Highly pathogenic porcine reproductive and respiratory syndrome JXAl-R strain- porcine parvovirus disease bigeminal live vaccine and preparation method and application thereof

Abstract

The invention particularly relates to a highly pathogenic porcine reproductive and respiratory syndrome JXAl-R strain- porcine parvovirus disease bigeminal live vaccine and the preparation method and the application of the highly pathogenic porcine reproductive and respiratory syndrome JXAl-R strain-porcine parvovirus disease bigeminal live vaccine. The highly pathogenic porcine reproductive and respiratory syndrome JXAl-R strain- porcine parvovirus disease bigeminal live vaccine is the freeze-dried vaccine of mixed viral antigen liquid containing highly pathogenic porcine reproductive and respiratory syndrome virus and porcine parvovirus, the content of the highly pathogenic porcine reproductive and respiratory syndrome virus in per dose of the freeze-dried vaccine is 105-7 TCID50 / ml, the content of the porcine parvovirus virus is 105-7 TCID50 / ml, and the highly pathogenic porcine reproductive and respiratory syndrome JXAl-R strain- porcine parvovirus disease bigeminal live vaccine is prepared via the following steps: preparing highly pathogenic porcine reproductive and respiratory syndrome virus liquid, preparing porcine parvovirus liquid, matching the vaccines and freeze-drying the vaccine. The bigeminal live vaccine provided by the invention has excellent immunogenicity and safety, can prevent two epidemic diseases including the highly pathogenic porcine reproductive and respiratory syndrome and the porcine parvovirus disease simultaneously, thereby preventing two diseases by one injection.

Description

[0001] technical field [0002] The invention relates to the technical field of veterinary biological products, in particular to a highly pathogenic porcine reproductive and respiratory syndrome (JXA1-R strain)-porcine parvovirus disease dual live vaccine and its preparation method and application. [0003] Background technique [0004] Porcine reproductive and respiratory syndrome (PRRS), also known as blue ear disease, is a highly contagious infectious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRSV). Infection, but pregnant sows and piglets under 1 month of age are most susceptible. The disease is mainly characterized by miscarriage, stillbirth, weak fetus, mummified fetus, dyspnea, sepsis and high mortality in piglets. Highly pathogenic porcine blue-ear disease is an acute and highly lethal infectious disease caused by a mutant strain of porcine reproductive and respiratory syndrome virus. The main clinical manifestations are high b...

AI Technical Summary

Problems solved by technology

Because there is only a single vaccine available on the market at present, to prevent these two diseases at the same time, pigs of the same age group often need to be immunized with 2 injections, which increases the immune density of pigs of each age group, while the cost of vaccines and the cost of injection Manpower and stress on pigs, as well as short-term high-density injections cause mutual interference between vaccines, making pig farm operators miserable

With the development of intensive pig farming in China, traditional monovalent vaccines can no longer meet the needs of immunity, and there is an urgent need for vaccines that can prevent multiple diseases with one shot