2results about How to "Enhance antigen presentation" patented technology

The invention discloses an engineered adoptive macrophage based on a nano battery as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method disclosed by the invention comprises the following steps: taking an immunologic adjuvant working solution as a raw material, and obtaining nanoparticles with high drug loading capacity through an assembly effect; as the main energy of ChARM comes from an immunologic adjuvant, the high-drug-loading nano-particle is also called a high-energy-density nano-battery. Diluting the obtained nano particles with a cell culture medium, and co-incubating the diluted nano particles and macrophages to obtain engineered macrophages loaded with the anti-tumor or anti-inflammatory nano battery; the assembly mode is any one of supramolecular assembly, prodrug assembly or metal coordination assembly; the high-density drug-loaded nano battery can endow engineered cells with excellent biological functions. The method solves the technical problems that the preparation of the existing CAR-M is expensive, the gene modification is complex, and the nano-drug delivery system is unstable.

The application discloses an aptamer-based functionalized nano linker, a preparation method and application, wherein the functionalized nano linker comprises a functionalized nano vesicle as a carrier, the functionalized nano vesicle is a biological nano membrane vesicle prepared through combination treatment of an ICD functionalized biological membrane and a biological membrane carrying an azido group; part of the azido groups of the biological nano membrane vesicle are loaded with an aptamer I for targeting DC cells through alkyne-azido cycloaddition reaction, and the other part of the azido groups are loaded with an aptamer II for targeting T cells through alkyne-azido cycloaddition reaction. The application designs a dual-specific functionalized nano linker, which can simultaneously target and link DC and T cells, the functionalized nano vesicle as the carrier itself has key proteins of ICD effect, can promote maturation of the dendritic cells and antigen cross-presentation, after linking with the T cells, can effectively activate the CD8+ T cells and differentiate the CD8+ T cells to cytotoxicity effect phenotype, and enhance killing of tumor cells.