280 results about "Low protein" patented technology

The invention provides a method for preparing recombinant human albumin with low O-glycosylation level, comprising taking at least one measure to improve the growth rate of the strain and reduce the O-glycosylation level of the protein during the fermentation process, and specifically comprising S1, seed liquid preparation; S2, BSM culture medium is put into a fermentation tank, the pH is adjusted to 5.0-5.8, sterilization is carried out, dissolved oxygen is corrected after cooling, temperature and ventilation volume are set, sterilized PTM1 solution is added to the culture medium, and the pH of the culture medium is adjusted to 5.0-5.8 again; S3, shaking flask seeds are connected to the fermentation tank, dissolved oxygen is corrected, and rotation speed is set; S4, when the dissolved oxygen increases suddenly, a glycerol feeding stage is entered, and glycerol feeding is added at a uniform speed; S5, glycerol feeding and methanol feeding are added simultaneously, pH is adjusted to 6.0-6.5, and the temperature is lowered; S6, methanol feeding is added; S7, after the fermentation is completed, the tank is released, the fermentation product is centrifuged, and the supernatant is collected; the method of the invention can reduce protein O-glycosylation, and the expressed recombinant protein contains a very low level of glycosylation modification.

The invention discloses a dCAPS molecular marker for rapidly identifying the content of soybean protein and application of the dCAPS molecular marker. An upstream primer sequence of a primer pair for amplifying the dCAPS molecular marker is as shown in SEQ ID No. 1, and a downstream primer sequence is as shown in SEQ ID No. 2. According to the invention, the mispairing base primer is introduced to carry out PCR amplification and enzyme digestion on the to-be-detected soybean genome DNA, and the soybean protein content can be identified according to the band size of the enzyme digestion product. The dCAPS molecular marker disclosed by the invention can be used for identifying or assisting in identifying the soybean protein content, a high-protein soybean variety and a low-protein soybean variety are distinguished from the molecular level, and the dCAPS molecular marker has important significance on soybean molecular marker assisted breeding and high-protein soybean screening and cultivation.

The invention discloses functional factor-loaded bi-crosslinked food gel and a preparation method thereof, and belongs to the technical field of food processing. The preparation method comprises the following steps: by taking protein, edible oil and fat, kappa-carrageenan, gellan gum and the like as main gel base materials, firstly, inducing by adopting potassium ions to form protein-kappa-carrageenan or protein-gellan gum composite gel; and dipping the composite gel in an acid solution, and inducing to form the bi-crosslinked protein-kappa-carrageenan or protein-gellan gum composite gel. The method can be used for preparing protein-kappa-carrageenan or protein-gellan gum composite hydrogel and composite emulsion gel, can be used for preparing double-crosslinking gel at lower protein and polysaccharide concentration, and can obviously improve the hardness of the gel, reduce the swelling rate of the gel and improve the stability of the gel. The prepared double-crosslinking composite gel can realize co-embedding of hydrophilic and hydrophobic functional factors, and the stability and the controlled release characteristic of the functional factors are remarkably improved.

The invention discloses preparation of plantain herb polysaccharide and application of plantain herb polysaccharide in preparation of medicine for preventing and treating fatty liver hemorrhagic syndrome of laying hens, and belongs to the technical field of biological medicine. The invention also provides application of the plantain herb polysaccharide in preparation of a feed additive for regulating and / or treating fatty liver of laying hens. According to the application disclosed by the invention, through in-vivo and in-vitro experimental modeling and detection of related indexes, the prevention and treatment effects of plantain herb polysaccharides with different doses on laying hen fatty liver caused by high-energy low-protein daily ration are comprehensively discussed, and hepatic lipid deposition is reduced by regulating hepatic lipid synthesis and oxidative catabolism as well as ferroptosis related signal channels; therefore, the laying hen fatty liver hemorrhagic syndrome induced by high-energy low-protein daily ration is effectively prevented and treated. The invention also researches the prevention and treatment effects of the plantain herb polysaccharide on the egg yield of laying hens and the fatty liver, and finds that the plantain herb polysaccharide can improve the egg yield of the laying hens and improve the production performance.

The invention belongs to the technical field of collagen peptide extraction, and discloses a preparation process of low-fishy-smell marine fish skin collagen peptide, which is used for solving the problems of incomplete fishy smell removal and high protein loss rate in the traditional fishy smell removal process. Comprising the following steps: (1) immersing fish skin into water, adding lipase, cleaning the fish skin with pure water, and homogenizing to obtain homogenate; (2) adding saccharomyces cerevisiae and purple perilla juice into the homogenate, carrying out heat preservation and enzymolysis at 30 DEG C, then heating to 45 DEG C, adding compound enzyme for enzymolysis, and heating for enzyme deactivation to obtain collagen peptide enzymatic hydrolysate; (3) adding activated carbon and diatomite into the collagen peptide enzymatic hydrolysate, keeping the temperature at 70 DEG C, stirring, and carrying out filter pressing and refined filtration to obtain a collagen peptide decoloring solution; (4) sterilizing and concentrating the collagen peptide decoloring solution to obtain a collagen peptide concentrated solution; and (5) drying the collagen peptide concentrated solution to obtain collagen peptide powder. The collagen peptide prepared by the method is almost free of fishy smell and low in protein loss.

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.

The invention relates to the technical field of biological separation materials, and discloses an amine-functionalized magnetic nano composite material and application thereof in protein separation and purification. The material has a core-shell structure and comprises a magnetic ferroferric oxide nano core, an ordered mesoporous silicon dioxide intermediate layer and a covalent grafted polyethyleneimine functional layer. By controlling the matching relationship between the pore diameter of mesopores and the molecular size of the polymer and adopting a crosslinking strategy, the material has high magnetic responsiveness, rapid mass transfer performance and high-density surface amino groups. The material shows high adsorption capacity, rapid adsorption kinetics and good cycle stability in the protein separation process, and is suitable for enrichment purification and metal chelating affinity separation of protein in a biological sample. According to the invention, the technical problems of low protein adsorption capacity, slow dynamics and poor cycling stability caused by compact shell layer, non-uniform amino distribution and limited mass transfer of the existing amine functionalized magnetic material are solved.

The invention belongs to the technical field of protein expression, and particularly relates to recombinant elastin with repeated amino acid sequences and a preparation method thereof.The recombinant elastin is composed of 327 AA sequence units which are repeatedly connected in series twice and a spider silk protein GAAn module, and the AA sequence units are derived from human elastin containing VPGVG peptide fragment repeating units; the invention also discloses a double-repeat tandem sequence protein obtained by serially connecting AA sequence units twice, a coding gene of the double-repeat tandem sequence protein, a recombinant elastin sequence, a coding gene of the recombinant elastin sequence, a high-expression vector and a preparation method, and the problems of low protein expression efficiency and high purification cost at present are solved.

The invention discloses an SNP molecular marker related to growth of macrobrachium rosenbergii under the condition of ingestion of low-protein feed and application of the SNP molecular marker, and relates to the field of genetic breeding of macrobrachium rosenbergii. A nucleotide sequence containing the SNP molecular marker is shown as SEQ ID NO.1, the nucleotide sequence is located at the 501st site from the 5'end on the SEQ ID NO.1 and corresponds to the 76447836th site on the 10th chromosome of the macrobrachium rosenbergii, and the polymorphic site of the SNP molecular marker is C or T. The SNP molecular marker is closely related to the body weight and body length of the macrobrachium rosenbergii under the condition of ingestion of the low-protein feed, so that the locus can provide reference for screening and cultivating new varieties of the macrobrachium rosenbergii resistant to the low-protein feed.

The invention discloses purple perilla plant milk rich in strontium element and a preparation process thereof. The purple perilla plant milk is prepared from the following raw materials: shelled purple perilla seeds, shelled flaxseeds, peeled cyperus esculentus, sesame, soybeans, peas and strontium-rich water. The preparation method mainly comprises the following steps: raw material pretreatment, fine wet grinding, filtering and deslagging, high-pressure homogenization and refinement, ultrahigh-temperature instantaneous sterilization and sterile filling. The method ensures the fine taste, stability and safety of the product. The plant milk is rich in strontium element and high-quality plant protein, does not contain lactose and cholesterol, and has the advantages of bone health maintenance, low sensitivity and the like. The problems that casein allergy and lactose intolerance risks exist in traditional animal milk, and existing plant milk is low in protein content, unbalanced in nutrition, dependent on additives and single in function are solved.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

To provide a stainproof member capable of exhibiting both low protein adsorption performance and service durability.SOLUTION: Provided is a stainproof member with a membrane disposed on a substrate. The membrane has a condensate of a hydrolysate of a silane coupling agent or a condensate of a phosphonic acid derivative. The membrane has no halogen atoms, and in water at pH=7, a zeta potential of the membrane is -10.0 mV or more and 10.0 mV or less. When an amount of C atoms (atomic%) / an amount of atoms of an element X that is most abundant on the surface of the substrate (atomic%) is defined as A in an X-ray photoelectron spectroscopy analysis (ESCA measurement) on the substrate, and an amount of C atoms (atomic%) / an amount of atoms of the element X (atomic%) is defined as B in the X-ray photoelectron spectroscopy analysis (ESCA measurement) on the membrane of the substrate is defined as B, the value of B / A is 2.0 or more and 5.0 or less.SELECTED DRAWING: None

Disclosed in the present invention is the use of a substance that reduces the activity or content of the protein TST3b, or a substance that inhibits or reduces the expression of a gene encoding the protein TST3b. In particular, the TST3b protein may be a protein of A1), A2) or A3): A1) a protein having the amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein that is obtained by means of the substitution and / or deletion and / or addition of one or more amino acid residues in the protein of A1), and has 90% or more identity to and the same activity as the protein as shown in A1); A3) a fusion protein obtained by means of linking a protein tag at the N-terminus or / and C-terminus of A1) or A2). The TST3b protein and biological material related thereto can be used to regulate the content of soluble sugar in and the single fruit weight of Solanum lycopersicum.

The invention provides a post-treatment method of single-cell protein fermentation liquor and a fermentation method of single-cell protein thalli. Nuclease and cell wall lytic enzyme are respectively added into fermentation liquor containing single-cell protein thalli to treat the thalli, so that nucleic acid and cell wall residues can be reduced, and the problems that in a traditional fermentation process, the application of the thalli in food is limited due to the high nucleic acid content of the thalli, and the protein extraction efficiency is reduced due to the existence of the cell walls are solved. In addition, by optimizing fermentation conditions, the protein content of the single-cell protein is remarkably increased. The process is simple, low in cost, suitable for industrial production and capable of being widely applied to the fields of feed, food and medicine.

The present invention belongs to the field of traditional Chinese medicines, and particularly relates to Leixihaokang, a preparation method therefor, and use thereof. The Leixihaokang of the present invention is prepared from the following active pharmaceutical ingredients in parts by weight: 10 parts of Tripterygium glycosides and 0.1-1.3 parts of Artemisia annua extract. According to the present invention, animal experiments prove that the Leixihaokang can ameliorate renal damage in nephrotic syndrome caused by adriamycin, reduce the occurrence of proteinuria, and reduce the apoptosis of renal tissue cells. Meanwhile, the Leixihaokang of the present invention has a good inhibitory effect on renal, hepatic, splenic, and cardiac toxicity in the treatment process of nephrotic syndrome. According to the present invention, animal experiments also prove that the Leixihaokang can ameliorate skin injury in imiquimod-induced psoriasis, reduce inflammatory infiltration and alleviate skin damage. Meanwhile, the Leixihaokang has a certain degree of inhibitory effect on the hepatic and reproductive system toxicity in the treatment process of psoriasis.

The invention discloses an improved self-induction culture medium and a culture process, and belongs to the technical field of bioengineering. The improved self-induction culture medium is prepared from 10 g / L of glucose, 3-5 g / L of glycerin, 3 g / L of lactose, 24 g / L of yeast extract, 8 g / L of casein hydrolysate, 0.02-0.05 mM of malonic acid, 1 mM of methionine and 1-3 mM of MgSO4, a phosphate buffer solution is added to control the pH to be 7.2, and the improved self-induction culture medium is prepared by mixing after sterilization. Cells are inoculated to an improved self-induction culture medium for culture, automatic switching of growth-induction stages can be realized based on a pH and DO linkage dynamic feeding control method, the operation complexity is reduced, and the controllability is higher. The culture medium does not need a special inducer, cost is reduced, and protein expression quantity is improved.

The present invention relates to binding molecules comprising an optimised Fc domain, wherein the optimised Fc domain comprises one or more mutations which decrease protein A binding, increase protein A binding, or improve stability. The present invention also relates to methods of purifying heterodimeric proteins comprising an optimised Fc domain comprising one or more mutations which decrease protein A binding or increase protein A binding.

The invention discloses a model and a method for evaluating the demand amount of standard ileum digestible lysine suitable for lactating sows under a low-protein daily ration level. The evaluation model comprises a broken line linear model and a broken line quadratic model; the broken line linear model is xlt; when R is, y = L + U * (R-x); when x is greater than or equal to R, y is equal to L + U * 0, and the quadratic model of the broken line is xlt; when R is, y = L + U * (R-x) 2; when x > = R, y = L + U * 0; wherein y is a sensitive effect index, and x is a standard ileum digestible lysine level; r is a suitable standard ileum digestible lysine level; l is an intercept; u is a slope; % is the mass percentage content.

The present invention discloses a polyethersulfone membrane with low protein adsorption function and its preparation method. The polyethersulfone membrane comprises the following raw materials according to mass percentage: 10-30% of polyethersulfone, 3-15% of amphiphilic copolymer, 2-10% of pore former, 0.5-15% of non-solvent, and 55-75% of solvent. Through a carefully designed formulation and preparation process, the present invention successfully prepares a polyethersulfone membrane with low protein adsorption function. The addition of the amphiphilic copolymer P(PVP-r-PDMS) not only enhances the hydrophilicity and protein adsorption resistance of the membrane, but also realizes the optimization of the membrane structure through the fine regulation of the RTIPS method. Finally, a polyethersulfone hollow fiber membrane with excellent anti-fouling performance and high permeability is obtained, which has broad application prospects in the fields of hemodialysis, drug filtration, food industry, etc.

The invention discloses a method for improving the characteristics of a rapeseed protein-quercetin compound through cooperation of polysaccharide and gelation, and belongs to the field of food additive technology development and food safety control. According to the method, a modified rapeseed protein-quercetin compound is used as a raw material, sodium carboxymethyl cellulose and chitosan are used for regulating and controlling the binding process of protein and quercetin, then nano-gelatinization is used for inducing recombination of compound molecules, and the charge polysaccharide nano-nano-gelatinized rapeseed protein-quercetin compound is obtained. The new technology disclosed by the invention not only can effectively reduce the co-dissolution pH value of the protein and the quercetin, but also can improve the functional characteristics of the quercetin solubilizing effect, the oxidation resistance, the emulsifying characteristic and the like of the compound. The method has the characteristics of simplicity in operation and easiness in amplification, and a new resource can be provided for application of the rapeseed protein-polyphenol compound in the field of emulsion food.

The invention discloses a low-protein feed containing micropterus salmoides polypeptide and application of the low-protein feed. The amino acid sequence of the micropterus salmoides polypeptide is as shown in SEQ ID NO. 1. The micropterus salmoides polypeptide MSNP combined fermented soybean meal is added into the feed, so that the capability of the micropterus salmoides for digesting the feed and absorbing and utilizing nutrient substances is improved, the growth and weight increment of the micropterus salmoides are promoted, the total protein content of the feed and the addition amount of fish meal in the feed are reduced, the feed cost is saved, the culture benefit is improved, and the economic benefit is increased. The pollution pressure of a culture water body is relieved, and the method has wide popularization and application values in the largemouth bass culture industry.

The invention relates to the field of functional food engineering, in particular to low-protein and high-energy baked food as well as a preparation method and application thereof. The low-protein and high-energy baked food is prepared from the following raw materials by mass: 15 to 25 g of sweet potato starch, 15 to 25 g of corn starch, 15 to 25 g of potato starch, 15 to 25 g of pea starch, 35 to 45 g of eggs, 4 to 6 g of corn oil, 8 to 12 g of linseed oil, 0.5 to 1.2 g of haematococcus pluvialis powder, 0.1 to 0.3 g of sodium ascorbate, 15 to 25 g of milk, 10 to 14 g of carrot juice, 18 to 24 g of tomato juice, 5 to 8 g of cane sugar and 5 to 8 g of a flavoring agent. The product disclosed by the invention is relatively low in cost, low in protein and high in energy, adopts different fillings to improve the taste, and has a health-care effect aiming at patients with nephropathy.

The invention relates to protein powder compositions suitable for preparing a food or beverage comprising a lipid that reduces the foaming properties of the protein when the protein is mixed with a liquid or agitated in the presence of a liquid, methods for making the same and food or beverage products comprising the same. The invention also relates to the use of such a lipid as an anti-foaming agent.

The present invention relates to a Single Nucleotide Polymorphism (SNP) marker for determining the degree of protein content of rice seeds and a method for determining the degree of protein content of rice seeds using said marker. Specifically, the present invention relates to an SNP marker for determining protein content and a method for determining protein content using said marker, which constructs a machine learning-based rice protein content prediction model by utilizing SNP markers associated with high and low protein content, thereby enabling the prediction of rice seeds with high protein content in the early stages of growth.

This invention pertains to the purification of microbial fermentation broth, specifically a method for purifying fermentation broth containing β-1,3 / α-1,3-glucan. The method involves ethanol extraction and filtration to extract β-1,3 / α-1,3-glucan, including fermentation broth pretreatment, pH adjustment of the clarified broth after filtration, at least two ethanol extractions and filtrations, and drying and pulverizing the resulting fibrous material to obtain β-1,3 / α-1,3-glucan. This invention solves the problems of low extraction rate and low product purity in existing technologies, offering advantages such as high extraction rate and product yield, low protein and impurity content, and low production cost.

The invention provides high-whiteness and low-isoflavone-content soybean protein isolate for infant food, and belongs to the technical field of infant complementary food. Comprising the following components in percentage by mass: 92.5%-6% of soybean protein isolate, 6.0% of maltodextrin, 0.8% of sodium carboxymethyl cellulose, 0.1% of sucralose and 0.6% of ethyl vanillin, the risk of residual chemical bleaching agents (hydrogen peroxide and sodium hypochlorite) is avoided, the content of isoflavone is reduced to be less than or equal to 10mg / kg, the hidden danger of endocrine interference is reduced, the damage of high temperature and strong acid / alkali to a protein structure is reduced, and the quality of the protein is improved. The energy consumption is reduced through low-temperature sterilization and vacuum freezing dehydration, the whiteness is greater than or equal to 85%, the sensory requirements are met, the protein content is greater than or equal to 90%, the purity is high, and the protein is suitable for being used as a high-quality protein source of infant food; the green solvent and physical / biological decoloration reduce chemical pollution, and the process is more environment-friendly.

The present invention discloses the preparation of plantain polysaccharide and its application in the preparation of drugs for preventing and treating fatty liver and hemorrhagic syndrome in laying hens, belonging to the field of biomedicine technology. The present invention also provides the application of plantain polysaccharide in the preparation of feed additives for regulating and / or treating fatty liver in laying hens. The present invention comprehensively explores the preventive and therapeutic effects of different doses of plantain polysaccharide on fatty liver in laying hens induced by high-energy and low-protein diets through in vitro and in vivo experimental modeling and detection of relevant indicators, and reduces liver fat deposition by regulating liver fat synthesis and oxidative catabolism and ferroptosis-related signaling pathways, thereby effectively preventing and treating fatty liver and hemorrhagic syndrome in laying hens induced by high-energy and low-protein diets. The present invention also studies the preventive and therapeutic effects of plantain polysaccharide on egg production and fatty liver in laying hens, and concludes that plantain polysaccharide can increase egg production in laying hens and improve production performance.

This invention discloses a functional peptide for increasing recombinant protein yield, the sequence of which is shown in any of SEQ ID No. 1-15. The encoding gene and its applications are also disclosed, along with corresponding methods for increasing protein yield. The functional peptide of this invention can significantly increase the yield of recombinant proteins, significantly reduce the loss rate of recombinant proteins during expression and purification in the MtuΔI-CM protein expression system, reduce the production cost of recombinant proteins, and improve the stability of the target protein. It is suitable for the production of various recombinant human proteins, laying the foundation for large-scale, low-cost mass production of various recombinant proteins and possessing good commercial application prospects.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.