22 results about "Infectious virus" patented technology

The present invention relates to the technical field of disinfection for medical hyperbaric oxygen chambers. Disclosed is a sterilization and disinfection apparatus for a medical hyperbaric oxygen chamber. According to the present invention, a fixing plate is fixedly connected to a side of a fixing frame close to a fixing mechanism. A mounting plate is fixedly connected to a side of the fixing plate away from the fixing mechanism. Two sides of the mounting plate away from the fixing plate are fixedly connected to two ends of a disinfection lamp, respectively. A disinfection cylinder body is fixed to an air inlet end of an oxygen exhaust pipeline of the hyperbaric oxygen chamber. The disinfection lamp disinfects the gas flowing inside the pipeline, thereby effectively disinfecting and sterilizing the gas exhaled by patients in the hyperbaric oxygen chamber, which is then discharged into the atmosphere outside the chamber through the oxygen exhaust pipeline, avoiding the formation of a new virus source. The apparatus is designed to disinfect dedicated oxygen chambers for individuals infected with infectious viruses, avoiding the formation of a major viral contamination source, and preventing gas containing pathogens exhaled by patients from circulating inside the oxygen exhaust pipeline, thereby avoiding contamination of the pipelines inside the oxygen chamber.

The invention relates to a recombinant chimeric antigen aiming at bovine nodular skin disease virus, an immunogenic composition containing the recombinant chimeric antigen and application of the recombinant chimeric antigen. The bovine nodular skin disease virus recombinant chimeric antigen comprises two immunogens which are connected in series and fused according to a specific sequence: bovine nodular skin disease virus ORF122 and ORF060 proteins or antigenic fragments thereof, and can excite immune response aiming at two infectious virus particles, namely, intracellular mature virus particles (IMV) and cell outer enveloped virus particles (EEV); therefore, the specific immune protection effect on the bovine nodular skin disease virus is efficiently excited; in addition, the bovine nodular skin disease virus vaccine disclosed by the invention also has good safety, quick responsiveness and productivity support, and has an extremely good clinical application prospect.

The invention discloses respiratory tract infectious virus prevention and treatment equipment which comprises an oral cavity part, a throat part and a chest part, the oral cavity part comprises a spherical infrared radiation shell, an electric heating layer is attached to the inner layer of the spherical infrared radiation shell, and a holding tube communicated with the interior of a spherical cavity is fixedly installed at the lower end of the spherical infrared radiation shell; the present invention provides a device for preventing and treating new coronal influenza, influenza A, pandemic influenza and recently found respiratory infectious viruses such as HMPV.

Engineered, replication-deficient influenza viruses that are infectious and stimulate broadly-cross protective immunity against multiple different influenza strains have been developed. Compositions and methods for providing protective immune responses against influenza are provided. The methods deliver compositions of intact, replication-deficient influenza viruses by intradermal administration in an amount effective to elicit or stimulate a cross-protective immune response to influenza viruses in the recipient following a single administration. Because the engineered influenza viruses are non-replicating, they are safe and effective in immunocompromised subjects. Methods for delivering co-stimulatory molecules, growth factors, adjuvants and / or cytokines together with the non-replicating influenza viruses are also provided.

Provided are innovative compositions for tethering blocking an inactivating of airborne respiratory infectious viruses. The compositions comprise bispecific proteins with two different antigen binding regions (ABR), which are typically configured as immunoglobulin “single variable domains” (ISV). A first ISV binds to a surface protein found on an airborne infectious virus. A second ISV binds to a mucin protein, e.g. a mucin protein present on ocular, nasopharyngeal, tracheal and / or oral surfaces of a mammal. The two ISV are joined by a polypeptide linker.

Disclosed herein are synthetic VP1 proteins that contain engineered disulfide bonds. In some embodiments, the synthetic VP1 proteins with engineered disulfide bonds self-assemble into non-infectious virus-like particles (VLPs) that are more stable that non-infectious VLPs that resulted from self-assembly of VP1 proteins that lack engineered disulfide bonds.

The utility model relates to the technical field of medical instruments, in particular to a sickbed isolation device. The device comprises four groups of supporting mechanisms, isolating mechanisms and four groups of connecting mechanisms, the isolation mechanism comprises an isolation cover body covering the outer side of the sickbed, an isolation curtain fixedly arranged on one side in the isolation cover body so as to divide the isolation cover body into a first space and a second space, and an air filtering assembly arranged in the first space; the first fixing assembly is arranged between the ground and the isolation cover body in a sealed mode and used for fixing the isolation cover body, and the second fixing assembly is arranged between the ground and the isolation curtain in a sealed mode and used for fixing the isolation curtain. The device solves the problem that infectious viruses can be diffused to the outside air when medical staff enter and exit from the isolation assembly.

The invention relates to a recombinant chimeric antigen aiming at bovine nodular skin disease virus as well as a preparation method and application of the recombinant chimeric antigen. The bovine nodular skin disease virus recombinant chimeric antigen comprises four immunogens which are connected in series and fused according to a specific sequence: bovine nodular skin disease virus ORF122, ORF060, ORF074 and ORF117 proteins or antigenic fragments thereof, and can excite immune response aiming at two infectious virus particles, namely, intracellular mature virus particles (IMV) and extracellular membrane virus particles (EEV); therefore, the specific immune protection effect on the bovine nodular skin disease virus is efficiently excited; in addition, the bovine nodular skin disease virus vaccine disclosed by the invention also has good safety, quick responsiveness and productivity support, and has an extremely good clinical application prospect.

The application provides an avian leukosis virus multi-subtype antigen epitope fusion protein, a multi-subtype recognition antibody, a detection kit and application thereof. The amino acid sequence of the avian leukosis virus multi-subtype antigen epitope fusion protein is shown as SEQ ID NO:1. Experiments show that the avian leukosis virus multi-subtype antigen epitope fusion protein is reactive to avian leukosis virus multi-subtype serum and is not reactive to other common chicken infectious virus or bacterial serum. The avian leukosis virus multi-subtype antigen epitope fusion protein can be used for preparing multi-subtype recognition antibodies by immunizing animals. The multi-subtype recognition antibody can be specifically recognized with multi-subgroup avian leukosis specific antigen epitopes, a detection kit is developed, and the detection kit is used for rapid screening and purification of chicken population avian leukosis virus, and has a good application prospect.

The application provides application of tanshinone or a derivative thereof in preparation of a monkeypox virus resisting drug. In a cell model infected by the monkeypox virus, production of infectious virus particles can be significantly reduced. In a mouse infection model, Tanshinone VII can effectively reduce pathological damage of lung tissues and improve survival rate of animals. The above evidence indicates that Tanshinone VII has potential efficacy in resisting the monkeypox virus. It is inferred that tanshinone or the derivative thereof can play a double role in resisting the monkeypox virus and host protection.

A chromatography medium comprising porous beads having an inner porous core and an outer porous shell is used for chromatographic separation of enveloped or membranous biological particles from impurities such as contaminant DNA and / or protein. The core is capable of binding molecules via hydrophobic interactions; however, the pore size of the shell does not allow particles having a size of 20 nm and larger to permeate into the bead and interact with the core. The separation is performed at a pH of less than 7.4. The enveloped or membranous biological particles may have been subjected to a prior chromatographic capture step. When used for purification of enveloped virus particles the inventive process was found to yield a remarkably high rate of infectious virus particles.

The present invention relates to a label-free, high throughput method for determining the titer of an infectious virus in an aqueous sample comprising the infectious virus wherein the method comprises detecting the kinetics of morphological cell alterations, such as cell rounding, following virus infection, determining parameters defining a curve of morphological cell changes as a function of post-infection time, and determining the infectious virus titer based on a standard curve of the infectious virus at a known infection titer.

The present invention discloses an automatic dosing adjustment and sufficient flow-pushing device based on the AAO process, which belongs to the field of sewage treatment technology. It includes a sewage pretreatment tank and a biochemical tank, and the biochemical tank is sequentially divided into a disinfection tank, an anaerobic tank, an anoxic tank, and an aerobic tank; a water outlet is fixedly connected between the bottom of the disinfection tank and the anaerobic tank, the sewage pretreatment tank is fixedly connected to the biochemical tank, the right side of the aerobic tank is fixedly connected to a sewage pipe, the right end of the sewage pipe is fixedly connected to a sedimentation tank, a mud discharge pipe is provided at the bottom of the sedimentation tank, and a drain pipe is fixedly connected to the right side of the sedimentation tank; this improved AAO sewage treatment system realizes the linkage between the amount of drug added and the water level of the disinfection tank through a clever structural design. When the water level of the disinfection tank changes, the floating plate drives the top plate to act on the perforated rotating plate, thereby controlling the amount of drug outflow from the drug storage chamber. This design overcomes the defects of extensive operation in the field of urban sewage treatment, avoids the waste of drug consumption and energy consumption, and at the same time, can effectively guarantee the amount of disinfectant added, ensure the effective elimination of bacteria and viruses, and reduce the risk of transmission of infectious viruses.

The present invention relates to modular systems for vaccination against infectious agents that involves the delivery of, e.g., exosome-loaded, antigen-encoding mRNAs to and into cells and tissues of the immunized subject. The present invention also relates to compositions and methods for the design, preparation, manufacture, formulation, and / or use of vaccines, e.g., nucleic acid vaccines, loaded into extracellular vesicles, e.g., exosomes loaded with synthetic mRNAs encoding multiple surface and cytoplasmic antigens of interest, e.g., antigenic polypeptides derived from an infectious virus, e.g., SARS-CoV-2, designed to elicit strong humoral and cellular immune responses due to the simultaneous expression of antigens in their native state and as exosome-associated antigens.

To provide a pass box which is related to a technique when an article is delivered from the outside and carried in a chamber with controlled cleanliness in a medicine production plant, a precision apparatus factory and various research facilities, and in this case, the article to be carried in requires severe sterilization and dust removing functions due to being carried in a chamber with constant controlled cleanliness from ordinary atmosphere, and it is an essential condition that sterilization means to be used has no environmental load.SOLUTION: A pass box uses an LED11 enabling sterilization without environmental load, and has a function of sterilizing an article put in the bass pox by direct irradiation, and collecting, sterilizing and dust removing functions of germs by a highly efficient filter 9 due to circulation of air in a box body 1. The pass box can perform secure inactivation, sterilization and dust removal to virus with strong infectivity and general germs.SELECTED DRAWING: Figure 1

The invention discloses a method for quantitatively detecting cucumber green mottle mosaic virus (CGMMV) infectious viruses in watermelon seeds based on propionium bromide azide (PMA) pretreatment and reverse transcription quantitative polymerase chain reaction (RT-qPCR). The method comprises the following steps: S1, preparing a seed suspension containing virus particles; s2, grouping the suspensions, adding PMA with the final concentration of 150-170 [mu] M into a PMA treatment group, incubating for 20-40 min in a dark place, then carrying out blue light (460-490 nm) irradiation to enable PMA to selectively inhibit non-infectious virus nucleic acid amplification, and not adding PMA into a control group or carrying out inactivation treatment on the control group; s3, extracting nucleic acid, and carrying out RT-qPCR (real-time quantitative polymerase chain reaction) on the nucleic acid by adopting a specific primer pair CG-2439F / CG-2625R to obtain a Cq value or a nucleic acid amount; and S4, comparing the results of the PMA treatment group and the control group to determine the infectious CGMMV content or the live virus proportion. The method can reduce inactive virus interference, is suitable for seed quarantine, virus risk assessment and disinfection effect evaluation, and can provide a matched detection kit.

The present application belongs to the field of biomedical technology, and relates to a biological preparation capable of effectively inhibiting porcine delta coronavirus. The core effective component of the biological preparation is fly maggot protein peptide. The fly maggot protein peptide is prepared by short-time heat treatment of fly maggot protein, three-stage directional enzymolysis of alkaline protease-trypsin-flavor protease, 500-3000 Da membrane fractionation and weak cation exchange enrichment. Experimental results show that the fly maggot protein peptide can significantly reduce the N gene expression level, N protein expression level and infectious virus titer in PDCoV infected cells, has obvious direct inhibitory effect on PDCoV infection, and has certain preventive effect on infection. Compared with ordinary enzymolysis products, the 500-3000 Da cation peptide component obtained by the present application has significant anti-PDCoV activity, and can be used for preparing veterinary drugs or biological preparations for inhibiting PDCoV infection.

The application belongs to the field of pig infectious virus detection, and discloses a preparation method and application of a disposable electrochemical sensor for African swine fever virus (ASFV) based on in-situ loop-mediated isothermal amplification (LAMP). The current signal is collected by an electrochemical workstation, and a simple, economical and efficient method is provided for the diagnosis of ASFV. The steps are as follows: step 1, constructing a micro pool; step 2, preparing a modified electrode; step 3, constructing an electrochemical biosensor for detecting ASFV based on loop-mediated isothermal amplification technology; and step 4, electrochemically detecting ASFV. The electrochemical biosensor based on the LAMP strategy is combined with electrochemical detection to replace the traditional fluorescent sensing detection scheme, so that the entire detection system is miniaturized and simplified. In addition, the electrochemical detection method takes the current value as the signal output, has stable performance, realizes sensitive detection of ASFV, and further improves the sensitive detection by electrodepositing gold nanoparticles on the screen-printed electrode.

The invention discloses an NDV (Newcastle Disease Virus), AIV (Avian Infectious Virus) and IBV (Infectious Bursal Virus) triple rapid detection method based on a micro-fluidic chip and an LAMP (Loop-Mediated Isothermal The method comprises the following steps: (1) preparing a working solution from LAMP primers designed for NDV, AIV and IBV according to a specific final concentration, embedding the working solution in a micro-fluidic chip reaction chamber, and drying and fixing the working solution; (2) mixing a nucleic acid sample to be detected with the reaction liquid, adding the mixture into a chip sample adding hole, sealing and removing bubbles; and (3) pressing the reaction liquid into each reaction chamber through centrifugation, carrying out LAMP amplification for 30 minutes at a constant temperature of 63.5 DEG C, and collecting fluorescence signals in real time during the amplification so as to realize rapid detection of the target virus. According to the present invention, the highly integrated design of the microfluidic chip and the efficient, rapid and constant temperature characteristics of the LAMP amplification technology are integrated so as to achieve the synchronous, rapid and sensitive detection of the three important poultry respiratory viruses;

The present invention provides compositions, vaccines and methods for inducing protective immunity against an immunogen in humans. The protective immune response is obtained by using a saRNA, in particular a VRP vector as prime and a MVA for boost. Specifically, the present invention relates to genetically engineered (recombinant) VRP and MVA vectors comprising at least one heterologous nucleotide sequence encoding an antigenic determinant of an infectious virus such as EBV.

A method for preparing an infectious, recombinant virus vector comprises the steps of: (a) infecting host cells with a first virus comprising an encapsidation defective adenovirus (edAd), the edAd comprising a first defective virus genome; (b) incubating the infected host cells in a culture medium for a period of time sufficient for producing infectious virus particles; and (c) recovering infectious virus particles secreted into a culture supernatant, wherein the edAd or the host cells comprise a second defective virus genome engineered to express a target gene of interest, wherein the edAd or the host cells comprise nucleic acid sequences sufficient for expressing adenovirus (Ad) helper genes necessary for replication of the defective virus DNA; and wherein the edAd or the host cells comprise nucleic acid sequences sufficient from expressing helper functions necessary for producing infectious, replication defective virus particles corresponding to the second virus.

The invention discloses a method for detecting infectious norovirus in hot-processed seaweed, and belongs to the field of biotechnology and food safety detection. According to the detection method provided by the invention, long fragment RT-qPCR is carried out on a heat sensitive region (such as an RdRp region) in a targeted murine norovirus genome, and synergistic pretreatment of PMAxx / PtCl4 and PMA Enhancer is combined, so that infectious and non-infectious viruses in hot-processed algae are effectively distinguished, and particularly, under the condition of high-temperature treatment at 85-95 DEG C, the actual infection risk of the viruses can be reliably reflected and can reach 0.31 log TCID50 / 5g. The invention provides an accurate and reliable molecular detection tool for evaluating the virus inactivation effect of a seaweed heat processing technology (such as pasteurization, blanching and the like), and has important practical application value.