149 results about "Low protein" patented technology

The invention relates to the technical field of biological separation materials, and discloses an amine-functionalized magnetic nano composite material and application thereof in protein separation and purification. The material has a core-shell structure and comprises a magnetic ferroferric oxide nano core, an ordered mesoporous silicon dioxide intermediate layer and a covalent grafted polyethyleneimine functional layer. By controlling the matching relationship between the pore diameter of mesopores and the molecular size of the polymer and adopting a crosslinking strategy, the material has high magnetic responsiveness, rapid mass transfer performance and high-density surface amino groups. The material shows high adsorption capacity, rapid adsorption kinetics and good cycle stability in the protein separation process, and is suitable for enrichment purification and metal chelating affinity separation of protein in a biological sample. According to the invention, the technical problems of low protein adsorption capacity, slow dynamics and poor cycling stability caused by compact shell layer, non-uniform amino distribution and limited mass transfer of the existing amine functionalized magnetic material are solved.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The invention discloses a method for improving the characteristics of a rapeseed protein-quercetin compound through cooperation of polysaccharide and gelation, and belongs to the field of food additive technology development and food safety control. According to the method, a modified rapeseed protein-quercetin compound is used as a raw material, sodium carboxymethyl cellulose and chitosan are used for regulating and controlling the binding process of protein and quercetin, then nano-gelatinization is used for inducing recombination of compound molecules, and the charge polysaccharide nano-nano-gelatinized rapeseed protein-quercetin compound is obtained. The new technology disclosed by the invention not only can effectively reduce the co-dissolution pH value of the protein and the quercetin, but also can improve the functional characteristics of the quercetin solubilizing effect, the oxidation resistance, the emulsifying characteristic and the like of the compound. The method has the characteristics of simplicity in operation and easiness in amplification, and a new resource can be provided for application of the rapeseed protein-polyphenol compound in the field of emulsion food.

The invention relates to protein powder compositions suitable for preparing a food or beverage comprising a lipid that reduces the foaming properties of the protein when the protein is mixed with a liquid or agitated in the presence of a liquid, methods for making the same and food or beverage products comprising the same. The invention also relates to the use of such a lipid as an anti-foaming agent.

The present invention relates to a Single Nucleotide Polymorphism (SNP) marker for determining the degree of protein content of rice seeds and a method for determining the degree of protein content of rice seeds using said marker. Specifically, the present invention relates to an SNP marker for determining protein content and a method for determining protein content using said marker, which constructs a machine learning-based rice protein content prediction model by utilizing SNP markers associated with high and low protein content, thereby enabling the prediction of rice seeds with high protein content in the early stages of growth.

This invention pertains to the purification of microbial fermentation broth, specifically a method for purifying fermentation broth containing β-1,3 / α-1,3-glucan. The method involves ethanol extraction and filtration to extract β-1,3 / α-1,3-glucan, including fermentation broth pretreatment, pH adjustment of the clarified broth after filtration, at least two ethanol extractions and filtrations, and drying and pulverizing the resulting fibrous material to obtain β-1,3 / α-1,3-glucan. This invention solves the problems of low extraction rate and low product purity in existing technologies, offering advantages such as high extraction rate and product yield, low protein and impurity content, and low production cost.

The invention provides high-whiteness and low-isoflavone-content soybean protein isolate for infant food, and belongs to the technical field of infant complementary food. Comprising the following components in percentage by mass: 92.5%-6% of soybean protein isolate, 6.0% of maltodextrin, 0.8% of sodium carboxymethyl cellulose, 0.1% of sucralose and 0.6% of ethyl vanillin, the risk of residual chemical bleaching agents (hydrogen peroxide and sodium hypochlorite) is avoided, the content of isoflavone is reduced to be less than or equal to 10mg / kg, the hidden danger of endocrine interference is reduced, the damage of high temperature and strong acid / alkali to a protein structure is reduced, and the quality of the protein is improved. The energy consumption is reduced through low-temperature sterilization and vacuum freezing dehydration, the whiteness is greater than or equal to 85%, the sensory requirements are met, the protein content is greater than or equal to 90%, the purity is high, and the protein is suitable for being used as a high-quality protein source of infant food; the green solvent and physical / biological decoloration reduce chemical pollution, and the process is more environment-friendly.

This invention discloses a functional peptide for increasing recombinant protein yield, the sequence of which is shown in any of SEQ ID No. 1-15. The encoding gene and its applications are also disclosed, along with corresponding methods for increasing protein yield. The functional peptide of this invention can significantly increase the yield of recombinant proteins, significantly reduce the loss rate of recombinant proteins during expression and purification in the MtuΔI-CM protein expression system, reduce the production cost of recombinant proteins, and improve the stability of the target protein. It is suitable for the production of various recombinant human proteins, laying the foundation for large-scale, low-cost mass production of various recombinant proteins and possessing good commercial application prospects.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.

The invention belongs to the technical field of food processing, and discloses a low-GI protein bar and a preparation method thereof. The low-GI protein bar comprises the following raw materials in parts by mass: 32-48 parts of pretreated egg white liquid, 16-24 parts of whole wheat flour, 8-12 parts of oatmeal, 5.5-8.5 parts of bamboo shoot dietary fibers, 1.5-2.5 parts of chia seeds, 7-11 parts of date palm paste, 4.5-7.5 parts of a sweetening agent and 11-17 parts of auxiliary materials, wherein a preparation method of the pretreated egg white liquid comprises the following steps: adding an acid solution into the egg white liquid to adjust the pH value to 6.5-7, and then carrying out protease enzymolysis treatment. The method effectively solves the problems that a traditional protein bar with egg white as a core protein source is obvious in fishy smell, dry, hard and rough in texture, prone to cracking and scaling in appearance and the like, and the prepared low-GI protein bar is excellent in overall sensory quality and has the nutritional characteristics of being high in protein, high in fiber and low in GI.

This invention provides the application of substances targeting the CYTB-187AA gene and / or protein in the preparation of drugs for delaying aging and / or prolonging lifespan. The research results of this invention indicate that by reducing the expression of CYTB-187AA protein and / or inhibiting its activity, the aging process can be effectively delayed, thereby achieving the goal of extending healthy lifespan or lifespan. The research results of this invention are of great significance for scientific research on aging mechanisms and the development of anti-aging intervention strategies.

The invention relates to the technical field of feed production, and discloses a low-protein piglet feed and a preparation method thereof. Comprising the following components in percentage by mass: 40%-60% of corn, 10%-20% of peeled soybean meal, 5%-8% of fermented soybean meal, 3%-10% of low-protein whey powder, 2%-8% of fish meal, 2%-5% of glucose, 1%-3% of soybean oil, 1.0%-2.5% of compound amino acid, 0.5%-1% of monocalcium phosphate, 0.5%-1% of stone powder, 0.1%-0.2% of glutamine, 1% of a vitamin mineral mixture, 0.2%-0.5% of an acidifying agent and 0.1%-0.2% of an enzyme preparation. According to the piglet feed formula, the acidifying agent is accurately added to cooperate with the synthesized amino acid to optimize intestinal health and reduce the content of crude protein, antibiotics are effectively replaced, and the green and environment-friendly breeding requirement in the post-antibiotic era is met.

The invention relates to the technical field of nutritional ingredient analysis, and discloses an ingredient metering system for low-protein nutrition balanced catering, and the system comprises a multi-modal data collection module which is used for obtaining the total mass, color and depth images of to-be-detected meals; the visual guidance spectrum analysis module is used for performing instance segmentation on the color image and guiding a spectrum probe to perform targeted scanning on each independent food entity so as to obtain spectrum data; the metabolic twinning and chemical imaging module is used for calculating and summarizing nutritional ingredients of each food entity by fusing spectrum and image information through a metabolic twinning algorithm model; and the individualized self-adaptive calibration module is used for acquiring the real physiological indexes of the user and updating the model parameters according to the metabolic response deviation so as to realize closed-loop calibration. According to the method, the visual guidance spectrum is combined with the metabolic twinborn model, and closed-loop calibration is carried out by utilizing the physiological indexes, so that accurate measurement and individualized analysis of the nutrition of cooked meals are realized.

The invention aims to provide a method, equipment, medium and program product for determining binding protein information with pH sensitivity, and the method comprises the following steps: determining the binding protein information based on the target protein information, the target amino acid corresponding to the binding region amino acid of the target protein and the noise information of the binding protein in the initial time step; according to a first binding protein generation model, binding protein information corresponding to the target protein is generated, the target amino acid and binding region amino acid of the target protein are matched with preset pH sensitivity information, and binding protein sequence information in the binding protein information contains the target amino acid; the first binding protein generation model includes a first flow matching predictive model. A velocity field from noise data to binding protein information is predicted through a flow matching method, and the pH sensitivity of the binding protein is improved by introducing target amino acid in each step of prediction, so that ideal binding protein information is generated, the experience requirement of a protein designer is reduced, and the design efficiency is improved.

The present disclosure generally relates to compositions and methods for reducing the biological age and / or the rate of biological age increase in adult animals. More specifically, the present disclosure relates to administering a formulation comprising an effective amount of glycine and / or a functional derivative thereof, alone or in combination with a dietary regimen, such as a calorie-restricted diet, an aging diet, or a low-protein diet. The formulation can simultaneously reduce the biological age of a tissue, organ, or physiological system in an adult animal in need thereof; or treat, prevent, or delay the onset of organ disease, or organ function decline, or conditions associated therewith, in an adult animal in need thereof.

The invention discloses an InDel molecular marker closely linked with soybean protein content characters, a detection primer and application, and belongs to the technical field of molecular biology. The nucleotide sequence of the InDel molecular marker is shown as SEQ ID NO.5, the 96th site to the 106th site of the SEQ ID NO.5 are insertion or deletion variation, and the soybean containing the 96th site to the 106th site insertion sequence of the SEQ ID NO.5 is a variety with high protein content; and the soybean containing the 96th-106th deletion sequence of the SEQ ID NO.5 is a variety with low protein content. According to the invention, 11bp insertion / deletion variation at 377 and 973bp positions of the soybean 14 # chromosome is found, and experiments show that the 11bp insertion / deletion variation has remarkable relevance with the soybean protein content. When the gene is applied to molecular assisted breeding, the protein content expression of a soybean candidate material can be predicted at an early stage.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

The present application belongs to the technical field of biological medicine, and particularly relates to a tremella polysaccharide prepared by a low eutectic solvent method and application thereof in preparation of an anti-breast cancer drug. The present application first discovers that the tremella polysaccharide extracted by the low eutectic solvent method has a significant effect of treating breast cancer, and can resist breast cancer through inhibition of cell proliferation, inhibition of cell migration and invasion and other pathways. Compared with the tremella polysaccharide obtained by a traditional water extraction and alcohol precipitation method, the tremella polysaccharide obtained by the low eutectic solvent method has a lower total sugar content and weight average molecular weight, and a slightly higher protein content, and the tremella polysaccharide can not only significantly improve inhibition of breast cancer cell proliferation, but also significantly inhibit migration and invasion of breast cancer cells. This multi-target effect mode can effectively block the source of tumor diffusion, improve the treatment effect and the survival rate of patients, reduce the risk of recurrence and metastasis, and enhance the overall effect of treatment, and has a good application prospect in the treatment of breast cancer.

The invention relates to a wet spinning preparation process of protein modified polyacrylonitrile fibers, and relates to the field of wet spinning preparation processes, the wet spinning preparation process comprises the following steps: S1, heating and softening a spinning stock solution, dropwise adding a protein solution, and fully dissolving to obtain a clear and transparent protein modified spinning solution; s2, the protein modified spinning solution is sequentially subjected to filtering, defoaming, spinning, coagulating bath and drafting, and then the protein modified polyacrylonitrile fiber is obtained; the spinning stock solution is an acrylonitrile-methyl acrylate-itaconic acid ternary copolymerized stock solution. The acrylonitrile-methyl acrylate-itaconic acid ternary copolymerized stock solution is adopted as a spinning matrix, the compatibility with natural protein is improved by introducing a polar group, and the protein is uniformly dispersed at a molecular level in cooperation with a mode of dropwise adding the protein solution after heating and softening, so that the protein agglomeration phenomenon is remarkably reduced, and the uniformity of the internal structure of the fiber is improved.

The application discloses a kind of double crosslinking food gels of functional factor and preparation method thereof, belong to food processing technical field.The application uses protein, edible oil, kappa-carrageenan, gellan gum and the like as main gel base material, first, potassium ion is used to induce the formation protein-kappa-carrageenan or protein-gellan gum composite gel, then composite gel is immersed in acidic solution and induced to form double crosslinking protein-kappa-carrageenan or protein-gellan gum composite gel.The method can be used for the preparation of protein-kappa-carrageenan or protein-gellan gum composite hydrogel and composite emulsion gel, and double crosslinking gel can be prepared at lower protein and polysaccharide concentration, which can significantly improve the hardness of gel, reduce the swelling rate of gel, and the double crosslinking composite gel prepared can realize the co-embedding of hydrophilic and hydrophobic functional factors, significantly improve the stability and controlled release characteristics of functional factors.

The invention provides a preparation process for preparing water-soluble euglena beta-glucan by an enzymolysis method. The process comprises the specific steps of pretreatment of euglena polysaccharide, enzymolysis reaction of the euglena polysaccharide and the like. According to the method disclosed by the invention, multiple enzymes are used for carrying out the enzymolysis reaction together, and key conditions such as the solid-liquid ratio, the enzymolysis time and the enzymolysis temperature in the enzymolysis reaction are controlled, so that the prepared water-soluble euglena beta-glucan is high in purity and low in protein content, and the total sugar content of the water-soluble euglena beta-glucan can reach 60% or above. The euglena beta-glucan is prepared by using an enzymolysis method, the reaction condition is mild, the use of a large number of organic reagents is avoided, the method is safe and environment-friendly, the preparation process is simple, and the production cost is low.

The invention relates to the technical field of livestock breeding and feed nutrition, and discloses a low-protein diversified daily ration design method and system based on multi-source data fusion, and the method comprises the steps: constructing a formula-environment-effect directed causal diagram through a PC algorithm, estimating the effect deviation contribution proportion of an environment factor and a formula factor through anti-factual reasoning, and calculating the effect deviation contribution proportion of the environment factor and the formula factor; and inferring the target weight preference of the farm by using inverse reinforcement learning, and carrying out conditional weight updating according to a causal attribution result. Real reasons of growth effect deviation can be distinguished, effect deviation errors caused by environmental factors are prevented from being attributed to improper formula setting, and the accuracy and stability of formula recommendation are improved.

The invention discloses a preparation method and application of a fat-soluble drug solid dispersion based on a zwitterionic hyperbranched polymer. According to the solid dispersion, a zwitterionic hyperbranched polymer HP-CB is used as a carrier, and a fat-soluble drug is loaded in an internal hydrophobic cavity; the preparation method of the solid dispersion comprises the following steps: loading a fat-soluble drug in a polymer by using a solvent evaporation method, freeze-drying to form the solid dispersion, and further compounding the solid dispersion with a cream matrix to prepare the nanoparticle composite cream. The solid dispersion has excellent hydrophilicity, low protein adsorptivity and good biocompatibility, the drug solubility and the transdermal permeability are remarkably improved, drug crystallization is effectively inhibited, the stability of a preparation is improved, and further prepared cream has the advantages of softening cuticles, promoting hydration and enhancing the sealing performance and has good application prospects. The traditional Chinese medicine composition can effectively improve chronic inflammatory skin diseases such as psoriasis, and has good clinical application prospect and industrialization value.

The application discloses a peanut protein content related molecular marker and related application. The molecular marker is located at 95485614bp-95490150bp of peanut chromosome 1, is an RNA binding protein KH domain-containing protein gene, and a SNP site (T / A) is detected at 95487145bp, namely, the gene is on the complementary strand, the position (CDS sequence 1229th base) on the gene sequence is A in the low-protein peanut group and T in the high-protein peanut group, and a mutation from lysine to isoleucine (protein 410th amino acid) is caused. The application provides a new marker for peanut high-protein resource molecular marker assisted selection for the first time, and has very important significance for high-protein peanut resource screening and high-protein peanut new variety breeding.

The present application relates to the technical field of meal replacement powder, and discloses a high-protein satiety meal replacement powder containing rice and pea protein and a preparation method thereof.The meal replacement powder comprises a protein component, dietary fiber, high-quality oil, a sweetener, trace nutrients, a stabilizer and a flavor component; wherein the protein component is a composite protein powder prepared by homogenizing, high-temperature shearing and spray drying of rice protein and pea protein.The present application utilizes the complementary characteristics of amino acids of rice protein and pea protein to achieve balanced nutrition, low allergenicity and easy digestion; the combination of dietary fiber, high-quality oil and composite trace nutrients takes into account high protein, high satiety and comprehensive nutrition; the product is prepared by a segmented mixing process and has good stability, diverse flavors and convenient preparation, and is suitable for people with lactose intolerance, weight control, exercise and other groups, solving the technical problems of low protein content, high allergenicity, nutritional imbalance and strong beany odor of traditional meal replacement powder, and being suitable for industrialized popularization and application.

The application relates to the glove production technical field, in particular to a condensing equipment for low-protein durable oxford glove production, which comprises a condensing box, a base, a cooling box and a cooling box. The upper end of the condensing box is a slide rail, the upper end of the slide rail is a conveying chain, the conveying chain is provided with a mounting plate, adjacent mounting plates arranged on the conveying chain are fixedly provided with connecting shafts, movable blocks are movably arranged on the connecting shafts, conveying assemblies are arranged at the lower ends of the movable blocks, and oxford gloves are arranged at the lower ends of the conveying assemblies. The condensing equipment for low-protein durable oxford glove production is provided with a nitrogen assembly and a refrigeration assembly which are combined to cyclically and reciprocally deliver the refrigerated air into the cooling box, then the reflux assembly arranged in the cooling box is started, the cooling liquid filled in the condensing box is subjected to heat exchange with the cold air introduced into the cooling box, and then is delivered into the condensing box for use again, so that the cooling liquid does not need to be replaced.

The application discloses a dCAPS molecular marker for rapidly identifying soybean protein content and application thereof. The sequence of the upstream primer of the primer pair for amplifying the dCAPS molecular marker is shown in SEQ ID No. 1, and the sequence of the downstream primer is shown in SEQ ID No. 2. The soybean genomic DNA to be detected is subjected to PCR amplification and enzyme cutting by using the mismatched base primer, and the soybean protein content can be identified according to the band size of the enzyme cutting product. The dCAPS molecular marker can be used for identifying or assisting in identifying the soybean protein content, and can distinguish the high-protein soybean varieties from the low-protein soybean varieties from the molecular level, and has important significance for the soybean molecular marker assisted breeding and the screening and cultivation of high-protein soybeans.

The invention discloses an integrated feeding control method for acetic acid group single-cell protein fermentation and application of the integrated feeding control method, and belongs to the technical field of microbial fermentation. By monitoring the change of the pH value of the fermentation liquid in real time, the composite material supplementing system containing the carbon and nitrogen sources is automatically triggered, pH signals are converted into the supplementing amount of the carbon sources and the nitrogen sources, the stable carbon-nitrogen ratio of supplemented materials in the fermentation process is maintained, and meanwhile the pH value is maintained in an appropriate range. The thallus yield and the protein content of the single-cell protein are obviously improved; the problems of poor thallus growth, low protein content and large substrate residual quantity caused by mutual independence of a carbon source, a nitrogen source and pH adjustment, tedious control of a fermentation process and imbalance of a carbon-nitrogen ratio in an existing material supplementing mode are solved.

The invention discloses a modified ultrafiltration membrane, a preparation method thereof and a method for preparing a protein sample for mass spectrometry by adopting the modified ultrafiltration membrane, and belongs to the technical field of protein sample preparation. The surface of the ultrafiltration membrane is subjected to graft modification, so that the hydrophilicity of the surface of the membrane is effectively enhanced, that is, protein adsorption and membrane pollution are reduced by reducing the hydrophobicity of the surface of the membrane and increasing the hydrophilicity. According to the invention, amphoteric small molecules are connected to cellulose molecules of the ultrafiltration membrane, so that hydrophilic ends face outwards to mutually form hydrogen bonds to improve the hydrophilicity. Specifically, according to the method, the membrane is firstly subjected to alkaline activation, then amphoteric small molecules are inoculated, and the connection efficiency is improved. When the modified material is used for preparing a protein sample, the adsorption effect of the membrane on the protein sample can be reduced, the number of identified proteins and peptide fragments is finally increased, protein loss is reduced in the sample pretreatment step, and identification of more proteins and peptide fragments is facilitated.