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Preparation of antigen-loaded polymer lipid nanosphere and application as vaccine adjuvant

A technology for polymers and polymer degradation, which is applied in the field of medicine to achieve the effects of increased intake, uniform size, and enhanced activation level

Active Publication Date: 2016-12-07
王连艳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, a variety of microparticle sustained and controlled release systems based on synthetic polymers and natural component materials have been developed at home and abroad, including: oil-in-water emulsions, liposomes, microspheres and nanoparticles, etc., especially in immunology research , the challenges still faced in the study of using microparticle sustained and controlled release system for immune adjuvants

Method used

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  • Preparation of antigen-loaded polymer lipid nanosphere and application as vaccine adjuvant
  • Preparation of antigen-loaded polymer lipid nanosphere and application as vaccine adjuvant
  • Preparation of antigen-loaded polymer lipid nanosphere and application as vaccine adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Nanoparticles were prepared by an improved self-emulsifying solvent evaporation method (the schematic diagram of the preparation process is shown in figure 1 As shown, the schematic diagram of the prepared nanoparticles is shown in figure 2 shown), 100mg PLA and 30mg didodecyldimethylammonium bromide (DDAB) were dissolved in the oil phase mixed with ethanol and acetone, the volume of the oil phase was 15mL, where V 乙醇 :V 丙酮 1:1; then use deionized water as the water phase, the volume of the water phase is 90mL; then slowly add the well-mixed oil phase into the water phase, mechanically stir at a speed of 400rpm, stir for 12 hours, and finally centrifuge, Washing, freeze drying, the obtained polymer nanoparticles. Its average particle size and particle size distribution are measured by a British Malvern nanometer (ZetasizerNano ZS90). The average particle size in water is 117nm, the particle size distribution coefficient PDI is 0.138, and the surface Zeta potential is...

Embodiment 2

[0043] Nanoparticles were prepared by an improved self-emulsifying solvent evaporation method. 100mg PLA and 20mg didodecyldimethylammonium bromide (DDAB) were dissolved in an oil phase mixed with ethanol and acetone. The volume of the oil phase was 10mL, where V 乙醇 :V 丙酮 1:1; then use deionized water as the water phase, the volume of the water phase is 90mL; then slowly add the uniformly mixed oil phase into the water phase, mechanically stir at a speed of 400rpm, stir for 12 hours, and finally centrifuge, Wash and freeze-dry to obtain polymer nanoparticles. Its average particle size and particle size distribution are measured by a British Malvern nanometer (ZetasizerNano ZS90). The average particle size in water is 134.4nm, the particle size distribution coefficient PDI is 0.189, and the surface Zeta potential is 35.7mV. Scanning electron microscope photos as Figure 5 As shown, the results show that the prepared polymer nanoparticles have a uniform particle size.

Embodiment 3

[0045] Nanoparticles were prepared by an improved self-emulsifying solvent evaporation method. 100mg PLA and 50mg didodecyldimethylammonium bromide (DDAB) were dissolved in an oil phase mixed with ethanol and acetone. The volume of the oil phase was 15mL, where V 乙醇 :V 丙酮 1:1; then use deionized water as the water phase, the volume of the water phase is 90mL; then slowly add the uniformly mixed oil phase into the water phase, mechanically stir at a speed of 400rpm, stir for 12 hours, and finally centrifuge, Wash and freeze-dry to obtain polymer nanoparticles. Its average particle size and particle size distribution are measured by a British Malvern nanometer (ZetasizerNano ZS90). The average particle size in water is 177.7nm, the particle size distribution coefficient PDI is 0.209, and the surface Zeta potential is 35.5mV. Scanning electron microscope photos as Figure 6 As shown, the results show that the prepared polymer nanoparticles have a uniform particle size.

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Abstract

The invention relates to a polymer lipid nanoparticle, a preparation method and application thereof as a vaccine adjuvant system. The polymer nanoparticle is a solid sphere composed of a macromoleclar polymer, positively charged cationic lipid is inlaid on the surface, the nanoparticle surface can adsorb protein antigen, polypeptide antigen, viral antigen and other active drugs; the polymer nanoparticle has an average particle size of less than 300nm, a dispersion coefficient, i.e. a PDI (polydispersity) value of less than 0.2, and a Zeta potential average value of greater than 20mV. The polymer nanoparticle provided by the invention has the characteristics of uniform size, high antigen carrying rate, and good maintenance of antigen activity, when the polymer nanoparticle carries an antigen and serves as a vaccine adjuvant, the intake of antigen presenting cells (APCs) to the antigen can be increased, the activation level of antigen presenting cells is improved, and the follow-up immune response level is strengthened.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a polymer nanoparticle carrying protein antigens, polypeptide antigens, inactivated virus antigens and other active drugs, a preparation method thereof, and an application as an antigen delivery and adjuvant system. Background technique [0002] In recent years, drug delivery methods based on nanotechnology have attracted great attention and interest from the scientific and business communities. Nanoparticles have become a new type of drug or gene delivery carrier with great application prospects. There are many evidences that nanoparticles Drugs can be delivered to organs, tissues or cells in a targeted manner, which will provide new solutions for the treatment of malignant tumors, cardiovascular and cerebrovascular diseases, and nervous system diseases. Some nanoparticles can permeate through the physiological barrier through capillary, make the drug accumulate in specific par...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K9/19A61K9/127A61K39/00A61K47/34A61K39/39
Inventor 王连艳杨婷媛徐婧
Owner 王连艳
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