38 results about "Viral antigen" patented technology

The invention discloses an anti-pestivirus broad-spectrum neutralizing monoclonal antibody and application thereof, and belongs to the field of immune globulin. The anti-pestivirus monoclonal antibody or the antigen binding fragment thereof comprises a heavy chain variable region (SEQ ID No.1) and a light chain variable region (SEQ ID No.3). The monoclonal antibody provided by the invention can identify hog cholera virus and all known plague virus members (BVDV1, BVDV2, BDV, HoBiPeV, AydinPeV and OVPV) capable of infecting cattle and sheep at present, has good neutralizing ability, and can be prepared into a diagnostic product for plague virus antigen-antibody detection. The monoclonal antibody and a diagnostic product developed based on the monoclonal antibody can be used for serological diagnosis and immune effect evaluation of pestivirus, pestivirus pathogen detection of experimental serum products and the like, and have wide application prospects in the fields of clinical diagnosis and the like.

The present application provides a vaccine comprising: 1) a peptide oligomer consisting of 2-8 copies of a bacteriophage T4 fibrin C-terminal polypeptide and one or more antigen proteins; or 2) a coding nucleic acid of item 1) wherein 2-8 copies of the bacteriophage T4 fibrin C-terminal polypeptides in the peptide oligomer are directly linked in series through chemical bonds or each of the bacteriophage T4 fibrin C-terminal polypeptides is linked to each other through a peptide linker, and the peptide oligomer and the antigen protein are directly connected through a chemical bond or connected through a peptide linker. In the bi-T4-virus antigen vaccine platform provided by the invention, the antigen can be subjected to multi-component change, that is, the antigen can form a trimer, so that the use flexibility of the vaccine platform is enhanced, the application range of the vaccine platform is widened, and the bi-T4 structure can enhance the formation of trimer protein and enhance the immune aging.

The invention relates to the technical field of biological products, in particular to an indirect ELISA (Enzyme-Linked Immunosorbent Assay) detection kit and a detection method for a goose astrovirus antibody. The kit comprises an elisa plate, an elisa secondary antibody, goose astrovirus positive control serum, goose astrovirus negative control serum, a diluent, a washing solution, a developing solution and a stop solution, wherein a coating antigen of the ELISA plate is a goose astrovirus VP27 recombinant protein, the goose astrovirus is a goose astrovirus GAstV / SD / 36, and the goose astrovirus VP27 recombinant protein corresponds to an ORF2 region. The goose astrovirus GAstV / SD / 36 is used as a template, the virus antigen recognition site VP27 protein is selected as an antigen for preparing the indirect ELISA kit, the indirect ELISA detection kit of the goose astrovirus antibody is prepared, and the accuracy of the detection result of the kit can be improved.

The invention relates to the technical field of animal vaccines, in particular to a recombinant porcine epidemic diarrhea virus S1 protein and transferrin fusion protein as well as a preparation method and application thereof. According to the present invention, the SpyTag sequence is inserted into the S1 gene sequence, the eukaryotic cell HEK293 is adopted to express the protein, such that the obtained recombinant S1 protein has sufficient glycosylation modification, and when the recombinant S1 protein is mixed with the recombinant transferrin containing the SpyCatcher sequence, the K31 on the SpyTag and the N117 on the SpyCatcher are subjected to spontaneous condensation to form the isomeric peptide so as to display the antigen on the transferrin 24 polymer surface; the technical system not only maintains the natural conformation of the virus antigen protein, but also has the advantage of stimulating the innate and adaptive immune response functions of the host, and has a better protection effect on the host.

This invention relates to antisense molecules and methods for regulating the splicing of polyomavirus T antigen precursor mRNA. In one aspect, the invention relates to antisense oligonucleotides of 12 to 30 nucleotides, preferably 17, 18, 19, or 20 to 30 nucleotides, comprising a sequence that is a continuously extended, inversely complementary sequence of at least 12 nucleotides of polyomavirus T antigen precursor mRNA, and that the antisense oligonucleotide can regulate the splicing of the large T-antigen precursor mRNA in cells.

The present invention relates to: a vector capable of simultaneously producing an antigen of porcine circovirus 2d, recently prevalent in Korea, an antigen of porcine circovirus 2a, which has caused diseases from the past to the present, a porcine circovirus 2 cellular immunity recombinant protein, and a p65 recombinant protein of Mycoplasma hyopneuminiae, which causes porcine mycoplasma pneumonia; a vaccine against PCV2 and Mycoplasma, the vaccine comprising four types of antigens, Mycoplasma hyopneuminiae dead cells, and Mycoplasma hyorhinis dead cells produced by the vector; a method for producing same; and a use thereof.

Nucleic acid molecules and compositions comprising one or more nucleic acid sequences that encode a consensus Ebolavirus glycoproteinimmunogens are disclosed. The coding sequences optionally include operable linked coding sequence that encode a signal peptide. Immunomodulatory methods and methods of inducing an immune response against Ebolavirus are disclosed. Method of preventing Ebolavirus and methods of treating individuals infected with Ebolavirus are disclosed. Consensus Ebolavirus proteins are disclosed.

The invention relates to antisense molecules and methods for modulating splicing of polyomavirus T antigen pre-mRNA. In one aspect the invention relates to an antisense oligonucleotide 12 to 30, preferably 17, 18, 19 or 20 to 30 nucleobases in length which comprises a sequence that is the reverse complement of a contiguous stretch of at least 12 nucleobases of a polyomavirus T-antigen pre-mRNA and which antisense oligonucleotide can modulate splicing of said T-antigen pre-mRNA in a cell.

A method for treating a patient comprising: (a) providing a composition comprising a population of T cells expressing both a chimeric antigen receptor (CAR) and a T cell receptor specific for a cytomegalovirus (CMV) antigen; (b) administering the composition to the patient; and (c) administering to the patient a viral vector encoding: (i) CMV pp65 and (ii) a fusion protein comprising exon 4 of CMV protein IE1 (e4) and exon 5 of CMV protein IE2 (e5) either prior to or subsequent to administering the composition comprising a population of T cells to the patient is described.

This disclosure provides a method of protecting a canine against canine distemper virus, the method comprising administering a recombinant oral vaccine comprising a modified live canine adenovirus type 2 (CAV-2) vector carrying a Canine Distemper Virus (CDV) antigen to said canine, wherein the vaccine is administered orally.

The invention refers to a dumbbell-shaped DNA vector comprising a transcriptional promoter, a sequence for expressing an RNA, and a transcriptional terminator The sequence for expressing an RNA may be selected from the group consisting of an ornithine transcarbamylase, a phenylalanine hydroxylase (PAH), and a viral antigen.

This invention discloses a blow-type sampling viral antigen detection kit and its usage method, including a sampler, a blow nozzle, a detection working column, and a equilibration liquid dropper. Sampling is performed by blowing air, a simple sampling method that reduces the probability of sampling errors in untrained individuals. Equilibration liquid is dripped into the funnel-shaped opening of the sampler's receiving tank through the equilibration liquid dropper, continuously eluting the biological sample. The operation is simple and effective. The equilibration liquid, along with the eluted biological sample, enters a colloidal gold filter. The colloidal gold is re-dissolved, captures and labels the viral antigen in the solution. The labeled antigen then enters an antibody filter impregnated with specific antibodies, where it is captured by those antibodies, forming a conjugate of antibody, antigen, and colloidal gold microparticles. A large number of these conjugate microparticles aggregate on the filter membrane above the absorbent cotton column, resulting in color development. The sample elution step is simple, the sampling method is reliable, and the detection and colorimetric observation are easy to perform, making it convenient for the general public to use.

Provided herein are compositions comprising T cells expressing a recombinant virus-specific T cell receptor (rTCR-V) specific for a viral antigen restricted by a predetermined HLA type, rTCR-Vs, methods for preparing them, their use for treatment, and libraries containing such rTCR-V or T cells comprising them.

The present invention relates to antisense molecules and methods for modulating polyoma virus T antigen precursor mRNA splicing. In one aspect, the invention relates to an antisense oligonucleotide having a length of 12 to 30 nucleobases, preferably 17, 18, 19 or 20 to 30 nucleobases, comprising a sequence which is a continuously extending reverse complementary sequence of at least 12 nucleobases of a polyoma virus T antigen precursor mRNA, and which antisense oligonucleotide can modulate splicing of said large T-antigen precursor mRNA in a cell.

The application discloses an epitope tandem polypeptide of porcine reproductive and respiratory syndrome and related biological materials and application thereof, and belongs to the field of viral antigen vaccines or biomedical technologies. The application provides an epitope tandem polypeptide which is composed of neutralizing antigen epitopes, Th epitopes and CTL epitopes of multiple structural proteins and non-structural proteins of PRRSV in series, and includes PNB, PTH and PCTL. The epitope tandem polypeptide provided by the application can be directly used for preparing a subunit vaccine of PRRSV, and has the advantages of simple preparation method, strong immunogenicity and good safety. The epitope tandem polypeptide can also be combined with an inactivated vaccine of PRRSV and applied to enhance cellular immunity induced by the inactivated vaccine, so that the epitope tandem polypeptide can more effectively prevent and protect pigs from PRRSV infection. The epitope tandem polypeptide composition contains common epitope sequences of seven representative strains (LV, VR-2332, CH-1a, JXA1, NADC30, NADC34 and RFLP 1-4-4) of PRRSV, and is expected to have good cross-protection for infection of different strains.

The virus detection device, preparation method, and virus detection method provided by the embodiments of the present invention can directly modify the gate metal in the top metal material layer of the metal-oxide semiconductor field-effect transistor chip layer, using nucleic acid probes, viral antibodies, or viral antigens for virus detection. Compared with existing electrochemical biosensors, this device does not require the introduction of electrochemically active identifiers as markers and is compatible with current mainstream MOSFET processing technology, laying a solid foundation for large-scale commercial production.

The invention discloses a method for recovering adipogenic gene expression and adipogenic capacity of bovine immortalized adipocytes based on siRNA (small interfering Ribonucleic Acid). In order to solve the problems that bovine immortalized adipocytes established by overexpression of SV40 T-antigen genes are inhibited in adipogenesis related gene expression and abnormal in adipogenesis ability, the invention inhibits the expression of SV40 virus T-antigen in bovine adipocytes by designing, screening and identifying specific efficient interfering siRNA (small interfering ribonucleic acid) aiming at the SV40 virus T-antigen. Therefore, the inhibition effect of SV40 on adipogenic gene expression is relieved, the expression level and the adipogenic capacity of the adipogenic gene in the bovine immortalized adipocytes are recovered, and the method plays a key role in breaking through the bottleneck problem of developing species-specific adipodifferentiation mechanism research by utilizing the bovine immortalized adipocytes and getting rid of the current situation of developing research by depending on a mode animal cell line for a long time.

The strip systems, methods, devices and associated kits disclosed herein are used to determine the presence and / or level a target analyte(s) in sample (e.g., a biological sample such as saliva or nasal swab) wherein the target analyte(s) may be a microorganism (e.g., a whole virus) or molecule (e.g., a viral antigen) associated with a healthy state, disease or injury or otherwise altered physiological condition. In certain embodiments, the systems, methods, devices and kits provide one or more improved properties relative to the lateral flow protein and other assays known in the art for the detection, including but not limited to, assay time, ease of use, risk of infection, accuracy, specificity, selectivity, limit of detection of the assay, quantitative detection and the effect of common interferents to the sensor output, cost, simplicity or a combination thereof. In certain embodiments, the systems, assays, methods and kids are multiplexed, i.e., permit detection or monitoring of more than one target analyte (e.g., two different viruses or a virus and a bacterium).

The present invention belongs to the field of biomedical technology, and discloses an aquatic animal antigen system and its application. In the present invention, bacterial cellulose is carboxylated to obtain carboxylated bacterial nanocellulose, and then it is grafted with an aldehyde polymer to obtain surface-modified nanocellulose (i.e., poly(N-isopropylacrylamide) grafted carboxylated bacterial nanocellulose). Using the surface-modified nanocellulose as a carrier, it is chemically linked with a viral antigen protein (i.e., recombinant G protein) to prepare an antigen system carrying the viral antigen protein (i.e., BNC-G). The antigen system can deliver the viral antigen protein into the body of aquatic animals through immersion immunization, achieving the effect of preventing and controlling viral diseases of aquatic animals, and is applicable to the production of SPF fry of aquatic animals and the preparation of immunoprotective agents for aquatic animals.

A gold nanoprobe for detecting a SARS-CoV-2 antigen, a preparation method therefor, and a use thereof. The preparation method comprises the following steps: S1: mixing an HAuCl4 solution boiling at standard atmospheric pressure with a sodium citrate solution, and fully reacting until the mixture becomes red to obtain gold nanospheres; S2: mixing the gold nanospheres, the HAuCl4 solution, and the sodium citrate of step S1 with hydroquinone, and fully reacting to obtain gold nanoflowers; and S3: mixing a SARS-COV-2 monoclonal antibody with the gold nanoflowers of step S2, and fully reacting to obtain gold nanoprobes. The gold nanoflowers thus prepared have a particle size of 55-75 nm, a high specific surface area, and a high extinction coefficient, thus forming a higher contrast. The gold nanoflowers and the monoclonal antibody for specifically recognizing the SARS-CoV-2 N antigen form the gold nanoprobes by means of electrostatic adsorption, thereby improving the sensitivity of test paper.

The application discloses a shark-derived single-domain antibody binding SARS-CoV-2 RBD, the amino acid sequence of the single-domain antibody is shown as SEQ ID NO: 2 or SEQ ID NO: 4; nucleotides encoding the above-mentioned shark-derived single-domain antibody, characterized in that the nucleotide sequence is shown as SEQ ID NO: 1 or SEQ ID NO: 3; the application also provides a preparation method of the shark-derived single-domain antibody binding SARS-CoV-2 RBD, and the shark-derived single-domain antibody is obtained by sequentially performing immunization, constructing a phage antibody library, screening positive clones from the phage antibody library, finally adding an inducer for expression after large-scale culture, and collecting and purifying antibody proteins; the shark-derived single-domain antibody prepared by the application has good application prospects in the development of a new coronavirus disinfecting spray and a new coronavirus antigen detection kit.

The present invention provides an ELISA kit for detecting total antibodies against novel coronavirus, which comprises a coated plate coated with novel coronavirus antigen, an enzyme conjugate containing HRP-labeled mouse anti-human kappa light chain antibody, a system reaction solution, a negative and positive control solution, and a TMB substrate solution. It can quickly and accurately determine the presence of antibodies against novel coronavirus in a sample. The product uses the indirect method to detect total antibodies, which can reduce the production material cost compared with the sandwich method, and the labeling process is more concise.

Early Nasopharyngeal Carcinoma (NPC) detection methods, devices, and kits are provided utilizing novel Epstein-Barr Virus (EBV) EBNA1 antigens to detect serum anti-EBNA1 IgA antibodies with superior correlation to NPC development within four years.

A method for treating a patient comprising: (a) providing a composition comprising a population of T cells expressing both a chimeric antigen receptor (CAR) and a T cell receptor specific for a cytomegalovirus (CMV) antigen; (b) administering the composition to the patient; and (c) administering to the patient a viral vector encoding: (i) CMV pp65 and (ii) a fusion protein comprising exon 4 of CMV protein IE1 (e4) and exon 5 of CMV protein IE2 (e5) either prior to or subsequent to administering the composition comprising a population of T cells to the patient is described.

A method for measuring a viral antigen using a capture antibody and a detection antibody. The method includes forming a sandwich immune complex that contains the capture antibody, the viral antigen and the detection antibody. The capture antibody includes specific sequences of CDRs and the detection antibody includes specific sequences of CDRs.

The application provides a recombinant hepatitis virus antigen, genes and kits, and belongs to the technical field of animal disease detection. The amino acid sequence of the recombinant hepatitis virus antigen is shown as SEQ ID No. 1. The recombinant hepatitis virus antigen provided by the application has the characteristics of high purity and high specificity, can effectively reduce the cross-reaction between reagents, and has better specificity. The high-specificity protein effectively reduces the background value of negative samples, avoids the use of double-antigen detection, is simpler to detect, uses less samples, and is more conducive to the judgment of results. Compared with the same ELISA detection kit on the market, the overall performance of the kit has the advantages of short detection time, higher sensitivity, simpler result judgment and the like.

The present disclosure provides a method of preventing foot lesions caused by FMD virus infection in ruminants by using a vaccine presented as an emulsion containing one or more adjuvants and a viral antigen.

The present invention relates anti-microbial antibody signatures related to lung tumors, including benign tumors and adenocarcinomas. Disclosed herein are antibody panels comprising antigens from bacteria and / or viruses for use in early and accurate diagnosis of lung cancer and for reducing false positive lung carcinoma diagnoses from CT screening.

The utility model discloses a Raman spectrometer for antigen protein detection, which comprises a horizontally placed base, a sample table which is arranged on the base and moves along a straight line in the horizontal direction, and a laser which is arranged above the sample table and emits laser towards the sample table, the clamping groove is matched with a test strip dripped with a sample solution to be detected in size so as to accommodate the test strip; and the power device is used for controlling the sample table to move along a straight line and driving the sample table to move relative to the laser so as to ensure that the laser is focused on the specific position of the test strip on the sample table and realize Raman spectrum analysis on the specific position of the test strip. The Raman spectrometer is simple in structure, and errors caused by manual operation can be reduced, so that the use portability and the detection accuracy are both considered, and rapid and accurate detection of the virus antigen protein is realized.

Described herein are compositions comprising particles, the particles comprising a multilayer film, the multilayer film comprising two or more layers of polyelectrolytes, adjacent layers comprising oppositely charged polyelectrolytes, one of the polyelectrolytes comprising a designed polypeptide represented by the structure: (Pam3Cys or Pam2Cys)-(surface adsorption region 1)-(RSV-G peptide epitope)-L1-(RSV-M2 peptide epitope)-L2-(surface adsorption region 2).