Trivalent virus antibodies and preparation method thereof

Abstract

The invention discloses trivalent virus antibodies and a preparation method thereof. The trivalent virus antibodies are trivalent chicken egg-yolk antibodies resisting hand-foot-and-mouth disease viruses, wherein the trivalent hand-foot-and-mouth disease viruses include EV71, CoxA10 and CoxA16. The preparation method comprises the following steps: (1) respectively preparing virus immunizing antigens; (2) injecting the three different virus immunizing antigens into different hens; (3) collecting immunized eggs produced by the immunized hens and extracting and purifying the egg yolks of the immunized eggs so as to respectively obtain three monotype antibody stock solutions; and (4) adding a stabilizer to the three obtained monotype antibody stock solutions according to the antibody titer determination so as to obtain the chicken egg-yolk antibodies. The trivalent virus antibodies provided by the invention are capable of neutralizing tri-type virus antigen and have wider protection coverage; monotype antibodies in a prepared tri-type antibody stock solution are capable of inhibiting and killing antigens independently; the trivalent virus antibodies are stable in bioactivity, high in titer and good in safety, and can be stored for a long time.

Description

technical field [0001] The invention relates to the technical field of biological antibody immunization, in particular to a trivalent virus antibody and a preparation method thereof. Background technique [0002] Hand, foot and mouth disease is an infectious disease in children. There are a variety of enteroviruses that can cause hand, foot and mouth disease. The most common types are CoxA16, EV71 and CoxA10. Its infection routes include digestive tract, respiratory tract and contact transmission. It mostly occurs in children under 5 years old, and the incubation period is mostly 2 to 10 days. It can cause herpes on the hands, feet, mouth and other parts. A small number of children can cause complications such as myocarditis, pulmonary edema, and aseptic meningoencephalitis. The main mode of transmission is through air droplets. If the disease develops rapidly in severely ill children, it will lead to death. For hand, foot and mouth disease, there is no preventive vaccine ...

AI Technical Summary

Problems solved by technology

[0005] There are bivalent HFMD virus egg yolk antibodies (CoxA16, EV71), but they cannot produce blocking and preventive protection against the CoxA10 HFMD virus that is prevalent in some areas, and the antigen used to prepare the antibody is a compound immune antigen (Immune the hen after mixing the bivalent antigen), the titer of the monotype antibody produced by it varies with the difference of the hen body, and the protective effect of the monotype antibody cannot be guaranteed

[0006] There are existing methods for preparing egg yolk antibodies. Although the immunized chickens are healthy laying hens that have been selected, inspected and vaccinated against certain diseases, there are still some chicken-derived bacteria or / and viruses mixed in the eggs to enter Possibility in yolk antibody, such as Escherichia coli, Salmonella, chicken egg drop syndrome virus, chicken avian leukemia virus, etc., thus affecting the safety of the antibody

At present, some studies use toxic formaldehyde, divinylamine (BEI) and β-propiolactone (BPL) as inactivators, which have inactivation effects on exogenous viruses in egg yolk antibodies, but have a negative effect on the activity of egg yolk antibodies. affect the effect of the effect

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