The invention provides a preparation method and application of conductive ink based on metal nanowire and graphene oxide. The graphene oxide is simultaneously used as a dispersing agent, a thickening agent and a stabilizing agent. The metal nanowire, the graphene oxide, the deionized water, the alcohol solvent, the dispersing agent, the surface active agent, the flatting agent and the de-foaming agent are uniformly mixed at a certain mass ratio, so as to acquire the conductive ink product. The acquired ink can be directly written or printed on various substrate materials so as to construct a conductive circuit with high conductivity, stability, flexibility and high adhesion; the constructed conductive circuit is dried for 3-10 minutes under room temperature, so that ultrahigh conductivity is achieved; furthermore, the graphene oxide is reduced and the metal nanowire is sintered according to the methods, such as, selecting sintering temperature at 50-200 DEG C or performing chemical reduction under room temperature or utilizing an xenon flashlight to sinter, so that the conductivity of the conductive circuit is further increased.