15 results about "Viral contamination" patented technology

This application provides a rhabdovirus-free Sf9-PT cell line and its applications. This cell line was deposited on March 15, 2023, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: C202334. The Sf9-PT cell line screened in this application is free from rhabdovirus contamination, allows for high-density culture, exhibits higher viral proliferation levels, and demonstrates more durable viral packaging stability. This cell line can serve as a modified alternative host for the development and commercial production of biological products and vaccines, and can more safely avoid the potential dangers posed by Sf-RV virus contamination.

The present invention provides a mammalian cell line that has been genetically engineered to make it resistant to viral entry and / or replication; and provides a method for using the cell line to reduce or prevent viral contamination of a bioproduction system.

This invention provides a primer-probe set, detection method, and application for qPCR detection of various nodaviruses, belonging to the field of molecular biology detection technology. This invention designs a primer-probe combination for various nodaviruses that can infect insect cell lines, including farm virus, Tn5 cell line virus, black beetle virus, Boralwa virus, Nodamura virus, and Macrobrachium rosenbergii nodavirus. In this combination, some viruses share upstream primers and probes, enabling simultaneous detection and identification of multiple target viruses in a single reaction system. This invention also provides a kit and detection method containing this primer-probe set. The detection method of this invention has advantages such as wide coverage, high detection sensitivity, strong specificity, and good resistance to matrix interference, and is suitable for the safety detection and quality control of nodavirus contamination in insect cell banks, cell cultures, and related biological products.

Provided herein are secretory compositions / cocktails derived from a novel, extremophilic fungal species cultured, isolated, and characterized herein. Said novel, extremophilic fungal species may be exploited to produce secretory compositions / cocktails which comprise a unique mixture of thermo-tolerant, stable enzymes capable of digesting cellular and genetic material in its surroundings under extreme temperatures. Such secretory compositions / cocktails may be useful as cleaning and sterilization solutions against biofilm, urine, yeast, bacterial, or viral contamination. Other uses include, but are not limited to, urea removal, fertilizer wastewaters, wastewater reclamation for life support systems in space, pH control, source of ammonia and carbon dioxide, starch digestion, biofuel production, as pharmaceuticals, and medical treatments such as wounds, skin disorders, or nail disorders.

The invention provides a sheep embryo fibroblast line and application thereof, and belongs to the technical field of cell biology. The name of the sheep embryo fibroblast line is sheep embryo fibroblast OEF, the sheep embryo fibroblast line is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is January 26, 2022, and the preservation number is CCTCC NO: C202212. The sheep embryo fibroblast line can be used for separation and culture of bovine and sheep pox viridae viruses such as LSDV, GTPV, SPPV and ORFV, has the characteristics of short proliferation time and high virus titer, and can avoid the risk that other viruses are polluted by viruses generated by primary cell separation.

The present disclosure is directed to a streamlined sample preparation process, VERA (Viral Enrichment by Reducing Artifacts), to tilt total genomic material in favor of DNA / RNA viral genomes. This reduction of host genomic artifacts can be completed in <8 hours from sample acquisition. Using a rapid library preparation protocol (˜15 minutes) and real-time nanopore sequencing, potential viral contamination, for example RNA viral contamination, can be identified in less than one workday from sample acquisition.

To develop a stable liquid formulation of a persistent conjugate of a peptide active against all developed glucagon receptors, GLP-1 receptors, and GIP receptors, which can be stored for extended periods without concerns about viral contamination. [Solution] The present invention relates to a liquid formulation of a sustained-release conjugate of glucagon, GLP-1, and GIP triple-active compound, and a method for producing the same.

This invention discloses a chicken embryo fibroblast, its preparation method, and its application, relating to the field of biopharmaceutical technology. The preparation method of the chicken embryo fibroblast includes the following steps: S1, providing chicken embryos and obtaining the muscle tissue of the chicken embryos; S2, mixing the muscle tissue with a plant-derived compound digestion solution, and sequentially performing digestion, purification, and primary culture treatment using a serum-free special culture medium to obtain primary chicken embryo fibroblasts; wherein, the plant-derived compound digestion solution includes bromelain and papain. This invention, using a plant-derived compound digestion solution and a serum-free special culture medium, significantly improves the batch-to-batch stability, cell yield, and cell purity of the cells, reducing the risk of exogenous viral contamination. Therefore, the prepared chicken embryo fibroblasts exhibit high batch-to-batch stability and good reproducibility in viral culture.

This invention belongs to the field of virus detection technology, specifically providing a primer and probe composition for the simultaneous detection of mouse parvovirus (MVM) and mouse thymvirus (MTLV), including primers and probes for detecting MVM and MTLV respectively. This invention also provides a kit comprising the above-mentioned primer and probe composition, and a method for the simultaneous detection of MVM and MTLV. The primer and probe composition, kit, and method provided by this invention can specifically amplify both MVM and MTLV viruses without cross-reacting with other viral nucleic acids or with the genomes of various commonly used cell lines that may be present in the sample. It exhibits good specificity, high sensitivity, and good reproducibility, overcoming the shortcoming of existing technologies that require separate detection of MVM and MTLV, and providing an excellent detection method for the prevention and control of exogenous viral contamination in rodent biological products.

The invention discloses an NK cell serum-free culture solution and a culture method of NK cells. The NK cell serum-free culture solution comprises a basic matrix, a multi-factor slow-release carrier and a function regulation component, the basic matrix comprises recombinant human serum albumin, recombinant human transferrin, plant-derived lipidosome, 12 core recombinant proteins and 5 micromolecular nutrient substances, and the plant-derived lipidosome is composed of soybean phospholipid and linolenic acid; the multi-factor slow-release carrier is amination modified mesoporous silicon nanoparticles, the amination modified mesoporous silicon nanoparticles load four types of functional factors, the culture solution adopts recombinant human-derived components and plant-derived lipidosome to construct a basic matrix, animal serum dependence is thoroughly abandoned, and the culture solution is a novel culture solution. The serum batch difference, virus pollution and heterogeneous protein sensitization risks are effectively avoided, meanwhile, the concentration of each component is accurate and controllable, and the batch-to-batch consistency and quality stability of the culture solution are remarkably improved.

The application relates to a high-virus-removal-rate fiber virus-removal membrane, comprising a porous body, the porous body having non-directional winding channels, the two sides of the porous body being respectively a first outer surface and a second outer surface, the porous body comprising a pre-filter layer and a separation layer, the separation layer being located on the side of the pre-filter layer close to the second outer surface, and the pre-filter layer and the separation layer being connected by a continuous fiber; the SEM-measured average pore diameter of the pre-filter layer is larger than that of the separation layer; the thickness of the separation layer is 5-60 mu m, and the SEM-measured average pore diameter of the separation layer is 25-85 nm; for PP7 phages, the top washing LRV of the virus-removal membrane is not less than 4, and the ratio of the top washing LRV of the virus-removal membrane to the initial LRV is not less than 0.7. The application further discloses a preparation process of the virus-removal membrane. The fiber virus-removal membrane has a high protein yield, and has a high virus-removal effect before and after top washing, so that the virus pollution risk can be reduced, and the economic benefit can be improved.

There is provided inexpensive means capable of exhibiting an inactivation effect on microorganisms without assuming the presence of light while minimizing the burden on the environment.Bacteria or viruses are inactivated by bringing a compound having a redox mechanism below into contact with a target suspected to be contaminated by bacteria or viruses:in the formula, X− represents a counteranion, and a broken line represents a bonding position with another atom.

A packaging system for a coronavirus pseudovirus, including a vesicular stomatitis virus (VSV) vector in which Fluc and EGFP dual-reporter genes replace a GP gene, and packaging cell that expresses a coronavirus spike protein. The packaging system may quickly package pseudoviruses by using a one-step packaging method, and may be used in the research of coronaviruses such as COVID-19 (SARS-CoV-2), SARS (SARS-CoV) and MERS, and other viruses. The packaging system and thereby pseudovirus method may also be used to evaluate the efficacy of disinfectants by means of virus contamination distribution models, setting up scenarios, and sampling and testing steps.

This invention discloses a method for preparing and applying cell vesicles derived from the rhizomes of traditional Chinese medicinal plants. The preparation method includes the following steps: washing and crushing the rhizomes of the medicinal plants, adding PBS buffer and grinding to obtain a homogenate; centrifuging the homogenate differentially and collecting the supernatant; subjecting the supernatant to a first ultracentrifugation to obtain a crude extract of cell vesicles; resuspending the crude extract of cell vesicles and loading it into a discontinuous sucrose density gradient solution for a second ultracentrifugation; collecting gradient bands with buoyant densities between 1.13 g / mL and 1.19 g / mL. This invention provides a stable and scalable preparation process that, through differential centrifugation combined with sucrose density gradient ultracentrifugation, can efficiently separate high-purity, structurally intact cell vesicles from the rhizomes of traditional Chinese medicinal plants such as Bletilla striata, Polygonatum sibiricum, and Curculigo orchioides. The process has good reproducibility and avoids the ethical and viral contamination risks associated with mammalian-derived vesicles.

Provided herein is a method of treating a material contaminated with a virus, the method comprising exposing the material to a vapor, wherein the vapor is produced by heating and / or vaporizing a dry cleaning solvent, and wherein the method at least partially inactivates the virus.