26 results about "Viral contamination" patented technology

The present invention relates to the technical field of disinfection for medical hyperbaric oxygen chambers. Disclosed is a sterilization and disinfection apparatus for a medical hyperbaric oxygen chamber. According to the present invention, a fixing plate is fixedly connected to a side of a fixing frame close to a fixing mechanism. A mounting plate is fixedly connected to a side of the fixing plate away from the fixing mechanism. Two sides of the mounting plate away from the fixing plate are fixedly connected to two ends of a disinfection lamp, respectively. A disinfection cylinder body is fixed to an air inlet end of an oxygen exhaust pipeline of the hyperbaric oxygen chamber. The disinfection lamp disinfects the gas flowing inside the pipeline, thereby effectively disinfecting and sterilizing the gas exhaled by patients in the hyperbaric oxygen chamber, which is then discharged into the atmosphere outside the chamber through the oxygen exhaust pipeline, avoiding the formation of a new virus source. The apparatus is designed to disinfect dedicated oxygen chambers for individuals infected with infectious viruses, avoiding the formation of a major viral contamination source, and preventing gas containing pathogens exhaled by patients from circulating inside the oxygen exhaust pipeline, thereby avoiding contamination of the pipelines inside the oxygen chamber.

The invention provides a qPCR (quantitative polymerase chain reaction) primer probe group for detecting various nodaviruses, a detection method and application, and belongs to the technical field of molecular biological detection. Aiming at various nodaviruses capable of infecting insect cell lines, including animal shed virus, Tn5 cell line virus, black beetle virus, borawa virus, nodavirus and macrobrachium rosenbergii nodavirus, a set of primer probe combination is designed. In the combination, part of viruses share the upstream primer and the probe, so that synchronous detection and identification of multiple target viruses in one reaction system are realized. The invention also provides a kit containing the primer probe group and a detection method. The detection method provided by the invention has the advantages of wide coverage range, high detection sensitivity, strong specificity, good matrix interference resistance and the like, and is suitable for safety detection and quality control of nodavirus pollution in insect cell banks, cell cultures and related biological products thereof.

This application provides a rhabdovirus-free Sf9-PT cell line and its applications. This cell line was deposited on March 15, 2023, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: C202334. The Sf9-PT cell line screened in this application is free from rhabdovirus contamination, allows for high-density culture, exhibits higher viral proliferation levels, and demonstrates more durable viral packaging stability. This cell line can serve as a modified alternative host for the development and commercial production of biological products and vaccines, and can more safely avoid the potential dangers posed by Sf-RV virus contamination.

The present invention provides a mammalian cell line that has been genetically engineered to make it resistant to viral entry and / or replication; and provides a method for using the cell line to reduce or prevent viral contamination of a bioproduction system.

The present invention relates to the technical field of virus detection, and discloses a sewage diarrhea virus typing detection system, which comprises a pathogen enrichment unit, an improved polyethylene glycol precipitation method is adopted to carry out pathogen enrichment on a sewage sample; and the nucleic acid extraction unit is used for extracting nucleic acid in the enriched sample by using a magnetic bead extraction method. According to the typing detection system for the diarrhea viruses in the sewage, simultaneous detection and typing of various diarrhea viruses such as norovirus, rotavirus, adenovirus, astrovirus, parareovirus and fiveleaf virus in the sewage can be realized, the detection range is widened, powerful support is provided for comprehensive monitoring of virus pollution conditions in the sewage, and the system is suitable for popularization and application. According to the present invention, the multiple fluorescent RT-PCR technology is adopted, and the efficient sample enrichment and nucleic acid extraction method is combined, such that the detection sensitivity is improved, the low-concentration viral nucleic acid can be detected, and the potential viral threat is not missed;

This invention provides a primer-probe set, detection method, and application for qPCR detection of various nodaviruses, belonging to the field of molecular biology detection technology. This invention designs a primer-probe combination for various nodaviruses that can infect insect cell lines, including farm virus, Tn5 cell line virus, black beetle virus, Boralwa virus, Nodamura virus, and Macrobrachium rosenbergii nodavirus. In this combination, some viruses share upstream primers and probes, enabling simultaneous detection and identification of multiple target viruses in a single reaction system. This invention also provides a kit and detection method containing this primer-probe set. The detection method of this invention has advantages such as wide coverage, high detection sensitivity, strong specificity, and good resistance to matrix interference, and is suitable for the safety detection and quality control of nodavirus contamination in insect cell banks, cell cultures, and related biological products.

The present application provides a preparation method of human serum albumin, which comprises inoculating human serum albumin-producing Pichia pastoris into a culture medium for fermentation, and obtaining human serum albumin from the fermentation liquor; the culture medium is BSM culture medium with the concentration of all components reduced to at most 1 / 4 except for two components of glycerol and Pichia pastoris trace elements 1, preferably reduced to 1 / 4-1 / 2. The present application also provides a purification method of human serum albumin. The yield and the proportion of human serum albumin in total protein obtained by the preparation method of the present application are significantly higher than those of the prior art, the cost of the culture medium used in the preparation method is significantly reduced, and there is no risk of animal-derived virus contamination, thereby significantly reducing the cost of the preparation method. The recovery rate is significantly improved by using the purification method of the present application, and the purity of the final product is also significantly further improved.

Provided herein are secretory compositions / cocktails derived from a novel, extremophilic fungal species cultured, isolated, and characterized herein. Said novel, extremophilic fungal species may be exploited to produce secretory compositions / cocktails which comprise a unique mixture of thermo-tolerant, stable enzymes capable of digesting cellular and genetic material in its surroundings under extreme temperatures. Such secretory compositions / cocktails may be useful as cleaning and sterilization solutions against biofilm, urine, yeast, bacterial, or viral contamination. Other uses include, but are not limited to, urea removal, fertilizer wastewaters, wastewater reclamation for life support systems in space, pH control, source of ammonia and carbon dioxide, starch digestion, biofuel production, as pharmaceuticals, and medical treatments such as wounds, skin disorders, or nail disorders.

The invention provides a sheep embryo fibroblast line and application thereof, and belongs to the technical field of cell biology. The name of the sheep embryo fibroblast line is sheep embryo fibroblast OEF, the sheep embryo fibroblast line is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is January 26, 2022, and the preservation number is CCTCC NO: C202212. The sheep embryo fibroblast line can be used for separation and culture of bovine and sheep pox viridae viruses such as LSDV, GTPV, SPPV and ORFV, has the characteristics of short proliferation time and high virus titer, and can avoid the risk that other viruses are polluted by viruses generated by primary cell separation.

The present disclosure is directed to a streamlined sample preparation process, VERA (Viral Enrichment by Reducing Artifacts), to tilt total genomic material in favor of DNA / RNA viral genomes. This reduction of host genomic artifacts can be completed in <8 hours from sample acquisition. Using a rapid library preparation protocol (˜1.5 minutes) and real-time nanopore sequencing, potential viral contamination, for example RNA viral contamination, can be identified in less than one workday from sample acquisition.

Invention is described which sterilizes air contaminated with airborne virus by means of superheating the air to over 400 Fahrenheit then cooling air to breathable temperatures. The invention disposes a fan pushing contaminated air into a positive temperature coefficient semiconductor heater to superheat the air which is subsequently cooled by a two stage cooling system. First stage of the cooling system is an air-to-air heat exchanger using ambient air from second fan to cool the superheated air. The second stage of cooling is provided by a solid-state Peltier heat pump which further transfers heat energy to ambient air provided by a third fan. Invention is disposed onto a sealed helmet which the sterilized air is conveyed causing an overpressure to eliminate the possibility of contaminated air entering the helmet. The invention is entirely reusable after use in a contaminated environment and does not rely on filters to remove airborne virus.

The invention belongs to the technical field of medical cosmetology and tissue regeneration, and particularly relates to a platelet-rich plasma vacuum blood collection tube containing biotin and recombinant humanized XVII type collagen as well as a preparation method and application of the platelet-rich plasma vacuum blood collection tube. Separating the glue layer; an anticoagulant solution layer; the interior of the tube body is vacuumized, and the preset sequence of the functional solution layer, the separation gel layer and the anticoagulant solution layer is configured as follows: after blood is introduced and centrifuged, biotin and recombinant XVII type collagen can migrate from the bottom of the tube and are specifically compounded in a platelet rich plasma (PRP) layer above the separation gel layer; and forming a PRP composite liquid layer different from the red blood cell layer, the separation gel layer and the plasma layer. According to the invention, the time is saved, the pollution risk is reduced, the risks of immunological rejection and disease transmission are avoided, the immunogenicity and virus pollution risk are overcome, the stability and quality uniformity are ensured, and the method accords with the production specifications of medical apparatuses and instruments.

The present disclosure is directed to a streamlined sample preparation process, VERA (Viral Enrichment by Reducing Artifacts), to tilt total genomic material in favor of DNA / RNA viral genomes. This reduction of host genomic artifacts can be completed in <8 hours from sample acquisition. Using a rapid library preparation protocol (˜15 minutes) and real-time nanopore sequencing, potential viral contamination, for example RNA viral contamination, can be identified in less than one workday from sample acquisition.

The invention provides a stirring type reactor culture process of a porcine circovirus type 2 recombinant baculovirus, which comprises the following steps: preparing a seed virus of the porcine circovirus type 2 recombinant baculovirus by using an insect cell Sf9 full-suspension culture process, and inoculating an insect cell High Five by using the seed virus prepared by using an Sf9 cell to perform antigen expression of the porcine circovirus type 2 recombinant baculovirus. A full-suspension culture mode is utilized, so that the labor intensity is reduced, the production cost is reduced, and pollution is not easily caused; 2) the risk of porcine virus pollution can be reduced by using insect cells and a baculovirus expression system; and 3) the characteristics (Sf9 and High Five) of different insect cells are simultaneously utilized to respectively carry out seed virus preparation and antigen expression, so that higher virus titer can be obtained, the virus titer is greater than or equal to 7.50 Log (TCID50 / mL), higher recombinant protein expression is obtained, and the protein expression quantity is greater than or equal to 150 mu g / mL.

To develop a stable liquid formulation of a persistent conjugate of a peptide active against all developed glucagon receptors, GLP-1 receptors, and GIP receptors, which can be stored for extended periods without concerns about viral contamination. [Solution] The present invention relates to a liquid formulation of a sustained-release conjugate of glucagon, GLP-1, and GIP triple-active compound, and a method for producing the same.

This invention discloses a chicken embryo fibroblast, its preparation method, and its application, relating to the field of biopharmaceutical technology. The preparation method of the chicken embryo fibroblast includes the following steps: S1, providing chicken embryos and obtaining the muscle tissue of the chicken embryos; S2, mixing the muscle tissue with a plant-derived compound digestion solution, and sequentially performing digestion, purification, and primary culture treatment using a serum-free special culture medium to obtain primary chicken embryo fibroblasts; wherein, the plant-derived compound digestion solution includes bromelain and papain. This invention, using a plant-derived compound digestion solution and a serum-free special culture medium, significantly improves the batch-to-batch stability, cell yield, and cell purity of the cells, reducing the risk of exogenous viral contamination. Therefore, the prepared chicken embryo fibroblasts exhibit high batch-to-batch stability and good reproducibility in viral culture.

This invention belongs to the field of virus detection technology, specifically providing a primer and probe composition for the simultaneous detection of mouse parvovirus (MVM) and mouse thymvirus (MTLV), including primers and probes for detecting MVM and MTLV respectively. This invention also provides a kit comprising the above-mentioned primer and probe composition, and a method for the simultaneous detection of MVM and MTLV. The primer and probe composition, kit, and method provided by this invention can specifically amplify both MVM and MTLV viruses without cross-reacting with other viral nucleic acids or with the genomes of various commonly used cell lines that may be present in the sample. It exhibits good specificity, high sensitivity, and good reproducibility, overcoming the shortcoming of existing technologies that require separate detection of MVM and MTLV, and providing an excellent detection method for the prevention and control of exogenous viral contamination in rodent biological products.

The invention discloses an NK cell serum-free culture solution and a culture method of NK cells. The NK cell serum-free culture solution comprises a basic matrix, a multi-factor slow-release carrier and a function regulation component, the basic matrix comprises recombinant human serum albumin, recombinant human transferrin, plant-derived lipidosome, 12 core recombinant proteins and 5 micromolecular nutrient substances, and the plant-derived lipidosome is composed of soybean phospholipid and linolenic acid; the multi-factor slow-release carrier is amination modified mesoporous silicon nanoparticles, the amination modified mesoporous silicon nanoparticles load four types of functional factors, the culture solution adopts recombinant human-derived components and plant-derived lipidosome to construct a basic matrix, animal serum dependence is thoroughly abandoned, and the culture solution is a novel culture solution. The serum batch difference, virus pollution and heterogeneous protein sensitization risks are effectively avoided, meanwhile, the concentration of each component is accurate and controllable, and the batch-to-batch consistency and quality stability of the culture solution are remarkably improved.

Doubly reentrant topology (DRT) is a unique structure. The present invention first validated the outstanding performance of an anti-biofouling artificial surface comprising a superhydrophobic surface; thereon a plurality of microstructures and having a doubly re-entrant topology (DRT) situated atop respective base structures which demonstrates a striking anti-biofouling effect that can prevent viral contamination. Furthermore, the present invention per se features excellent anti-biofouling ability, which may shed light on the applications of pathogen elimination in alleviating the COVID-19 pandemic.

The application relates to a high-virus-removal-rate fiber virus-removal membrane, comprising a porous body, the porous body having non-directional winding channels, the two sides of the porous body being respectively a first outer surface and a second outer surface, the porous body comprising a pre-filter layer and a separation layer, the separation layer being located on the side of the pre-filter layer close to the second outer surface, and the pre-filter layer and the separation layer being connected by a continuous fiber; the SEM-measured average pore diameter of the pre-filter layer is larger than that of the separation layer; the thickness of the separation layer is 5-60 mu m, and the SEM-measured average pore diameter of the separation layer is 25-85 nm; for PP7 phages, the top washing LRV of the virus-removal membrane is not less than 4, and the ratio of the top washing LRV of the virus-removal membrane to the initial LRV is not less than 0.7. The application further discloses a preparation process of the virus-removal membrane. The fiber virus-removal membrane has a high protein yield, and has a high virus-removal effect before and after top washing, so that the virus pollution risk can be reduced, and the economic benefit can be improved.

Methods for reducing the risk of viral infection in animals due to consumption of viral contaminated feed. The methods comprise administering to an animal in need thereof an effective amount of a feed additive, wherein the feed additive comprises a mixture of organic acids. The methods can be used to reduce the risk of infection in animals whose feed may or may not be contaminated with certain viruses.

There is provided inexpensive means capable of exhibiting an inactivation effect on microorganisms without assuming the presence of light while minimizing the burden on the environment.Bacteria or viruses are inactivated by bringing a compound having a redox mechanism below into contact with a target suspected to be contaminated by bacteria or viruses:in the formula, X− represents a counteranion, and a broken line represents a bonding position with another atom.

A packaging system for a coronavirus pseudovirus, including a vesicular stomatitis virus (VSV) vector in which Fluc and EGFP dual-reporter genes replace a GP gene, and packaging cell that expresses a coronavirus spike protein. The packaging system may quickly package pseudoviruses by using a one-step packaging method, and may be used in the research of coronaviruses such as COVID-19 (SARS-CoV-2), SARS (SARS-CoV) and MERS, and other viruses. The packaging system and thereby pseudovirus method may also be used to evaluate the efficacy of disinfectants by means of virus contamination distribution models, setting up scenarios, and sampling and testing steps.

The invention belongs to the field of biology, and particularly relates to a PK-15 suspension cell line capable of efficiently breeding porcine circovirus and hog cholera virus, the PK-15 suspension cell line is proposed to be named as a porcine kidney cell suspension cell line in taxonomy, is preserved in Guangdong Microbial Culture Collection Center, has a preservation number of GDMCC NO.66284, is preserved on May 19, 2025, and has a preservation number of CGMCC NO.66284. The PK-15 suspension cell line can be used for efficiently breeding porcine circovirus and hog cholera virus. The preservation address is the fifth floor of building 59, No.100 courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province. The cell line is obtained through domestication by a suspension method originally created by the applicant, is named as PK15-WS04, and is preserved in Guangdong Microbial Culture Collection Center. After the PK-15 suspension cell line is subjected to 50 generations of passage, cells of each generation grow aseptically and are free of exogenous virus pollution, and the sensitivity of the PK-15 suspension cell line to porcine circovirus and hog cholera virus is not reduced; the immunogenicity of the virus prepared by each generation of cells is stable; the cell line can be used as a reserve cell line for vaccine production. Meanwhile, the invention also discloses a related application of the PK-15 suspension cell line.

This invention discloses a method for preparing and applying cell vesicles derived from the rhizomes of traditional Chinese medicinal plants. The preparation method includes the following steps: washing and crushing the rhizomes of the medicinal plants, adding PBS buffer and grinding to obtain a homogenate; centrifuging the homogenate differentially and collecting the supernatant; subjecting the supernatant to a first ultracentrifugation to obtain a crude extract of cell vesicles; resuspending the crude extract of cell vesicles and loading it into a discontinuous sucrose density gradient solution for a second ultracentrifugation; collecting gradient bands with buoyant densities between 1.13 g / mL and 1.19 g / mL. This invention provides a stable and scalable preparation process that, through differential centrifugation combined with sucrose density gradient ultracentrifugation, can efficiently separate high-purity, structurally intact cell vesicles from the rhizomes of traditional Chinese medicinal plants such as Bletilla striata, Polygonatum sibiricum, and Curculigo orchioides. The process has good reproducibility and avoids the ethical and viral contamination risks associated with mammalian-derived vesicles.

Provided herein is a method of treating a material contaminated with a virus, the method comprising exposing the material to a vapor, wherein the vapor is produced by heating and / or vaporizing a dry cleaning solvent, and wherein the method at least partially inactivates the virus.