385 results about "Soybean Phospholipids" patented technology

The invention provides a lipidosome, a preparation method thereof and application of the lipidosome in preparation of a medicine for treating PARP inhibitor-resistant breast cancer, and relates to the technical field of biology. The preparation method of the liposome provided by the invention comprises the following steps: under a stirring condition, injecting an ethanol solution dissolved with soybean phospholipid and cholesterol into a histidine aqueous solution for hydration, volatilizing an ethanol component after the hydration is finished, and carrying out ultrasonic treatment to prepare the liposome. The preparation method is simple and convenient, and the prepared liposome loaded with histidine is high in encapsulation efficiency. The research of the inventor finds that histidine metabolism reprogramming occurs in the PARPi resistance process of breast cancer cells, so that DNA synthesis repair is maintained. And after the PARPi-resistant breast cancer cells are treated by adopting the liposome disclosed by the invention, the histidine content in the breast cancer cells can be increased, so that the PARPi-resistant cells are sensitive to PARPi, and further the treatment on the PARPi-resistant breast cancer is realized.

The invention provides a method for preparing multi-dimensional co-assembled hydroponic oil and application of the multi-dimensional co-assembled hydroponic oil, and belongs to the technical field of grease fermentation. The method comprises the following steps: S1, taking an oil-insoluble substance, mixing the oil-insoluble substance with soybean phospholipid in absolute ethyl alcohol, stirring at 50-70 DEG C until the solution is clear, centrifuging, taking a supernatant, and volatilizing a solvent to obtain a compound; s2, dissolving chitosan and fermentation oil in an acetic acid solution with the concentration of 0.5-2 wt%, uniformly mixing, and standing to obtain a mixed solution; s3, dissolving the compound obtained in the step S1 in absolute ethyl alcohol, slowly dropwise adding into the mixed solution obtained in the step S2 in a constant-speed stirring state, and stirring until the mixed solution emits micro-blue opalescence to obtain a co-assembled solution; and S4, adding the co-assembled solution in the S3 into grease to obtain the multi-dimensional co-assembled hydroponic oil. According to the method, oil-insoluble components can be wrapped and dissolved in grease, the structure is stable, the skin permeation and absorption performance of effective components can be improved, and the grease is endowed with more dermatological functional activity.

The invention discloses a soybean phospholipid disease-preventing and root-promoting microbial agent as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises a microbial composition, modified enzymolysis soybean phospholipid, a carrier and auxiliary components. According to the invention, the modified enzymolysis soybean phospholipid and microorganisms are combined to achieve a synergistic effect. According to the method, soybean phospholipid is modified and subjected to enzymolysis, and the steps of pretreatment, stepped low-temperature treatment, enzymolysis treatment, enzyme inactivation and purification and the like are carried out, so that the quality and performance of the soybean phospholipid are improved, the rhizosphere colonization rate of strains can be increased, the disease prevention and root promotion effects can be improved, the soil micro-ecological environment can be improved, and good growth conditions are created for plant root systems; and the disease-preventing and root-promoting effects of the microbial agent are further enhanced. The microbial agent disclosed by the invention can be used for remarkably reducing the morbidity of wheat sharp eyespot and reducing the use amount of chemical pesticides, can also be applied to planting of other crops, and can play a role in preventing and controlling various plant diseases and promoting root growth.

The invention discloses a preparation method and processing equipment of herbal composite granules, and relates to the technical field of composite granules. Comprising the following components in parts by weight: 15-20 parts of soybean phospholipid, 10-15 parts of glutamine, 10-15 parts of L-arginine, 5-10 parts of a ginseng extract, 5-10 parts of phytosterol, 5-10 parts of xylooligosaccharide, 5-10 parts of inositol, 5-10 parts of a hawthorn extract, 5-10 parts of a blueberry extract, 5-10 parts of an acerola cherry extract, 0-5 parts of yeast beta glucan, 0-5 parts of a sea-buckthorn extract, 0-5 parts of a tartary buckwheat extract, 0-5 parts of haematococcus pluvialis and the like. The composition is innovative and breakthrough in successful application in the aspects of heart and cerebral vessels and anti-aging, and can regulate intestines, protect liver, reduce blood pressure and regulate blood fat; thrombus is melted, the vascular endothelial function is improved, and arteriosclerosis is prevented; meanwhile, the effects of resisting oxidation, resisting inflammation, resisting aging and enhancing immunity can also be achieved.

The invention belongs to the technical field of traditional Chinese medicines and cosmetics, and particularly relates to eutectic resveratrol, a liposome thereof, a preparation method and application of the eutectic resveratrol. The eutectic resveratrol is prepared from the following raw materials: resveratrol, amino acid and glycerol; the amino acid is selected from one of proline, lysine and arginine. The eutecticevaporate resveratrol liposome is prepared from the following raw materials: soybean phospholipid, cholesterol and eutecticevaporate resveratrol, the mass ratio of the soybean phospholipid to the cholesterol is (1-9): 1, and the mass ratio of the sum of the mass of the soybean phospholipid and the cholesterol to the mass of the eutecticevaporate resveratrol is 1: (0.5-2). A method capable of improving the solubility and stability of resveratrol is found, the eutectic resveratrol liposome which is stable in particle size and PDI and excellent in zeta potential and has certain anti-oxidation and antibacterial performance is prepared, the solubility, stability and bioavailability of resveratrol (RES) are improved, and the resveratrol liposome is suitable for being used as an anti-inflammatory drug. And a new thought is provided for further development and application research of resveratrol (RES).

The invention discloses external emulsifiable paste for directionally delivering tranexamic acid to an epidermal layer, which is prepared from the following raw materials in percentage by weight: 1-15% of tranexamic acid, 0.5-7% of alpha-arbutin, 0.3-4% of edetate disodium, 1-5% of azone, 1-5% of sodium dodecyl benzene sulfonate and the balance of water. The skin care product is prepared from, by weight, 0.1%-2% of salicylic acid, 1%-10% of glycerin, 2%-15% of propylene glycol, 0.1%-4% of tween-80 or 0.4%-2% of sorbitan oleate, 0.5%-5% of alpha-tocopherol, 0.5%-5% of diisostearyl alcohol malate, 0.5%-6% of soybean phospholipid, 1%-10% of glycerin monostearate, 0.5%-10% of tetradecanol, 0.02%-0.1% of propyl hydroxybenzoate, 1%-10% of stearic acid, 0.5%-6% of stearyl alcohol and the balance water. Meanwhile, the invention also discloses a preparation method, experiments show that the external cream prepared by the invention has strong capacity of delivering tranexamic acid to the epidermal layer and can ensure that the tranexamic acid can be quickly absorbed by cells to take effect, and the defect that tranexamic acid is difficult to pass through skin cuticle obstacles due to strong hydrophilicity is overcome.

The invention discloses RVG29 peptide modified tripterine transfersome nasal in-situ gel and a preparation method and application thereof.The in-situ gel is mainly prepared from tripterine, soybean phospholipid, cholesterol, mPEG2000-DSPE, mPEG2000-DSPE-RVG29, sodium deoxycholate and DGG, the RVG29 peptide modified tripterine transfersome nasal in-situ gel can increase the solubility of indissolvable drugs, and the RVG29 peptide modified tripterine transfersome nasal in-situ gel has the advantages that the solubility of the indissolvable drugs is improved; and the RVG29 peptide which can be specifically recognized with a dopaminergic neuron high-expression acetylcholine receptor is modified on the surface of a carrier, so that efficient active targeting endocytosis can be effectively realized, and the RVG29 peptide can be used for treating the Parkinson's disease.

To provide a topical skin preparation that effectively promotes collagen production within the skin, exhibits excellent usability, and effectively enhances post-use stratum corneum moisture content.SOLUTION: A topical skin preparation comprises niacinamide, a heparin-like substance, dipotassium glycyrrhizinate, a collagen peptide, a yeast extract, and glycylglycine. It is preferable that the topical skin preparation further contains hydrogenated soybean phospholipids and phytosterols. It is more preferable that the topical skin preparation has a niacinamide content of 5 mass% or more. It is also preferable that the topical skin preparation further contains at least one plant extract selected from Arnica montana and Plantago major.SELECTED DRAWING: Figure 1

The invention provides a ruminant rumen bypass coating preparation and a preparation method and application thereof. The rumen bypass coating preparation comprises a core material and a coating, wherein a coating solution for preparing the coating comprises the following components in parts by weight: 8-10 parts of soybean lecithin oil; 2-3 parts of reducing sugar; 18 to 20 parts of grease; the coating liquid is prepared by the following steps: heating and melting 8-10 parts of soybean lecithin oil at the high temperature of 60-90 DEG C, adding 2-3 parts of reducing sugar into a molten reaction pot, stirring until the reducing sugar is molten, and preserving heat at 50 DEG C after the materials react to be brown yellow; and additionally taking 18-20 parts of grease, heating and melting, mixing the material obtained in the previous step with the grease, and uniformly stirring to form a mixed solution, namely the coating solution. According to the invention, the Maillard reaction between lecithin and reducing sugar is utilized to produce a product with good rumen bypass and intestinal release effects, the obtained product has high purity, good quality and high small intestine release rate, the vitality of immune cells is improved, and the disease resistance and stress resistance are enhanced.

The present application provides a rhodiolide composition with a core-shell-shell structure, a preparation method thereof comprising the steps of: 1) EGCG inclusion: adding beta-cyclodextrin and EGCG into water, ultrasonic, forming an inclusion compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, dissolving in chloroform to form a uniform solution; weighing rhodiolide, dissolving in the uniform solution, rotary evaporation to remove chloroform, then adding the inclusion compound and PBS buffer into the container, hydrating, forming a liposome wrapping rhodiolide and inclusion compound; 3) shell loading: weighing collagen, mixing with chitosan solution uniformly to form a complex; adding the complex into the liposome, the complex is coated on the outer layer of the liposome, centrifugal purification, obtaining a rhodiolide composition with a core-shell-shell structure. The present application belongs to the technical field of cosmetic raw materials, and the rhodiolide composition provided has the advantages of high stability, good transdermal effect, and significant anti-aging effect.

The present invention provides a compound feed for freshwater fish and a preparation method thereof, comprising the following raw materials in parts by weight: 39-44 parts of corn protein powder, 22-34 parts of cottonseed protein powder, 5.4-9.2 parts of black soldier fly larva powder, 11-23 parts of fish oil, 20-30 parts of Schizochytrium powder, 7.5-12.4 parts of soy lecithin powder, 4-10 parts of maltitol, 8-12 parts of sodium butyrate, 8-15 parts of Chinese herbal medicine liquid, 0.2-0.8 parts of compound fermenting agent. Through steps such as raw material mixing, Chinese herbal medicine liquid fermentation, homogenization, puffing and spray drying in sequence, the feed quality control is carried out, so that the compound feed has a relatively high absorption rate and palatability, ensures the intake of the components of the Chinese herbal medicine fermentation liquid, significantly improves the survival rate and growth rate of freshwater fish, and has remarkable economic benefits.

The invention provides crab feed capable of improving the quality of crab cream. The feed is prepared from the following raw materials in parts by weight: 15-25 parts of white fish meal, 10-15 parts of fermented soybean meal, 50-60 parts of squid visceral meal, 45-50 parts of krill meal, 25-30 parts of beer yeast, 200-220 parts of high gluten flour, 45-50 parts of wheat bran, 10-20 parts of soybean lecithin oil, 5-10 parts of a vitamin premix special for crabs and 5-10 parts of a mineral premix special for crabs. According to the crab feed capable of improving the quality of the crab cream, on the basis of ensuring the ingestion and intestinal health of crabs, conditions are created for efficient nutrient absorption and utilization, then gonad development and flavor substance accumulation are driven through key substances, meanwhile, a three-dimensional anti-oxidation and immune protection system runs through all the time, the body health is guaranteed, and the crab feed has the effects of improving the crab cream quality and improving the crab cream quality. The method has the advantages of simple operation, stable color and flavor of the crab cream, sufficient energy supply in the whole process, and synergistic operation of all the systems, so that the disease resistance and survival rate of crabs are improved, and the accumulated nutrient substances are efficiently converted into the plump degree, delicious flavor and attractive color of the crab cream.

The invention discloses a preparation method of waxberry anthocyanin lipidosome, and belongs to the field of waxberry anthocyanin lipidosome. The preparation method of the waxberry anthocyanin lipidosome comprises the following steps: dissolving anthocyanin, cholesterol and soybean lecithin in an organic solvent, and stirring in an ultrasonic crusher to obtain a mixture; and stirring the mixture, transferring the mixture into a pear-shaped bottle, carrying out rotary evaporation, hydrating, and filtering to obtain the waxberry anthocyanin liposome. The waxberry anthocyanin liposome prepared by the method has better stability; moreover, after the preparation conditions of the waxberry anthocyanin liposome are optimized by using a response surface method, the obtained waxberry anthocyanin liposome has relatively high encapsulation efficiency, so that a theoretical basis is provided for development of a novel anthocyanin dosage form and application of the novel anthocyanin dosage form in a health-care food market.

The invention provides a salidroside composition with a core-shell-shell structure, and a preparation method of the salidroside composition comprises the following steps: 1) EGCG (epigallocatechin gallate) clathration: adding beta-cyclodextrin and EGCG into water, and carrying out ultrasonic treatment to form a clathrate compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, and dissolving in chloroform to form a uniform solution; the preparation method comprises the following steps: weighing salidroside, dissolving the salidroside in a uniform solution, carrying out rotary evaporation to remove chloroform, then adding an inclusion compound and a PBS (Phosphate Buffer Solution) into a container, and hydrating to form lipidosome wrapping the salidroside and the inclusion compound; 3) shell loading: weighing collagen, and uniformly mixing the collagen with the chitosan solution to form a compound; the salidroside composition with the core-shell-shell structure is obtained by dropwise adding the compound into the lipidosome, coating the outer layer of the lipidosome with the compound and carrying out centrifugal purification. The invention belongs to the technical field of cosmetic raw materials, and provides a salidroside composition which has the advantages of high stability, good transdermal effect, remarkable anti-aging effect and the like.

The invention provides a multilayer lutein ester liposome as well as a preparation method and application thereof, and belongs to the field of food processing. The multi-layer lutein ester liposome is prepared from the following raw materials: 30 to 50 parts of lutein ester, 40 to 60 parts of soybean phosphatidylcholine, 5 to 20 parts of cholesterol, 10 to 25 parts of starch sodium octenylsuccinate, 20 to 40 parts of trehalose, 15 to 30 parts of mannitol, 4 to 10 parts of phosphatidyl glycerol, 1 to 2 parts of vitamin E acetate and 1 to 2 parts of ascorbyl palmitate. According to the present invention, through the specific food-grade raw material composition and the accurate ratio relationship, the comprehensive delivery system is constructed for the highly hydrophobic and unstable lutein ester, and the system synchronously achieves the high encapsulation efficiency, the excellent stability, the good water dispersibility and the slow release function.

The invention discloses a leontopodium alpinum callus extract as well as a preparation method and application thereof, and belongs to the technical field of plant active ingredient extraction. The leontopodium alpinum callus extract is prepared by the following steps: mixing leontopodium alpinum callus freeze-dried powder with a buffer solution, adding cellulase, pectinase and beta-glucanase, uniformly mixing, reacting to obtain primary enzymolysis slurry, adjusting the primary enzymolysis slurry to be neutral, adding phospholipase A2, stirring, reacting to obtain deep enzymatic hydrolysate, filtering, washing, and drying to obtain the leontopodium alpinum callus extract. Transferring into an extraction kettle, adding an entrainer, and extracting to obtain a leontopodium alpinum extract; dissolving soybean phospholipid and cholesterol in ethanol to obtain an organic phase; dispersing the leontopodium alpinum extract in a buffer solution to obtain an active matter water phase; and injecting the organic phase into the active matter water phase, stirring, carrying out reduced pressure distillation, and homogenizing. The preparation method provided by the invention can efficiently and completely obtain the active component, and the nano-liposome formed by phospholipid molecules can effectively improve the stability of the active component.

The invention relates to the field of traditional Chinese medicine active ingredient new dosage form pharmaceutical preparations, and discloses an ROS responsive bionic nano drug delivery system, a preparation method and application. The preparation method comprises the following steps: taking lipid components, namely soybean phospholipid (SPC), hydrogenated soybean phospholipid (HSPC), dipalmitoyl phosphatidylcholine (DPPC), pegylated distearoyl phosphatidyl ethanolamine (DSPE-PEG), dioleoyl lecithin (DOPC), (2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate) and a surfactant, as well as an emulsifier, as well as a solvent; the ROS-responsive leonurine lipidosome is prepared by taking 1, 3-dioleoyl-propyl)-trimethylamine (DOTAP), cholesterol and the like as raw materials to prepare nano-lipidosome, taking the nano-lipidosome as an inner core and coating the leonurine serving as a traditional Chinese medicine effective component and catalase capable of catalyzing active oxygen to generate oxygen, so as to form the ROS-responsive leonurine lipidosome. And then realizing bionic fusion with an activated neutrophile granulocyte membrane on the outer layer through a pneumatic nano extruder co-extrusion technology, so as to prepare the activated neutrophile granulocyte membrane coated ROS responsive bionic nano-liposome drug delivery system. The invention can realize targeted therapy of rheumatoid arthritis and the like, and has the advantages of biological safety, slow release and the like.

The invention belongs to the field of substance content detection, and discloses a method for determining the content of soybean phosphatidylcholine or phosphatidylethanolamine in a soft capsule, which comprises the following steps: (1) preparing a soybean phosphatidylcholine or phosphatidylethanolamine standard solution; (2) preparing a to-be-detected sample solution; (3) performing high performance liquid chromatography analysis; (4) drawing a standard curve; and (5) calculating the content of soybean phosphatidylcholine or phosphatidyl ethanolamine. The method has the advantages of high response value, short retention time and stable base line, and is not interfered by other components in the soft capsule; the method has the advantages of simple operation, high precision and good repeatability, the recovery rate meets the analysis requirement, and the method can efficiently, quickly and accurately detect the content of two components, namely soybean phosphatidylcholine and phosphatidyl ethanolamine, of soybean phospholipid in the soft capsule content.

The utility model provides an on-line weighing reinspection system comprising a main body assembly, the top of which is provided with a driving assembly. The driving assembly comprises a supporting rod, a controller, a first connecting rod, a first motor, a first threaded rod, a sliding table, a second motor, a second threaded rod, a supporting plate, an electric telescopic rod, a second connecting rod, a third motor, a bidirectional lead screw and a clamping plate. The first motor drives the first threaded rod to rotate to enable the sliding table to move, the electric telescopic rod works to enable the clamping plate to be close to a soybean phospholipid packaging bag, the third motor drives the two-way lead screw to rotate, soybean phospholipid products are clamped through the clamping plate and placed on the weighing device to be weighed, and after weighing and re-inspection are completed, the weighing device is used for weighing the soybean phospholipid products. Qualified products are continuously conveyed along the production line, and unqualified products are conveyed into the storage box, so that the online reinspection work of the soybean phospholipid products is automatically completed, manual operation of workers is not needed, time is saved, and the reinspection efficiency and the reinspection result accuracy are improved.

The invention provides a low-temperature composite oil phase for an emulsion explosive, a preparation method of the low-temperature composite oil phase and the emulsion explosive. The low-temperature composite oil phase for the emulsion explosive comprises the following raw materials in parts by weight: 50-55% of wax special for explosives, 0-6% of engine oil, 1-3% of vaseline, 22-38% of a composite emulsifier and 1-6% of soybean lecithin. The compound emulsifier comprises 1%-5% of an H1507C type polymer emulsifier, 1%-3% of a KL-118b type compound emulsifier and 20%-30% of an Sp-80 emulsifier. According to the low-temperature composite oil phase for the emulsion explosive, the composite emulsifier compounded in the low-temperature composite oil phase is optimized and is comprehensively blended with other components, so that the oil film stability of the emulsion explosive can be remarkably enhanced, and the emulsion explosive with high stability can be prepared.

The present invention belongs to the field of soybean by-product processing and discloses a method for preparing high-quality transparent soybean lecithin, comprising the following steps: (1) a dual solvent system 1: dissolving soybean lecithin in a non-polar solvent, then adding a certain amount of monohydric alcohol aqueous solution and stirring to obtain a mixed solution 1; (2) centrifuging the mixed solution 1 to remove impurities and collecting the upper non-polar solvent clear liquid; (3) a dual solvent system 2: adding a certain amount of polyol to the collected upper non-polar solvent clear liquid and stirring to obtain a mixed solution 2; (4) centrifuging the mixed solution 2 to remove impurities and water, collecting the upper non-polar solvent clear liquid, and recovering the non-polar solvent to obtain transparent soybean lecithin. The method disclosed in the present invention has the advantages of simple process, low equipment requirements, and solvent recyclability. The prepared soybean lecithin has extremely high transparency and good fluidity, and no substances such as proteins and polysaccharides that affect the transparency of the soybean lecithin are detected.

The utility model discloses a soybean lecithin oil powder mixing device which comprises a mixing barrel, a barrel cover is installed on the mixing barrel, a stirring motor is installed on the barrel cover, a main stirring shaft and an auxiliary stirring shaft are arranged in the mixing barrel, the main stirring shaft is rotationally connected to the center of the auxiliary stirring shaft, a driving gear is fixed to the top end of the main stirring shaft, and a driving motor is installed on the driving gear. A gear ring is installed at the top end of the auxiliary stirring shaft, linkage gears are connected to the sides, close to each other, of the gear ring and the driving gear in a meshed mode, chain wheels are fixed to the bottom ends of the two linkage gears, and the two chain wheels are in transmission connection through a chain in a sleeving mode; and the main stirring shaft and the auxiliary stirring shaft are controlled to rotate reversely. According to the utility model, the main stirring shaft and the auxiliary stirring shaft are arranged in the mixing barrel, the main stirring shaft and the auxiliary stirring shaft can rotate in opposite directions for stirring, so that the caking condition of oil powder during stirring is reduced, and compared with the existing homodromous rotation stirring, the stirring is more sufficient, and the mixing effect is ensured.

This invention relates to the field of baking food processing technology, and discloses a method for preparing sponge cake using an oil gel as a substitute for butter. The method includes heating and stirring refined rapeseed oil and food-grade candelilla wax to obtain a lipid eutectic; after cooling, adding food-grade soybean lecithin and shearing and mixing; mechanically shearing and cooling to 45°C to obtain a microcrystalline network primary material; obtaining a modified oil gel through low-speed stirring, slow rewarming and constant temperature, and then cooling to 25°C and holding at that temperature; using this oil gel to replace butter and mixing with baking ingredients at low speed and forced shearing and whipping to obtain a batter; continuously baking in molds and naturally cooling and demolding to obtain a sponge cake. This invention utilizes lecithin and wax crystals to synergistically construct a three-dimensional network framework with thixotropic recovery capabilities, solving the defects of conventional oil gel networks that are fragile and easily damaged by shearing. It effectively traps air bubbles and prevents oil precipitation during the batter whipping stage, reducing saturated fat intake while resulting in a soft, porous sponge cake with no residual wax.

The invention discloses a preparation method of zoeae bait for blue crab artificial fry. The method comprises the following steps: weighing the following raw materials in percentage by weight: 31%-42% of muscula seeking, 29%-33% of hairtail, 15%-20% of metapenaeus vannamei and 5%-25% of nutritional materials; the nutritional material comprises fish meal, cuttlefish paste, soybean meal, high gluten flour, composite vitamins, a modified soybean phospholipid emulsifier, a dark plum extract and turmeric powder; the preparation method comprises the following steps: adding seawater with the same weight as the musculus seeking and the hairtail into the musculus seeking and the hairtail, crushing into a first clastic liquid through a crusher, then adding the miripenaeus vannamei into the first clastic liquid, and uniformly stirring to obtain a second clastic liquid; and adding compound protease and the like which are 0.33-0.45% of the weight of the second clastic liquid into the second clastic liquid.

The invention belongs to the field of soybean by-product processing, and discloses a method for preparing high-purity soybean phospholipid, which comprises the following steps: completely dissolving soybean concentrated phospholipid with an extractant, continuously adding the extractant, stopping adding the extractant after 30-50 wt% of precipitates are separated out, separating to obtain the precipitates and a mixed solution, standing the mixed solution, collecting the clarified mixed solution and the bottom precipitates, combining the precipitates to obtain soybean phospholipid mixture A; recovering the extractant in the clarified mixed solution to obtain soybean phospholipid mixture B, adding an ethanol aqueous solution, stirring until it becomes paste-like, adding an alkane solvent, stopping stirring after mixture B is completely dissolved, recovering the alkane solvent, soybean phospholipid mixture B is divided into two layers, removing the upper layer to obtain soybean phospholipid mixture C, removing water, and mixing it with soybean phospholipid mixture A. The acetone-insoluble matter content in soybean phospholipid mixture A is >95%, and the acetone-insoluble matter content in soybean phospholipid mixture C is >90%.

The application provides the technical field of medical materials and particularly relates to a kind of ultra-thin high-strength nanofiber medicinal casing and a preparation method thereof.The preparation method comprises the following steps: (1) preparing pretreated raw materials; (2) adding a complex enzyme to the pretreated raw materials for enzymolysis, enzymolysis at 35-40 DEG C for 5-8 h, enzyme inactivation, to obtain an enzymolysis product; (3) adding a complex bacteria to the enzymolysis product for anaerobic fermentation, fermentation at 35-40 DEG C for 10-12 h, sterilization, to obtain a fermentation product; (4) rinsing the fermentation product with clean water for 5-8 times, to obtain a casing semi-finished product; (5) mixing nanocellulose, soybean phospholipid and water to prepare a liquid, spraying the liquid on the casing semi-finished product, drying, to obtain an ultra-thin high-strength nanofiber medicinal casing.The casing prepared by the application can be used to wrap traditional Chinese medicine compositions.

The invention relates to an HPLC-UV (High Performance Liquid Chromatography-Ultraviolet) detection method for components of amphotericin B lipidosome, which comprises the following steps: S1, providing a chromatographic column of which a stationary phase is a YMC Triart C18 column, regulating the pH (Potential of Hydrogen) of a mobile phase in the chromatographic column to 9 to 11 by using n-butylamine or triethylamine, and isocratically eluting the amphotericin B lipidosome at the temperature of 28 to 32 DEG C; s2, carrying out qualitative and quantitative analysis on sodium stearoyl phosphatidyl glycerol, hydrogenated soybean phosphatidylcholine, cholesterol and tocopherol in the amphotericin B liposome in an ultraviolet region of 200-240nm by virtue of HPLC-UV (High Performance Liquid Chromatography-Ultraviolet) on the basis of a reversed-phase partition chromatography principle; wherein the phase A of the mobile phase gradient is a mixture of methanol and acetonitrile, the phase B of the mobile phase gradient is ammonium acetate contained in water, and the volume ratio of the phase A to the phase B is 96: 4. The detection method provided by the invention can be used for simultaneously analyzing phospholipid, tocopherol and cholesterol in the amphotericin B lipidosome.

The invention discloses a W / O / W type nano-emulsion loaded with cocaine esterase as well as a preparation method and application of the W / O / W type nano-emulsion. The nano-emulsion is prepared from cocaine esterase, a phosphate buffer solution, soybean phospholipid, vitamin E polyethylene glycol succinate, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and medium chain triglyceride. The preparation method comprises the following steps: preparing the inner water phase; preparing an oil phase; slowly injecting the internal water phase into the oil phase under shearing to obtain W / O type primary emulsion; slowly adding the primary emulsion into the external water phase under shearing, and performing high-speed shearing to obtain a secondary emulsion; and performing high-pressure homogenization on the secondary emulsion to obtain the final W / O / W type nano-emulsion. The cocaine esterase is combined with the nano-emulsion for the first time, and the nano-emulsion prepared by the method has the effects of improving the temperature stability of the cocaine esterase and prolonging the in-vivo half-life period, and can be applied to rescue or prevention of cocaine acute poisoning.

The invention discloses a slow-release coenzyme Q10 liposome and a preparation method thereof. The liposome comprises a coenzyme Q10 core material and a double-layer wall material, the double-layer wall material comprises an inner-layer wall material and an outer-layer composite wall material; the inner-layer wall material is prepared from at least one of hydroxypropyl methyl cellulose, pectin, gelatin and xanthan gum; the outer-layer composite wall material is a combination of modified soybean phospholipid and a first wall material; the first wall material is selected from at least one of the following materials: maltodextrin, starch sodium octenylsuccinate, Arabic gum and cyclodextrin. The coenzyme Q10 lipidosome with high content and high stability is prepared by adopting a double-layer inclusion technology and a lipidosome technology, the content of the lipidosome coenzyme Q10 can reach up to 50%, and the lipidosome has good dispersion performance in water, has continuous oxidation resistance, and can effectively neutralize free radicals and relieve oxidative stress. The effective release and retention of the coenzyme Q10 under the condition of simulating intestinal tracts can be maintained, and the stability and the bioavailability of the CoQ10 are enhanced.

The invention discloses soybean phospholipids and an extraction method thereof, and belongs to the technical field of soybean phospholipids, the extraction method of soybean phospholipids comprises the following steps: selecting fresh soybean oil leftovers as an extraction raw material, carrying out centrifugation, acetone deoiling and ethanol extraction to obtain an ethanol extraction liquid, finally adding attapulgite composite cross-linked chitosan microspheres into the ethanol extraction liquid, and carrying out centrifugation, acetone deoiling and ethanol extraction to obtain the soybean phospholipids. And further removing impurity molecules to obtain the soybean phospholipid with high stability and high soybean lecithin content. According to the present invention, by using the multiple pore structures and the electrostatic interaction of the attapulgite composite cross-linked chitosan microspheres, the impurity macromolecules and the polar inositol phospholipid, cephalin and other compounds having competitive dissolution with the soybean lecithin are separated so as to increase the soybean lecithin content in the soybean lecithin; moreover, tea polyphenol is also grafted on the attapulgite composite cross-linked chitosan microspheres, so that free radicals can be cleared through a hydrogen supply mechanism, the phospholipid oxidation process is blocked, the dual functions of high stability and high antioxidant activity are realized, and the quality of the obtained soybean phospholipid is improved.