192 results about "Infectious virus" patented technology

Methods for reprogramming primate somatic cells to pluripotency using an episomal vector that does not encode an infectious virus are disclosed. Pluripotent cells produced in the methods are also disclosed.

The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided.

A mutant herpesvirus that can be used a recombinant virus vector includes (a) a mutation such that the mutant virus has a reduced ability in comparison with a parent type to cause lysis of an infected cell, and (b) an inactivating mutation in a gene essential for the production of infectious virus. An example is a HSV1 mutant lacking the essential glycoprotein gH gene and having a mutation impairing the function of the gene product VP16. A heterologous gene can be carried at the site of the inactivated essential gene, e.g. a gene suitable for administering gene therapy. The vector has an increased margin of safety over known herpesvirus vectors in respect of incidence of cytopathic effects and/or risk of infection.

The invention relates to infectious clones of parvovirus B19, methods of cloning infectious B19 clones, and methods of cloning viral genomes that have secondary DNA structures that are unstable in bacterial cells. A B19 infectious clone and methods of producing B19 infectious clones are useful for producing infectious virus. Infectious virus is useful for identifying and developing therapeutically effective compositions for treatment and/or prevention of human parvovirus B19 infections.

Oncolytic viruses can be used to purge cellular compositions to remove undesired neoplastic cells before the cellular compositions are used for transplantation. The present invention relates to the use of a virus to pre-treat a subject prior to delivery into the subject a transplant that has been purged with the same virus. This pre-treatment serves to elicit an immune response in the subject against the virus, thereby protecting the subject from infections by the virus after receiving the transplant, which likely contains infectious viruses.

The invention belongs to the technical field of electronic cigarettes, and particularly relates to an electronic cigarette holder with an antibacterial layer. According to the technical scheme, a food-grade antibacterial agent for the infectious disease of digestive tract and the respiratory infectious virus or pathogenic bacteria wraps the electronic cigarette holder, and a medical slow release formulation is mixed in the antibacterial agent. The antibacterial layer of the cigarette holder specially conducts killing on the infectious disease of digestive tract and the respiratory infectious virus or pathogenic bacteria, wherein the infectious disease of digestive tract and the respiratory infectious virus or pathogenic bacteria are easily bred on residual saliva. The antibacterial agent can be appropriately and slowly released, the aim of timely killing the virus or the pathogenic bacteria can be achieved, and meanwhile the electronic cigarette holder has the better economical efficiency.

The present invention provides a composition comprising hepatitis B virus (HBV) component(s), and which may be either nucleic acid- or polypeptide-based as well as nucleic acid molecules and vectors encoding such HBV component(s). It also relates to infectious viral particles and host cells comprising such nucleic acid molecules or vectors. It also provides composition and kits of parts comprising such nucleic acid molecules, vectors, infectious viral particles or host cells and the therapeutic use thereof for preventing or treating HBV infections.

The invention discloses a method for detecting an FMDV (Foot and Mouth Disease Virus) group and O type and AsiaI type thereof through real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction). By carrying out specificity and sensitivity verification on the method established by the invention, the result shows that the FMDV can be well set apart from other Artiodactyla infectious viruses as well as O type and AsiaI type, and the sensitivity can be up to 10 copies of a template. The method established by the invention is characterized in that the whole reaction process is a closed pipe operation, thereby maximally reducing the pollution to the system. The whole process sequentially including the acquisition of a sample to be detected, the extraction of nucleic acid, the preparation of a fluorescent quantitative RT-PCR system and the representation of a reaction result can be completed within 2.5 hours, and the time required for detection is greatly shortened on the premise of ensuring the detection result.

The present invention provides novel compounds with activity against infectious viruses. The compounds of the invention may inhibit retroviral reverse transcriptases and thus inhibit the replication of the virus. They are useful for treating human patients infected with a human retrovirus, such as human immunodeficiency virus (strains of HIV-1 or HIV-2) or human T-cell leukemia viruses (HTLV-I or HTLV-II) which results in acquired immunodeficiency syndrome (AIDS) and/or related diseases. The present invention also relates generally to the accumulation or retention of therapeutic compounds inside cells. The invention is more particularly related to attaining high concentrations of active metabolite molecules in HIV infected cells. Intracellular targeting may be achieved by methods and compositions which allow accumulation or retention of biologically active agents inside cells. Such effective targeting may be applicable to a variety of therapeutic formulations and procedures.

The invention relates to a primer set and a kit for quickly detecting various melon viruses. By applying specificity primer pairs SEQ ID NO.1-10 designed according to nucleotide conserved region sequences of 5 viruses, a multiple RT-PCR system is optimized in aspects such as primer concentration, Mg2+ concentration, Taq DNA polymerase concentration, dNTPs concentration, annealing temperature and the like which influence multiple RT-PCR (M-PCR) amplification, and the primer set and a kit capable of simultaneously detecting field samples complexly infected by five viruses CMV, SqMV, TMV, WMV and ZYMV from watermelon leaf tissues are established. The primer set and the kit for detecting complex infectious viruses of melons have the advantages of saving time, reducing cost and improving efficiency.

The invention innovatively designs an air supply and ventilation protective mask. A miniature air supply fan is installed adopting a combined plug connection manner, and is externally connected with aportable mobile power supply to obtain electric power driving operation, the air in a mask shell is driven to flow, at the same time, the air impedance is overcome to the maximum extent, and multiple-layer or thickened air filters, namely the air inlet filter plate and the air outlet filter plate, is adapted to enhance the filtering protection function. Moreover, the mask shell does not directlycontact the face of a user, so that the user can breathe more smoothly and easily, moisture is taken away by the airflow, air filters away from the mouth nose of the user cannot be wetted, wearing ismore comfortable, and the protection is more safe and long-acting. According to the air supply and ventilation protective mask, by assembling an upper cover plate, a lower cover plate and goggles, theair filter plates are replaced and renewed to block the invasion of harmful air, substances or infectious viruses to the human body, and multiple safety and health protection are realized; and the air supply and ventilation protective mas can also be used by removing components such as the goggles, the air filters, sealing strips and the air supply fan. The air supply and ventilation protective mask has the characteristics of multi-scene application and reuse.

The present inventors developed 5a/2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and all of or part of NS2 were replaced by the corresponding genes of the genotype 5a reference strain SA13. Compared to the J6/JFH control virus, after transfection of in vitro transcripts in Huh7.5 cells, production of infectious viruses was delayed. However, in subsequent viral passages efficient spread of infection and HCV RNA titers as high as for J6/JFH were obtained. Infectivity titers were at all time points analyzed comparable to J6/JFH control virus. Sequence analysis of recovered 5a/2a recombinants from 2 serial passages and subsequent reverse genetic studies revealed adaptive mutations in p7, NS2 and/or NS3. Infectivity of the 5a/2a viruses was CD81 and SR-BI dependant, and the recombinant viruses could be neutralized by chronic phase sera from patients infected with genotype 5a. Conclusion: The developed 5a/2a viruses provide a robust in vitro tool for research in HCV genotype 5, including vaccine studies and functional analyses of an increasingly important genotype in South Africa and Europe.

The present invention is directed to a method of extracting virus, particularly reovirus, from a culture of cells. Infectious virus can be extracted from the culture with a detergent at a convenient temperature such as 25° C. or 37° C. to produce high virus titers. Both ionic and non-ionic detergents can be used in the present invention.

Described is a composition and method for reducing the occurrence and severity of infectious diseases, especially infectious diseases in which lipid-containing infectious viral organisms are found in biological fluids, such as blood. The present invention employs solvents useful for extracting lipids from the lipid-containing infectious viral organism thereby creating immunogenic modified, partially delipidated viral particles with reduced infectivity. The present invention provides delipidated viral vaccine compositions, such as therapeutic vaccine compositions, comprising these modified, partially delipidated viral particles with reduced infectivity, optionally combined with a pharmaceutically acceptable carrier or an immunostimulant. The vaccine composition is administered to a patient to provide protection against the lipid-containing infectious viral organism or, in case of a therapeutic vaccine, to treat or alleviate infection against the lipid-containing infections viral organism. The vaccine compositions of the present invention include combination vaccines of modified viral particles obtained from one or more strains of a virus and/or one or more types of virus.

The invention belongs to the field of precise medical research, and particularly relates to a HIV-1 protease detection method based on solid state nanopore. The HIV-1 protease is one of key enzymes in the copying process of HIV-1 viruses, can split protein precursor of virus particles, and make the viruses mature and become infectious virus particles. Successful detection of HIV-1 protease plays an important role in finding and treating AIDs. The HIV-1 protease detection method based on the solid state nanopore provides an identification method of the HIV-1 protease with a single molecule detection technology without marking and proliferating. The activity and the concentration of the HIV-1 protease are determined by detecting differences of protein signals formed after the process that target protein is enzyme digested by HIV-1 protease.

The invention discloses an intelligent meal delivery robot for hospital wards. The intelligent meal delivery robot comprises a robot body with a computer control device arranged on the upper end face,wherein the computer control device comprises a controller which is fixedly arranged on the upper end face of the robot body and provided with an intelligent chip; and a touch screen displayer capable of setting a meal delivery route for the robot through a touch screen is arranged on the front side end face of the controller, and a camera capable of carrying out face recognition on patients is arranged on the upper side of the touch screen displayer. According to the meal delivery robot, the meal delivery route can be planned and the identity of the meal takers can be identified through thecontroller 4 provided with the intelligent chip, and the controller controls a disinfection device to carry out disinfection processing on recovered meal delivery drawers, so that the situation that virus is spread out due to the fact that a patient carrying an infectious virus makes contact with meal delivery drawers is avoided, and virus spreading possibility is reduced.