30results about How to "Maintain activity" patented technology

The invention discloses organic material and microbial agent coupled fertilization equipment and method, the organic material and microbial agent coupled fertilization equipment comprises a mixing box, the bottom of the mixing box is of a semicircular structure, and the upper part of the mixing box is of a rectangular structure; a partition plate is fixedly connected to the upper portion of the mixing box and divides the upper space of the mixing box into an organic material feeding cavity and a soil feeding cavity. A storage box is arranged on one side, close to the soil feeding cavity, of the upper part of the mixing box; a circular cavity is formed in the bottom of the mixing box; a mixing barrel with two closed ends is rotationally connected into the circular cavity; firstly, a microbial agent and a carrier are preliminarily mixed, then the coupled microbial agent is put into a mixing barrel, the clay carrier and the microbial agent are mixed to protect the microbial agent, the microbial agent is not directly subjected to violent reaction or excessive stirring with other materials, the shearing force of a stirring rod is reduced, and the use of the soil carrier can help maintain the stability and activity of the microbial agent; meanwhile, the uniform distribution of the fungicide and the organic materials is ensured.

The invention belongs to the technical field of biology, and particularly relates to a genetically modified mesenchymal stem cell as well as a preparation method and application thereof. The invention relates to a gene modified mesenchymal stem cell, the gene modified mesenchymal stem cell is a mesenchymal stem cell of an over-expression SIRT6 gene, and the acquisition number of the SIRT6 on NCBI (National Center of Biotechnology Information) is NM001193285. The invention finds that SIRT6 is helpful for delaying the cell senescence process and obviously improving the anti-senescence capability of cells by regulating senescence-related genes and proteins.

The invention relates to the field of acupuncture and cupping disinfection, in particular to a quick disinfection device for acupuncture and cupping, which comprises a disinfection table, a processing unit is arranged on the disinfection table, and the processing unit comprises a disinfection cylinder fixed on the disinfection table, a cleaning mechanism for cleaning a cupping device and a clamping mechanism for fixing the cupping device. The cleaning mechanism comprises an inner brush part and an outer brush part, a driving shaft used for driving the inner brush part to rotate is coaxially arranged in the disinfection cylinder, and a connecting structure used for ensuring that the cupping device is clamped between the inner brush part and the outer brush part is arranged between the inner brush part and the outer brush part. The inner brush part and the outer brush part are tightly attached to the cupping device, the cleaning comprehensiveness is ensured, then the cupping device is locked by the clamping mechanism, the inner brush part and the outer brush part cooperatively work, the inner surface and the outer surface of the cupping device are deeply cleaned while the cupping device is kept relatively static, and the disinfection comprehensiveness and efficiency are improved.

A kind of vocal cord repair device based on advanced biological printing technology assisted, comprising: imaging positioning component, including endoscope imaging module and three-dimensional reconstruction processor;Surgical operating component, including glottis exposure mechanism and elephant trunk type flexible mechanical arm, glottis exposure mechanism exposes glottis to provide field of view, and mechanical arm high-precision operation;Biological repair component, including micro printing head, compatible hydrogel, injection tube and motion control mechanism, realize vocal cord reconstruction and function recovery.Integrate biological printing component and imaging positioning component, system can accurately obtain the three-dimensional structure data of vocal cord damage area, and realize the millimeter level accurate deposition of biological material, so that the millimeter level precision of imaging positioning, micron level operation of mechanical arm and controllable printing of biological material form closed loop feedback system, improve the overall success rate of vocal cord repair, realize the leap of vocal cord repair from "empirical operation" to "precision engineering", provide safer and more effective treatment options for patients with vocal cord dysfunction.

The invention discloses a preparation method of a high-specific-surface-area polymer-based magnesium artificial wetland filter material. The preparation method comprises the following steps: firstly, respectively preparing a water phase containing magnesium salt and an emulsifier, and an organic phase; then slowly dropwise adding the water phase into the organic phase in a trickling filtration manner, so that the water phase forms tiny liquid drops in the organic phase, a polymerization reaction is carried out on an interface, and a magnesium ion embedded polymer matrix is formed; carrying out foaming treatment on the polymer matrix embedded with the magnesium ions, and rapidly cooling; and finally, separating a blank body through suction filtration, cleaning, drying and screening to obtain the filter material. The filter material obtained by the method disclosed by the invention can regulate the pH value of a water body and slowly release magnesium ions, enhance the nutrient supply of plants and improve the wetland function in a low-temperature environment, and has a high specific surface area.

The application discloses a method for inducing T cell reprogramming into NK-like cells, comprising the following steps: (1) culturing the T cells in a culture system to obtain activated T cells; (2) adding small molecule inducers twice to the activated T cells to obtain NK-like cells; wherein the culture system in step (1) contains 1-10 v / v % serum substitute and / or platelet lysate, and does not contain fetal bovine serum. By optimizing the culture process and precisely adding drugs twice, the small molecule accumulation toxicity caused by multiple drug additions is avoided, the damage to the structure and function of the mitochondrial membrane is reduced, the mitochondrial membrane potential is significantly improved, the energy metabolism capacity of the mitochondria is enhanced, sufficient energy support is provided for the long-term survival and function maintenance of the cITNK cells, and the physiological activity of the cells is ensured from the energy metabolism level.

The application relates to the field of food biotechnology, and discloses a low-salt bean paste production process based on segmented temperature control fermentation, which is made of fava bean koji, water, edible salt, sodium hexametaphosphate, lactococcus lactis nisin, edible alcohol, granulated coarse granular calcium carbonate and glucono-delta-lactone. The production process comprises the following steps: constructing a composite matrix, and first performing buffer-type medium-temperature enzymolysis; then adding a glucono-delta-lactone aqueous solution in a flow manner, utilizing the stoichiometric coupling reaction of the glucono-delta-lactone aqueous solution and the granulated coarse granular calcium carbonate to generate carbon dioxide in situ to construct a micropore structure and regulate the pH value of the system; and finally, connecting a Zygosaccharomyces rouxii to perform low-temperature aroma-producing after-ripening. Through the synergistic effect of the components, the application effectively solves technical problems in the low-salt fermentation process, such as low protein solubility, easy compaction of the matrix and breeding of miscellaneous bacteria, and improves the amino acid nitrogen content and protein hydrolysis rate of the finished product.

An anti-MASP2 antibody, an antigen-binding fragment thereof and the medical use thereof. Specifically, provided are an anti-MASP2 antibody or an antigen-binding fragment thereof, a pharmaceutical composition thereof, a method for treating diseases such as IgA nephropathy and a related pharmaceutical use.

The application discloses application of a microbial composition in industrial hazardous waste treatment and belongs to the technical field of industrial hazardous waste treatment and comprises the following steps: S1, collecting industrial hazardous waste, obtaining hazardous waste materials after pretreatment of the industrial hazardous waste, and filling the hazardous waste materials in a fermentation tank; S2, adding a growth medium in a bioreactor, connecting domesticated bacterial liquid to the bioreactor for expansion culture, and obtaining fermentation materials of compound acidophilic bacteria; S3, adding a biosurfactant to the fermentation materials of the compound acidophilic bacteria, uniformly stirring, and obtaining cell membrane surface hydrophobic modified compound acidophilic bacteria; and S4, then adding an acidic pH buffer solution to obtain a to-be-treated material. The application can maintain the stability of the pH required by the acidophilic bacteria in the process of treating the industrial hazardous waste and improve the cell membrane surface hydrophobicity of the compound acidophilic bacteria.

The invention belongs to the field of tablet candy preparation, and particularly relates to an edible mushroom extract-containing tablet candy and a preparation method thereof. The preparation method comprises the following steps: mixing polyvinyl alcohol and distarch phosphate to form distarch phosphate gel, performing frozen nano spray drying, and fully adsorbing the caries-preventing mixture, so that the prepared caries-preventing and blood sugar-reducing colloid particles have the effects of preventing caries and reducing blood sugar; a porous enzymolysis product obtained through enzymolysis is mixed with polyglycerol fatty acid ester and a plant extracting solution, and the prepared composition capable of accelerating disintegration and maintaining activity can rapidly absorb saliva and expand and disintegrate; the surface of the candy tablet is sprayed with an anti-oxidation anti-cracking solution, the tabletted candy containing the edible mushroom extract after film formation does not cause caries after being eaten for a long time, the disintegration speed is not affected after long-time storage, and tablet cracking is avoided.

The application provides a method for detecting biochemical oxygen demand in sewage, and belongs to the field of oxygen demand detection. The method comprises the following steps: forming a fluorine-containing organosilicon nano coating on the electrode surface of a dissolved oxygen sensor of a full-automatic BOD5 analyzer to obtain a fluorine-silicon modified dissolved oxygen sensor; pretreating the sewage to be detected in an adsorption column composed of modified activated carbon and porous silica gel to obtain a pretreated water sample; fixing bacillus subtilis on a porous carrier and then putting the carrier into the pretreated water sample to obtain an inoculated water sample; measuring the initial dissolved oxygen of the inoculated water sample and the end-point dissolved oxygen after 5-day culture; and obtaining the five-day biochemical oxygen demand in the sewage according to the initial dissolved oxygen and the end-point dissolved oxygen. Through the four-level collaborative system of "sensor anti-interference optimization, sample purification and enrichment, microbial reaction standardization and accurate signal acquisition", the application systematically eliminates the error sources in the traditional BOD5 detection, and realizes the accurate measurement of the organic matter pollution level in complex sewage.

The present application relates to the technical field of water body bacteria inactivation, and particularly relates to a bacteria inactivation method based on micro-nano bubble ozone synergistic reaction, comprising the following steps: mixing and conveying ozone gas dissolved water and initial sewage to a front reactor to output as dissolved air sewage; conveying the dissolved air sewage to a reaction tank; obtaining the pressure of a plurality of pressure sampling positions of a conveying pipeline conveying the dissolved air sewage; determining the merging state of micro-nano bubbles in the conveying pipeline; determining an abnormal area in the merging state of the micro-nano bubbles; determining the bubble merging amount of the abnormal area based on the obtained conveying pipeline pressure of the position where the connecting port is located and the average pressure in the conveying pipeline behind the connecting port; determining the dosing amount of alkaline substances and the replenishment amount of the dissolved air water; and continuously conveying the dissolved air sewage from the front reactor to the reaction tank to complete the bacteria inactivation operation, so that the cavitation effect of ozone micro-nano bubbles is strengthened and the solubility of ozone in water is improved.

This invention relates to the field of biomedical engineering technology, specifically to a biopolymer film, its preparation method, and its applications. The biopolymer film is mainly composed of the following components: gelatin, sodium alginate, purple cabbage anthocyanin extract, and tannic acid-modified zinc oxide nanoparticles. The preparation method is as follows: tannic acid-modified zinc oxide nanoparticles are dispersed in an acidified solution, gelatin and sodium alginate are added, and the mixture is stirred to dissolve, obtaining a first mixed solution; purple cabbage anthocyanin extract is dissolved in water, tannic acid is added, and the mixture is stirred to dissolve, obtaining a second mixed solution; the first and second mixed solutions are mixed evenly to obtain a film-forming solution; the film-forming solution is poured into a mold and dried to form a film, thus obtaining the biopolymer film. The biopolymer film of this invention integrates the cross-linking enhancement, anthocyanin stabilization, and antibacterial functions of tannic acid, solving the problems of existing biopolymer films requiring multiple additives, complex processes, and functional constraints.

The invention discloses a preparation method of an anti-aging and life-prolonging cell nutrient rich in natural antioxidants for pets, and belongs to the field of nutritional supplements for pets. The preparation method comprises the following steps: firstly, mixing blueberry powder, broccoli powder, carrot powder, spinach powder, pomegranate extract, green tea extract, curcumin, fish oil, vitamin E, vitamin C and water according to a specific proportion; then, carrying out composite enzymolysis treatment, and fully releasing active substances in plant cells by using a synergistic effect of cellulase and pectinase; then, the enzymatic hydrolysate is fermented by lactic acid bacteria, nutritional ingredients are converted, and flavor substances are generated; then, lecithin is added for high-speed shearing, homogenizing and emulsifying, and stable emulsion is formed to protect fat-soluble components and improve physical properties; and finally, preparing a final product through an optimized spray drying process. According to the method, through scientific compatibility of raw materials and targeted process optimization, the problems of insufficient release of active ingredients, poor stability, low bioavailability, poor palatability and the like in the prior art are solved.

The present invention relates to a hybrid catalyst composition comprising a heterogeneous transition metal compound, a catalyst for olefin polymerization comprising same, and a method for preparing an olefin-based polymer in which generation of a gel is suppressed using the catalyst. The method for preparing an olefin-based polymer according to an embodiment of the present invention can provide an olefin-based polymer in which non-uniformity of the olefin-based polymer, and in particular, generation of a gel is suppressed.

The application belongs to the technical field of microorganisms, and discloses a method for inducing formation of living unculturable state bacteria in the presence of host cells and application thereof. The method is as follows: cells in the 3th to 20th generation are transferred to a well plate to obtain induced cells; bacteria in the logarithmic growth phase are washed by a buffer, and modified cell culture solution is resuspended to obtain a bacterial suspension. The bacterial suspension is added to the induced cells, and after incubation in a constant-temperature incubator for 1-5 hours, 10-500 mg / L gentamicin-containing modified cell culture solution is added. After cell treatment for 3-10 days, intracellular bacteria are collected by adding cell lysis solution, and then washed, centrifuged and resuspended to obtain living unculturable state bacteria. The application can induce bacteria to enter the living unculturable state in the presence of host cells, which not only expands the understanding of living unculturable state bacteria, but also provides theoretical and technical support for recurrent and chronic infectious diseases in clinical practice.

Provided are an anti-CD40 antibody, an antigen-binding fragment and a medical use thereof, as well as a pharmaceutical composition containing the anti-CD40 antibody or antigen-binding fragment thereof, and a method for treating and preventing disease, in particular a method for treating autoimmune disease.

The invention belongs to the technical field of mechanical structures, and particularly relates to a grain tank device of a combine harvester, which comprises a tank body, a grain outlet cylinder, a gantry guide mechanism and a driving mechanism. The portal frame guide mechanism comprises a portal frame base, a left portal frame, a right portal frame and a grain tank support. The left side door frame and the right side door frame are arranged in parallel and are vertically and fixedly connected with the door frame base respectively; the two ends of the grain tank support are slidably connected with the left door frame and the right door frame respectively, and the tank body is installed on the grain tank support. The driving mechanism is installed on the portal guide mechanism and cooperates with the portal guide mechanism to control the box body to ascend or descend. One end of the grain outlet cylinder is rotationally communicated and connected with one side of the bottom of the box body; the flashboard assembly is arranged at the other end; a rotary drum handle is arranged on the side face of the grain discharging drum. According to the technical scheme, the grain breakage rate is reduced by adopting a brand-new structural design; the grain discharging barrel can flexibly rotate to realize accurate grain unloading; the unloading process is optimized by the box body structure and the driving mechanism; the design of the flashboard assembly, the pulley and other assemblies provides convenient control experience.

The invention belongs to the technical field of planting, and discloses a method for promoting the growth of an atractylodes lancea plant by inoculating arbuscular mycorrhizal fungi, and the inoculating method comprises the following steps: step 1, obtaining the arbuscular mycorrhizal fungi, step 2, preparing a sterilization matrix, step 3, carrying out mixed planting, step 4, preparing a microbial inoculum, and step 5, inoculating the microbial inoculum. According to the method provided by the invention, the mycorrhiza infection rate of the rhizoma atractylodis chinensis root system can reach 100%, and the biomass of the rhizoma atractylodis chinensis is remarkably improved; the dependence of the current year and annual atractylodes lancea on mycorrhiza reaches 36% and 52% respectively, and the dry weight of the whole plant is increased by 56.2% and 108.4% respectively; meanwhile, the root length and the root surface area of the next-year atractylodes lancea inoculated with the inoculant are obviously higher than those of the non-inoculated atractylodes lancea; mycorrhizal seedlings formed by the method have good growth vigor and well-developed root systems, and the adaptability and the survival rate of rhizoma atractylodis chinensis are improved.

The present invention provides a cryopreservation solution, a preparation method and a use method for kidney organoids, and relates to the field of cell and tissue engineering technology. The cryopreservation solution includes the following components by weight: 4.7 to 5.3 parts of cell cryopreservation solution; 3.5 to 4.5 parts of KOSR serum substitute; 0.8 to 1.2 parts of improved culture medium; wherein the improved culture medium includes RPMI 1640 culture medium and L-alanyl-L-glutamine, and the weight of RPMI 1640 culture medium is 3.5 to 4.5 times that of L-alanyl-L-glutamine. KOSR serum substitute and improved culture medium are added to CS10 cell cryopreservation solution to dilute the concentration of dimethyl sulfoxide, so that the dimethyl sulfoxide in the cryopreservation solution reaches the required minimum concentration, which not only reduces the formation of ice crystals, but also avoids the cytotoxicity caused by the excessively high concentration of dimethyl sulfoxide, thereby reducing the damage of kidney organoids.

The utility model discloses a device for treating papermaking fiber wastewater, which is applied to the field of papermaking wastewater treatment and comprises a biochemical reaction shell, a biological treatment mechanism is arranged in the biochemical reaction shell, and a pretreatment mechanism is arranged on one side of the biochemical reaction shell. When the papermaking wastewater is treated in the biological treatment mechanism, solid wastes such as particulate matters, precipitates and suspended solids in the papermaking wastewater can be treated in advance through the pretreatment mechanism, so that the load of subsequent biochemical treatment is reduced, the treatment effect is improved, and the solid wastes can be recycled before the papermaking wastewater is subjected to biological treatment. Solid wastes such as particulate matters, sediments and suspended matters in the papermaking process can be removed, the load of a subsequent biochemical treatment device is favorably reduced, and the stability and the treatment effect of the whole system are improved.

The invention provides a two-stage coal-to-ethylene glycol hydrogenation reactor and a use method thereof, and relates to the technical field of chemical production. A traditional tubular reactor has the problems of short service life caused by coking of an upper-layer catalyst, activity waste of a lower-layer catalyst and the like. According to the scheme, a two-section tubular reactor is adopted, the material is 304, the inner diameter phi is 38, the upper part of the reactor is a first reactor section, the lower part of the reactor is a second reactor section, and a mixed gas distributor is arranged between the first reactor section and the second reactor section. And the reactor first section and the reactor second section are designed to be two-section type cooling water feeding design of cooling water feeding and cooling water returning. The upper and lower two-section feeding and two-section cooling design can adjust the feeding amount according to working conditions, slow down coking at the upper part of a bed layer, prolong the service life of a catalyst, fully utilize the activity of the catalyst at the lower section, improve the heat exchange effect, guarantee safe operation, effectively optimize the reaction process and conditions, and improve the efficiency and economical efficiency of the coal-to-ethylene glycol hydrogenation process.

The invention discloses novel bacterial agent dry powder capable of efficiently maintaining strain activity and a preparation method of the novel bacterial agent dry powder. The preparation method comprises the following specific steps: (1) selecting five dominant strains, namely A.faecalis S1, Bacillus megaterium XN1, Bacillus subtilis XN2, Raoultella sp.XY-1 and Pandoraea sp.XY-2, which are capable of efficiently degrading tetracycline, and carrying out ultrasonic treatment on the dominant strains, so as to obtain a strain A for efficiently degrading tetracycline; (2) performing amplification culture on the strain obtained in the step (1), and then preparing a composite flora according to the ratio of 1: 1: 2: 3: 2; (3) mixing the composite flora obtained in the step (2) with a phyllostachys pubescens substrate according to the ratio of 1: 5, 1: 10, 1: 15 and 1: 20 to obtain different microbial agents, detecting the effects of the microbial agents respectively, and determining the optimal ratio; and (4) adding 10% of a trehalose solution into the microbial agent with the optimal proportion obtained in the step (3), mixing, carrying out low-temperature vacuum filtration, and carrying out vacuum freeze drying, so as to prepare the microbial agent dry powder. The microbial inoculum dry powder prepared by the invention can effectively improve the activity and stability of the strain. In addition, the removal rate of tetracycline in tetracycline (60 mg / L) type wastewater by the dry powder of the microbial agent is 89.31%, and the microbial agent has a good application prospect in environmental treatment of tetracycline type wastewater.

This invention discloses a composite culture medium with defined components for co-culturing multiple cell types, along with its usage and applications. The culture medium can be used for long-term, stable triadic co-culture of patient-derived glioma stem cells, patient-derived T cells, and THP-1 macrophages (or patient-derived macrophages). The culture medium includes basal medium and EGF, bFGF, IL-2, M-CSF, ITS, and hydrocortisone. Furthermore, this invention provides optimized cell ratios and culture methods, achieving proliferation results that simultaneously maintain GSC stemness, primary T cell activity, and THP-1-derived macrophage activity for up to 14 days in the same system.

This invention discloses the construction and application of a xanthan gum cell factory with short fermentation cycle, high potency, and high resistance to oxidative stress. Using *Xanthomonas aeruginosa* XC1 as the starting strain, this invention employs a multi-step chromosome integration strategy to achieve the synergistic effect of three major modules: "enhancing quorum sensing signals, strengthening the rate-limiting synthesis step, and improving antioxidant performance." The resulting engineered strain EG-Y6 showed a 66-fold increase in intracellular DSF levels compared to the original strain XC1, significantly advancing the efficient xanthan gum synthesis period and shortening the fermentation cycle from 72 hours to 48 hours. The xanthan gum fermentation potency reached 26.9 g / L, a significant increase of 33.2% compared to the basic strain XC1. In the later stages of fermentation, the intracellular glutathione (GSH) content was 2.22 times higher than that of the basic strain XC1, while the reactive oxygen species (ROS) level decreased by 57%.

The present invention belongs to the technical field of flue gas denitration, discloses a preparation method of a CO-SCR denitration catalyst, a CO-SCR denitration catalyst and a flue gas denitration method, and solves the technical problem that there is no high-performance CO-SCR catalyst with high strength, low cost and the ability to overcome oxygen-rich conditions in the prior art. The preparation method of the CO-SCR denitration catalyst comprises the following steps: mixing a primary carbonized material and a first adhesive solution by ball milling to obtain a mixture; mixing the mixture, a reinforced resin powder and a second adhesive solution by ball milling, and then extruding to obtain a pressed blank; activating the pressed blank to obtain activated carbon; immersing the activated carbon in a first modified solution, filtering, drying and roasting after the immersion is completed to obtain a modified activated carbon; the first modified solution contains a soluble transition metal salt; immersing the modified activated carbon in a second modified solution, filtering and drying after the immersion is completed to obtain a CO-SCR denitration catalyst.

The present invention provides a whitening, moisturizing skin care composition and preparation method thereof, including the following raw materials: plant extract, modified ceramide, nicotinamide, magnesium ascorbyl phosphate, panthenol, lactobionic acid, dipalmitoyl hydroxyproline, dipotassium glycyrrhizinate, arbutin, phenylethyl resorcinol, 4-butyl resorcinol, xanthan gum, hydroxypropyl cyclodextrin, methyl propanediol, and the balance is water; the plant extract includes one or more combinations of licorice root extract, selaginella extract, cactus extract, scutellaria root extract, peony root extract, and Lysimachia extract. The raw materials of the present invention are scientifically and rationally compounded, can effectively promote the whitening and moisturizing effect of active ingredients, reduce dullness, spots and dehydration of skin, can also resist the damage of external environment to skin, can also soften the stratum corneum of the outer layer of skin, clean the outer layer of skin, promote skin metabolism, reduce aging, and maintain the elasticity and vitality of skin.

The present invention discloses a method for synthesizing diphenylamine from aniline. The method of the present invention comprises the following contents: (1) providing a reactor, wherein the reactor comprises at least one catalyst bed; (2) introducing aniline into the reactor, passing through the catalyst bed under reaction conditions, and subjecting the reaction effluent to gas-liquid separation and fractionation to obtain a diphenylamine product; wherein, during the reaction, an amine-soluble stabilizer is introduced into the reactor; the amine-soluble stabilizer has a boiling point higher than that of aniline and is stable at high temperatures. By adding the amine-soluble stabilizer, the present invention allows the reaction to be carried out at high temperatures, reduces the large amount of by-products produced and the rapid deactivation of the catalyst, and not only improves the selectivity of diphenylamine, but also prolongs the life of the catalyst.

The invention relates to the technical field of cell culture, and particularly discloses a myocardial cell culture method which comprises the following steps: 1) selecting an experimental subject capable of carrying out myocardial cell culture; 2) separating the cardiac muscle tissue of the experimental subject; 3) separating cardiac muscle cells from the separated cardiac muscle tissue; and (4) adding the separated myocardial cells into a high-glucose DMEM culture solution, then adding 5-bromodeoxyuridine, uniformly mixing, inoculating into a culture dish which is coated with polyglutamic acid overnight at 37 DEG C in advance according to the density of (1.0-6.0) * 10 < 5 > cells / cm < 2 >, and culturing in a 5% CO2 cell culture box at 37 DEG C, after 48h, replacing a high-glucose DMEM culture solution which does not contain 5-bromodeoxyuridine and contains 15% of fetal calf serum, and subsequently replacing the high-glucose DMEM culture solution which does not contain 5-bromodeoxyuridine and contains 15% of fetal calf serum according to the growth state of the cells for 1d-2d for continuous culture. The myocardial cell culture method provided by the invention can efficiently and stably obtain a large number of myocardial cells with good activity, high survival rate and high purity.