15 results about "Infectious virus" patented technology

The invention discloses respiratory tract infectious virus prevention and treatment equipment which comprises an oral cavity part, a throat part and a chest part, the oral cavity part comprises a spherical infrared radiation shell, an electric heating layer is attached to the inner layer of the spherical infrared radiation shell, and a holding tube communicated with the interior of a spherical cavity is fixedly installed at the lower end of the spherical infrared radiation shell; the present invention provides a device for preventing and treating new coronal influenza, influenza A, pandemic influenza and recently found respiratory infectious viruses such as HMPV.

Provided are innovative compositions for tethering blocking an inactivating of airborne respiratory infectious viruses. The compositions comprise bispecific proteins with two different antigen binding regions (ABR), which are typically configured as immunoglobulin “single variable domains” (ISV). A first ISV binds to a surface protein found on an airborne infectious virus. A second ISV binds to a mucin protein, e.g. a mucin protein present on ocular, nasopharyngeal, tracheal and / or oral surfaces of a mammal. The two ISV are joined by a polypeptide linker.

Disclosed herein are synthetic VP1 proteins that contain engineered disulfide bonds. In some embodiments, the synthetic VP1 proteins with engineered disulfide bonds self-assemble into non-infectious virus-like particles (VLPs) that are more stable that non-infectious VLPs that resulted from self-assembly of VP1 proteins that lack engineered disulfide bonds.

The utility model relates to the technical field of medical instruments, in particular to a sickbed isolation device. The device comprises four groups of supporting mechanisms, isolating mechanisms and four groups of connecting mechanisms, the isolation mechanism comprises an isolation cover body covering the outer side of the sickbed, an isolation curtain fixedly arranged on one side in the isolation cover body so as to divide the isolation cover body into a first space and a second space, and an air filtering assembly arranged in the first space; the first fixing assembly is arranged between the ground and the isolation cover body in a sealed mode and used for fixing the isolation cover body, and the second fixing assembly is arranged between the ground and the isolation curtain in a sealed mode and used for fixing the isolation curtain. The device solves the problem that infectious viruses can be diffused to the outside air when medical staff enter and exit from the isolation assembly.

The invention relates to a recombinant chimeric antigen aiming at bovine nodular skin disease virus as well as a preparation method and application of the recombinant chimeric antigen. The bovine nodular skin disease virus recombinant chimeric antigen comprises four immunogens which are connected in series and fused according to a specific sequence: bovine nodular skin disease virus ORF122, ORF060, ORF074 and ORF117 proteins or antigenic fragments thereof, and can excite immune response aiming at two infectious virus particles, namely, intracellular mature virus particles (IMV) and extracellular membrane virus particles (EEV); therefore, the specific immune protection effect on the bovine nodular skin disease virus is efficiently excited; in addition, the bovine nodular skin disease virus vaccine disclosed by the invention also has good safety, quick responsiveness and productivity support, and has an extremely good clinical application prospect.

The application provides an avian leukosis virus multi-subtype antigen epitope fusion protein, a multi-subtype recognition antibody, a detection kit and application thereof. The amino acid sequence of the avian leukosis virus multi-subtype antigen epitope fusion protein is shown as SEQ ID NO:1. Experiments show that the avian leukosis virus multi-subtype antigen epitope fusion protein is reactive to avian leukosis virus multi-subtype serum and is not reactive to other common chicken infectious virus or bacterial serum. The avian leukosis virus multi-subtype antigen epitope fusion protein can be used for preparing multi-subtype recognition antibodies by immunizing animals. The multi-subtype recognition antibody can be specifically recognized with multi-subgroup avian leukosis specific antigen epitopes, a detection kit is developed, and the detection kit is used for rapid screening and purification of chicken population avian leukosis virus, and has a good application prospect.

The application provides application of tanshinone or a derivative thereof in preparation of a monkeypox virus resisting drug. In a cell model infected by the monkeypox virus, production of infectious virus particles can be significantly reduced. In a mouse infection model, Tanshinone VII can effectively reduce pathological damage of lung tissues and improve survival rate of animals. The above evidence indicates that Tanshinone VII has potential efficacy in resisting the monkeypox virus. It is inferred that tanshinone or the derivative thereof can play a double role in resisting the monkeypox virus and host protection.

A chromatography medium comprising porous beads having an inner porous core and an outer porous shell is used for chromatographic separation of enveloped or membranous biological particles from impurities such as contaminant DNA and / or protein. The core is capable of binding molecules via hydrophobic interactions; however, the pore size of the shell does not allow particles having a size of 20 nm and larger to permeate into the bead and interact with the core. The separation is performed at a pH of less than 7.4. The enveloped or membranous biological particles may have been subjected to a prior chromatographic capture step. When used for purification of enveloped virus particles the inventive process was found to yield a remarkably high rate of infectious virus particles.

The present invention relates to a label-free, high throughput method for determining the titer of an infectious virus in an aqueous sample comprising the infectious virus wherein the method comprises detecting the kinetics of morphological cell alterations, such as cell rounding, following virus infection, determining parameters defining a curve of morphological cell changes as a function of post-infection time, and determining the infectious virus titer based on a standard curve of the infectious virus at a known infection titer.

The invention discloses a method for quantitatively detecting cucumber green mottle mosaic virus (CGMMV) infectious viruses in watermelon seeds based on propionium bromide azide (PMA) pretreatment and reverse transcription quantitative polymerase chain reaction (RT-qPCR). The method comprises the following steps: S1, preparing a seed suspension containing virus particles; s2, grouping the suspensions, adding PMA with the final concentration of 150-170 [mu] M into a PMA treatment group, incubating for 20-40 min in a dark place, then carrying out blue light (460-490 nm) irradiation to enable PMA to selectively inhibit non-infectious virus nucleic acid amplification, and not adding PMA into a control group or carrying out inactivation treatment on the control group; s3, extracting nucleic acid, and carrying out RT-qPCR (real-time quantitative polymerase chain reaction) on the nucleic acid by adopting a specific primer pair CG-2439F / CG-2625R to obtain a Cq value or a nucleic acid amount; and S4, comparing the results of the PMA treatment group and the control group to determine the infectious CGMMV content or the live virus proportion. The method can reduce inactive virus interference, is suitable for seed quarantine, virus risk assessment and disinfection effect evaluation, and can provide a matched detection kit.

The present application belongs to the field of biomedical technology, and relates to a biological preparation capable of effectively inhibiting porcine delta coronavirus. The core effective component of the biological preparation is fly maggot protein peptide. The fly maggot protein peptide is prepared by short-time heat treatment of fly maggot protein, three-stage directional enzymolysis of alkaline protease-trypsin-flavor protease, 500-3000 Da membrane fractionation and weak cation exchange enrichment. Experimental results show that the fly maggot protein peptide can significantly reduce the N gene expression level, N protein expression level and infectious virus titer in PDCoV infected cells, has obvious direct inhibitory effect on PDCoV infection, and has certain preventive effect on infection. Compared with ordinary enzymolysis products, the 500-3000 Da cation peptide component obtained by the present application has significant anti-PDCoV activity, and can be used for preparing veterinary drugs or biological preparations for inhibiting PDCoV infection.

The application belongs to the field of pig infectious virus detection, and discloses a preparation method and application of a disposable electrochemical sensor for African swine fever virus (ASFV) based on in-situ loop-mediated isothermal amplification (LAMP). The current signal is collected by an electrochemical workstation, and a simple, economical and efficient method is provided for the diagnosis of ASFV. The steps are as follows: step 1, constructing a micro pool; step 2, preparing a modified electrode; step 3, constructing an electrochemical biosensor for detecting ASFV based on loop-mediated isothermal amplification technology; and step 4, electrochemically detecting ASFV. The electrochemical biosensor based on the LAMP strategy is combined with electrochemical detection to replace the traditional fluorescent sensing detection scheme, so that the entire detection system is miniaturized and simplified. In addition, the electrochemical detection method takes the current value as the signal output, has stable performance, realizes sensitive detection of ASFV, and further improves the sensitive detection by electrodepositing gold nanoparticles on the screen-printed electrode.

The present invention provides compositions, vaccines and methods for inducing protective immunity against an immunogen in humans. The protective immune response is obtained by using a saRNA, in particular a VRP vector as prime and a MVA for boost. Specifically, the present invention relates to genetically engineered (recombinant) VRP and MVA vectors comprising at least one heterologous nucleotide sequence encoding an antigenic determinant of an infectious virus such as EBV.

A method for preparing an infectious, recombinant virus vector comprises the steps of: (a) infecting host cells with a first virus comprising an encapsidation defective adenovirus (edAd), the edAd comprising a first defective virus genome; (b) incubating the infected host cells in a culture medium for a period of time sufficient for producing infectious virus particles; and (c) recovering infectious virus particles secreted into a culture supernatant, wherein the edAd or the host cells comprise a second defective virus genome engineered to express a target gene of interest, wherein the edAd or the host cells comprise nucleic acid sequences sufficient for expressing adenovirus (Ad) helper genes necessary for replication of the defective virus DNA; and wherein the edAd or the host cells comprise nucleic acid sequences sufficient from expressing helper functions necessary for producing infectious, replication defective virus particles corresponding to the second virus.

The invention discloses a method for detecting infectious norovirus in hot-processed seaweed, and belongs to the field of biotechnology and food safety detection. According to the detection method provided by the invention, long fragment RT-qPCR is carried out on a heat sensitive region (such as an RdRp region) in a targeted murine norovirus genome, and synergistic pretreatment of PMAxx / PtCl4 and PMA Enhancer is combined, so that infectious and non-infectious viruses in hot-processed algae are effectively distinguished, and particularly, under the condition of high-temperature treatment at 85-95 DEG C, the actual infection risk of the viruses can be reliably reflected and can reach 0.31 log TCID50 / 5g. The invention provides an accurate and reliable molecular detection tool for evaluating the virus inactivation effect of a seaweed heat processing technology (such as pasteurization, blanching and the like), and has important practical application value.