222 results about "Low protein" patented technology

The invention belongs to the technical field of collagen peptide extraction, and discloses a preparation process of low-fishy-smell marine fish skin collagen peptide, which is used for solving the problems of incomplete fishy smell removal and high protein loss rate in the traditional fishy smell removal process. Comprising the following steps: (1) immersing fish skin into water, adding lipase, cleaning the fish skin with pure water, and homogenizing to obtain homogenate; (2) adding saccharomyces cerevisiae and purple perilla juice into the homogenate, carrying out heat preservation and enzymolysis at 30 DEG C, then heating to 45 DEG C, adding compound enzyme for enzymolysis, and heating for enzyme deactivation to obtain collagen peptide enzymatic hydrolysate; (3) adding activated carbon and diatomite into the collagen peptide enzymatic hydrolysate, keeping the temperature at 70 DEG C, stirring, and carrying out filter pressing and refined filtration to obtain a collagen peptide decoloring solution; (4) sterilizing and concentrating the collagen peptide decoloring solution to obtain a collagen peptide concentrated solution; and (5) drying the collagen peptide concentrated solution to obtain collagen peptide powder. The collagen peptide prepared by the method is almost free of fishy smell and low in protein loss.

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.

The invention relates to the technical field of biological separation materials, and discloses an amine-functionalized magnetic nano composite material and application thereof in protein separation and purification. The material has a core-shell structure and comprises a magnetic ferroferric oxide nano core, an ordered mesoporous silicon dioxide intermediate layer and a covalent grafted polyethyleneimine functional layer. By controlling the matching relationship between the pore diameter of mesopores and the molecular size of the polymer and adopting a crosslinking strategy, the material has high magnetic responsiveness, rapid mass transfer performance and high-density surface amino groups. The material shows high adsorption capacity, rapid adsorption kinetics and good cycle stability in the protein separation process, and is suitable for enrichment purification and metal chelating affinity separation of protein in a biological sample. According to the invention, the technical problems of low protein adsorption capacity, slow dynamics and poor cycling stability caused by compact shell layer, non-uniform amino distribution and limited mass transfer of the existing amine functionalized magnetic material are solved.

The invention belongs to the technical field of protein expression, and particularly relates to recombinant elastin with repeated amino acid sequences and a preparation method thereof.The recombinant elastin is composed of 327 AA sequence units which are repeatedly connected in series twice and a spider silk protein GAAn module, and the AA sequence units are derived from human elastin containing VPGVG peptide fragment repeating units; the invention also discloses a double-repeat tandem sequence protein obtained by serially connecting AA sequence units twice, a coding gene of the double-repeat tandem sequence protein, a recombinant elastin sequence, a coding gene of the recombinant elastin sequence, a high-expression vector and a preparation method, and the problems of low protein expression efficiency and high purification cost at present are solved.

The invention discloses purple perilla plant milk rich in strontium element and a preparation process thereof. The purple perilla plant milk is prepared from the following raw materials: shelled purple perilla seeds, shelled flaxseeds, peeled cyperus esculentus, sesame, soybeans, peas and strontium-rich water. The preparation method mainly comprises the following steps: raw material pretreatment, fine wet grinding, filtering and deslagging, high-pressure homogenization and refinement, ultrahigh-temperature instantaneous sterilization and sterile filling. The method ensures the fine taste, stability and safety of the product. The plant milk is rich in strontium element and high-quality plant protein, does not contain lactose and cholesterol, and has the advantages of bone health maintenance, low sensitivity and the like. The problems that casein allergy and lactose intolerance risks exist in traditional animal milk, and existing plant milk is low in protein content, unbalanced in nutrition, dependent on additives and single in function are solved.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The invention provides a post-treatment method of single-cell protein fermentation liquor and a fermentation method of single-cell protein thalli. Nuclease and cell wall lytic enzyme are respectively added into fermentation liquor containing single-cell protein thalli to treat the thalli, so that nucleic acid and cell wall residues can be reduced, and the problems that in a traditional fermentation process, the application of the thalli in food is limited due to the high nucleic acid content of the thalli, and the protein extraction efficiency is reduced due to the existence of the cell walls are solved. In addition, by optimizing fermentation conditions, the protein content of the single-cell protein is remarkably increased. The process is simple, low in cost, suitable for industrial production and capable of being widely applied to the fields of feed, food and medicine.

The present invention belongs to the field of traditional Chinese medicines, and particularly relates to Leixihaokang, a preparation method therefor, and use thereof. The Leixihaokang of the present invention is prepared from the following active pharmaceutical ingredients in parts by weight: 10 parts of Tripterygium glycosides and 0.1-1.3 parts of Artemisia annua extract. According to the present invention, animal experiments prove that the Leixihaokang can ameliorate renal damage in nephrotic syndrome caused by adriamycin, reduce the occurrence of proteinuria, and reduce the apoptosis of renal tissue cells. Meanwhile, the Leixihaokang of the present invention has a good inhibitory effect on renal, hepatic, splenic, and cardiac toxicity in the treatment process of nephrotic syndrome. According to the present invention, animal experiments also prove that the Leixihaokang can ameliorate skin injury in imiquimod-induced psoriasis, reduce inflammatory infiltration and alleviate skin damage. Meanwhile, the Leixihaokang has a certain degree of inhibitory effect on the hepatic and reproductive system toxicity in the treatment process of psoriasis.

The invention discloses an improved self-induction culture medium and a culture process, and belongs to the technical field of bioengineering. The improved self-induction culture medium is prepared from 10 g / L of glucose, 3-5 g / L of glycerin, 3 g / L of lactose, 24 g / L of yeast extract, 8 g / L of casein hydrolysate, 0.02-0.05 mM of malonic acid, 1 mM of methionine and 1-3 mM of MgSO4, a phosphate buffer solution is added to control the pH to be 7.2, and the improved self-induction culture medium is prepared by mixing after sterilization. Cells are inoculated to an improved self-induction culture medium for culture, automatic switching of growth-induction stages can be realized based on a pH and DO linkage dynamic feeding control method, the operation complexity is reduced, and the controllability is higher. The culture medium does not need a special inducer, cost is reduced, and protein expression quantity is improved.

The present invention discloses a polyethersulfone membrane with low protein adsorption function and its preparation method. The polyethersulfone membrane comprises the following raw materials according to mass percentage: 10-30% of polyethersulfone, 3-15% of amphiphilic copolymer, 2-10% of pore former, 0.5-15% of non-solvent, and 55-75% of solvent. Through a carefully designed formulation and preparation process, the present invention successfully prepares a polyethersulfone membrane with low protein adsorption function. The addition of the amphiphilic copolymer P(PVP-r-PDMS) not only enhances the hydrophilicity and protein adsorption resistance of the membrane, but also realizes the optimization of the membrane structure through the fine regulation of the RTIPS method. Finally, a polyethersulfone hollow fiber membrane with excellent anti-fouling performance and high permeability is obtained, which has broad application prospects in the fields of hemodialysis, drug filtration, food industry, etc.

The invention discloses a method for improving the characteristics of a rapeseed protein-quercetin compound through cooperation of polysaccharide and gelation, and belongs to the field of food additive technology development and food safety control. According to the method, a modified rapeseed protein-quercetin compound is used as a raw material, sodium carboxymethyl cellulose and chitosan are used for regulating and controlling the binding process of protein and quercetin, then nano-gelatinization is used for inducing recombination of compound molecules, and the charge polysaccharide nano-nano-gelatinized rapeseed protein-quercetin compound is obtained. The new technology disclosed by the invention not only can effectively reduce the co-dissolution pH value of the protein and the quercetin, but also can improve the functional characteristics of the quercetin solubilizing effect, the oxidation resistance, the emulsifying characteristic and the like of the compound. The method has the characteristics of simplicity in operation and easiness in amplification, and a new resource can be provided for application of the rapeseed protein-polyphenol compound in the field of emulsion food.

The invention relates to protein powder compositions suitable for preparing a food or beverage comprising a lipid that reduces the foaming properties of the protein when the protein is mixed with a liquid or agitated in the presence of a liquid, methods for making the same and food or beverage products comprising the same. The invention also relates to the use of such a lipid as an anti-foaming agent.

The present invention relates to a Single Nucleotide Polymorphism (SNP) marker for determining the degree of protein content of rice seeds and a method for determining the degree of protein content of rice seeds using said marker. Specifically, the present invention relates to an SNP marker for determining protein content and a method for determining protein content using said marker, which constructs a machine learning-based rice protein content prediction model by utilizing SNP markers associated with high and low protein content, thereby enabling the prediction of rice seeds with high protein content in the early stages of growth.

This invention pertains to the purification of microbial fermentation broth, specifically a method for purifying fermentation broth containing β-1,3 / α-1,3-glucan. The method involves ethanol extraction and filtration to extract β-1,3 / α-1,3-glucan, including fermentation broth pretreatment, pH adjustment of the clarified broth after filtration, at least two ethanol extractions and filtrations, and drying and pulverizing the resulting fibrous material to obtain β-1,3 / α-1,3-glucan. This invention solves the problems of low extraction rate and low product purity in existing technologies, offering advantages such as high extraction rate and product yield, low protein and impurity content, and low production cost.

The invention provides high-whiteness and low-isoflavone-content soybean protein isolate for infant food, and belongs to the technical field of infant complementary food. Comprising the following components in percentage by mass: 92.5%-6% of soybean protein isolate, 6.0% of maltodextrin, 0.8% of sodium carboxymethyl cellulose, 0.1% of sucralose and 0.6% of ethyl vanillin, the risk of residual chemical bleaching agents (hydrogen peroxide and sodium hypochlorite) is avoided, the content of isoflavone is reduced to be less than or equal to 10mg / kg, the hidden danger of endocrine interference is reduced, the damage of high temperature and strong acid / alkali to a protein structure is reduced, and the quality of the protein is improved. The energy consumption is reduced through low-temperature sterilization and vacuum freezing dehydration, the whiteness is greater than or equal to 85%, the sensory requirements are met, the protein content is greater than or equal to 90%, the purity is high, and the protein is suitable for being used as a high-quality protein source of infant food; the green solvent and physical / biological decoloration reduce chemical pollution, and the process is more environment-friendly.

The present invention discloses the preparation of plantain polysaccharide and its application in the preparation of drugs for preventing and treating fatty liver and hemorrhagic syndrome in laying hens, belonging to the field of biomedicine technology. The present invention also provides the application of plantain polysaccharide in the preparation of feed additives for regulating and / or treating fatty liver in laying hens. The present invention comprehensively explores the preventive and therapeutic effects of different doses of plantain polysaccharide on fatty liver in laying hens induced by high-energy and low-protein diets through in vitro and in vivo experimental modeling and detection of relevant indicators, and reduces liver fat deposition by regulating liver fat synthesis and oxidative catabolism and ferroptosis-related signaling pathways, thereby effectively preventing and treating fatty liver and hemorrhagic syndrome in laying hens induced by high-energy and low-protein diets. The present invention also studies the preventive and therapeutic effects of plantain polysaccharide on egg production and fatty liver in laying hens, and concludes that plantain polysaccharide can increase egg production in laying hens and improve production performance.

This invention discloses a functional peptide for increasing recombinant protein yield, the sequence of which is shown in any of SEQ ID No. 1-15. The encoding gene and its applications are also disclosed, along with corresponding methods for increasing protein yield. The functional peptide of this invention can significantly increase the yield of recombinant proteins, significantly reduce the loss rate of recombinant proteins during expression and purification in the MtuΔI-CM protein expression system, reduce the production cost of recombinant proteins, and improve the stability of the target protein. It is suitable for the production of various recombinant human proteins, laying the foundation for large-scale, low-cost mass production of various recombinant proteins and possessing good commercial application prospects.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.

The invention belongs to the technical field of food processing, and discloses a low-GI protein bar and a preparation method thereof. The low-GI protein bar comprises the following raw materials in parts by mass: 32-48 parts of pretreated egg white liquid, 16-24 parts of whole wheat flour, 8-12 parts of oatmeal, 5.5-8.5 parts of bamboo shoot dietary fibers, 1.5-2.5 parts of chia seeds, 7-11 parts of date palm paste, 4.5-7.5 parts of a sweetening agent and 11-17 parts of auxiliary materials, wherein a preparation method of the pretreated egg white liquid comprises the following steps: adding an acid solution into the egg white liquid to adjust the pH value to 6.5-7, and then carrying out protease enzymolysis treatment. The method effectively solves the problems that a traditional protein bar with egg white as a core protein source is obvious in fishy smell, dry, hard and rough in texture, prone to cracking and scaling in appearance and the like, and the prepared low-GI protein bar is excellent in overall sensory quality and has the nutritional characteristics of being high in protein, high in fiber and low in GI.

This invention provides the application of substances targeting the CYTB-187AA gene and / or protein in the preparation of drugs for delaying aging and / or prolonging lifespan. The research results of this invention indicate that by reducing the expression of CYTB-187AA protein and / or inhibiting its activity, the aging process can be effectively delayed, thereby achieving the goal of extending healthy lifespan or lifespan. The research results of this invention are of great significance for scientific research on aging mechanisms and the development of anti-aging intervention strategies.

The invention relates to the technical field of feed production, and discloses a low-protein piglet feed and a preparation method thereof. Comprising the following components in percentage by mass: 40%-60% of corn, 10%-20% of peeled soybean meal, 5%-8% of fermented soybean meal, 3%-10% of low-protein whey powder, 2%-8% of fish meal, 2%-5% of glucose, 1%-3% of soybean oil, 1.0%-2.5% of compound amino acid, 0.5%-1% of monocalcium phosphate, 0.5%-1% of stone powder, 0.1%-0.2% of glutamine, 1% of a vitamin mineral mixture, 0.2%-0.5% of an acidifying agent and 0.1%-0.2% of an enzyme preparation. According to the piglet feed formula, the acidifying agent is accurately added to cooperate with the synthesized amino acid to optimize intestinal health and reduce the content of crude protein, antibiotics are effectively replaced, and the green and environment-friendly breeding requirement in the post-antibiotic era is met.

The invention relates to the technical field of nutritional ingredient analysis, and discloses an ingredient metering system for low-protein nutrition balanced catering, and the system comprises a multi-modal data collection module which is used for obtaining the total mass, color and depth images of to-be-detected meals; the visual guidance spectrum analysis module is used for performing instance segmentation on the color image and guiding a spectrum probe to perform targeted scanning on each independent food entity so as to obtain spectrum data; the metabolic twinning and chemical imaging module is used for calculating and summarizing nutritional ingredients of each food entity by fusing spectrum and image information through a metabolic twinning algorithm model; and the individualized self-adaptive calibration module is used for acquiring the real physiological indexes of the user and updating the model parameters according to the metabolic response deviation so as to realize closed-loop calibration. According to the method, the visual guidance spectrum is combined with the metabolic twinborn model, and closed-loop calibration is carried out by utilizing the physiological indexes, so that accurate measurement and individualized analysis of the nutrition of cooked meals are realized.

A method for modifying a polymer membrane comprises pre-wetting the polymer membrane with a cross-linker solution comprising a first cross-linker and a second cross-linker; irradiating the pre-wetted polymer membrane to induce a cross-linking reaction; and rinsing the polymer membrane after the cross-linking reaction and drying it to obtain a modified polymer membrane. The polymer membrane modification method of the present invention utilizes two cross-linking agents to modify the polymer membrane, forming a 3D network on the surface and bulk of the polymer membrane to obtain a modified polymer membrane. The modified polymer membrane of the present invention exhibits low protein adsorption, caustic stability, autoclave stability, and gamma sterilization stability, while retaining overall mechanical properties and meeting the foldability requirements for filter preparation.

A method for preparing recombinant human albumin with high expression and low O-glycosylation level, comprising taking at least one measure during fermentation to improve the protein expression rate and reduce the O-glycosylation level of a protein; and specifically comprising: S1, seed solution preparation; S2, batch fermentation; S3, glycerol fed fermentation; S4, methanol fed fermentation; S5, fermentation product collection.

The present disclosure relates generally to compositions and methods for reducing the biological age and / or reducing the rate of biological age increase in an adult animal. More specifically, the present disclosure relates to administering formulations comprising an effective amount of glycine and / or functional derivatives, alone or in combination with a dietary regimen such as caloric limiting, elderly diet or low protein diet. The preparation can reduce the biological age of tissues, organs or physiological systems in adult animals in need at the same time; or treating, preventing or delaying the onset of organ disease, or the decline of organ function, or a condition associated therewith, in the adult animal in need thereof.

The present invention relates to the field of compound detection technology, and discloses a preparation method of a magnetic MOF composite material and a detection method for chemicals. The magnetic MOF composite material is a core-shell structure, including a magnetic core, an outer metal organic framework structure, and the metal organic framework structure has 2 to 5 nm pores on the surface. The pores contain one or more of carboxyl groups, aromatic groups, hydrophobic bonds, π-π bonds, salt bonds, and hydrogen bond binding sites, and are used to adsorb psychiatric drugs in serum. The adsorption recovery rate is 94% to 113%, and the protein exclusion rate exceeds 95%. The present invention has the advantages of rapid magnetic separation, efficient adsorption of psychiatric drugs in serum, and a high protein exclusion rate, which can simplify the detection process and reduce the detection error caused by protein interference.

The invention aims to provide a method, equipment, medium and program product for determining binding protein information with pH sensitivity, and the method comprises the following steps: determining the binding protein information based on the target protein information, the target amino acid corresponding to the binding region amino acid of the target protein and the noise information of the binding protein in the initial time step; according to a first binding protein generation model, binding protein information corresponding to the target protein is generated, the target amino acid and binding region amino acid of the target protein are matched with preset pH sensitivity information, and binding protein sequence information in the binding protein information contains the target amino acid; the first binding protein generation model includes a first flow matching predictive model. A velocity field from noise data to binding protein information is predicted through a flow matching method, and the pH sensitivity of the binding protein is improved by introducing target amino acid in each step of prediction, so that ideal binding protein information is generated, the experience requirement of a protein designer is reduced, and the design efficiency is improved.

The present disclosure generally relates to compositions and methods for reducing the biological age and / or the rate of biological age increase in adult animals. More specifically, the present disclosure relates to administering a formulation comprising an effective amount of glycine and / or a functional derivative thereof, alone or in combination with a dietary regimen, such as a calorie-restricted diet, an aging diet, or a low-protein diet. The formulation can simultaneously reduce the biological age of a tissue, organ, or physiological system in an adult animal in need thereof; or treat, prevent, or delay the onset of organ disease, or organ function decline, or conditions associated therewith, in an adult animal in need thereof.

The invention provides a natural protein immobilization method. The method comprises the following steps: step 1, reacting a reactant with target natural-state protein; step 2, preparation of a reactant modification interface; and step 3, fully mixing the natural-state protein 5-HTT with the obtained reactant modification interface, stirring at room temperature for 6 hours, and washing with PBS for 3 times to complete high-activity immobilization of the natural-state 5-HTT. Through the proximity effect of ligand-functional protein affinity interaction, a nucleophilic amino acid residue at a specific site of the native protein and a ligand electrophilic group are induced to be subjected to a covalent reaction, the ligand is released through synchronous self-cleavage, and a ligand binding domain is exposed, so that high-activity one-step capture of the native protein is realized; the technical problems of low immobilization efficiency, serious protein activity loss and low specificity of low-abundance natural state functional protein in a complex system are successfully solved.