6results about How to "Increase secretion level" patented technology

The application belongs to the field of regenerative medicine and nano drug delivery system, and particularly relates to a drug-loaded exosome for improving the testosterone secretion capacity of testicular interstitial cells and a preparation method and application thereof. The application links a targeting FATE1 polypeptide to the surface of MSC-derived exosomes by a chemical coupling method, and loads a small molecule compound in the exosomes by electroporation, so as to obtain modified MSC-derived exosomes. The obtained exosomes can obtain the targeting capacity in testicular interstitial cells, and the natural repair capacity of MSC-derived exosomes and the release of small molecule drugs promote the repair of damaged cells, improve the testosterone secretion capacity of testicular interstitial cells, and relieve male erectile dysfunction caused by diseases or aging.

The application discloses application of a small-molecule compound targeting TIM-3 in tumor immunotherapy and relates to the technical field of immunology and antitumor drugs. The small-molecule compounds DTS and CT are safe and verified marketed drugs, and it is found that the small-molecule compounds can bind to TIM-3 protein and regulate T cell exhaustion related to TIM-3. + Experiments prove that DTS and CT can effectively inhibit CD8 T cell exhaustion, promote expansion of exhausted precursor T cells, and comprehensively enhance T cell antitumor immune function. In a melanoma model, the compounds significantly inhibit tumor growth and reduce the proportion of tumor-infiltrating T cell exhaustion. The application uses the strategy of "new use of old drugs", provides a TIM-3 small-molecule inhibitor with high safety, oral administration, strong penetration and low cost, effectively solves the clinical transformation predicament of existing antibody drugs and newly synthesized small molecules, and provides a new scheme for rapid transformation of tumor immunotherapy.

The invention belongs to the technical field of medicine. More specifically, the invention relates to application of telbivudine in preparation of drugs for preventing or treating breast cancer. The invention provides an application of telbivudine in preparation of a medicine for preventing or treating breast cancer. The invention proves that the telbivudine has an anti-breast cancer effect for the first time, and the telbivudine as the NSD2 inhibitor can effectively inhibit the growth of the breast cancer. Meanwhile, the telbivudine can remodel the immunosuppressive tumor microenvironment, reduce the infiltration of regulatory T cells (Treg), and significantly enhance the effector function of CD8 + T cells. More importantly, the telbivudine and the PD-1 antibody are combined to show a remarkable synergistic effect in a breast cancer mouse model, and the tumor inhibition effect and survival benefit are superior to those of a single drug. The combination scheme of the telbivudine and the PD-1 antibody significantly improves the sensitivity of breast cancer to immunotherapy, provides a new scheme for solving the problem of low clinical response rate of breast cancer immunotherapy, and has good application prospects and application values.

The invention relates to a method for improving the secretory expression level of expansin in bacillus subtilis, and aims to provide a method capable of promoting the bacillus subtilis to secrete expansin (microorganism-derived expansin BsEXLX1 and plant-derived expansin LeEXP2). The method comprises the following steps: firstly, screening a promoter capable of efficiently starting expansin to construct an engineering strain, and then performing overexpression of a secretory cofactor in the strain to improve the limitation of target protein in the aspects of secretory transport, folding maturation, stability and the like, thereby improving the extracellular secretion level of the two expansin. Amplification verification is carried out through a 5L fermentation tank, the secretion level of expansin is further improved, the expression quantity of BsEXLX1 protein is improved to 1.3 g / L, and the secretion quantity of LeEXP2 is improved to 43.7 mg / L. And a process basis and industrialization potential are provided for subsequent large-scale fermentation production and application development.

The technical scheme of the present application discloses a plastic film for providing high-quality light environment, which comprises the following components in parts by weight: 9,9-dianthraquinone 4-5 parts, pyrene anthrone 6-7.5 parts, anthracene-D10 4-6 parts, auxiliary agent 7.2-10.6 parts, and resin 70-80 parts. The preparation method comprises the following steps: S1, uniformly mixing 9,9-dianthraquinone, pyrene anthrone and anthracene-D10, heating to melting in an oxygen-free reaction kettle, cooling, and then crushing by a high-speed pulverizer to obtain a powder with a particle diameter of less than 100 nm; S2, mixing the powder with the auxiliary agent in a double-screw extruder, and then shearing and granulating to obtain a high-quality light environment additive master batch; and S3, mixing the high-quality light environment additive master batch with the resin, and preparing a plastic film by a flow casting and rolling process or a blowing process. The prepared plastic film effectively improves the photosynthetic rate of plants and significantly improves the quality and yield of plants.

This invention relates to a Kunitz-type potato trypsin inhibitor mutant, its preparation method, and its applications, belonging to the fields of genetic engineering and protein engineering modification technology. Based on a wild-type potato trypsin inhibitor, this invention constructs the mutant shown in SEQ ID NO.3. This mutant exhibits 5.67 times higher trypsin inhibitory activity than the wild type, and in animal models, it increases cholecystokinin (CCK) secretion levels by 1.94 times and reduces food intake by 44.58%. The Kunitz-type potato trypsin inhibitor mutant provided by this invention can serve as an active ingredient and has significant application potential in biomedicine and food health fields, particularly in regulating appetite and alleviating obesity.