of the soybean protein isolate solution; sending the pH-adjusted soybean protein isolate solution into a container for enzymolysis. By means of the preparation method of the soybean protein peptide, firstly, the solution is subjected to high-pressure and ultrasonic treatment, the structure of the soybean protein is changed, the better and more comprehensive contact between an enzyme and a substrate is promoted, the reactionprocess is accelerated, the time for extracting the soybean protein peptide is shortened, the antioxidant effect of the soybean protein peptide is improved, the temperatures of various positions of the solution during enzyme deactivation are equal, the energy loss is small, the energy is saved, the loss of nutrients can be reduced at last, the storage time of the soybean protein peptide is prolonged, and the better use prospect is brought.
Owner:安徽博悦生物科技有限公司

Method for inactivating viruses in protein solution by adopting ultraviolet irradiation

The invention discloses a method for inactivating viruses in a protein solution by adopting ultraviolet irradiation. The method is characterized by comprising the following steps: S1, preparing the protein solution to be treated in a sealed container; S2, connecting the sealed container with a flow type protein solution ultraviolet inactivating instrument; and S3, switching on an ultraviolet lamp set for irradiating the protein solution, wherein the intensity for irradiating a protein solution flowing tube of ultraviolet rays is 2.0-10.0 mw / cm<2>, the irradiation time is 5s-60min, injecting the protein solution into the flowing tube, and after irradiation, collecting the protein solution with the viruses inactivated. According to the method provided by the invention, the protein solution is sufficiently mixed in a specific device, the ultraviolet rays can carry out inactivation on the microbes including viruses, bacteria and the like, while the inactivating effect is achieved, the active ingredients of protein in the protein solution are also reserved.
Owner:广州白云山拜迪生物医药有限公司

Trivalent virus antibodies and preparation method thereof

InactiveCN104817640AWide range of protectionProactive preventionEgg immunoglobulinsImmunoglobulins against virusesYolkHand-foot-and-mouth disease
The invention discloses trivalent virus antibodies and a preparation method thereof. The trivalent virus antibodies are trivalent chicken egg-yolk antibodies resisting hand-foot-and-mouth disease viruses, wherein the trivalent hand-foot-and-mouth disease viruses include EV71, CoxA10 and CoxA16. The preparation method comprises the following steps: (1) respectively preparing virus immunizing antigens; (2) injecting the three different virus immunizing antigens into different hens; (3) collecting immunized eggs produced by the immunized hens and extracting and purifying the egg yolks of the immunized eggs so as to respectively obtain three monotype antibody stock solutions; and (4) adding a stabilizer to the three obtained monotype antibody stock solutions according to the antibody titer determination so as to obtain the chicken egg-yolk antibodies. The trivalent virus antibodies provided by the invention are capable of neutralizing tri-type virus antigen and have wider protection coverage; monotype antibodies in a prepared tri-type antibody stock solution are capable of inhibiting and killing antigens independently; the trivalent virus antibodies are stable in bioactivity, high in titer and good in safety, and can be stored for a long time.
Owner:CHENGDU ANTIK BIOTECH CO LTD

Low-temperature plasma generator and method for preparing activated water

The invention discloses a low-temperature plasma generator and a method for preparing the activated water. The device includes a power source, an insulation container and a plasma generating mechanismarranged in the insulation container, wherein the plasma generating mechanism includes an electrode array board, a porous electrode column and a blocking dielectric tube, the electrode array board isconnected with a high-voltage output end of the power source, the porous electrode column is connected with a low-voltage input end of the power source and grounded through a ground wire, the electrode array board includes a connection board and an electrode column array group which consists of high-voltage electrode columns and is arranged at the bottom of the connection board, the porous electrode column is internally distributed with through holes matched with the high-voltage electrode columns, the blocking dielectric tube is arranged between the through holes and the high-voltage electrode columns, and at least two air inlet holes for loading working gas are symmetrically arranged on an upper side wall of the insulation container. The activated water can effectively kill bacteria andfungi live, the inactivation effect on the fungi of a specific species is significant, and most of the fungi (>85.2%) in the liquid can be effectively killed within 1 minutes.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Inactivation system by extracting air bundling for veneniferous patient of ÔÇÿnot typical modelÔÇÖ pneumonia to breathein a moment

An instantaneous deactivating system for the exhalated air from SARS patient is composed of a height and direction adjustable sucking hood, air delivering tubes, chemical deactivator, high-temp ultraviolet deactivator, air extractor, and regulator of said sucking hood. Its advantages are simple structure, high effect and high safety.
Owner:ZHEJIANG UNIV +1

Steam and microwave joint enzyme deactivation method of artemisia integrifolia

InactiveCN104663855ALow costAvoid problems such as yellowingFruits/vegetable preservation by heatingProcess equipmentHeating time
The invention discloses a steam and microwave joint enzyme deactivation method of artemisia integrifolia. The steam and microwave joint enzyme deactivation method comprises the following specific steps: removing impurities from fresh artemisia integrifolia, cleaning, and draining off; firstly, blanching the fresh artemisia integrifolia through a steam generator at 75-100 DEG C for 1-3.0 minutes; and treating the sample which is blanched by steam through a microwave heating device, wherein the power is set to be 300-550W and the heating time is set to be 1-3.0 minutes, and thus finishing the overall enzyme deactivation treatment. Treatment such as color protection, brittleness-keeping and salting is carried out on the deactivated sample, and finally vacuum packing is carried out, so as to obtain the final artemisia integrifolia product. The method disclosed by the invention is reasonable in processing equipment design, appropriate in technological parameter selection, and suitable for enzyme deactivation of leaf vegetables, stem and leaf vegetables and wild vegetables; high energy consumption of a conventional enzyme deactivation method can be reduced; the enzyme inhibition rate can be effectively improved; the plant tissue structure is kept; and meanwhile, the defects that vegetable bodies become soft and yellow, and are relatively large in nutrient loss caused by traditional enzyme deactivation are overcome. According to the method, a basis can be provided for storage and preservation of wild vegetables and food processing and development.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Combined sterilizing and algae-removing technology of sewerage by efficient oxidation process

The combined sterilization alga-removal process to sewage by efficient oxidation process pertains to the chemical oxidation sterilization alga-removal field. The problems of secondary pollution, short inhibition aging, and the like, exist in the present chemical oxidation sterilization alga-removing process. In the process of the invention, ozone with high oxidation activity and chlorine dioxide are combined for sterilization and alga-removing, and the specific steps are that: ozone is passed into the water in suspense (the inlet amount of O3 is 2-10 mg / l water), after the water rests for 48 hours at room temperature and is stirred, then ClO2 gas (the inlet amount of ClO2 gas is 6-10 mg / l water) is passed into the water, after the water rests for 48 hours at room temperature, the qualified water is obtained by filtration. The process of the invention is simple, cost is low, the rate of sterilization alga-removal can reach up to 99%, left poison and second pollution do not exist, disinfection and sterilization alga-removal to the water used in aquaculture, recycling water, water used for townscape, oilfield injection water and hospital sewage terminal can be radically realized.
Owner:BEIJING UNIV OF CHEM TECH

Preparation method of natural source plant antibiotic and application thereof

The invention relates to a preparation method of a natural source plant antibiotic and application thereof. The natural source plant antibiotic is prepared from kernels or shells of fructus crataegi, waxberries, wild jujubes, oranges, walnuts, eriobotrya japonica, fructus jujubae, apricot, peaches, plums, pomelo, pine nuts and filberts which are used as raw materials. The natural source plant antibiotic is wide in antibacterial spectrum and obvious in inactivation effect on virus, fungus, and bacteria, is safe and stable, has the functions of accelerating healing of mucosa wound surfaces, repairing pathological tissue and enhancing natural defense functions, and is capable of effectively preventing and treating harm of pathogenic microorganisms to the human body; meanwhile, the natural source plant antibiotic can be widely applied to multiple fields closely related to the human living environment such as disease prevention, senescence resistance and healthcare, skincare, sanitation supplies, cosmetics, daily chemical products, air sterilization, foods, medicines, feed additives, food additives, deodorants, soil conditioners, control and prevention of pests and improvement of yield of vegetables and crops; and the natural source plant antibiotic has extensive utilization value and development prospect.
Owner:席亚彬

Ultraviolet ray inactivation apparatus and water purifier

The present invention provides an ultraviolet ray inactivation apparatus and a water purifier. The ultraviolet ray inactivation apparatus comprises: a flow falling pipe, wherein the flow falling pipe presents a translucent three-dimensional helical structure, is provided with a flow inlet and a flow outlet, and is used for overflowing of a fluid inactivated by ultraviolet ray; a broad-spectrum ultraviolet ray lamp group, wherein the broad-spectrum ultraviolet ray lamp group comprises one or a plurality of ultraviolet ray lamps, is arranged in the space limited by the three-dimensional helical structure of the flow falling pipe, and is used for radiating ultraviolet rays toward the fluid in the flow falling pipe so as to perform inactivation operation; and a power supply for supplying power for the ultraviolet ray lamp group according to the condition of the fluid in the flow falling pipe. According to the ultraviolet ray inactivation apparatus of the present invention, the broad-spectrum inactivation is achieved while the ultraviolet ray inactivation frequency spectrums can be selected and combined so as to improve the targeting property, the reliability and the safety of the inactivation treatment; and the radiation intensity of the ultraviolet rays can be adjusted according to the fluid condition so as to ensure the inactivation effect and the energy consumption utilization efficiency.
Owner:JIANGSU HAOMAI LIGHTING SCI & TECH CO LTD

Treatment of organic matter

A method for treatment of organic matter, and inorganic matter that has been biologically contaminated, such as human cadavers, animal carcasses and clinical waste, to prepare the matter for burial or other disposal is disclosed. The method involves freeze-drying the size-reduced organic matter whilst subjecting the partially-dehydrated remains to a series of vacuum-pressure cycles. Process conditions are chosen so as to favour microbial inactivation.
Owner:MORRIS WATSON MICHAEL +2

Soya-bean milk production method with efficient pulverization and noise reduction

The invention relates to a soya-bean milk production method with efficient pulverization and noise reduction. The method at least comprises the following steps: (a) a step of pre-heating and soaking, in which, water and materials are added in a heating cup of a heating apparatus, a pre-set temperature no less than 70 DEG C is achieved by heating at a heating rate in a range of 3 DEG C per minute to 10 DEG C per minute, and the next step is carried out; (b) a step of pulverization, in which, water and materials in the cup are pulverized into a thick liquid through rotation of a pulverization blade driven by an electric motor, and the next step is carried out; and (c) a step of cooking, in which, the thick liquid in the cup of the heating apparatus is heated until the thick liquid is fully cooked. Through comparison with the prior art, according to the technical scheme, the method enables the water absorption and expansion rate between epidermis and cotyledon of soya-beans to be preferably uniform, so that the soya-beans can fully absorb water and swell. The method has improved pulverization efficiency and has the function of noise reduction.
Owner:JOYOUNG CO LTD

Composite disinfectant for silkworm

The invention relates to a composite disinfectant used on silkworm, formed by symclosene and tertiary sodium phosphate, wherein, the mass ration between the symclosene and tertiary sodium phosphate is 40-70:60-30. When in use, the disinfectant is diluted by water into 200-1600 times. The inventive disinfectant can disinfect the causal agent of silkworm, less excite smell, low corrosion, high stability, and long durable period. The inventive solution is transparent with low degrade speed of effective chlorine.
Owner:中国农业科学院蚕业研究所

Production of Immune-Response-Stimulating Aerosols By Non-Thermal Plasma Treatment Of Airborne Pathogens

Infectious diseases can be transmitted to humans, or between humans and animals, by airborne viruses and bacteria, known as infectious aerosols. Current protective measures that individuals can take to avoid inhaling such aerosols are either marginally effective (personal face masks) or impractical (self-contained breathing apparatuses). Building ventilation systems employing high-efficiency filters to prevent distribution of such aerosols suffer from high energy costs and high filter replacement costs. The development of conventional, intramuscularly administered vaccines takes months or years to produce enough doses to protect a population from a rapidly spreading infectious disease. Airborne viruses and bacteria have been shown to be completely inactivated when exposed to non-thermal plasmas. Results indicate the potential for sub-lethal exposures of airborne pathogens could render them unable to spark an infection in a host, but still retain the necessary surface proteins to cause an immune response in the host.
Owner:RGT UNIV OF MICHIGAN

Quick-frozen sweet corn grains and preparing method thereof

InactiveCN105686029AShort blanching timeIncrease the gapFood shapingAdditive ingredientClean water
The invention discloses quick-frozen sweet corn grains and a preparing method thereof. The preparing method is characterized by including the following step of blanching, wherein sweet corn grains are put into boiling clean water to be blanched, the ratio of water to the sweet corn grains is 4:1-3:1, the temperature is 96-98 DEG C, and the blanching time is 75-85 s. By adopting the preparing method of the quick-frozen sweet corn grains, the blanching time is only as short as 75-85 s, and the problems that in the prior art, the blanching time of the sweet corn grains is long, nutritional ingredients of the prepared sweet corn grains are lost seriously, and quality indexes such as color and mouthfeel of the finished product are greatly reduced are solved effectively.
Owner:宁夏天瑞产业集团现代农业有限公司

Preparation method of vaccine for epidemic encephalitis B

The invention relates to a preparation method of a vaccine for epidemic encephalitis B, which comprises the processes of cell culture, virus propagation and vaccine preparation. After cells overgrow with a single layer, a seed culture of viruses and a culture solution are inoculated, and the use quantity of the seed culture of the viruses is that every 100cm<2> of cells is inoculated with 1ml of seed culture of the viruses. The culture solution of the viruses mainly comprises 90-95% of MEM culture solution, 3-5% of calf serum and 7.5% of sodium bicarbonate solution the pH of which is regulated to 7.2-7.6. The vaccine is prepared by adding human serum albumin with the final concentration of 0.3% after being clarified and filtered. The invention adopts an SA14-14-2 attenuated strain to produce an encephalitis purified vaccine. Compared with a vaccine produced by a P3 strain, the attenuated strain is safer and more effective. The invention has the advantages that: (1) the attenuated strain is used for preparing the purified vaccine, which has good safety; (2) the inactivation effect of inactivating the viruses by using beta-propiolactone is good, and an inactivator is naturally degraded and has no residue; and (3) the column chromatography purification property is mild, and the injury to the viruses is small.
Owner:ZHEJIANG TIANYUAN BIO PHARM CO LTD

Probiotics composition with mammary gland hyperplasia regulating function and application of probiotics composition

The invention discloses a probiotics composition with a mammary gland hyperplasia regulating function. The probiotics composition is prepared from lactobacillus plantarum, lactobacillus acidophilus and bifidobacterium animalis. In the probiotics composition, the content of the lactobacillus plantarum is 5*10<6> to 5*10<9> CFU / g, the content of the lactobacillus acidophilus is 2.5*10<6> to 2.5*10<9> CFU / g, and the content of the bifidobacterium animalis is 2.5*10<6> to 2.5*10<9> CFU / g. The probiotics composition is further cooperated with traditional Chinese medicine extracts to prepare a probiotics traditional Chinese medicine preparation. Furthermore, by combination of components such as the probiotics composition, the traditional Chinese medicine extracts and prebiotics, a product with the mammary gland hyperplasia regulating function can be prepared. The probiotics composition, the probiotics traditional Chinese medicine preparation and the product can promote multiplication of probiotics in a human body and recover balance of female hormones and progestational hormones, further can adjust the intestinal flora of the human body while relieving mammary gland hyperplasia, and is beneficial for the health of the human body.
Owner:泰安大凡神农生物有限公司

Antiallergic gel nose-protecting medical supply and making method thereof

The invention relates to an antiallergic gel nose-protecting medical supply and a making method thereof. The nose-protecting medical supply consists of a nose-shading sheet, antiallergic gel and a natural antipathogenic microorganism biomaterial. The nose-shading sheet consists of a film and a filtering sheet. One surface of the film is sticky. The left side and the right side of the film are provided with a left wing part and a right wing part. One side of the film is provided with a plurality of incision lines. A protruding part is arranged in the center of the film. The filtering sheet is arranged at the position of the protruding part in the center of the film and is tilted upwardly. The film surface of the filtering sheet is smeared with antiallergic gel, while the nose surface of the filtering sheet is smeared with natural antipathogenic microorganism biomaterial. The antiallergic gel nose-protecting medical supply is disposable and decomposable and mist on glasses of a user is prevented. Medical biomaterial in the invention has no toxic or side effect, so the antiallergic gel nose-protecting medical supply is safe for children, pregnant women and drivers and is convenient to use. The antiallergic gel nose-protecting medical supply is made by a simple process, is appropriate in cost, and is environmentally-friendly during production.
Owner:武汉耦合医学科技有限责任公司
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