78 results about "Low protein" patented technology

The invention relates to the technical field of biological separation materials, and discloses an amine-functionalized magnetic nano composite material and application thereof in protein separation and purification. The material has a core-shell structure and comprises a magnetic ferroferric oxide nano core, an ordered mesoporous silicon dioxide intermediate layer and a covalent grafted polyethyleneimine functional layer. By controlling the matching relationship between the pore diameter of mesopores and the molecular size of the polymer and adopting a crosslinking strategy, the material has high magnetic responsiveness, rapid mass transfer performance and high-density surface amino groups. The material shows high adsorption capacity, rapid adsorption kinetics and good cycle stability in the protein separation process, and is suitable for enrichment purification and metal chelating affinity separation of protein in a biological sample. According to the invention, the technical problems of low protein adsorption capacity, slow dynamics and poor cycling stability caused by compact shell layer, non-uniform amino distribution and limited mass transfer of the existing amine functionalized magnetic material are solved.

The present invention relates to a Single Nucleotide Polymorphism (SNP) marker for determining the degree of protein content of rice seeds and a method for determining the degree of protein content of rice seeds using said marker. Specifically, the present invention relates to an SNP marker for determining protein content and a method for determining protein content using said marker, which constructs a machine learning-based rice protein content prediction model by utilizing SNP markers associated with high and low protein content, thereby enabling the prediction of rice seeds with high protein content in the early stages of growth.

This invention pertains to the purification of microbial fermentation broth, specifically a method for purifying fermentation broth containing β-1,3 / α-1,3-glucan. The method involves ethanol extraction and filtration to extract β-1,3 / α-1,3-glucan, including fermentation broth pretreatment, pH adjustment of the clarified broth after filtration, at least two ethanol extractions and filtrations, and drying and pulverizing the resulting fibrous material to obtain β-1,3 / α-1,3-glucan. This invention solves the problems of low extraction rate and low product purity in existing technologies, offering advantages such as high extraction rate and product yield, low protein and impurity content, and low production cost.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.

The invention belongs to the technical field of food processing, and discloses a low-GI protein bar and a preparation method thereof. The low-GI protein bar comprises the following raw materials in parts by mass: 32-48 parts of pretreated egg white liquid, 16-24 parts of whole wheat flour, 8-12 parts of oatmeal, 5.5-8.5 parts of bamboo shoot dietary fibers, 1.5-2.5 parts of chia seeds, 7-11 parts of date palm paste, 4.5-7.5 parts of a sweetening agent and 11-17 parts of auxiliary materials, wherein a preparation method of the pretreated egg white liquid comprises the following steps: adding an acid solution into the egg white liquid to adjust the pH value to 6.5-7, and then carrying out protease enzymolysis treatment. The method effectively solves the problems that a traditional protein bar with egg white as a core protein source is obvious in fishy smell, dry, hard and rough in texture, prone to cracking and scaling in appearance and the like, and the prepared low-GI protein bar is excellent in overall sensory quality and has the nutritional characteristics of being high in protein, high in fiber and low in GI.

This invention provides the application of substances targeting the CYTB-187AA gene and / or protein in the preparation of drugs for delaying aging and / or prolonging lifespan. The research results of this invention indicate that by reducing the expression of CYTB-187AA protein and / or inhibiting its activity, the aging process can be effectively delayed, thereby achieving the goal of extending healthy lifespan or lifespan. The research results of this invention are of great significance for scientific research on aging mechanisms and the development of anti-aging intervention strategies.

The invention aims to provide a method, equipment, medium and program product for determining binding protein information with pH sensitivity, and the method comprises the following steps: determining the binding protein information based on the target protein information, the target amino acid corresponding to the binding region amino acid of the target protein and the noise information of the binding protein in the initial time step; according to a first binding protein generation model, binding protein information corresponding to the target protein is generated, the target amino acid and binding region amino acid of the target protein are matched with preset pH sensitivity information, and binding protein sequence information in the binding protein information contains the target amino acid; the first binding protein generation model includes a first flow matching predictive model. A velocity field from noise data to binding protein information is predicted through a flow matching method, and the pH sensitivity of the binding protein is improved by introducing target amino acid in each step of prediction, so that ideal binding protein information is generated, the experience requirement of a protein designer is reduced, and the design efficiency is improved.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

The invention relates to a wet spinning preparation process of protein modified polyacrylonitrile fibers, and relates to the field of wet spinning preparation processes, the wet spinning preparation process comprises the following steps: S1, heating and softening a spinning stock solution, dropwise adding a protein solution, and fully dissolving to obtain a clear and transparent protein modified spinning solution; s2, the protein modified spinning solution is sequentially subjected to filtering, defoaming, spinning, coagulating bath and drafting, and then the protein modified polyacrylonitrile fiber is obtained; the spinning stock solution is an acrylonitrile-methyl acrylate-itaconic acid ternary copolymerized stock solution. The acrylonitrile-methyl acrylate-itaconic acid ternary copolymerized stock solution is adopted as a spinning matrix, the compatibility with natural protein is improved by introducing a polar group, and the protein is uniformly dispersed at a molecular level in cooperation with a mode of dropwise adding the protein solution after heating and softening, so that the protein agglomeration phenomenon is remarkably reduced, and the uniformity of the internal structure of the fiber is improved.

The application discloses a kind of double crosslinking food gels of functional factor and preparation method thereof, belong to food processing technical field.The application uses protein, edible oil, kappa-carrageenan, gellan gum and the like as main gel base material, first, potassium ion is used to induce the formation protein-kappa-carrageenan or protein-gellan gum composite gel, then composite gel is immersed in acidic solution and induced to form double crosslinking protein-kappa-carrageenan or protein-gellan gum composite gel.The method can be used for the preparation of protein-kappa-carrageenan or protein-gellan gum composite hydrogel and composite emulsion gel, and double crosslinking gel can be prepared at lower protein and polysaccharide concentration, which can significantly improve the hardness of gel, reduce the swelling rate of gel, and the double crosslinking composite gel prepared can realize the co-embedding of hydrophilic and hydrophobic functional factors, significantly improve the stability and controlled release characteristics of functional factors.

The invention relates to the technical field of livestock breeding and feed nutrition, and discloses a low-protein diversified daily ration design method and system based on multi-source data fusion, and the method comprises the steps: constructing a formula-environment-effect directed causal diagram through a PC algorithm, estimating the effect deviation contribution proportion of an environment factor and a formula factor through anti-factual reasoning, and calculating the effect deviation contribution proportion of the environment factor and the formula factor; and inferring the target weight preference of the farm by using inverse reinforcement learning, and carrying out conditional weight updating according to a causal attribution result. Real reasons of growth effect deviation can be distinguished, effect deviation errors caused by environmental factors are prevented from being attributed to improper formula setting, and the accuracy and stability of formula recommendation are improved.

The invention discloses a preparation method and application of a fat-soluble drug solid dispersion based on a zwitterionic hyperbranched polymer. According to the solid dispersion, a zwitterionic hyperbranched polymer HP-CB is used as a carrier, and a fat-soluble drug is loaded in an internal hydrophobic cavity; the preparation method of the solid dispersion comprises the following steps: loading a fat-soluble drug in a polymer by using a solvent evaporation method, freeze-drying to form the solid dispersion, and further compounding the solid dispersion with a cream matrix to prepare the nanoparticle composite cream. The solid dispersion has excellent hydrophilicity, low protein adsorptivity and good biocompatibility, the drug solubility and the transdermal permeability are remarkably improved, drug crystallization is effectively inhibited, the stability of a preparation is improved, and further prepared cream has the advantages of softening cuticles, promoting hydration and enhancing the sealing performance and has good application prospects. The traditional Chinese medicine composition can effectively improve chronic inflammatory skin diseases such as psoriasis, and has good clinical application prospect and industrialization value.

The present application relates to the technical field of meal replacement powder, and discloses a high-protein satiety meal replacement powder containing rice and pea protein and a preparation method thereof.The meal replacement powder comprises a protein component, dietary fiber, high-quality oil, a sweetener, trace nutrients, a stabilizer and a flavor component; wherein the protein component is a composite protein powder prepared by homogenizing, high-temperature shearing and spray drying of rice protein and pea protein.The present application utilizes the complementary characteristics of amino acids of rice protein and pea protein to achieve balanced nutrition, low allergenicity and easy digestion; the combination of dietary fiber, high-quality oil and composite trace nutrients takes into account high protein, high satiety and comprehensive nutrition; the product is prepared by a segmented mixing process and has good stability, diverse flavors and convenient preparation, and is suitable for people with lactose intolerance, weight control, exercise and other groups, solving the technical problems of low protein content, high allergenicity, nutritional imbalance and strong beany odor of traditional meal replacement powder, and being suitable for industrialized popularization and application.

The application relates to the glove production technical field, in particular to a condensing equipment for low-protein durable oxford glove production, which comprises a condensing box, a base, a cooling box and a cooling box. The upper end of the condensing box is a slide rail, the upper end of the slide rail is a conveying chain, the conveying chain is provided with a mounting plate, adjacent mounting plates arranged on the conveying chain are fixedly provided with connecting shafts, movable blocks are movably arranged on the connecting shafts, conveying assemblies are arranged at the lower ends of the movable blocks, and oxford gloves are arranged at the lower ends of the conveying assemblies. The condensing equipment for low-protein durable oxford glove production is provided with a nitrogen assembly and a refrigeration assembly which are combined to cyclically and reciprocally deliver the refrigerated air into the cooling box, then the reflux assembly arranged in the cooling box is started, the cooling liquid filled in the condensing box is subjected to heat exchange with the cold air introduced into the cooling box, and then is delivered into the condensing box for use again, so that the cooling liquid does not need to be replaced.

The invention discloses an integrated feeding control method for acetic acid group single-cell protein fermentation and application of the integrated feeding control method, and belongs to the technical field of microbial fermentation. By monitoring the change of the pH value of the fermentation liquid in real time, the composite material supplementing system containing the carbon and nitrogen sources is automatically triggered, pH signals are converted into the supplementing amount of the carbon sources and the nitrogen sources, the stable carbon-nitrogen ratio of supplemented materials in the fermentation process is maintained, and meanwhile the pH value is maintained in an appropriate range. The thallus yield and the protein content of the single-cell protein are obviously improved; the problems of poor thallus growth, low protein content and large substrate residual quantity caused by mutual independence of a carbon source, a nitrogen source and pH adjustment, tedious control of a fermentation process and imbalance of a carbon-nitrogen ratio in an existing material supplementing mode are solved.

The present application belongs to the technical field of feed additives, and particularly relates to a feed additive for improving ATP utilization rate of animal muscle cells, a preparation method and application. The feed additive comprises the following raw material components: guanidoacetic acid, methionine, betaine, vitamin E, vitamin B12, vitamin B6, folic acid, L-ascorbic acid, L-carnitine, selenium yeast and stearic acid. The present application supplements exogenous creatine synthesis raw materials for the animal body, improves the efficiency of conversion of guanidoacetic acid into creatine in the animal body, significantly increases the creatine level in the animal body, improves the ATP utilization efficiency of the animal body under the condition of lower nutrition level (low energy, low protein), reduces the conversion of nutrients such as protein into energy for consumption, and further significantly improves the growth performance and slaughter performance of the animal. The raw materials of the present application are all nutritional additives, and do not produce residues, are green and safe, meet the urgent needs of the rapid development of current antibiotic-free livestock farming, and have a wide application prospect.

The invention discloses an improved optimization process for improving yeast fermentation expression protein, which is characterized in that through dynamic temperature-pH coupling control and synergistic improvement of culture medium components, foreign protein expression quantity (gt, 90%) and biological activity (gt, 100%) are remarkably improved, and protein aggregate formation is reduced. The process has high magnification and is suitable for the field of biological pharmacy.

The invention discloses a vegetable protein fermentation production process, belongs to the field of vegetable protein deep processing, and aims to solve the problems of low protein hydrolysis degree, low small molecule peptide content, low survival number of probiotics and poor product stability of a traditional vegetable protein fermentation process. The process comprises the following steps: raw material pretreatment: performing ultrasonic-assisted crushing to prepare a suspension and adjusting the pH value; ultrasonic-assisted enzymolysis: adding a compound enzyme preparation and carrying out ultrasonic treatment; enzyme deactivation; fermentation strain preparation: culturing aspergillus oryzae, lactobacillus plantarum and streptococcus thermophilus composite strains; performing segmented temperature-controlled fermentation: performing fermentation at 28-30 DEG C for 12-16 hours, and then performing fermentation at 33-35 DEG C for 8-12 hours; and post-processing. Through the synergistic effect of ultrasonic-assisted crushing, ultrasonic-assisted enzymolysis and segmented temperature-controlled fermentation, the protein hydrolysis degree is larger than or equal to 45%, the proportion of small molecule peptide is higher than 25%, the survival number of probiotics is larger than or equal to 1 * 10 < 6 > CFU / g, the nutritive value and fermentation efficiency of the product are remarkably improved, and the process is stable and easy to industrialize.

The invention discloses a method for preparing low-sensitization protein peptide through microwave combined enzymolysis composite modification and application, and relates to the technical field of food processing. The treatment method comprises the following steps: preparing protein powder into a solution, adding tea polyphenol into the solution, and uniformly mixing to prepare a protein solution; carrying out microwave modification treatment on the protein solution; adding compound protease into the protein solution subjected to microwave treatment for enzymolysis and enzyme deactivation; and filtering the enzymolysis product, and drying the filtrate to obtain the low-sensitization protein peptide. According to the method, microwave and enzymolysis combined composite modification is adopted, so that the sensitization of protein can be reduced, the reaction time is shortened, and the method is simple in process, free of pollution and high in safety. In the enzymolysis process, active peptide substances which are easy to digest and absorb, can enhance immunity and delay senescence are synchronously generated, and tea polyphenol added in the formula can synergistically relieve anaphylactic reaction and play an anti-oxidation role, so that multi-element synergy of desensitization, function enhancement and flavor optimization is realized, and the added value and market application potential of the product are improved.

The application belongs to the technical field of food processing, and particularly relates to an egg powder and a preparation method thereof. In view of the problems of low protein content and poor brewing performance of the egg powder prepared by the prior art, the egg liquid is first subjected to protease hydrolysis treatment, sugar addition and dispersion treatment to obtain high-temperature-resistant egg liquid, so as to improve the sterilization temperature of the egg liquid and ensure that the egg liquid does not form gel and does not produce fishy smell; then, the acid substance is added to the high-temperature-resistant egg liquid, and the high-temperature-resistant egg liquid is subjected to spray drying treatment after high-temperature short-time sterilization, so as to avoid the increase of the viscosity of the high-temperature-resistant egg liquid after sterilization, and the protein content and the brewing performance of the prepared egg powder are both significantly improved.

The application discloses a pleurotus eryngii enzyme and a preparation method and application thereof. The application takes pleurotus eryngii as raw material, utilizes lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis for co-fermentation, and adopts a semi-solid fermentation in stages to prepare the pleurotus eryngii enzyme. After the fermentation treatment, the release and hydrolysis degree of the protein in the pleurotus eryngii are improved, the contents of soluble protein, amino acid and taste nucleotide are increased, meanwhile, the mushroom smell and the bad flavor of earthy smell are weakened, and flavor characteristics such as fruit aroma and flower aroma are formed. Compared with the existing processing method of the pleurotus eryngii, the application promotes the transformation and release of effective components in the pleurotus eryngii through the composite probiotic fermentation, solves the problems of low protein utilization rate and insufficient flavor quality in the processing of the pleurotus eryngii, improves the nutritional quality and flavor characteristics of the product, and enhances the antioxidant activity. The pleurotus eryngii enzyme prepared through the above method has good processing adaptability, and can be applied to the fields of high-protein food, fresh-taste condiment and functional food.

The application discloses alfalfa type non-antibiotic low-protein daily ration for reducing pig nitrogen emission and a preparation method thereof, which is composed of the following components in terms of dry matter weight fractions: corn 48-59 parts, soybean meal 1-5 parts, bran 1-2 parts, alfalfa powder 15 parts, wheat 16-23 parts, soybean oil 4-5 parts, calcium hydrogen phosphate 0.7-0.9 parts, stone powder 0-0.03 parts, salt 0.3 parts, lysine 0.3-0.5 parts, methionine 0.06-0.07 parts, threonine 0.14-0.21 parts, tryptophan 0.02-0.06 parts, valine 0.05-0.2 parts, isoleucine 0.04-0.2 parts, premix 1 part and compound probiotics 1 part. The daily ration improves the growth performance and meat quality of fattening pigs, improves feed conversion rate and economic benefits, reduces serum urea nitrogen content, promotes nitrogen metabolism of the organism, reduces glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase activities in the liver and improves the immune level of the organism, greatly solves the problem of protein feed resource shortage, reduces emission of urine nitrogen, fecal nitrogen and total nitrogen, guarantees food safety and reduces breeding pollution.

The present application relates to the technical field of livestock feed, and discloses a low-protein feed preparation method for laying hens based on net energy and mineral regulation, which comprises a basic daily ration, a natural intestinal health promoting mineral compound and a functional additive, the basic daily ration is set to a low net energy level, and the sodium-potassium-chlorine electrolyte balance value is strictly controlled, the compound lipid emulsified energy source microcapsule powder in the functional additive releases medium-chain fatty acids in the early stage of the intestinal tract, and the medium-chain fatty acids are absorbed synchronously with free amino acids to improve the utilization rate of crude protein and reduce the corruption of the posterior intestinal tract, activated montmorillonite and natural clinoptilolite are used to cooperatively regulate the osmotic pressure of the intestinal tract, reduce the incidence of watery feces and ammonia emission, L-cystine is crosslinked in the oviduct to form a disulfide bond network skeleton, the expansion effect of selenium-rich tourmaline powder on microvessels is combined, the compression strength of the eggshell and the selenium content of the yolk are effectively improved, and the present application significantly reduces the feed-to-egg ratio, improves the intestinal health and the quality of the end product.

The application relates to the technical field of medical dressings, and discloses a collagen sheet dressing and a preparation method thereof. The collagen sheet dressing comprises fibrous collagen, an auxiliary component, an excipient and a protective agent; wherein the solid content of the fibrous collagen in the composition is greater than 10 mg / ml. The preparation method of the collagen sheet dressing comprises the following steps: preparing a homogenate; drying the homogenate to obtain a dry product; adjusting the humidity of the dry product and irradiating the dry product to form the collagen sheet dressing. Through the above method, the problems of large porosity, low protein concentration and fast degradation speed of the existing products can be solved. In addition, the preparation method of the collagen component provided by the application has cost advantages, and the process is simple and easy to mass-produce.

The invention belongs to the technical field of bioengineering, and particularly relates to a recombinant CSA / FSA protein as well as a preparation method and application thereof. The recombinant CSA / FSA protein is obtained by performing specific site mutation on an amino acid sequence of the dog / cat serum albumin, constructing a recombinant expression vector and expressing a recombinant host, the recombinant CSA / FSA protein is stable in source, relatively low in cost and high in safety, the performance is optimized through mutation, the half-life period is prolonged, and the recombinant CSA / FSA protein also has degradation resistance and can be used for preparing the recombinant CSA / FSA protein. Compared with natural serum albumin, the serum albumin provided by the invention has more excellent performance, and can better exert physiological functions of maintaining blood osmotic pressure, transporting nutrient substances, detoxifying, regulating immunity and the like in dogs and cats. The traditional Chinese medicine composition has important clinical application value and market prospect in the application of treating shock, hypoproteinemia and related diseases caused by factors such as blood loss or burn.

This invention discloses the application of glycine / glutamine enriched sequences in enhancing xylanase activity. The application of the glycine / glutamine enriched sequence shown in SEQ ID NO.1 in enhancing the xylanase activity of BCG1 and its homologous proteins is described. The xylanase activity of the originating BCG1 protein is enhanced by incorporating the glycine / glutamine enriched sequence shown in SEQ ID NO.1 into the C-terminus of a BCG1 protein lacking the glycine / glutamine enriched sequence; or the xylanase activity of the BCG1 protein is reduced by knocking out the glycine / glutamine enriched sequence of a BCG1 protein with xylanase activity. This finding indicates that glycine / glutamine enriched sequences have significant application potential in agricultural production for enhancing xylanase activity.

The invention belongs to the technical field of biological agents and feed, and discloses a biological agent and application thereof in low-protein feed. The core component of the biological agent is rumen bypass glycine (RPGly), and the biological agent is micro-capsule type particles and is composed of an inner layer coating composed of a glycine capsule core, hydrophobic beewax and hydrogenated castor oil, and an outer layer coating containing chitosan and nano silicon dioxide. The preparation has excellent rumen bypass rate and small intestine release rate, and can be cooperatively used with rumen bypass methionine. When being applied to low-protein feed for ruminants, the low-protein feed additive can regulate and control metabolic pathways such as glyceryl phospholipids and glutathione, so that nitrogen emission is remarkably reduced, the growth performance of animals is maintained, the eye muscle area is remarkably increased, the tenderness and flavor of meat are improved, and quality and efficiency improvement in a low-protein breeding mode is realized.

This invention relates to the field of rubber glove production technology, specifically to a low-protein glove production equipment and its production process, including a frame and a mold. Five operating chambers are formed in the middle of the upper surface of the frame. Heating elements are welded to the lower surface of the inner wall of each operating chamber. A support frame is provided between two of the moving frames. Several electromagnetic moving frames are evenly arranged within the support frame. Each electromagnetic moving frame contains an electric telescopic rod, and an electromagnetic slide rail matching the electromagnetic moving frame is provided around the electric telescopic rod. A top frame is welded to the top of the electric telescopic rod. Through the corresponding process, the equipment is integrally formed during processing. The moving mechanism and angle adjustment structure, together with its lifting mechanism, realize the process and effect of mold movement and processing within the equipment, avoiding the cumbersome step-by-step manual operation required in existing technologies.

The application discloses a KASP molecular marker related to quality traits of Brassica napus and application thereof, and belongs to the technical field of molecular genetics.The application discloses the KASP molecular marker related to quality traits of Brassica napus and a primer thereof, and application of the KASP molecular marker and the primer in genotyping or quality trait identification of Brassica napus.The results show that the application can accurately identify genotypes, has high sensitivity and specificity, and effectively reduces the error rate.The application further discloses a Brassica napus genotyping / quality identification method.The results show that a material with a genotyping result of AA has the characteristics of low erucic acid, low glucosinolate, high oleic acid, high linoleic acid, low arachidic acid and low protein content; a material with a GG type shows high erucic acid, high glucosinolate, low oleic acid, low linoleic acid, high arachidic acid and high protein content.The application can quickly screen out individuals with excellent quality traits in early generations, and avoids the long process of identifying traits after plants mature in traditional breeding.