9results about How to "Maintain activity" patented technology

The application discloses a method for inducing T cell reprogramming into NK-like cells, comprising the following steps: (1) culturing the T cells in a culture system to obtain activated T cells; (2) adding small molecule inducers twice to the activated T cells to obtain NK-like cells; wherein the culture system in step (1) contains 1-10 v / v % serum substitute and / or platelet lysate, and does not contain fetal bovine serum. By optimizing the culture process and precisely adding drugs twice, the small molecule accumulation toxicity caused by multiple drug additions is avoided, the damage to the structure and function of the mitochondrial membrane is reduced, the mitochondrial membrane potential is significantly improved, the energy metabolism capacity of the mitochondria is enhanced, sufficient energy support is provided for the long-term survival and function maintenance of the cITNK cells, and the physiological activity of the cells is ensured from the energy metabolism level.

The application relates to the field of food biotechnology, and discloses a low-salt bean paste production process based on segmented temperature control fermentation, which is made of fava bean koji, water, edible salt, sodium hexametaphosphate, lactococcus lactis nisin, edible alcohol, granulated coarse granular calcium carbonate and glucono-delta-lactone. The production process comprises the following steps: constructing a composite matrix, and first performing buffer-type medium-temperature enzymolysis; then adding a glucono-delta-lactone aqueous solution in a flow manner, utilizing the stoichiometric coupling reaction of the glucono-delta-lactone aqueous solution and the granulated coarse granular calcium carbonate to generate carbon dioxide in situ to construct a micropore structure and regulate the pH value of the system; and finally, connecting a Zygosaccharomyces rouxii to perform low-temperature aroma-producing after-ripening. Through the synergistic effect of the components, the application effectively solves technical problems in the low-salt fermentation process, such as low protein solubility, easy compaction of the matrix and breeding of miscellaneous bacteria, and improves the amino acid nitrogen content and protein hydrolysis rate of the finished product.

The application discloses application of a microbial composition in industrial hazardous waste treatment and belongs to the technical field of industrial hazardous waste treatment and comprises the following steps: S1, collecting industrial hazardous waste, obtaining hazardous waste materials after pretreatment of the industrial hazardous waste, and filling the hazardous waste materials in a fermentation tank; S2, adding a growth medium in a bioreactor, connecting domesticated bacterial liquid to the bioreactor for expansion culture, and obtaining fermentation materials of compound acidophilic bacteria; S3, adding a biosurfactant to the fermentation materials of the compound acidophilic bacteria, uniformly stirring, and obtaining cell membrane surface hydrophobic modified compound acidophilic bacteria; and S4, then adding an acidic pH buffer solution to obtain a to-be-treated material. The application can maintain the stability of the pH required by the acidophilic bacteria in the process of treating the industrial hazardous waste and improve the cell membrane surface hydrophobicity of the compound acidophilic bacteria.

The application provides a method for detecting biochemical oxygen demand in sewage, and belongs to the field of oxygen demand detection. The method comprises the following steps: forming a fluorine-containing organosilicon nano coating on the electrode surface of a dissolved oxygen sensor of a full-automatic BOD5 analyzer to obtain a fluorine-silicon modified dissolved oxygen sensor; pretreating the sewage to be detected in an adsorption column composed of modified activated carbon and porous silica gel to obtain a pretreated water sample; fixing bacillus subtilis on a porous carrier and then putting the carrier into the pretreated water sample to obtain an inoculated water sample; measuring the initial dissolved oxygen of the inoculated water sample and the end-point dissolved oxygen after 5-day culture; and obtaining the five-day biochemical oxygen demand in the sewage according to the initial dissolved oxygen and the end-point dissolved oxygen. Through the four-level collaborative system of "sensor anti-interference optimization, sample purification and enrichment, microbial reaction standardization and accurate signal acquisition", the application systematically eliminates the error sources in the traditional BOD5 detection, and realizes the accurate measurement of the organic matter pollution level in complex sewage.

The present application relates to the technical field of water body bacteria inactivation, and particularly relates to a bacteria inactivation method based on micro-nano bubble ozone synergistic reaction, comprising the following steps: mixing and conveying ozone gas dissolved water and initial sewage to a front reactor to output as dissolved air sewage; conveying the dissolved air sewage to a reaction tank; obtaining the pressure of a plurality of pressure sampling positions of a conveying pipeline conveying the dissolved air sewage; determining the merging state of micro-nano bubbles in the conveying pipeline; determining an abnormal area in the merging state of the micro-nano bubbles; determining the bubble merging amount of the abnormal area based on the obtained conveying pipeline pressure of the position where the connecting port is located and the average pressure in the conveying pipeline behind the connecting port; determining the dosing amount of alkaline substances and the replenishment amount of the dissolved air water; and continuously conveying the dissolved air sewage from the front reactor to the reaction tank to complete the bacteria inactivation operation, so that the cavitation effect of ozone micro-nano bubbles is strengthened and the solubility of ozone in water is improved.

The invention discloses a preparation method of an anti-aging and life-prolonging cell nutrient rich in natural antioxidants for pets, and belongs to the field of nutritional supplements for pets. The preparation method comprises the following steps: firstly, mixing blueberry powder, broccoli powder, carrot powder, spinach powder, pomegranate extract, green tea extract, curcumin, fish oil, vitamin E, vitamin C and water according to a specific proportion; then, carrying out composite enzymolysis treatment, and fully releasing active substances in plant cells by using a synergistic effect of cellulase and pectinase; then, the enzymatic hydrolysate is fermented by lactic acid bacteria, nutritional ingredients are converted, and flavor substances are generated; then, lecithin is added for high-speed shearing, homogenizing and emulsifying, and stable emulsion is formed to protect fat-soluble components and improve physical properties; and finally, preparing a final product through an optimized spray drying process. According to the method, through scientific compatibility of raw materials and targeted process optimization, the problems of insufficient release of active ingredients, poor stability, low bioavailability, poor palatability and the like in the prior art are solved.

The application belongs to the technical field of microorganisms, and discloses a method for inducing formation of living unculturable state bacteria in the presence of host cells and application thereof. The method is as follows: cells in the 3th to 20th generation are transferred to a well plate to obtain induced cells; bacteria in the logarithmic growth phase are washed by a buffer, and modified cell culture solution is resuspended to obtain a bacterial suspension. The bacterial suspension is added to the induced cells, and after incubation in a constant-temperature incubator for 1-5 hours, 10-500 mg / L gentamicin-containing modified cell culture solution is added. After cell treatment for 3-10 days, intracellular bacteria are collected by adding cell lysis solution, and then washed, centrifuged and resuspended to obtain living unculturable state bacteria. The application can induce bacteria to enter the living unculturable state in the presence of host cells, which not only expands the understanding of living unculturable state bacteria, but also provides theoretical and technical support for recurrent and chronic infectious diseases in clinical practice.

This invention discloses a composite culture medium with defined components for co-culturing multiple cell types, along with its usage and applications. The culture medium can be used for long-term, stable triadic co-culture of patient-derived glioma stem cells, patient-derived T cells, and THP-1 macrophages (or patient-derived macrophages). The culture medium includes basal medium and EGF, bFGF, IL-2, M-CSF, ITS, and hydrocortisone. Furthermore, this invention provides optimized cell ratios and culture methods, achieving proliferation results that simultaneously maintain GSC stemness, primary T cell activity, and THP-1-derived macrophage activity for up to 14 days in the same system.

This invention discloses the construction and application of a xanthan gum cell factory with short fermentation cycle, high potency, and high resistance to oxidative stress. Using *Xanthomonas aeruginosa* XC1 as the starting strain, this invention employs a multi-step chromosome integration strategy to achieve the synergistic effect of three major modules: "enhancing quorum sensing signals, strengthening the rate-limiting synthesis step, and improving antioxidant performance." The resulting engineered strain EG-Y6 showed a 66-fold increase in intracellular DSF levels compared to the original strain XC1, significantly advancing the efficient xanthan gum synthesis period and shortening the fermentation cycle from 72 hours to 48 hours. The xanthan gum fermentation potency reached 26.9 g / L, a significant increase of 33.2% compared to the basic strain XC1. In the later stages of fermentation, the intracellular glutathione (GSH) content was 2.22 times higher than that of the basic strain XC1, while the reactive oxygen species (ROS) level decreased by 57%.